Click here to switch to the map view.

The map label for this gene is nadB [C]

Identifier: 158421746

GI number: 158421746

Start: 147793

End: 149415

Strand: Reverse

Name: nadB [C]

Synonym: AZC_0122

Alternate gene names: 158421746

Gene position: 149415-147793 (Counterclockwise)

Preceding gene: 158421747

Following gene: 158421745

Centisome position: 2.78

GC content: 71.97

Gene sequence:

>1623_bases
ATGACGATCCAGATTCCCTATGCCGCGGCGCTCGCCGCCATCGCGTCCGGTTCGGCTCCGCCGGTCCGCAGCGACGACCT
TCCGGTCGAGACGCTCCTGGCCCGCTTCCATCCCGACCATGGGCCGCGGGCGCGGGTGCCGCTGCGCATCGGCGCCAACG
CCGGTGACGCCTGCCACCCGGAACTCGCCCGGCTGCTGGAGAGCAATGCGCTGATCGACGATCTCGACCTCGCGCGCGCG
ACGCCTCTTGCGACGGACGTGCTGGTCATCGGCGGCGGCGGCGCGGGCGCGGCGGCGGCCGTCACGGCGGCGCGGCAGGG
CGCGCGCGTCATCATCGCCGGCAAGCTGCGCATCGGCGACAGCAATACGGTGATGGCGGAGGGCGGCATCCAGGCCGCGG
TCGGCGCGGAGGACAGCTTGCAGCGCCATTTCGACGACACCGTGCGCGGCGGGCACGGCCGGGGCGAGCACATGCTCATC
GCGCAGATGGTGCGCGATGCGCCGGATGTGATCCGCTGGCTCATCGACCTCGGCATGGCTTTCGATCTCTCGCAGGACAA
GGCCCTCGGCCAGACGCTGGTGCGGAAGCGGGCCGGCGGCACCACGGCGGCGCGTATCCTGTGCCACCGCGACTTCACCG
GGCTCGAGATGATGCGCGTGTTGCGCGAGGCGGTGGAACTACAAGGCGGCATCGAGCAGTTCATGCGATGCCCCGCCGTG
GAGCTGCTGTCGCACGAGGACGGCAGTTGCGCCGGTGCGGTCCTGTACGATCTGGAGCGGCGCAGCTTCCTGCTGGTGCG
CGCCAACGCCGTCGTGCTGGCGACCGGCGGAAGCGGCCGGCTGCACCTCCAGGGCTTCCCGACCTCCAACCATTACGGCG
CGACGGCAGACGGCCTCGTGCTGGCCTATCGGCTGGGCGCGCGGATGCGGGATCTGGATTCCTTCCAGTATCACCCGACC
GGCGTCGCCTGGCCGCGCCATCTGGCGGGCGGCCTGATCTCGGAGGCCGCACGCTCGGCCGGCGCCGTGTTGCTCAACGG
CCTCGGAGAGCGCTTCGTGGACGAGCTCCAGCCCCGCGACGTGGTGGCCGCCGCGATCCTGCGCGAATGCGCGGAGCAGC
GCGGCATCATGCGGGACGGGGAGACGGGCGTCTTCCTCGACGTGCGCGGTGCGCTCGCCGCGCGGCCGGACCTGCTGGAG
CGCGATCTCGTTTCGCTCGGGCATCTGGCCGCAAAGTGCGGTCTCGACCCGAGGACCGAGCCTTTCCTCATCTATCCGAC
GCTCCACTATCAGAATGGCGGCATCGAGATCGACGGCGACGGGGCAACCGCCGTGCCCGGCCTGTTCTGCGCCGGCGAAG
TCACCGGCGGCATCCACGGCCGCAACCGTCTGATGGGCAACGCCCTGCTCGACATCATCAGCTTCGGCCGGCGGGCCGGC
GCGCGCGCCGCCGAAGCGAGGCCGCCCCACGCGCCGGGGCGCGGCGGCCTCTCCCATGTCCACCGCTGGCGGCGGGAACT
GGCGCGGGCGGGGCTGCCGGCGGATCTGCGCGCCCCGCAGCTCTATCCCACCTACGGCAATTTCGCTCTGCGCCGGGGCA
CCACAGACAGGGGGAACCCATGA

Upstream 100 bases:

>100_bases
GATCTCATGCGGCGCCTGCGGGACATCGAGACCGGCGCCATGACCGTCGAAGGCCCGCAGCCGGCCACCGCATCCGGCGA
ACCGGCGGAAGGATGAGCCC

Downstream 100 bases:

>100_bases
CCTCGCTCAATCAGATCCTGCTGAATCCCGCCATCCAGCTCGGGGCGGACCTCGACGGATGGTTCGCCGCCGGCGGCGGG
CTGGGCCTCGACGCCGCCCT

Product: L-aspartate oxidase

Products: oxaloacetate; NH3; H2O2; iminoaspartate [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MTIQIPYAAALAAIASGSAPPVRSDDLPVETLLARFHPDHGPRARVPLRIGANAGDACHPELARLLESNALIDDLDLARA
TPLATDVLVIGGGGAGAAAAVTAARQGARVIIAGKLRIGDSNTVMAEGGIQAAVGAEDSLQRHFDDTVRGGHGRGEHMLI
AQMVRDAPDVIRWLIDLGMAFDLSQDKALGQTLVRKRAGGTTAARILCHRDFTGLEMMRVLREAVELQGGIEQFMRCPAV
ELLSHEDGSCAGAVLYDLERRSFLLVRANAVVLATGGSGRLHLQGFPTSNHYGATADGLVLAYRLGARMRDLDSFQYHPT
GVAWPRHLAGGLISEAARSAGAVLLNGLGERFVDELQPRDVVAAAILRECAEQRGIMRDGETGVFLDVRGALAARPDLLE
RDLVSLGHLAAKCGLDPRTEPFLIYPTLHYQNGGIEIDGDGATAVPGLFCAGEVTGGIHGRNRLMGNALLDIISFGRRAG
ARAAEARPPHAPGRGGLSHVHRWRRELARAGLPADLRAPQLYPTYGNFALRRGTTDRGNP

Sequences:

>Translated_540_residues
MTIQIPYAAALAAIASGSAPPVRSDDLPVETLLARFHPDHGPRARVPLRIGANAGDACHPELARLLESNALIDDLDLARA
TPLATDVLVIGGGGAGAAAAVTAARQGARVIIAGKLRIGDSNTVMAEGGIQAAVGAEDSLQRHFDDTVRGGHGRGEHMLI
AQMVRDAPDVIRWLIDLGMAFDLSQDKALGQTLVRKRAGGTTAARILCHRDFTGLEMMRVLREAVELQGGIEQFMRCPAV
ELLSHEDGSCAGAVLYDLERRSFLLVRANAVVLATGGSGRLHLQGFPTSNHYGATADGLVLAYRLGARMRDLDSFQYHPT
GVAWPRHLAGGLISEAARSAGAVLLNGLGERFVDELQPRDVVAAAILRECAEQRGIMRDGETGVFLDVRGALAARPDLLE
RDLVSLGHLAAKCGLDPRTEPFLIYPTLHYQNGGIEIDGDGATAVPGLFCAGEVTGGIHGRNRLMGNALLDIISFGRRAG
ARAAEARPPHAPGRGGLSHVHRWRRELARAGLPADLRAPQLYPTYGNFALRRGTTDRGNP
>Mature_539_residues
TIQIPYAAALAAIASGSAPPVRSDDLPVETLLARFHPDHGPRARVPLRIGANAGDACHPELARLLESNALIDDLDLARAT
PLATDVLVIGGGGAGAAAAVTAARQGARVIIAGKLRIGDSNTVMAEGGIQAAVGAEDSLQRHFDDTVRGGHGRGEHMLIA
QMVRDAPDVIRWLIDLGMAFDLSQDKALGQTLVRKRAGGTTAARILCHRDFTGLEMMRVLREAVELQGGIEQFMRCPAVE
LLSHEDGSCAGAVLYDLERRSFLLVRANAVVLATGGSGRLHLQGFPTSNHYGATADGLVLAYRLGARMRDLDSFQYHPTG
VAWPRHLAGGLISEAARSAGAVLLNGLGERFVDELQPRDVVAAAILRECAEQRGIMRDGETGVFLDVRGALAARPDLLER
DLVSLGHLAAKCGLDPRTEPFLIYPTLHYQNGGIEIDGDGATAVPGLFCAGEVTGGIHGRNRLMGNALLDIISFGRRAGA
RAAEARPPHAPGRGGLSHVHRWRRELARAGLPADLRAPQLYPTYGNFALRRGTTDRGNP

Specific function: Catalyzes The Oxidation Of L-Aspartate To Iminoaspartate. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=425, Percent_Identity=32.9411764705882, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1788928, Length=421, Percent_Identity=32.3040380047506, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1786942, Length=425, Percent_Identity=34.8235294117647, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790597, Length=397, Percent_Identity=33.5012594458438, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17550100, Length=399, Percent_Identity=33.3333333333333, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17505833, Length=408, Percent_Identity=32.3529411764706, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI71986328, Length=472, Percent_Identity=27.3305084745763, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322701, Length=437, Percent_Identity=32.7231121281465, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322416, Length=443, Percent_Identity=32.7313769751693, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6320788, Length=481, Percent_Identity=29.7297297297297, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6322511, Length=483, Percent_Identity=24.4306418219462, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137288, Length=440, Percent_Identity=35.2272727272727, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24655642, Length=440, Percent_Identity=35.2272727272727, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24655647, Length=440, Percent_Identity=35.2272727272727, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24663005, Length=454, Percent_Identity=30.3964757709251, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.4.3.16 [C]

Molecular weight: Translated: 57344; Mature: 57213

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQIPYAAALAAIASGSAPPVRSDDLPVETLLARFHPDHGPRARVPLRIGANAGDACHP
CEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCH
ELARLLESNALIDDLDLARATPLATDVLVIGGGGAGAAAAVTAARQGARVIIAGKLRIGD
HHHHHHHCCCCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEEECC
SNTVMAEGGIQAAVGAEDSLQRHFDDTVRGGHGRGEHMLIAQMVRDAPDVIRWLIDLGMA
CCEEEECCCCEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
FDLSQDKALGQTLVRKRAGGTTAARILCHRDFTGLEMMRVLREAVELQGGIEQFMRCPAV
CCCCCHHHHHHHHHHHHCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELLSHEDGSCAGAVLYDLERRSFLLVRANAVVLATGGSGRLHLQGFPTSNHYGATADGLV
HHHCCCCCCCHHHHHEECCCCEEEEEEECEEEEEECCCCEEEEEECCCCCCCCCCCCHHH
LAYRLGARMRDLDSFQYHPTGVAWPRHLAGGLISEAARSAGAVLLNGLGERFVDELQPRD
HHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
VVAAAILRECAEQRGIMRDGETGVFLDVRGALAARPDLLERDLVSLGHLAAKCGLDPRTE
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
PFLIYPTLHYQNGGIEIDGDGATAVPGLFCAGEVTGGIHGRNRLMGNALLDIISFGRRAG
CEEEEEEEEECCCCEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ARAAEARPPHAPGRGGLSHVHRWRRELARAGLPADLRAPQLYPTYGNFALRRGTTDRGNP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
TIQIPYAAALAAIASGSAPPVRSDDLPVETLLARFHPDHGPRARVPLRIGANAGDACHP
EEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCH
ELARLLESNALIDDLDLARATPLATDVLVIGGGGAGAAAAVTAARQGARVIIAGKLRIGD
HHHHHHHCCCCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEEECC
SNTVMAEGGIQAAVGAEDSLQRHFDDTVRGGHGRGEHMLIAQMVRDAPDVIRWLIDLGMA
CCEEEECCCCEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
FDLSQDKALGQTLVRKRAGGTTAARILCHRDFTGLEMMRVLREAVELQGGIEQFMRCPAV
CCCCCHHHHHHHHHHHHCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELLSHEDGSCAGAVLYDLERRSFLLVRANAVVLATGGSGRLHLQGFPTSNHYGATADGLV
HHHCCCCCCCHHHHHEECCCCEEEEEEECEEEEEECCCCEEEEEECCCCCCCCCCCCHHH
LAYRLGARMRDLDSFQYHPTGVAWPRHLAGGLISEAARSAGAVLLNGLGERFVDELQPRD
HHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
VVAAAILRECAEQRGIMRDGETGVFLDVRGALAARPDLLERDLVSLGHLAAKCGLDPRTE
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
PFLIYPTLHYQNGGIEIDGDGATAVPGLFCAGEVTGGIHGRNRLMGNALLDIISFGRRAG
CEEEEEEEEECCCCEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ARAAEARPPHAPGRGGLSHVHRWRRELARAGLPADLRAPQLYPTYGNFALRRGTTDRGNP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: L-Aspartate [C]

Kcat value (1/min): 2 [C]

Specific activity: NA

Km value (mM): 4.1 {L-aspartate}} 0.5 {L-aspartate}} [C]

Substrates: @ASPA02txt*L-Aspartate!; H2O; O2; L-aspartate [C]

Specific reaction: @ASOA02.txt*L-Aspartate! + H2O + O2 = oxaloacetate + NH3 + H2O2 O2 + L-aspartate = H2O2 + iminoaspartate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: Iodoacetate; L-Aspartate; Mesotartrate; NAD+; Tetranitromethane; Urea [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]