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The map label for this gene is wcaL [H]

Identifier: 158338804

GI number: 158338804

Start: 5793186

End: 5794415

Strand: Reverse

Name: wcaL [H]

Synonym: AM1_5715

Alternate gene names: 158338804

Gene position: 5794415-5793186 (Counterclockwise)

Preceding gene: 158338805

Following gene: 158338803

Centisome position: 89.09

GC content: 42.11

Gene sequence:

>1230_bases
ATGAGAATAGCTTTTATTGTTGGACAATTTCCCGTCGCTTCCAAGACGTTTATCTTAAATCAGATTATTGGTTTAGTAGA
TAGGGGGCATGAAGTGGATATCTATGCGGATGTCCCTGCGTATCAGTCTTCTGTGCATGGTGATATTCGAAATTATGACT
TATATAAAAATATCTGTTATGAATATCCACTGCCTTCTGAGTTCTTAGCCCGACAGGCCGAAAAAATAAAACTACTTGCC
CATTGTTTCTTTAGGAATCCCTTCGCTGTTCTCAATACTCTCAATGCAGCTAGATATGGCCAACAAGCAACCTCCTTCAA
GTTGCTCTGTCGGGCTGAACCCGCTATCTCACGGCCCTCCTCCTATGACATTGTCCATTGCCATTTTGGTTTAAATGCGA
TTCGCGGTTTGCTTCTTAAAGAAGTCAAGGCTTTAAAAGGGAAATTGGTAACCACCTTCCATGGCGTGGACATTACAAAA
CATCTCCAGGTTTATGGCAATGATATGTACGATCAGCTTTGGACAAAAGGTGATCTTTTTTTACCCATTAGTGATCTATT
TCTCAAAAAGATAGTCAACTTAGGATGTCAACAAGACAAGACCCATGTCCACCATATGGGGATTGATTGCAGCAAATTTA
AGTATGTCAGCAGGCATTTGAGTTCAGATCGGCCTATTGTTCTGGCCACTGTCAATCGCCTAGTCGAGAAAAAAGGGGTT
GAGTATTCGATTCGGGCAGTTGCCCAGCTTATCCATAAGAATATTCTTGTCCAGTACAACATTGTTGGTGAGGGACCACT
GTCTGATCATCTTCAACAACTTGTAAAAAACTTGAATATGGAGCGTCACATCCATTTTCTGGGTTGGAAAGAGCAAAGTG
ATGTGATCGAAATTCTTGAGAAGTCAGATATTCTCATCGCTCCGAGTGTCACCAGTAACAATGGTGATCAGGAAGGGATT
CCGGTCGCTTTAATGGAAGCGATGGCTATGGGAATGCCCATTGTCAGTACCCAACATAGCGGTATTCCTGAATTGGTTCA
GCATAATAGGTCTGGTTTCTTGGTGCCAGAACGAGACGTTGATGAACTGGCAAATAAATTAGAGTGTTTGGCGATCAACC
CAAACATGTGGTCCGAGATGGGTTTGACAGGCCGAAGAATTGTCGAGAAAGACTACAACATCCATCACCTGAATGATCAG
TTAGTTGATATTTATCAATCCCTTCTATGA

Upstream 100 bases:

>100_bases
ATTTCTAGGGTTGCGTCATAACACCGTTGTTCGAGAGTCCTATATTTATCCTCGCTATATACAGCCTTATTGCATGAGAT
TATTTTTTGCACTTTAGCTT

Downstream 100 bases:

>100_bases
CGTTAATGAGCAATCGATAGAATTTTTTAATTATGGATTTTGTTTCTGTTATTATCCCGGTTTTTAATGATACTCAGCGT
TTGAGAGAATGCTTAACCGC

Product: glycosyl transferase, group 1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MRIAFIVGQFPVASKTFILNQIIGLVDRGHEVDIYADVPAYQSSVHGDIRNYDLYKNICYEYPLPSEFLARQAEKIKLLA
HCFFRNPFAVLNTLNAARYGQQATSFKLLCRAEPAISRPSSYDIVHCHFGLNAIRGLLLKEVKALKGKLVTTFHGVDITK
HLQVYGNDMYDQLWTKGDLFLPISDLFLKKIVNLGCQQDKTHVHHMGIDCSKFKYVSRHLSSDRPIVLATVNRLVEKKGV
EYSIRAVAQLIHKNILVQYNIVGEGPLSDHLQQLVKNLNMERHIHFLGWKEQSDVIEILEKSDILIAPSVTSNNGDQEGI
PVALMEAMAMGMPIVSTQHSGIPELVQHNRSGFLVPERDVDELANKLECLAINPNMWSEMGLTGRRIVEKDYNIHHLNDQ
LVDIYQSLL

Sequences:

>Translated_409_residues
MRIAFIVGQFPVASKTFILNQIIGLVDRGHEVDIYADVPAYQSSVHGDIRNYDLYKNICYEYPLPSEFLARQAEKIKLLA
HCFFRNPFAVLNTLNAARYGQQATSFKLLCRAEPAISRPSSYDIVHCHFGLNAIRGLLLKEVKALKGKLVTTFHGVDITK
HLQVYGNDMYDQLWTKGDLFLPISDLFLKKIVNLGCQQDKTHVHHMGIDCSKFKYVSRHLSSDRPIVLATVNRLVEKKGV
EYSIRAVAQLIHKNILVQYNIVGEGPLSDHLQQLVKNLNMERHIHFLGWKEQSDVIEILEKSDILIAPSVTSNNGDQEGI
PVALMEAMAMGMPIVSTQHSGIPELVQHNRSGFLVPERDVDELANKLECLAINPNMWSEMGLTGRRIVEKDYNIHHLNDQ
LVDIYQSLL
>Mature_409_residues
MRIAFIVGQFPVASKTFILNQIIGLVDRGHEVDIYADVPAYQSSVHGDIRNYDLYKNICYEYPLPSEFLARQAEKIKLLA
HCFFRNPFAVLNTLNAARYGQQATSFKLLCRAEPAISRPSSYDIVHCHFGLNAIRGLLLKEVKALKGKLVTTFHGVDITK
HLQVYGNDMYDQLWTKGDLFLPISDLFLKKIVNLGCQQDKTHVHHMGIDCSKFKYVSRHLSSDRPIVLATVNRLVEKKGV
EYSIRAVAQLIHKNILVQYNIVGEGPLSDHLQQLVKNLNMERHIHFLGWKEQSDVIEILEKSDILIAPSVTSNNGDQEGI
PVALMEAMAMGMPIVSTQHSGIPELVQHNRSGFLVPERDVDELANKLECLAINPNMWSEMGLTGRRIVEKDYNIHHLNDQ
LVDIYQSLL

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=410, Percent_Identity=33.9024390243902, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 46348; Mature: 46348

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAFIVGQFPVASKTFILNQIIGLVDRGHEVDIYADVPAYQSSVHGDIRNYDLYKNICY
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCHHHHHHHHH
EYPLPSEFLARQAEKIKLLAHCFFRNPFAVLNTLNAARYGQQATSFKLLCRAEPAISRPS
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
SYDIVHCHFGLNAIRGLLLKEVKALKGKLVTTFHGVDITKHLQVYGNDMYDQLWTKGDLF
CCCEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCHHHHHHHCCCCEE
LPISDLFLKKIVNLGCQQDKTHVHHMGIDCSKFKYVSRHLSSDRPIVLATVNRLVEKKGV
ECHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCC
EYSIRAVAQLIHKNILVQYNIVGEGPLSDHLQQLVKNLNMERHIHFLGWKEQSDVIEILE
CHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHH
KSDILIAPSVTSNNGDQEGIPVALMEAMAMGMPIVSTQHSGIPELVQHNRSGFLVPERDV
CCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCCCCCCCCH
DELANKLECLAINPNMWSEMGLTGRRIVEKDYNIHHLNDQLVDIYQSLL
HHHHCCCEEEEECCCCHHHCCCCCHHHHHCCCCEEECCHHHHHHHHHHC
>Mature Secondary Structure
MRIAFIVGQFPVASKTFILNQIIGLVDRGHEVDIYADVPAYQSSVHGDIRNYDLYKNICY
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCHHHHHHHHH
EYPLPSEFLARQAEKIKLLAHCFFRNPFAVLNTLNAARYGQQATSFKLLCRAEPAISRPS
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
SYDIVHCHFGLNAIRGLLLKEVKALKGKLVTTFHGVDITKHLQVYGNDMYDQLWTKGDLF
CCCEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCHHHHHHHCCCCEE
LPISDLFLKKIVNLGCQQDKTHVHHMGIDCSKFKYVSRHLSSDRPIVLATVNRLVEKKGV
ECHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCC
EYSIRAVAQLIHKNILVQYNIVGEGPLSDHLQQLVKNLNMERHIHFLGWKEQSDVIEILE
CHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHH
KSDILIAPSVTSNNGDQEGIPVALMEAMAMGMPIVSTQHSGIPELVQHNRSGFLVPERDV
CCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCCCCCCCCH
DELANKLECLAINPNMWSEMGLTGRRIVEKDYNIHHLNDQLVDIYQSLL
HHHHCCCEEEEECCCCHHHCCCCCHHHHHCCCCEEECCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]