Click here to switch to the map view.

The map label for this gene is hemA

Identifier: 158338846

GI number: 158338846

Start: 5838559

End: 5839860

Strand: Direct

Name: hemA

Synonym: AM1_5760

Alternate gene names: 158338846

Gene position: 5838559-5839860 (Clockwise)

Preceding gene: 158338845

Following gene: 158338848

Centisome position: 89.77

GC content: 46.39

Gene sequence:

>1302_bases
ATGAATATTGCGGTTGTCGGTTTAAGTCATAAAACAGCGCCAGTGGATGTGCGAGAAAAACTCAGCATTCCCGATGATGT
GAAAGAAAAGGCTACCTCTCAATTAAGGAGTTATCCTCATTTAGAAGAAGTGGCAATTTTGAGTACCTGTAACCGCATGG
AAGTCTATGTGGTTGCGCAAGAAACCGATGACGGTATTCGTGAGCTGACGCAGTTCTTATCTGAGTGGAGTCAAATCCCT
CTGTTAGAGCTTCGTCAGCATTTGTTCATCCTTCTGCATCAAGATGCCGTCATGCATTTGATGCGGGTGTCTGCTGGACT
AGATAGCTTGGTGTTGGGAGAAGGTCAAATCCTGGCCCAAGTGAAACAGACTCACAAATTGAGCCAAAAATACAGCGGCG
CAGGCCCCATTCTCAATCGCTTATTTAAGCAGGCCATCTCTGCAGGTAAGCGAGTTCGAACCGAGACCAGTATCGGTACC
GGAGCAGTATCGATCAGTTCAGCAGCAGTCGAACTTGCCCAGATTAAGACTGAAGATTTATCAGCCCATCGGGTCGCCAT
TATTGGAGCGGGCAAAATGTCTCGCTTGTTGGTCAAACATCTGCTGAGTAAAGGCGCTAATCAAATTGCCATTTTAAATC
GGTCGTTGAAGCGAGCTGAGCAATTGGCCGATCAATTTCAAGGTGCTGATCTAAAACTGCATACTTTAGCCGATATGCAG
GCAGTTCTAACCGAATCTGATTTAATTTTTACGAGTACGGCTTCAACCGAGCCTCTGTTGGATCGCGACATTCTAGAGCC
CATCGTCTGCGATCGCCAATCTTGCATGATCTTTGATATCTCTGTACCTCGAAACGTTCACAGCAATGTGAATGAATTAT
CCCAAGTGCACGCCTTTAATGTGGATGACTTGAAGGCAGTGGTGGCTCAGAACCAAGAGAGCCGACGACAAATGGCACTA
GAGGCTGAGTCCCTGTTAGAAGAAGAAGTTGCCAGTTTTGATGTCTGGTGGCGTTCCCTAGAGACAGTTCCCACCATCAG
TAGTTTAAGAACGAAGGTCGAATCCATTCGAGAGCAGGAATTAGAAAAAGCGCTTTCTCGTCTAGGAACTGAGTTTGCCG
AGAAACATCAAGAAGTGATTGAAGCGTTAACCCGAGGCATCGTCAACAAGATTTTGCACGACCCCATGGTTCAACTGCGG
GCTCAACAAGATATTGAAGTCCGTCGCAAGGCCATGCAATCTCTCAATATGCTCTTTAATCTCAATTCTTCTCAAGGGGT
TGCCAAACAGCGCAATACTTAG

Upstream 100 bases:

>100_bases
AACGTCCTAAGGCACTTCAGTTACATTAGGCGATAGCCTCTTAATTCTGAAGTTTGAATCCCTCAAACGACGCGGCTAGA
TAAACAGAGAAGGAGGACAC

Downstream 100 bases:

>100_bases
GCTTCACGCTGGAACTTGTGCATCAATAACTGTTGACTAACCAATAAGATAAACAGGGGGTTGTTGATTAGATAACGACG
CCAAAGTCTTCGGGGCTCAA

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNIAVVGLSHKTAPVDVREKLSIPDDVKEKATSQLRSYPHLEEVAILSTCNRMEVYVVAQETDDGIRELTQFLSEWSQIP
LLELRQHLFILLHQDAVMHLMRVSAGLDSLVLGEGQILAQVKQTHKLSQKYSGAGPILNRLFKQAISAGKRVRTETSIGT
GAVSISSAAVELAQIKTEDLSAHRVAIIGAGKMSRLLVKHLLSKGANQIAILNRSLKRAEQLADQFQGADLKLHTLADMQ
AVLTESDLIFTSTASTEPLLDRDILEPIVCDRQSCMIFDISVPRNVHSNVNELSQVHAFNVDDLKAVVAQNQESRRQMAL
EAESLLEEEVASFDVWWRSLETVPTISSLRTKVESIREQELEKALSRLGTEFAEKHQEVIEALTRGIVNKILHDPMVQLR
AQQDIEVRRKAMQSLNMLFNLNSSQGVAKQRNT

Sequences:

>Translated_433_residues
MNIAVVGLSHKTAPVDVREKLSIPDDVKEKATSQLRSYPHLEEVAILSTCNRMEVYVVAQETDDGIRELTQFLSEWSQIP
LLELRQHLFILLHQDAVMHLMRVSAGLDSLVLGEGQILAQVKQTHKLSQKYSGAGPILNRLFKQAISAGKRVRTETSIGT
GAVSISSAAVELAQIKTEDLSAHRVAIIGAGKMSRLLVKHLLSKGANQIAILNRSLKRAEQLADQFQGADLKLHTLADMQ
AVLTESDLIFTSTASTEPLLDRDILEPIVCDRQSCMIFDISVPRNVHSNVNELSQVHAFNVDDLKAVVAQNQESRRQMAL
EAESLLEEEVASFDVWWRSLETVPTISSLRTKVESIREQELEKALSRLGTEFAEKHQEVIEALTRGIVNKILHDPMVQLR
AQQDIEVRRKAMQSLNMLFNLNSSQGVAKQRNT
>Mature_433_residues
MNIAVVGLSHKTAPVDVREKLSIPDDVKEKATSQLRSYPHLEEVAILSTCNRMEVYVVAQETDDGIRELTQFLSEWSQIP
LLELRQHLFILLHQDAVMHLMRVSAGLDSLVLGEGQILAQVKQTHKLSQKYSGAGPILNRLFKQAISAGKRVRTETSIGT
GAVSISSAAVELAQIKTEDLSAHRVAIIGAGKMSRLLVKHLLSKGANQIAILNRSLKRAEQLADQFQGADLKLHTLADMQ
AVLTESDLIFTSTASTEPLLDRDILEPIVCDRQSCMIFDISVPRNVHSNVNELSQVHAFNVDDLKAVVAQNQESRRQMAL
EAESLLEEEVASFDVWWRSLETVPTISSLRTKVESIREQELEKALSRLGTEFAEKHQEVIEALTRGIVNKILHDPMVQLR
AQQDIEVRRKAMQSLNMLFNLNSSQGVAKQRNT

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=402, Percent_Identity=36.81592039801, Blast_Score=242, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_ACAM1 (B0BZF9)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001520023.1
- ProteinModelPortal:   B0BZF9
- SMR:   B0BZF9
- GeneID:   5684546
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5760
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- ProtClustDB:   PRK00045
- BioCyc:   AMAR329726:AM1_5760-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR003462
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR13812
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 48282; Mature: 48282

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAVVGLSHKTAPVDVREKLSIPDDVKEKATSQLRSYPHLEEVAILSTCNRMEVYVVAQ
CCEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEE
ETDDGIRELTQFLSEWSQIPLLELRQHLFILLHQDAVMHLMRVSAGLDSLVLGEGQILAQ
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHH
VKQTHKLSQKYSGAGPILNRLFKQAISAGKRVRTETSIGTGAVSISSAAVELAQIKTEDL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCEEHHHHHHHHHHHHHCCC
SAHRVAIIGAGKMSRLLVKHLLSKGANQIAILNRSLKRAEQLADQFQGADLKLHTLADMQ
CCCEEEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
AVLTESDLIFTSTASTEPLLDRDILEPIVCDRQSCMIFDISVPRNVHSNVNELSQVHAFN
HHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHCCHHHHHHHHCCC
VDDLKAVVAQNQESRRQMALEAESLLEEEVASFDVWWRSLETVPTISSLRTKVESIREQE
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LEKALSRLGTEFAEKHQEVIEALTRGIVNKILHDPMVQLRAQQDIEVRRKAMQSLNMLFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHC
LNSSQGVAKQRNT
CCCCCCCCCCCCC
>Mature Secondary Structure
MNIAVVGLSHKTAPVDVREKLSIPDDVKEKATSQLRSYPHLEEVAILSTCNRMEVYVVAQ
CCEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEE
ETDDGIRELTQFLSEWSQIPLLELRQHLFILLHQDAVMHLMRVSAGLDSLVLGEGQILAQ
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHH
VKQTHKLSQKYSGAGPILNRLFKQAISAGKRVRTETSIGTGAVSISSAAVELAQIKTEDL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCEEHHHHHHHHHHHHHCCC
SAHRVAIIGAGKMSRLLVKHLLSKGANQIAILNRSLKRAEQLADQFQGADLKLHTLADMQ
CCCEEEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
AVLTESDLIFTSTASTEPLLDRDILEPIVCDRQSCMIFDISVPRNVHSNVNELSQVHAFN
HHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHCCHHHHHHHHCCC
VDDLKAVVAQNQESRRQMALEAESLLEEEVASFDVWWRSLETVPTISSLRTKVESIREQE
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LEKALSRLGTEFAEKHQEVIEALTRGIVNKILHDPMVQLRAQQDIEVRRKAMQSLNMLFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHC
LNSSQGVAKQRNT
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA