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The map label for this gene is pleD [H]

Identifier: 158338941

GI number: 158338941

Start: 5922590

End: 5923939

Strand: Reverse

Name: pleD [H]

Synonym: AM1_5858

Alternate gene names: 158338941

Gene position: 5923939-5922590 (Counterclockwise)

Preceding gene: 158338942

Following gene: 158338940

Centisome position: 91.09

GC content: 46.3

Gene sequence:

>1350_bases
ATGTCCTTACCGTTTTCCCCACAATCCTCCTTGCACCAACGTCTCAGGGTTGGGCTAGGAACTATGCTGGTGCCCCTGAT
TATGCTGGCGGGGGGGGCCTTGATTTCCTTTGAGGGAGCTGTTGGAAGTTTTGAGCAACAAGATAGTATTAGCCGACAAG
CTTTATTCCCTCTCGCTGAGGTTGAGGGGGAAATTCTTGAGTTAACAAGTGTAGCTACCCATGCTCAACCGACCAATACG
ATGACATTACAGAATTATCGTCGGAAACAGTTGGTGATTAAGTCCCAACTGGCACTACTTTCAGATTATTTTCAAGGGCA
GCCAACGAAGTTTAATTCTGTTCAAGAAATTCGTCGCCAATGGCTGGAGATTGCCAGACTATGCGAGCAAATTTTCGAGG
CCACAGAACCTGAGCAGCTAGCTAATGCCAAAGTTCAGCTTAGTCAGAGGCTACAGGGGATTTTGAAAAACACACAAGAG
TTGAATTATCGGTTGATTGCGTTTCAAAATGAAGCTAACTTTGACAAAGCTAGGGCGGTACGAGAAAAGGTAAGATATTT
GGTCGCTTTCATTTCTTTGTTGGCACTAGCATTAGCCTTATTGTTTGCCTTTTATTTGTCCAGGTCCATTACAGACCCCT
TGCAGACCTTGGCAAAAGGGGTTGAGAAATTAGGCCAGGGGGAGTTGCACCACCGCATTACTCTAGAGACCCAAGATGAA
TTTAACCATTTGGCTGAAACATTTAATCTGATGGCCAATCAGTTAGAGCAGAGCCAGCAGGATTTGTATCGATTAGCCAC
ACTGGATGGTCTTACTGAAGTATACAATCGCCGAGAATTTAATCGGTGGTTGTTGGTGGAAATTGAGCGATCTCAACGGG
GTGAACATCCCGTCTCCCTGATTATGGTGGATATCGATCATTTCAAACGGTTGAATGATACCTATGGTCATCAGGCTGGG
GATCTCGCTCTATGTGAAGTTGCCCAACTTCTTCAGCGGGAGGTCCGACCGGGTGATATTGTCTGTCGTTATGGTGGTGA
AGAGTTTGGTGTAATACTGCCGAAGGCAACGAGTGCGGATAGCTGGGTTATCGCAGAGCGAATTCGATTAGCCATTGCCG
AGAAATCCGTTGTGCTTCCGTCACAGCAGCATATCGATATGACTGCAAGTCTAGGGGTGGCGACCTATCCAGATCACGCT
GATTGCAAAGAGCGGTTAGTTAAGCAGGCGGATCAGGCACTTTATACGGCTAAATATCAAGGGCGCAACCGTGTCCATTC
TGCTGAGGTCAATGAATGCAATCACCAAATCAATAAGCTCCAACGGATGCTTGTTAAACAAGGTACTTAA

Upstream 100 bases:

>100_bases
AAGGTTTTTGGTTTGCTCGTCCCTTAGAGCCTGATGATGCAAAAAAATGGCTTTTAACGTTGAAGAACCATAGAAAATTA
TTGACCTAGCGGAGTATTGC

Downstream 100 bases:

>100_bases
TGTCAGGTTCCACCCATTCTACCTGAGTCTATCCATATGCTCTTAATTCGTTAGCTTTTCGGTGAAAAGCAGTTGGTTAG
TGAGGTTGTAGTTATGCACC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSLPFSPQSSLHQRLRVGLGTMLVPLIMLAGGALISFEGAVGSFEQQDSISRQALFPLAEVEGEILELTSVATHAQPTNT
MTLQNYRRKQLVIKSQLALLSDYFQGQPTKFNSVQEIRRQWLEIARLCEQIFEATEPEQLANAKVQLSQRLQGILKNTQE
LNYRLIAFQNEANFDKARAVREKVRYLVAFISLLALALALLFAFYLSRSITDPLQTLAKGVEKLGQGELHHRITLETQDE
FNHLAETFNLMANQLEQSQQDLYRLATLDGLTEVYNRREFNRWLLVEIERSQRGEHPVSLIMVDIDHFKRLNDTYGHQAG
DLALCEVAQLLQREVRPGDIVCRYGGEEFGVILPKATSADSWVIAERIRLAIAEKSVVLPSQQHIDMTASLGVATYPDHA
DCKERLVKQADQALYTAKYQGRNRVHSAEVNECNHQINKLQRMLVKQGT

Sequences:

>Translated_449_residues
MSLPFSPQSSLHQRLRVGLGTMLVPLIMLAGGALISFEGAVGSFEQQDSISRQALFPLAEVEGEILELTSVATHAQPTNT
MTLQNYRRKQLVIKSQLALLSDYFQGQPTKFNSVQEIRRQWLEIARLCEQIFEATEPEQLANAKVQLSQRLQGILKNTQE
LNYRLIAFQNEANFDKARAVREKVRYLVAFISLLALALALLFAFYLSRSITDPLQTLAKGVEKLGQGELHHRITLETQDE
FNHLAETFNLMANQLEQSQQDLYRLATLDGLTEVYNRREFNRWLLVEIERSQRGEHPVSLIMVDIDHFKRLNDTYGHQAG
DLALCEVAQLLQREVRPGDIVCRYGGEEFGVILPKATSADSWVIAERIRLAIAEKSVVLPSQQHIDMTASLGVATYPDHA
DCKERLVKQADQALYTAKYQGRNRVHSAEVNECNHQINKLQRMLVKQGT
>Mature_448_residues
SLPFSPQSSLHQRLRVGLGTMLVPLIMLAGGALISFEGAVGSFEQQDSISRQALFPLAEVEGEILELTSVATHAQPTNTM
TLQNYRRKQLVIKSQLALLSDYFQGQPTKFNSVQEIRRQWLEIARLCEQIFEATEPEQLANAKVQLSQRLQGILKNTQEL
NYRLIAFQNEANFDKARAVREKVRYLVAFISLLALALALLFAFYLSRSITDPLQTLAKGVEKLGQGELHHRITLETQDEF
NHLAETFNLMANQLEQSQQDLYRLATLDGLTEVYNRREFNRWLLVEIERSQRGEHPVSLIMVDIDHFKRLNDTYGHQAGD
LALCEVAQLLQREVRPGDIVCRYGGEEFGVILPKATSADSWVIAERIRLAIAEKSVVLPSQQHIDMTASLGVATYPDHAD
CKERLVKQADQALYTAKYQGRNRVHSAEVNECNHQINKLQRMLVKQGT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=185, Percent_Identity=41.0810810810811, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=39.873417721519, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=36.7231638418079, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=38.0681818181818, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=35.672514619883, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788381, Length=322, Percent_Identity=27.639751552795, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=33.9181286549708, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=34.7305389221557, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=32.7160493827161, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=28.3333333333333, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788956, Length=240, Percent_Identity=28.75, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=166, Percent_Identity=32.5301204819277, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081974, Length=167, Percent_Identity=31.7365269461078, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=174, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50896; Mature: 50765

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPFSPQSSLHQRLRVGLGTMLVPLIMLAGGALISFEGAVGSFEQQDSISRQALFPLAE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCHHH
VEGEILELTSVATHAQPTNTMTLQNYRRKQLVIKSQLALLSDYFQGQPTKFNSVQEIRRQ
HCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
WLEIARLCEQIFEATEPEQLANAKVQLSQRLQGILKNTQELNYRLIAFQNEANFDKARAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHH
REKVRYLVAFISLLALALALLFAFYLSRSITDPLQTLAKGVEKLGQGELHHRITLETQDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHH
FNHLAETFNLMANQLEQSQQDLYRLATLDGLTEVYNRREFNRWLLVEIERSQRGEHPVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEE
IMVDIDHFKRLNDTYGHQAGDLALCEVAQLLQREVRPGDIVCRYGGEEFGVILPKATSAD
EEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCC
SWVIAERIRLAIAEKSVVLPSQQHIDMTASLGVATYPDHADCKERLVKQADQALYTAKYQ
HHHHHHHHHHHHHHCCEECCCHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GRNRVHSAEVNECNHQINKLQRMLVKQGT
CCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLPFSPQSSLHQRLRVGLGTMLVPLIMLAGGALISFEGAVGSFEQQDSISRQALFPLAE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCHHH
VEGEILELTSVATHAQPTNTMTLQNYRRKQLVIKSQLALLSDYFQGQPTKFNSVQEIRRQ
HCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
WLEIARLCEQIFEATEPEQLANAKVQLSQRLQGILKNTQELNYRLIAFQNEANFDKARAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHH
REKVRYLVAFISLLALALALLFAFYLSRSITDPLQTLAKGVEKLGQGELHHRITLETQDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHH
FNHLAETFNLMANQLEQSQQDLYRLATLDGLTEVYNRREFNRWLLVEIERSQRGEHPVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEE
IMVDIDHFKRLNDTYGHQAGDLALCEVAQLLQREVRPGDIVCRYGGEEFGVILPKATSAD
EEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCC
SWVIAERIRLAIAEKSVVLPSQQHIDMTASLGVATYPDHADCKERLVKQADQALYTAKYQ
HHHHHHHHHHHHHHCCEECCCHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GRNRVHSAEVNECNHQINKLQRMLVKQGT
CCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA