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The map label for this gene is uvrB

Identifier: 158338625

GI number: 158338625

Start: 5577955

End: 5579961

Strand: Reverse

Name: uvrB

Synonym: AM1_5529

Alternate gene names: 158338625

Gene position: 5579961-5577955 (Counterclockwise)

Preceding gene: 158338627

Following gene: 158338624

Centisome position: 85.8

GC content: 48.63

Gene sequence:

>2007_bases
ATGACAGCTTTTGACCTTCATGCTCCGTTTGTTCCTAAAGGTGATCAACCCCAAGCTATTGAGAAACTAACAACGCACCT
TCAGTCTGGCCATTCTAGGCAAACGTTGCTGGGTGCGACGGGGACGGGTAAAACCTTCACCATGGCAAAAGTGATTGAAG
AGATTGGTAAACCCACGCTAATCTTGGCCCATAACAAAACCCTAGCTGCACAGCTATGCAACGAAATTCGTTCGTTTTTT
CCCAACAATGCCGTTGAGTATTTCATCAGCTATTACGACTATTACCAACCTGAAGCCTATATTCCGGTCACGGATACCTT
TATTGAAAAAACAGCTTCTATCAATGAAGAAATTGATATGCTGCGGCATTCGGCCACACGGTCACTCTTTGAACGTCAGG
ATGTCATCGTCGTGGCGTCGATCAGCTGTATTTACGGCTTGGGGATTCCCGCTGAGTACCTTAAGGCATCAATCCCCCTT
AAGGTTGGAGCCGAGGTCGATCAGCGGCAGTTGCTACGAAATCTGGCAGCCGTGCAATATACCCGTAATGATGTGGACTT
AGGGCGAGGGCGATTTCGTGTCAAAGGTGATGTTCTGGAAATTGGTCCAGCCTATGAAGACCGGATTATTCGGGTAGAGT
TTTTTGGGGATGAGATCGATGCCATTCGCTATGTCGATCCCGTTACTGGAGAGACATTACAGAGTTTGGAAGGACTGAAT
GTATACCCTGCACGCCACTTCGTAACTCCTACAGAGCGATTGGAGATGGCCGTTGAAGCCATTGACGCGGAGAGAACAGC
CCAAGTTGAGCACCTAGAAGGAGCTGGGAAACTGTTGGAAGCTCAGCGATTGAATCAGCGCACTCGATACGATTTGGAAA
TGTTACGAGAGGTGGGCTATTGCAACGGCGTGGAGAATTATTCTCGCCATCTGGCGGGTCGGCAGGCGGGAGAGCCGCCA
GAATGCCTGGTAGATTATTTCCCCAAGGACTGGTTATTAATTGTGGATGAGTCCCATGTGACCGTACCTCAGATTCGAGG
CATGTATAACGGTGATCAGGCCCGTAAAAAGGTTTTGATTGATCACGGTTTTCGGCTCCCCAGTGCAGCAGATAATCGTC
CTCTCAAGTCTGAGGAATTTTGGCAGAAAGTGGGGCAGTGTGTATTTGTATCAGCGACTCCCGGCCTTTGGGAAATCGAG
TTATCAGAGAATCGCGTGGTTGAACAAGTGATTCGACCCACTGGCGTTGTCGATCCAGAAATTTTTGTGCGTCCAACAAC
AGGCCAAGTGGACGATTTATTGGCTGAAATTCAAGAGCGGGTGGGGCGACAAGAACGGACGTTAATTACGACTCTAACTA
AACGGATGGCGGAGGACCTGACGGAGTACTTTGAAGAGCGGGGGGTACGCGTTCGCTATTTGCACTCAGAAATTAATGCC
ATTCAACGCATTGAAATTTTAAGGGATCTACGGGAGGGCGTATTTGATGTGCTCATTGGAGTCAATTTATTGCGAGAGGG
ATTGGACTTACCGGAAGTTTCTCTCGTGGCGATCTTGGATGCAGACAAGGAAGGGTTTCTCAGAGCTGAACGCTCATTGA
TTCAAACCATTGGCCGGGCTGCACGACATGTCCGGGGCCAAGCGATTCTCTATGCCGATAATCTAACGGATAGTATGGAG
AAGGCGATTTCCGAAACTGAACGGCGGCGTAAGATTCAGCTTGCCTACAATAAAAAACATGGCATCACTCCACAACCGGT
CAAAAAAGGATCTGACAATGCGATTTTGGCCTTTCTGGAAGTCTCCCGCCGCCTCAATACCCAGGAACTTGAAGATGCTT
ATGAACAAGCGGATGATTTGCCCTTGGAAAGCGTGCCAGAGTTGATCACCCAGTTGGAAGCACAGATGAAAGAAGCTGCG
AAGAATCTGGAGTTTGAGGAGGCCGCAAAATATCGCGATCGCATCAAGAATCTAAGATCTAAACTCCTCGGTCAACAGTC
CCCTTGA

Upstream 100 bases:

>100_bases
GAACGTGACAGTTGTCACGGGGGGGTATTGTTCTATGTCATGCTGCTGCCGTAGTTCGAAAAATTCGGCATTATAAAGAG
GCAGTCACCTCTTCGCCCCA

Downstream 100 bases:

>100_bases
TCAGATTGCCACTTCTTTGGGAAATATCACCTTAGGCCAGGTCTAAGTCCATCTTTTTTTATCCAGTTCCGATCTGCAAG
GTACGCTGGCAATTCATGAA

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MTAFDLHAPFVPKGDQPQAIEKLTTHLQSGHSRQTLLGATGTGKTFTMAKVIEEIGKPTLILAHNKTLAAQLCNEIRSFF
PNNAVEYFISYYDYYQPEAYIPVTDTFIEKTASINEEIDMLRHSATRSLFERQDVIVVASISCIYGLGIPAEYLKASIPL
KVGAEVDQRQLLRNLAAVQYTRNDVDLGRGRFRVKGDVLEIGPAYEDRIIRVEFFGDEIDAIRYVDPVTGETLQSLEGLN
VYPARHFVTPTERLEMAVEAIDAERTAQVEHLEGAGKLLEAQRLNQRTRYDLEMLREVGYCNGVENYSRHLAGRQAGEPP
ECLVDYFPKDWLLIVDESHVTVPQIRGMYNGDQARKKVLIDHGFRLPSAADNRPLKSEEFWQKVGQCVFVSATPGLWEIE
LSENRVVEQVIRPTGVVDPEIFVRPTTGQVDDLLAEIQERVGRQERTLITTLTKRMAEDLTEYFEERGVRVRYLHSEINA
IQRIEILRDLREGVFDVLIGVNLLREGLDLPEVSLVAILDADKEGFLRAERSLIQTIGRAARHVRGQAILYADNLTDSME
KAISETERRRKIQLAYNKKHGITPQPVKKGSDNAILAFLEVSRRLNTQELEDAYEQADDLPLESVPELITQLEAQMKEAA
KNLEFEEAAKYRDRIKNLRSKLLGQQSP

Sequences:

>Translated_668_residues
MTAFDLHAPFVPKGDQPQAIEKLTTHLQSGHSRQTLLGATGTGKTFTMAKVIEEIGKPTLILAHNKTLAAQLCNEIRSFF
PNNAVEYFISYYDYYQPEAYIPVTDTFIEKTASINEEIDMLRHSATRSLFERQDVIVVASISCIYGLGIPAEYLKASIPL
KVGAEVDQRQLLRNLAAVQYTRNDVDLGRGRFRVKGDVLEIGPAYEDRIIRVEFFGDEIDAIRYVDPVTGETLQSLEGLN
VYPARHFVTPTERLEMAVEAIDAERTAQVEHLEGAGKLLEAQRLNQRTRYDLEMLREVGYCNGVENYSRHLAGRQAGEPP
ECLVDYFPKDWLLIVDESHVTVPQIRGMYNGDQARKKVLIDHGFRLPSAADNRPLKSEEFWQKVGQCVFVSATPGLWEIE
LSENRVVEQVIRPTGVVDPEIFVRPTTGQVDDLLAEIQERVGRQERTLITTLTKRMAEDLTEYFEERGVRVRYLHSEINA
IQRIEILRDLREGVFDVLIGVNLLREGLDLPEVSLVAILDADKEGFLRAERSLIQTIGRAARHVRGQAILYADNLTDSME
KAISETERRRKIQLAYNKKHGITPQPVKKGSDNAILAFLEVSRRLNTQELEDAYEQADDLPLESVPELITQLEAQMKEAA
KNLEFEEAAKYRDRIKNLRSKLLGQQSP
>Mature_667_residues
TAFDLHAPFVPKGDQPQAIEKLTTHLQSGHSRQTLLGATGTGKTFTMAKVIEEIGKPTLILAHNKTLAAQLCNEIRSFFP
NNAVEYFISYYDYYQPEAYIPVTDTFIEKTASINEEIDMLRHSATRSLFERQDVIVVASISCIYGLGIPAEYLKASIPLK
VGAEVDQRQLLRNLAAVQYTRNDVDLGRGRFRVKGDVLEIGPAYEDRIIRVEFFGDEIDAIRYVDPVTGETLQSLEGLNV
YPARHFVTPTERLEMAVEAIDAERTAQVEHLEGAGKLLEAQRLNQRTRYDLEMLREVGYCNGVENYSRHLAGRQAGEPPE
CLVDYFPKDWLLIVDESHVTVPQIRGMYNGDQARKKVLIDHGFRLPSAADNRPLKSEEFWQKVGQCVFVSATPGLWEIEL
SENRVVEQVIRPTGVVDPEIFVRPTTGQVDDLLAEIQERVGRQERTLITTLTKRMAEDLTEYFEERGVRVRYLHSEINAI
QRIEILRDLREGVFDVLIGVNLLREGLDLPEVSLVAILDADKEGFLRAERSLIQTIGRAARHVRGQAILYADNLTDSMEK
AISETERRRKIQLAYNKKHGITPQPVKKGSDNAILAFLEVSRRLNTQELEDAYEQADDLPLESVPELITQLEAQMKEAAK
NLEFEEAAKYRDRIKNLRSKLLGQQSP

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain

Homologues:

Organism=Escherichia coli, GI1786996, Length=666, Percent_Identity=57.2072072072072, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=228, Percent_Identity=23.6842105263158, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UVRB_ACAM1 (B0CE09)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519802.1
- ProteinModelPortal:   B0CE09
- GeneID:   5684320
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5529
- HOGENOM:   HBG703949
- OMA:   AAQLCNE
- ProtClustDB:   PRK05298
- BioCyc:   AMAR329726:AM1_5529-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00204
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00631

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR; SSF46600 UvrB_C

EC number: NA

Molecular weight: Translated: 75747; Mature: 75616

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFDLHAPFVPKGDQPQAIEKLTTHLQSGHSRQTLLGATGTGKTFTMAKVIEEIGKPTL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEE
ILAHNKTLAAQLCNEIRSFFPNNAVEYFISYYDYYQPEAYIPVTDTFIEKTASINEEIDM
EEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHCCHHHHHH
LRHSATRSLFERQDVIVVASISCIYGLGIPAEYLKASIPLKVGAEVDQRQLLRNLAAVQY
HHHHHHHHHHHHCCEEEEEEHHHHHHCCCCHHHHHCCCCEEECCCCHHHHHHHHHHHHHH
TRNDVDLGRGRFRVKGDVLEIGPAYEDRIIRVEFFGDEIDAIRYVDPVTGETLQSLEGLN
CCCCCCCCCCEEEEECCEEECCCCCCCCEEEEEEECCCCCHHHCCCCCCHHHHHHHCCCC
VYPARHFVTPTERLEMAVEAIDAERTAQVEHLEGAGKLLEAQRLNQRTRYDLEMLREVGY
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
CNGVENYSRHLAGRQAGEPPECLVDYFPKDWLLIVDESHVTVPQIRGMYNGDQARKKVLI
CCCHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHH
DHGFRLPSAADNRPLKSEEFWQKVGQCVFVSATPGLWEIELSENRVVEQVIRPTGVVDPE
HCCCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCEEEEEECCHHHHHHHHCCCCCCCCC
IFVRPTTGQVDDLLAEIQERVGRQERTLITTLTKRMAEDLTEYFEERGVRVRYLHSEINA
EEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHH
IQRIEILRDLREGVFDVLIGVNLLREGLDLPEVSLVAILDADKEGFLRAERSLIQTIGRA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH
ARHVRGQAILYADNLTDSMEKAISETERRRKIQLAYNKKHGITPQPVKKGSDNAILAFLE
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCEEHHHH
VSRRLNTQELEDAYEQADDLPLESVPELITQLEAQMKEAAKNLEFEEAAKYRDRIKNLRS
HHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KLLGQQSP
HHCCCCCC
>Mature Secondary Structure 
TAFDLHAPFVPKGDQPQAIEKLTTHLQSGHSRQTLLGATGTGKTFTMAKVIEEIGKPTL
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEE
ILAHNKTLAAQLCNEIRSFFPNNAVEYFISYYDYYQPEAYIPVTDTFIEKTASINEEIDM
EEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHCCHHHHHH
LRHSATRSLFERQDVIVVASISCIYGLGIPAEYLKASIPLKVGAEVDQRQLLRNLAAVQY
HHHHHHHHHHHHCCEEEEEEHHHHHHCCCCHHHHHCCCCEEECCCCHHHHHHHHHHHHHH
TRNDVDLGRGRFRVKGDVLEIGPAYEDRIIRVEFFGDEIDAIRYVDPVTGETLQSLEGLN
CCCCCCCCCCEEEEECCEEECCCCCCCCEEEEEEECCCCCHHHCCCCCCHHHHHHHCCCC
VYPARHFVTPTERLEMAVEAIDAERTAQVEHLEGAGKLLEAQRLNQRTRYDLEMLREVGY
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
CNGVENYSRHLAGRQAGEPPECLVDYFPKDWLLIVDESHVTVPQIRGMYNGDQARKKVLI
CCCHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHH
DHGFRLPSAADNRPLKSEEFWQKVGQCVFVSATPGLWEIELSENRVVEQVIRPTGVVDPE
HCCCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCEEEEEECCHHHHHHHHCCCCCCCCC
IFVRPTTGQVDDLLAEIQERVGRQERTLITTLTKRMAEDLTEYFEERGVRVRYLHSEINA
EEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHH
IQRIEILRDLREGVFDVLIGVNLLREGLDLPEVSLVAILDADKEGFLRAERSLIQTIGRA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH
ARHVRGQAILYADNLTDSMEKAISETERRRKIQLAYNKKHGITPQPVKKGSDNAILAFLE
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCEEHHHH
VSRRLNTQELEDAYEQADDLPLESVPELITQLEAQMKEAAKNLEFEEAAKYRDRIKNLRS
HHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KLLGQQSP
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA