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The map label for this gene is thiE [H]

Identifier: 158421703

GI number: 158421703

Start: 96908

End: 97624

Strand: Reverse

Name: thiE [H]

Synonym: AZC_0079

Alternate gene names: 158421703

Gene position: 97624-96908 (Counterclockwise)

Preceding gene: 158421704

Following gene: 158421702

Centisome position: 1.82

GC content: 69.74

Gene sequence:

>717_bases
TTGGCCCGCCATCGCGTTTCGTGGCGGACGGCCAAGGTTTATCTCGCCTTTCACTTCGCCTCAGGCCGAGGACGTGTCAT
GCCCCATCCGTTCGACCTCTCCCTCTATCTCGTGACCGACCGCCGGCTGACGGCCGAGCGGGGCCTTCTCGAGACGGTCG
AGGAAGCGGTGGCGGGTGGCGTCACGCTCGTGCAGTTGCGCGATCCGGAGGCGAAGGGCCGGGCGCTGGCGGAAGAAGCC
CGCGCCCTCATCGGCCTGCTGCGGCCGAAGGGCATTCCCCTCATCATCAACGACCGGGTGGATGTGGCCGCCGCCGTGGG
CGCCGAGGGCGTGCATCTGGGGCAGGACGACCTTGATCCCGCCGCCGCACGCGCCATCCTCGGGCCGGACGCCATCATCG
GCCTCTCGGTGGGTTCGCTGGAGGAGCTGTCCGCCTCGAACCTCGGCCCGGTGGACTATGTGGGCTGCGGCCCCATCAAC
GCCACCGGCACCAAGGGCGATGCGGGCGGGGCCATCGGCATCGAGGGCTTCGCCTTCCTGCGCTCCCACATCGCGTTGCC
CATGGTCGGCATCGGCGGGCTGAAGGCGGAGGATGCCGAAGCGGTGATGCAGGCGGGCGCGAACGGCATCGCTGTCGTTT
CCGCTCTGTGCGCCGCCCCGGACGTGACCGAGGCCGCCCGCACTCTCAAGACCATCATTGAGACGTCCCGCCGCTGA

Upstream 100 bases:

>100_bases
GCGACTGGCGGTCCGGTCCCGGCGGCGGTGGCTGCGGCGGGCCTTTCCGCGGCCGCGTGAATAATCCCGGCCGGATTATT
GCCGGTACGGCAAAAGTCTC

Downstream 100 bases:

>100_bases
GCGGCAAGTTCTGTTCCTTTCTGCGTTCCAACGACAGTCCCTCAAGGAGACCGACATCATGTCGACCCCCGCCCTTCCCT
CAGGCGTCGCCATCACCGGG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MARHRVSWRTAKVYLAFHFASGRGRVMPHPFDLSLYLVTDRRLTAERGLLETVEEAVAGGVTLVQLRDPEAKGRALAEEA
RALIGLLRPKGIPLIINDRVDVAAAVGAEGVHLGQDDLDPAAARAILGPDAIIGLSVGSLEELSASNLGPVDYVGCGPIN
ATGTKGDAGGAIGIEGFAFLRSHIALPMVGIGGLKAEDAEAVMQAGANGIAVVSALCAAPDVTEAARTLKTIIETSRR

Sequences:

>Translated_238_residues
MARHRVSWRTAKVYLAFHFASGRGRVMPHPFDLSLYLVTDRRLTAERGLLETVEEAVAGGVTLVQLRDPEAKGRALAEEA
RALIGLLRPKGIPLIINDRVDVAAAVGAEGVHLGQDDLDPAAARAILGPDAIIGLSVGSLEELSASNLGPVDYVGCGPIN
ATGTKGDAGGAIGIEGFAFLRSHIALPMVGIGGLKAEDAEAVMQAGANGIAVVSALCAAPDVTEAARTLKTIIETSRR
>Mature_237_residues
ARHRVSWRTAKVYLAFHFASGRGRVMPHPFDLSLYLVTDRRLTAERGLLETVEEAVAGGVTLVQLRDPEAKGRALAEEAR
ALIGLLRPKGIPLIINDRVDVAAAVGAEGVHLGQDDLDPAAARAILGPDAIIGLSVGSLEELSASNLGPVDYVGCGPINA
TGTKGDAGGAIGIEGFAFLRSHIALPMVGIGGLKAEDAEAVMQAGANGIAVVSALCAAPDVTEAARTLKTIIETSRR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=195, Percent_Identity=31.7948717948718, Blast_Score=71, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=231, Percent_Identity=36.3636363636364, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24673; Mature: 24541

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARHRVSWRTAKVYLAFHFASGRGRVMPHPFDLSLYLVTDRRLTAERGLLETVEEAVAGG
CCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCC
VTLVQLRDPEAKGRALAEEARALIGLLRPKGIPLIINDRVDVAAAVGAEGVHLGQDDLDP
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEECCCCCCEECCCCCCCH
AAARAILGPDAIIGLSVGSLEELSASNLGPVDYVGCGPINATGTKGDAGGAIGIEGFAFL
HHHHHHCCCCEEEEECCCCHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHH
RSHIALPMVGIGGLKAEDAEAVMQAGANGIAVVSALCAAPDVTEAARTLKTIIETSRR
HHHHCCCEECCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARHRVSWRTAKVYLAFHFASGRGRVMPHPFDLSLYLVTDRRLTAERGLLETVEEAVAGG
CCCCCCEEEEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCC
VTLVQLRDPEAKGRALAEEARALIGLLRPKGIPLIINDRVDVAAAVGAEGVHLGQDDLDP
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEECCCCCCEECCCCCCCH
AAARAILGPDAIIGLSVGSLEELSASNLGPVDYVGCGPINATGTKGDAGGAIGIEGFAFL
HHHHHHCCCCEEEEECCCCHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHH
RSHIALPMVGIGGLKAEDAEAVMQAGANGIAVVSALCAAPDVTEAARTLKTIIETSRR
HHHHCCCEECCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA