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The map label for this gene is adhI [H]

Identifier: 158421729

GI number: 158421729

Start: 124230

End: 125357

Strand: Reverse

Name: adhI [H]

Synonym: AZC_0105

Alternate gene names: 158421729

Gene position: 125357-124230 (Counterclockwise)

Preceding gene: 158421730

Following gene: 158421728

Centisome position: 2.33

GC content: 66.13

Gene sequence:

>1128_bases
ATGAAGTCGCGCGCCGCTGTCGCCTGGGAAGCCAAGAAGCCGCTCACCATCGAAACCATCGAGATCGGCGGGCCGAAGGC
CGGCGAGGTGCTGGTGGAGATCATGGCCACCGGCGTCTGCCACACCGACGCCTACACGCTCGACGGCCTGGATTCCGAGG
GCAAGTTCCCCGCCATCCTCGGCCATGAGGGCGCGGGCATCGTGCGCGAGGTGGGCGCCGGTGTCACCACGCTGAAGGTG
GGCGACCACGTGATCCCGCTCTACACGCCCGAGTGCCGGCAGTGCAAAACCTGCCTCTCCCAACGCTCGAACCTGTGCAC
CTCCATCCGCGCCACGCAGGGCCAGGGCCTGATGCCCGACCGCACCTCGCGCTTCTCCTGCGAACCCGTGAATGGTCGAG
GCGGCGAGATCTTCCATTACATGGGCTGCTCGACCTTCTCGAACTTCACCGTGCTGCCGGAGATCGCGCTGGCCAAAGTC
CGCGAGGACGCCCCCTTCGACAAGATCTGCTACATCGGCTGCGGCGTCACCACCGGCATCGGCGCGGTGGTCTACACCGG
CAAGGTGTGGCCGGGTGCCAATGTCGCGGTGTTCGGCCTCGGCGGCATCGGCCTCAACGTGATCCAGGGTGCCCGCATGG
TGGGCGCCGACAAGATCATCGGCATCGACATCAACCCGGCCAAGGTGGAGATGGCGAAGAAGTTCGGCATGACCGACTTC
ATCAATCCGCTGGAGGTGGGCTCCGACAAGGTGGTCTCGGCCATCCAGGACCTGACCGACGGCGGCGCCGACTTCACCTT
CGACTGCACCGGCAACGTCACCGTGATGCGCCAGGCGCTGGAAGCCTGCCATCGCGGCTGGGGCACCTCCATCGTCATCG
GCGTGGCGCCGGCGGGGGCGGAAATCTCCACCCGTCCGTTCCAGCTCGTCACCGGCCGCAACTGGCGCGGCTCCGCCTTC
GGCGGCGCGCGCGGGCGCACGGACGTGCCGAAGATCGTGGACTGGTACATGGAGGGGAAGATCAACATCGATGATCTCAT
CACCCACACCATGCCACTCGACGAGATCAACACCGCCTTCGACCTGATGCACGAGGGCAAGTCCATCCGCTCGGTGGTGA
TCTACTGA

Upstream 100 bases:

>100_bases
CGGTTTCCCGTAGCGAAGCTGCGGAGTAGAACCACCCGAGCCGCCCCAAGAATCGACGAATCGTGGCGCCCTGCGTCGCG
ATCAACGCCAGAGGTCTGTC

Downstream 100 bases:

>100_bases
TGGAGACCCTCTCCACGGCGAAGTCCCACGGCGGCGTGCAGGGGGTGTACCGGCACGTCTCCGAGGCCACCGGAACCGAG
ATGACCTTCGCCGTCTTCGT

Product: glutathione-dependent formaldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKSRAAVAWEAKKPLTIETIEIGGPKAGEVLVEIMATGVCHTDAYTLDGLDSEGKFPAILGHEGAGIVREVGAGVTTLKV
GDHVIPLYTPECRQCKTCLSQRSNLCTSIRATQGQGLMPDRTSRFSCEPVNGRGGEIFHYMGCSTFSNFTVLPEIALAKV
REDAPFDKICYIGCGVTTGIGAVVYTGKVWPGANVAVFGLGGIGLNVIQGARMVGADKIIGIDINPAKVEMAKKFGMTDF
INPLEVGSDKVVSAIQDLTDGGADFTFDCTGNVTVMRQALEACHRGWGTSIVIGVAPAGAEISTRPFQLVTGRNWRGSAF
GGARGRTDVPKIVDWYMEGKINIDDLITHTMPLDEINTAFDLMHEGKSIRSVVIY

Sequences:

>Translated_375_residues
MKSRAAVAWEAKKPLTIETIEIGGPKAGEVLVEIMATGVCHTDAYTLDGLDSEGKFPAILGHEGAGIVREVGAGVTTLKV
GDHVIPLYTPECRQCKTCLSQRSNLCTSIRATQGQGLMPDRTSRFSCEPVNGRGGEIFHYMGCSTFSNFTVLPEIALAKV
REDAPFDKICYIGCGVTTGIGAVVYTGKVWPGANVAVFGLGGIGLNVIQGARMVGADKIIGIDINPAKVEMAKKFGMTDF
INPLEVGSDKVVSAIQDLTDGGADFTFDCTGNVTVMRQALEACHRGWGTSIVIGVAPAGAEISTRPFQLVTGRNWRGSAF
GGARGRTDVPKIVDWYMEGKINIDDLITHTMPLDEINTAFDLMHEGKSIRSVVIY
>Mature_375_residues
MKSRAAVAWEAKKPLTIETIEIGGPKAGEVLVEIMATGVCHTDAYTLDGLDSEGKFPAILGHEGAGIVREVGAGVTTLKV
GDHVIPLYTPECRQCKTCLSQRSNLCTSIRATQGQGLMPDRTSRFSCEPVNGRGGEIFHYMGCSTFSNFTVLPEIALAKV
REDAPFDKICYIGCGVTTGIGAVVYTGKVWPGANVAVFGLGGIGLNVIQGARMVGADKIIGIDINPAKVEMAKKFGMTDF
INPLEVGSDKVVSAIQDLTDGGADFTFDCTGNVTVMRQALEACHRGWGTSIVIGVAPAGAEISTRPFQLVTGRNWRGSAF
GGARGRTDVPKIVDWYMEGKINIDDLITHTMPLDEINTAFDLMHEGKSIRSVVIY

Specific function: Oxidizes long-chain aliphatic alcohols, long-chain hydroxylated fatty acids and S-hydroxymethylglutathione (hmGSH) in increasing order of preference. Shows little or no activity with short-chain aliphatic alcohols [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=373, Percent_Identity=59.2493297587131, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI71565152, Length=379, Percent_Identity=46.4379947229551, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI34577061, Length=375, Percent_Identity=44.8, Blast_Score=354, Evalue=9e-98,
Organism=Homo sapiens, GI4501929, Length=375, Percent_Identity=44.5333333333333, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI262073058, Length=375, Percent_Identity=44, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI4501933, Length=375, Percent_Identity=44, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI71743840, Length=375, Percent_Identity=44, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI156523966, Length=374, Percent_Identity=42.2459893048128, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI4501939, Length=367, Percent_Identity=42.5068119891008, Blast_Score=327, Evalue=1e-89,
Organism=Escherichia coli, GI1786552, Length=375, Percent_Identity=59.2, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI87082125, Length=388, Percent_Identity=24.2268041237113, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788073, Length=368, Percent_Identity=25, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081918, Length=343, Percent_Identity=30.6122448979592, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787863, Length=367, Percent_Identity=25.6130790190736, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790045, Length=357, Percent_Identity=24.0896358543417, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788075, Length=374, Percent_Identity=25.4010695187166, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI226510992, Length=382, Percent_Identity=24.3455497382199, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786825, Length=396, Percent_Identity=23.989898989899, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786518, Length=380, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=378, Percent_Identity=55.5555555555556, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71997431, Length=380, Percent_Identity=55.2631578947368, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17565904, Length=348, Percent_Identity=53.735632183908, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17562876, Length=362, Percent_Identity=27.9005524861878, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71988145, Length=378, Percent_Identity=25.6613756613757, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562584, Length=365, Percent_Identity=26.027397260274, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17562878, Length=262, Percent_Identity=28.6259541984733, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=380, Percent_Identity=26.0526315789474, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320033, Length=375, Percent_Identity=55.4666666666667, Blast_Score=434, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6323729, Length=377, Percent_Identity=27.3209549071618, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322619, Length=399, Percent_Identity=27.8195488721804, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6319621, Length=380, Percent_Identity=28.9473684210526, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319955, Length=399, Percent_Identity=27.5689223057644, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17737895, Length=374, Percent_Identity=59.6256684491979, Blast_Score=463, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.1; =1.1.1.284 [H]

Molecular weight: Translated: 40007; Mature: 40007

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRAAVAWEAKKPLTIETIEIGGPKAGEVLVEIMATGVCHTDAYTLDGLDSEGKFPAIL
CCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEE
GHEGAGIVREVGAGVTTLKVGDHVIPLYTPECRQCKTCLSQRSNLCTSIRATQGQGLMPD
CCCCCCHHHHHCCCEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCC
RTSRFSCEPVNGRGGEIFHYMGCSTFSNFTVLPEIALAKVREDAPFDKICYIGCGVTTGI
CCCCEEECCCCCCCCEEEEEECCCCCCCCEECHHHHHHHHHCCCCCCCEEEEECCHHHCC
GAVVYTGKVWPGANVAVFGLGGIGLNVIQGARMVGADKIIGIDINPAKVEMAKKFGMTDF
CEEEEECCCCCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHCCCHHC
INPLEVGSDKVVSAIQDLTDGGADFTFDCTGNVTVMRQALEACHRGWGTSIVIGVAPAGA
CCHHHCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
EISTRPFQLVTGRNWRGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHTMPLDEINTAF
CCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHH
DLMHEGKSIRSVVIY
HHHHCCCCCEEEEEC
>Mature Secondary Structure
MKSRAAVAWEAKKPLTIETIEIGGPKAGEVLVEIMATGVCHTDAYTLDGLDSEGKFPAIL
CCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEE
GHEGAGIVREVGAGVTTLKVGDHVIPLYTPECRQCKTCLSQRSNLCTSIRATQGQGLMPD
CCCCCCHHHHHCCCEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCC
RTSRFSCEPVNGRGGEIFHYMGCSTFSNFTVLPEIALAKVREDAPFDKICYIGCGVTTGI
CCCCEEECCCCCCCCEEEEEECCCCCCCCEECHHHHHHHHHCCCCCCCEEEEECCHHHCC
GAVVYTGKVWPGANVAVFGLGGIGLNVIQGARMVGADKIIGIDINPAKVEMAKKFGMTDF
CEEEEECCCCCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHCCCHHC
INPLEVGSDKVVSAIQDLTDGGADFTFDCTGNVTVMRQALEACHRGWGTSIVIGVAPAGA
CCHHHCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
EISTRPFQLVTGRNWRGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHTMPLDEINTAF
CCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHH
DLMHEGKSIRSVVIY
HHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8631716 [H]