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The map label for this gene is glcF [H]

Identifier: 158421704

GI number: 158421704

Start: 97665

End: 98990

Strand: Reverse

Name: glcF [H]

Synonym: AZC_0080

Alternate gene names: 158421704

Gene position: 98990-97665 (Counterclockwise)

Preceding gene: 158421705

Following gene: 158421703

Centisome position: 1.84

GC content: 67.65

Gene sequence:

>1326_bases
ATGAAGACTGAATTCTCCCTCGCGCAGCTTGCCGATCCGCACACCAGCGAGGCGGAAAAGATCCTGCGCACCTGCGTGCA
TTGCGGTTTCTGCACTGCCACGTGTCCCACCTATGTGCTGCTGGGGGACGAGCTCGACAGCCCGCGCGGGCGCATCTACC
TCATCAAGGACATGCTCGAAAATGGCCGGCCCGCGACCGAGGAAGAGGTCAAGCATGTGGACCGCTGCCTCTCCTGCCTC
TCCTGCATGACCACTTGTCCCTCCGGCGTGCATTACATGCACCTTGTGGACCATGCCCGCGCCCATATCGAGCACACCTA
TAAGCGGCCCTTCTTCGACCGGCTGAACCGCGCCCTGCTCGCCGCCGTGATGCCCTATCGCGGCCGGTTCCGCCTCGCGC
TTGCCGGGGCCGCGCTGGCCAAGCCCTTCGCGCCGCTGTTCGATGCCGTGAAGCCGCTGAAGCCTCTCGCCGCCATGCTG
CGCCTCGCGCCAGGCTTTCCGCGGGGCCGCTCGCCCAGTGAAGACCCCGGCGTCCATCCGGCCAAGGGCCCGCGCCGGGC
CCGTGTGGCGCTGCTGGGCGGCTGCGCCCAGCCGGTGCTGCGGCCGGAGACGGACGAGGCCGCCATCCGCCTGCTCAACC
GGCTCGGCGTCGAGGTGGTGCGGGTGGAGAAAGAGGGGTGCTGCGGCTCCCTTGTCCATCACATGGGCAAGGAGGAGCAG
GGCCTTGCGCTGGCCCGCGCCAATGTGGATGCCTGGTGCGCGGAGATGGACAAAAGCGGGCTCGACGCCATCCTCGTCAC
CGTGTCCGGCTGCGGCACCACCATCAAAGACTATGGCCACATGCTTCGCCTTGATCCCGCTTACGCGGAGAAGGCGCGCC
GTGTCTCGGCCATCGCCAGGGACATTTCGGAATATCTCGGCACCCTCGGCCTGCCGAAAGCCGAGAAGCCCATGGGCCGC
AAGATCGCCTATCACGCCGCCTGCTCGCTCCAGCACGGGCAAAAGGTGAAGGGCACGCCGGTGCAGTTGCTGCGCACGGT
CGGCTTCGAGGTGGCCGAGCCCTTCGAGAGTCATATCTGCTGCGGCTCGGCCGGCACCTACAACATGCTCCAGAGCGAGA
TCGCCGTGCAGTTGCGCGACCGGAAGGTCGCCAATCTCGGCAAGCTCGCGCCGGACATCATCGCCGCCGGAAACATCGGC
TGCATGACGCAGATCGGCTCGGGCACCGATACGCCGGTGGTGCACACGGTGGAGTTGCTCGACTGGGCGACTGGCGGTCC
GGTCCCGGCGGCGGTGGCTGCGGCGGGCCTTTCCGCGGCCGCGTGA

Upstream 100 bases:

>100_bases
GGGAGGGGGGTAAGACGGCCGGGCCCGAGAACGCCCGCTCCGAAACGAGCCCCACCCCCATCAGAACAAGAACGCCCGCT
TCCACGGGAGGCCACGCCAG

Downstream 100 bases:

>100_bases
ATAATCCCGGCCGGATTATTGCCGGTACGGCAAAAGTCTCTTGGCCCGCCATCGCGTTTCGTGGCGGACGGCCAAGGTTT
ATCTCGCCTTTCACTTCGCC

Product: glycolate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKTEFSLAQLADPHTSEAEKILRTCVHCGFCTATCPTYVLLGDELDSPRGRIYLIKDMLENGRPATEEEVKHVDRCLSCL
SCMTTCPSGVHYMHLVDHARAHIEHTYKRPFFDRLNRALLAAVMPYRGRFRLALAGAALAKPFAPLFDAVKPLKPLAAML
RLAPGFPRGRSPSEDPGVHPAKGPRRARVALLGGCAQPVLRPETDEAAIRLLNRLGVEVVRVEKEGCCGSLVHHMGKEEQ
GLALARANVDAWCAEMDKSGLDAILVTVSGCGTTIKDYGHMLRLDPAYAEKARRVSAIARDISEYLGTLGLPKAEKPMGR
KIAYHAACSLQHGQKVKGTPVQLLRTVGFEVAEPFESHICCGSAGTYNMLQSEIAVQLRDRKVANLGKLAPDIIAAGNIG
CMTQIGSGTDTPVVHTVELLDWATGGPVPAAVAAAGLSAAA

Sequences:

>Translated_441_residues
MKTEFSLAQLADPHTSEAEKILRTCVHCGFCTATCPTYVLLGDELDSPRGRIYLIKDMLENGRPATEEEVKHVDRCLSCL
SCMTTCPSGVHYMHLVDHARAHIEHTYKRPFFDRLNRALLAAVMPYRGRFRLALAGAALAKPFAPLFDAVKPLKPLAAML
RLAPGFPRGRSPSEDPGVHPAKGPRRARVALLGGCAQPVLRPETDEAAIRLLNRLGVEVVRVEKEGCCGSLVHHMGKEEQ
GLALARANVDAWCAEMDKSGLDAILVTVSGCGTTIKDYGHMLRLDPAYAEKARRVSAIARDISEYLGTLGLPKAEKPMGR
KIAYHAACSLQHGQKVKGTPVQLLRTVGFEVAEPFESHICCGSAGTYNMLQSEIAVQLRDRKVANLGKLAPDIIAAGNIG
CMTQIGSGTDTPVVHTVELLDWATGGPVPAAVAAAGLSAAA
>Mature_441_residues
MKTEFSLAQLADPHTSEAEKILRTCVHCGFCTATCPTYVLLGDELDSPRGRIYLIKDMLENGRPATEEEVKHVDRCLSCL
SCMTTCPSGVHYMHLVDHARAHIEHTYKRPFFDRLNRALLAAVMPYRGRFRLALAGAALAKPFAPLFDAVKPLKPLAAML
RLAPGFPRGRSPSEDPGVHPAKGPRRARVALLGGCAQPVLRPETDEAAIRLLNRLGVEVVRVEKEGCCGSLVHHMGKEEQ
GLALARANVDAWCAEMDKSGLDAILVTVSGCGTTIKDYGHMLRLDPAYAEKARRVSAIARDISEYLGTLGLPKAEKPMGR
KIAYHAACSLQHGQKVKGTPVQLLRTVGFEVAEPFESHICCGSAGTYNMLQSEIAVQLRDRKVANLGKLAPDIIAAGNIG
CMTQIGSGTDTPVVHTVELLDWATGGPVPAAVAAAGLSAAA

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=424, Percent_Identity=43.1603773584906, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1786497, Length=240, Percent_Identity=27.0833333333333, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788576, Length=425, Percent_Identity=25.6470588235294, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 47395; Mature: 47395

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEFSLAQLADPHTSEAEKILRTCVHCGFCTATCPTYVLLGDELDSPRGRIYLIKDMLE
CCCCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCCCEEEEHHHHH
NGRPATEEEVKHVDRCLSCLSCMTTCPSGVHYMHLVDHARAHIEHTYKRPFFDRLNRALL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AAVMPYRGRFRLALAGAALAKPFAPLFDAVKPLKPLAAMLRLAPGFPRGRSPSEDPGVHP
HHHCCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AKGPRRARVALLGGCAQPVLRPETDEAAIRLLNRLGVEVVRVEKEGCCGSLVHHMGKEEQ
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCCC
GLALARANVDAWCAEMDKSGLDAILVTVSGCGTTIKDYGHMLRLDPAYAEKARRVSAIAR
CCEEECCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCEEEECHHHHHHHHHHHHHHH
DISEYLGTLGLPKAEKPMGRKIAYHAACSLQHGQKVKGTPVQLLRTVGFEVAEPFESHIC
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHCCHHHCEE
CGSAGTYNMLQSEIAVQLRDRKVANLGKLAPDIIAAGNIGCMTQIGSGTDTPVVHTVELL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEECCCCCCCCHHHHHHHH
DWATGGPVPAAVAAAGLSAAA
HHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTEFSLAQLADPHTSEAEKILRTCVHCGFCTATCPTYVLLGDELDSPRGRIYLIKDMLE
CCCCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCCCEEEEHHHHH
NGRPATEEEVKHVDRCLSCLSCMTTCPSGVHYMHLVDHARAHIEHTYKRPFFDRLNRALL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AAVMPYRGRFRLALAGAALAKPFAPLFDAVKPLKPLAAMLRLAPGFPRGRSPSEDPGVHP
HHHCCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AKGPRRARVALLGGCAQPVLRPETDEAAIRLLNRLGVEVVRVEKEGCCGSLVHHMGKEEQ
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCCC
GLALARANVDAWCAEMDKSGLDAILVTVSGCGTTIKDYGHMLRLDPAYAEKARRVSAIAR
CCEEECCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCEEEECHHHHHHHHHHHHHHH
DISEYLGTLGLPKAEKPMGRKIAYHAACSLQHGQKVKGTPVQLLRTVGFEVAEPFESHIC
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHCCHHHCEE
CGSAGTYNMLQSEIAVQLRDRKVANLGKLAPDIIAAGNIGCMTQIGSGTDTPVVHTVELL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEECCCCCCCCHHHHHHHH
DWATGGPVPAAVAAAGLSAAA
HHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]