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The map label for this gene is ykuT [H]

Identifier: 158338741

GI number: 158338741

Start: 5718222

End: 5719304

Strand: Direct

Name: ykuT [H]

Synonym: AM1_5651

Alternate gene names: 158338741

Gene position: 5718222-5719304 (Clockwise)

Preceding gene: 158338740

Following gene: 158338742

Centisome position: 87.92

GC content: 44.88

Gene sequence:

>1083_bases
TTGATTGCAGGTGGGCTCACCGTCGGATTGCTGGTATTGACGATTGGCATTAAGTATCGACAGCGACAACTTAAAGCTCG
ATACAAACAGATTTTGGCTGAGATTGAGAAGGCTGAGTCATTCGTAATTTCTTCTTCTGAATCAGATACCAATTCTAAGG
AAACGTTATCTGAGCAGAGCAACGGGGTGGCCCAAAAGCAGCTTGCCAAACACAAAAGATTGAAAGTATGGATGGAACTT
CGACAAAGATTATTGACGATTGGTCTGATTGGAATTTGGGCCGGAGGAGTGGCGTTAGTCTTGCGCCTTTTCCCCCAAAC
CCGGTGGCTGCAGTCATTGGTGTTGTCTGGTTTAGGGCAGGTACTCCTAAAAATTATTGTCATCCTAGTGGCGACCTATT
GGGCGGTTCGGATCAGTTTTGTGTTGATCGATGGGATCTTCACTTTCTTGGGTCGAAGTAGTCTCCTCTCTTCTCCAGCC
TCAGTGCGAAAACAGCAGCGGCTGCAAACGGTCGCAGGCATTGCTAAAGGAATTGTCACCATTTTGATGGGAATTATTGG
ATTCCTGATTGCGCTATCAACCCTGGGAGTCAATATTGGTCCTTTGCTAACAGGAGTAGGGATTATAGGTGTGGCGATAT
CCTTGGGTGCTCAGAATTTAGTGAAGGATATGATTAATGGATTTTTTATCCTGCTGGAAGATCAGTTTAGTGTGGGGGAT
GTGATTACCATCGGGGATGCGTCGGGATTTGTCGAAAAAATGAATTTGCGCATTACTCAATTGCGCAGTATTGACGGTGA
ATTAATTACGGTTCCAAACAGCAGTATTTCAGAAGTCAGAAATCATACGAATAAATGGTCTCGGGCCAACTTAGCGATCG
CAGTTGGGTATCACACTGATCTGGATCAGGCGATTTCGATTATTGATGATGTTGCTCAAGAGATGGGTAGTCCTAGTCCT
GTAAGGATGCATTGTAGTGATGGACAAACATCCAGATTGCTCCAATATGATTCTCTAGCTTTTTCGAAAACGACAGTGTC
TTTCGAACTAACCGAGACACTCGCTGGCGCATGGTGCAATTAA

Upstream 100 bases:

>100_bases
AGGACTAGAAACAGCGACTCAGGGAATCATTCAATCCATTAAGGATGGGCTTCAAAACTATAAGCGGGAACGTCAACCCG
ATTACCTAAGACGGCAAGGG

Downstream 100 bases:

>100_bases
ATCGTTCAATGTAGCAGGTTTGGCCTGTTTCCTTGCCCACAGCTTGATGTCGTTGTTTGGGGAAAACACAGCCGTAGGCA
TCCCAAAAATCCGTGTAGGC

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MIAGGLTVGLLVLTIGIKYRQRQLKARYKQILAEIEKAESFVISSSESDTNSKETLSEQSNGVAQKQLAKHKRLKVWMEL
RQRLLTIGLIGIWAGGVALVLRLFPQTRWLQSLVLSGLGQVLLKIIVILVATYWAVRISFVLIDGIFTFLGRSSLLSSPA
SVRKQQRLQTVAGIAKGIVTILMGIIGFLIALSTLGVNIGPLLTGVGIIGVAISLGAQNLVKDMINGFFILLEDQFSVGD
VITIGDASGFVEKMNLRITQLRSIDGELITVPNSSISEVRNHTNKWSRANLAIAVGYHTDLDQAISIIDDVAQEMGSPSP
VRMHCSDGQTSRLLQYDSLAFSKTTVSFELTETLAGAWCN

Sequences:

>Translated_360_residues
MIAGGLTVGLLVLTIGIKYRQRQLKARYKQILAEIEKAESFVISSSESDTNSKETLSEQSNGVAQKQLAKHKRLKVWMEL
RQRLLTIGLIGIWAGGVALVLRLFPQTRWLQSLVLSGLGQVLLKIIVILVATYWAVRISFVLIDGIFTFLGRSSLLSSPA
SVRKQQRLQTVAGIAKGIVTILMGIIGFLIALSTLGVNIGPLLTGVGIIGVAISLGAQNLVKDMINGFFILLEDQFSVGD
VITIGDASGFVEKMNLRITQLRSIDGELITVPNSSISEVRNHTNKWSRANLAIAVGYHTDLDQAISIIDDVAQEMGSPSP
VRMHCSDGQTSRLLQYDSLAFSKTTVSFELTETLAGAWCN
>Mature_360_residues
MIAGGLTVGLLVLTIGIKYRQRQLKARYKQILAEIEKAESFVISSSESDTNSKETLSEQSNGVAQKQLAKHKRLKVWMEL
RQRLLTIGLIGIWAGGVALVLRLFPQTRWLQSLVLSGLGQVLLKIIVILVATYWAVRISFVLIDGIFTFLGRSSLLSSPA
SVRKQQRLQTVAGIAKGIVTILMGIIGFLIALSTLGVNIGPLLTGVGIIGVAISLGAQNLVKDMINGFFILLEDQFSVGD
VITIGDASGFVEKMNLRITQLRSIDGELITVPNSSISEVRNHTNKWSRANLAIAVGYHTDLDQAISIIDDVAQEMGSPSP
VRMHCSDGQTSRLLQYDSLAFSKTTVSFELTETLAGAWCN

Specific function: May play a role in resistance to osmotic downshock [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI87081787, Length=266, Percent_Identity=31.5789473684211, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI2367355, Length=114, Percent_Identity=34.2105263157895, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786670, Length=165, Percent_Identity=28.4848484848485, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 39151; Mature: 39151

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGGLTVGLLVLTIGIKYRQRQLKARYKQILAEIEKAESFVISSSESDTNSKETLSEQS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHH
NGVAQKQLAKHKRLKVWMELRQRLLTIGLIGIWAGGVALVLRLFPQTRWLQSLVLSGLGQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VLLKIIVILVATYWAVRISFVLIDGIFTFLGRSSLLSSPASVRKQQRLQTVAGIAKGIVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ILMGIIGFLIALSTLGVNIGPLLTGVGIIGVAISLGAQNLVKDMINGFFILLEDQFSVGD
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
VITIGDASGFVEKMNLRITQLRSIDGELITVPNSSISEVRNHTNKWSRANLAIAVGYHTD
EEEECCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCC
LDQAISIIDDVAQEMGSPSPVRMHCSDGQTSRLLQYDSLAFSKTTVSFELTETLAGAWCN
HHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHEEEEEEEEEHHHHHHCCCCC
>Mature Secondary Structure
MIAGGLTVGLLVLTIGIKYRQRQLKARYKQILAEIEKAESFVISSSESDTNSKETLSEQS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHH
NGVAQKQLAKHKRLKVWMELRQRLLTIGLIGIWAGGVALVLRLFPQTRWLQSLVLSGLGQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VLLKIIVILVATYWAVRISFVLIDGIFTFLGRSSLLSSPASVRKQQRLQTVAGIAKGIVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ILMGIIGFLIALSTLGVNIGPLLTGVGIIGVAISLGAQNLVKDMINGFFILLEDQFSVGD
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
VITIGDASGFVEKMNLRITQLRSIDGELITVPNSSISEVRNHTNKWSRANLAIAVGYHTD
EEEECCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCC
LDQAISIIDDVAQEMGSPSPVRMHCSDGQTSRLLQYDSLAFSKTTVSFELTETLAGAWCN
HHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHEEEEEEEEEHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]