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The map label for this gene is nuoF2 [H]

Identifier: 158421745

GI number: 158421745

Start: 146543

End: 147796

Strand: Reverse

Name: nuoF2 [H]

Synonym: AZC_0121

Alternate gene names: 158421745

Gene position: 147796-146543 (Counterclockwise)

Preceding gene: 158421746

Following gene: 158421741

Centisome position: 2.75

GC content: 69.7

Gene sequence:

>1254_bases
ATGACCTCGCTCAATCAGATCCTGCTGAATCCCGCCATCCAGCTCGGGGCGGACCTCGACGGATGGTTCGCCGCCGGCGG
CGGGCTGGGCCTCGACGCCGCCCTTGCCGATCCCGGCGCCATCATCCCGGCGCTGGAGGCCGCAGGCCTGCGCGGCATGG
GCGGCGCCGGCTTTCCCACCCACCGCAAGTGGGCCGCCGCCGCGGCGGCGCAGAACACCGACCGGACCGTGGTCTGCAAC
GGCAATGAGGACGAGCCCGGCACCTTCAAGGATCGCCATCTGCTGCGCTGGACGCCCCATCAGGTGATCGAGGGCGCGCT
GATCGCGGCACTGGCCATCGGCGCCCGGCGCGTCTTCCTCTATATCAACCCCCAGCAGATCGAGGCGTTGGCGGAGATGC
GCCGCGCCGTCGCGCAATGGGCGGATACCGATCGCCTGCGCGCGGTGGAGCGGCGGATCGGAGCGACCCTCGAACTCACC
GTCTTCCCCACATCGGGCCGCTATATCGGCGGCGAGGAGACGGCGGTCATCTCGAGCCTCGAAGGCGGCTTTCCCTTTCC
GCGCCGCAAGCCGCCCTTTCCGGCGGAAAGCGGCTATCGCGGCACCCCGACGCTCATCAACAACACCGAGACGCTCGCCA
ACGTACCCCACATCCTGCGCCACGGTGCAGCGTGGTATCGGCGGCTCGGCCTCGGGGAGGCGGTGGGCACGAAGCTCTAT
TCCCTCTCGGGCGACGTGCTTCGCCCGGGGCTCTACGAGCTGCCCATGGGCACGCCGCTCCAGGACCTGATCTTCGTCCA
CGGGGGCGGCATGCTGGAGGGCAAGGCCTTCAAGGCGGTGTTCACCGGCGGCCCCTCCAACACGCTGCTCACCGCCCGCG
ACCTGGACGTCGCCCTCGACTTCGATTCCGTGCGGGCGCGCCGCTCCCGTCTCGGCACCGGGGCGATGATCGTCATTTCG
GAAGGCACCAGCATCGTGCGCAAGGTGGCGGAATATATCGACTTCTTCGCCAAGGGCTCGTGCGGACAGTGCCCACCCTG
CAAGGCGGGGACGTTCCAGATGGCGCGGCTGCTGGACCGGGTGGACAGCGGGCGTGCCGTGCCGGCGGATCTCGCCGCCC
TCTACAACCTCTGCCAGATCCTTCCGGGCAGCGGCCGCTGCGGGCTGATCGACGGGGCCGTGACGGTGGTGGAGAGCTCG
CTCCATACGTTCCCGGCCGAATACCGGATCGGCGAGCCGTCACCCGTGCCCTGA

Upstream 100 bases:

>100_bases
TGGCGCGGGCGGGGCTGCCGGCGGATCTGCGCGCCCCGCAGCTCTATCCCACCTACGGCAATTTCGCTCTGCGCCGGGGC
ACCACAGACAGGGGGAACCC

Downstream 100 bases:

>100_bases
GCATGCGGTCCGCTCCGGCGGTCTTCTTCACCAAAGCAAGCGGCGCCCCGGAGGCGCCGCTTTTCGTTTGAGCCAGACAT
GTTGGCGCGTAAAAGGCGGA

Product: NADH dehydrogenase

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTSLNQILLNPAIQLGADLDGWFAAGGGLGLDAALADPGAIIPALEAAGLRGMGGAGFPTHRKWAAAAAAQNTDRTVVCN
GNEDEPGTFKDRHLLRWTPHQVIEGALIAALAIGARRVFLYINPQQIEALAEMRRAVAQWADTDRLRAVERRIGATLELT
VFPTSGRYIGGEETAVISSLEGGFPFPRRKPPFPAESGYRGTPTLINNTETLANVPHILRHGAAWYRRLGLGEAVGTKLY
SLSGDVLRPGLYELPMGTPLQDLIFVHGGGMLEGKAFKAVFTGGPSNTLLTARDLDVALDFDSVRARRSRLGTGAMIVIS
EGTSIVRKVAEYIDFFAKGSCGQCPPCKAGTFQMARLLDRVDSGRAVPADLAALYNLCQILPGSGRCGLIDGAVTVVESS
LHTFPAEYRIGEPSPVP

Sequences:

>Translated_417_residues
MTSLNQILLNPAIQLGADLDGWFAAGGGLGLDAALADPGAIIPALEAAGLRGMGGAGFPTHRKWAAAAAAQNTDRTVVCN
GNEDEPGTFKDRHLLRWTPHQVIEGALIAALAIGARRVFLYINPQQIEALAEMRRAVAQWADTDRLRAVERRIGATLELT
VFPTSGRYIGGEETAVISSLEGGFPFPRRKPPFPAESGYRGTPTLINNTETLANVPHILRHGAAWYRRLGLGEAVGTKLY
SLSGDVLRPGLYELPMGTPLQDLIFVHGGGMLEGKAFKAVFTGGPSNTLLTARDLDVALDFDSVRARRSRLGTGAMIVIS
EGTSIVRKVAEYIDFFAKGSCGQCPPCKAGTFQMARLLDRVDSGRAVPADLAALYNLCQILPGSGRCGLIDGAVTVVESS
LHTFPAEYRIGEPSPVP
>Mature_416_residues
TSLNQILLNPAIQLGADLDGWFAAGGGLGLDAALADPGAIIPALEAAGLRGMGGAGFPTHRKWAAAAAAQNTDRTVVCNG
NEDEPGTFKDRHLLRWTPHQVIEGALIAALAIGARRVFLYINPQQIEALAEMRRAVAQWADTDRLRAVERRIGATLELTV
FPTSGRYIGGEETAVISSLEGGFPFPRRKPPFPAESGYRGTPTLINNTETLANVPHILRHGAAWYRRLGLGEAVGTKLYS
LSGDVLRPGLYELPMGTPLQDLIFVHGGGMLEGKAFKAVFTGGPSNTLLTARDLDVALDFDSVRARRSRLGTGAMIVISE
GTSIVRKVAEYIDFFAKGSCGQCPPCKAGTFQMARLLDRVDSGRAVPADLAALYNLCQILPGSGRCGLIDGAVTVVESSL
HTFPAEYRIGEPSPVP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=376, Percent_Identity=37.2340425531915, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI20149568, Length=376, Percent_Identity=37.2340425531915, Blast_Score=240, Evalue=2e-63,
Organism=Escherichia coli, GI1788620, Length=401, Percent_Identity=39.9002493765586, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17531727, Length=370, Percent_Identity=37.8378378378378, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI19920794, Length=370, Percent_Identity=37.2972972972973, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI45550452, Length=356, Percent_Identity=37.3595505617978, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24653819, Length=373, Percent_Identity=36.1930294906166, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24653817, Length=339, Percent_Identity=35.9882005899705, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 44382; Mature: 44251

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLNQILLNPAIQLGADLDGWFAAGGGLGLDAALADPGAIIPALEAAGLRGMGGAGFPT
CCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHCCCCCHHHHHHHCCCCCCCCCCCCC
HRKWAAAAAAQNTDRTVVCNGNEDEPGTFKDRHLLRWTPHQVIEGALIAALAIGARRVFL
CHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCEEEEE
YINPQQIEALAEMRRAVAQWADTDRLRAVERRIGATLELTVFPTSGRYIGGEETAVISSL
EECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEEEECCCCEECCCCHHHHHHC
EGGFPFPRRKPPFPAESGYRGTPTLINNTETLANVPHILRHGAAWYRRLGLGEAVGTKLY
CCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SLSGDVLRPGLYELPMGTPLQDLIFVHGGGMLEGKAFKAVFTGGPSNTLLTARDLDVALD
CCCCCHHCCCCEECCCCCCHHHEEEEECCCEECCCEEEEEEECCCCCCEEEEECCEEEEC
FDSVRARRSRLGTGAMIVISEGTSIVRKVAEYIDFFAKGSCGQCPPCKAGTFQMARLLDR
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
VDSGRAVPADLAALYNLCQILPGSGRCGLIDGAVTVVESSLHTFPAEYRIGEPSPVP
HCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSLNQILLNPAIQLGADLDGWFAAGGGLGLDAALADPGAIIPALEAAGLRGMGGAGFPT
CCHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHCCCCCHHHHHHHCCCCCCCCCCCCC
HRKWAAAAAAQNTDRTVVCNGNEDEPGTFKDRHLLRWTPHQVIEGALIAALAIGARRVFL
CHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCEEEEE
YINPQQIEALAEMRRAVAQWADTDRLRAVERRIGATLELTVFPTSGRYIGGEETAVISSL
EECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEEEECCCCEECCCCHHHHHHC
EGGFPFPRRKPPFPAESGYRGTPTLINNTETLANVPHILRHGAAWYRRLGLGEAVGTKLY
CCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SLSGDVLRPGLYELPMGTPLQDLIFVHGGGMLEGKAFKAVFTGGPSNTLLTARDLDVALD
CCCCCHHCCCCEECCCCCCHHHEEEEECCCEECCCEEEEEEECCCCCCEEEEECCEEEEC
FDSVRARRSRLGTGAMIVISEGTSIVRKVAEYIDFFAKGSCGQCPPCKAGTFQMARLLDR
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
VDSGRAVPADLAALYNLCQILPGSGRCGLIDGAVTVVESSLHTFPAEYRIGEPSPVP
HCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]