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The map label for this gene is ndhM [H]

Identifier: 158338476

GI number: 158338476

Start: 5437296

End: 5438885

Strand: Reverse

Name: ndhM [H]

Synonym: AM1_5378

Alternate gene names: 158338476

Gene position: 5438885-5437296 (Counterclockwise)

Preceding gene: 158338477

Following gene: 158338475

Centisome position: 83.63

GC content: 47.61

Gene sequence:

>1590_bases
ATGCTCAGTGTCCTCGTATGGTTCCCCCTATTTGGAGTTCTGTTATTAGCACTTTTGCCCAAAAGCATTGATGCCAATAA
ATATGCTCGATCTGTAGCCTTAGTGGTGGCAGGTCTTGTATTTGTTTGGACAGTCTATATTGCGATTCTGTTCAATCCCA
AGCAAGTGACCATGCAATTTGGGGAATTTATTCCCTGGATTAAAGCCATTGGTCTGAACTATCACCTAGCGGTGGATGGG
TTATCTCTGCCCTTGCTTGGTCTCAATAGTCTCCTGACCTTTATCGCTATTTGGAGTACCGACCCAGAGGTCGAACGGCC
CAGATTCTATTACGCCCTGATGCTTCTCCTGAACTCCAGTGTGGCAGGAGCATTCCTAGCGCAAGATGTTCTTCTCTTTT
TCTTGTTCTATGAGTTAGAAATTATTCCTCTTTACTTCTTAATTGCCATTTGGGGGGGAGCTCGCCGATCCTACGCCGCC
ACTAAATTTCTGCTCTACACAGCCTTCTCTGGCATTGTTCTGCTTGCCACATTCCTAGGAATCGTCTGGTTGTCAGGTGC
CTCTAGCTTTGCCTATGAACCGTTAAGAGCCATTATTGTGGGGGCCTCAGATACCCCGAGTGGCTTGACCTTAAAACTTC
AGCTGATCTTGATGGTGGGGATTCTGCTGGGATTTGGCATCAAGATTCCTTTCTTCCCGTTACATACTTGGTTGCCTGAC
GCCCACGTAGAAGCTTCAACGCCTGTTTCCGTCCTTTTGGCTGGCGTGCTGCTGAAGTTAGGTACTTACGGCCTACTTCG
TTATGGTGTGGGTCTTTTGCCTGATGCCTGGGTATATTTAGGCCCCTGGTTGGCCATCTGGGCTGCGGTGAGTGCTCTTT
ATGGGACCTCTTGCGCCATCTCACAACAGGATATGAAAAAGGTGGTGGCCTATGCTTCCATTGCCCACATGGCCTTTATC
TTGCTGGCAGCAGCAGCTTCCACCGAACTGAGTATGATTGCCGCTGTCTGTCAGATGGTCAGTCACGGTTTGATTTCTGC
TTTACTGTTCTTGCTCGTTGGGGTCGTTTATAAAAAGACAGGCAGCCGAGATATGTTTTTCCTCAGGGGCTTACTCAATC
CTGAGCGAGGCTTACCCCTGATTGGTAGCATGATGATCCTAGGCGTCATGGCAAGTGCTGGAATTCCCGGCATGGTTGGT
TTTATCTCTGAATTTCTCACTTTTAGAGGAAGCTACCCCATTTTCCCTGTGCAAACGCTAGTTTGCATGATAGCGACTGG
ATTGACGGCAGTTTACTTCCTATTAATGATTAATCGGGTCTTTTTTGGCCGTTTACCAGATGAGTTAGACCGTATTCCTA
CCGTTACATGGTCTGATCGGCTTCCGGCTGCAATTTTAGTGGCACTCATCTTTCTGCTAGGGATTCAACCCAACTTTATT
GTGCGTTGGAGCGAAACTCAGATCGCTGCTTTGATTCCGTACAATCCTAGACCGCCTCAAATTATGATCACCAATATCCC
CGATCAATCGATGGCAAATGCCTCTAGCTCTATCGTGGCCTCTAGCCCACAGATAGAAATTACTGAGTAG

Upstream 100 bases:

>100_bases
GGATGATTGGTCTGCCATTGTTGGGTATTGGTCTGAACTCCTTAGTCAATAGCCCCACCATCTAATTTCTTCGCTTTAGT
TAGGAGTTTGTCTTAACCCA

Downstream 100 bases:

>100_bases
CTTTGAATTAGTTTCTGCAACTTGTTCCTCCTTATACCCCTCATCAGCTATGGTAAGCACGCCTCAAATAGAACCCTCTA
AACACCAATTGGCAGAGTTC

Product: proton-translocating NAD(P)H-quinone oxidoreductase, chain M

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 4 1; NDH-1, chain 4 1 [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MLSVLVWFPLFGVLLLALLPKSIDANKYARSVALVVAGLVFVWTVYIAILFNPKQVTMQFGEFIPWIKAIGLNYHLAVDG
LSLPLLGLNSLLTFIAIWSTDPEVERPRFYYALMLLLNSSVAGAFLAQDVLLFFLFYELEIIPLYFLIAIWGGARRSYAA
TKFLLYTAFSGIVLLATFLGIVWLSGASSFAYEPLRAIIVGASDTPSGLTLKLQLILMVGILLGFGIKIPFFPLHTWLPD
AHVEASTPVSVLLAGVLLKLGTYGLLRYGVGLLPDAWVYLGPWLAIWAAVSALYGTSCAISQQDMKKVVAYASIAHMAFI
LLAAAASTELSMIAAVCQMVSHGLISALLFLLVGVVYKKTGSRDMFFLRGLLNPERGLPLIGSMMILGVMASAGIPGMVG
FISEFLTFRGSYPIFPVQTLVCMIATGLTAVYFLLMINRVFFGRLPDELDRIPTVTWSDRLPAAILVALIFLLGIQPNFI
VRWSETQIAALIPYNPRPPQIMITNIPDQSMANASSSIVASSPQIEITE

Sequences:

>Translated_529_residues
MLSVLVWFPLFGVLLLALLPKSIDANKYARSVALVVAGLVFVWTVYIAILFNPKQVTMQFGEFIPWIKAIGLNYHLAVDG
LSLPLLGLNSLLTFIAIWSTDPEVERPRFYYALMLLLNSSVAGAFLAQDVLLFFLFYELEIIPLYFLIAIWGGARRSYAA
TKFLLYTAFSGIVLLATFLGIVWLSGASSFAYEPLRAIIVGASDTPSGLTLKLQLILMVGILLGFGIKIPFFPLHTWLPD
AHVEASTPVSVLLAGVLLKLGTYGLLRYGVGLLPDAWVYLGPWLAIWAAVSALYGTSCAISQQDMKKVVAYASIAHMAFI
LLAAAASTELSMIAAVCQMVSHGLISALLFLLVGVVYKKTGSRDMFFLRGLLNPERGLPLIGSMMILGVMASAGIPGMVG
FISEFLTFRGSYPIFPVQTLVCMIATGLTAVYFLLMINRVFFGRLPDELDRIPTVTWSDRLPAAILVALIFLLGIQPNFI
VRWSETQIAALIPYNPRPPQIMITNIPDQSMANASSSIVASSPQIEITE
>Mature_529_residues
MLSVLVWFPLFGVLLLALLPKSIDANKYARSVALVVAGLVFVWTVYIAILFNPKQVTMQFGEFIPWIKAIGLNYHLAVDG
LSLPLLGLNSLLTFIAIWSTDPEVERPRFYYALMLLLNSSVAGAFLAQDVLLFFLFYELEIIPLYFLIAIWGGARRSYAA
TKFLLYTAFSGIVLLATFLGIVWLSGASSFAYEPLRAIIVGASDTPSGLTLKLQLILMVGILLGFGIKIPFFPLHTWLPD
AHVEASTPVSVLLAGVLLKLGTYGLLRYGVGLLPDAWVYLGPWLAIWAAVSALYGTSCAISQQDMKKVVAYASIAHMAFI
LLAAAASTELSMIAAVCQMVSHGLISALLFLLVGVVYKKTGSRDMFFLRGLLNPERGLPLIGSMMILGVMASAGIPGMVG
FISEFLTFRGSYPIFPVQTLVCMIATGLTAVYFLLMINRVFFGRLPDELDRIPTVTWSDRLPAAILVALIFLLGIQPNFI
VRWSETQIAALIPYNPRPPQIMITNIPDQSMANASSSIVASSPQIEITE

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=391, Percent_Identity=29.156010230179, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI251831117, Length=373, Percent_Identity=24.1286863270777, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1788613, Length=400, Percent_Identity=38.5, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1788827, Length=302, Percent_Identity=27.8145695364238, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788831, Length=447, Percent_Identity=27.2930648769575, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788829, Length=391, Percent_Identity=27.3657289002558, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788614, Length=493, Percent_Identity=26.7748478701826, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI145693160, Length=351, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI2367154, Length=199, Percent_Identity=27.1356783919598, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 57778; Mature: 57778

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVLVWFPLFGVLLLALLPKSIDANKYARSVALVVAGLVFVWTVYIAILFNPKQVTMQF
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GEFIPWIKAIGLNYHLAVDGLSLPLLGLNSLLTFIAIWSTDPEVERPRFYYALMLLLNSS
HHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
VAGAFLAQDVLLFFLFYELEIIPLYFLIAIWGGARRSYAATKFLLYTAFSGIVLLATFLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IVWLSGASSFAYEPLRAIIVGASDTPSGLTLKLQLILMVGILLGFGIKIPFFPLHTWLPD
HHHHCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHCCCC
AHVEASTPVSVLLAGVLLKLGTYGLLRYGVGLLPDAWVYLGPWLAIWAAVSALYGTSCAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
SQQDMKKVVAYASIAHMAFILLAAAASTELSMIAAVCQMVSHGLISALLFLLVGVVYKKT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSRDMFFLRGLLNPERGLPLIGSMMILGVMASAGIPGMVGFISEFLTFRGSYPIFPVQTL
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHH
VCMIATGLTAVYFLLMINRVFFGRLPDELDRIPTVTWSDRLPAAILVALIFLLGIQPNFI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
VRWSETQIAALIPYNPRPPQIMITNIPDQSMANASSSIVASSPQIEITE
EEECCCCEEEEECCCCCCCEEEEECCCCHHHHCCCCCCEECCCCEEECC
>Mature Secondary Structure
MLSVLVWFPLFGVLLLALLPKSIDANKYARSVALVVAGLVFVWTVYIAILFNPKQVTMQF
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GEFIPWIKAIGLNYHLAVDGLSLPLLGLNSLLTFIAIWSTDPEVERPRFYYALMLLLNSS
HHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
VAGAFLAQDVLLFFLFYELEIIPLYFLIAIWGGARRSYAATKFLLYTAFSGIVLLATFLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IVWLSGASSFAYEPLRAIIVGASDTPSGLTLKLQLILMVGILLGFGIKIPFFPLHTWLPD
HHHHCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHCCCC
AHVEASTPVSVLLAGVLLKLGTYGLLRYGVGLLPDAWVYLGPWLAIWAAVSALYGTSCAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
SQQDMKKVVAYASIAHMAFILLAAAASTELSMIAAVCQMVSHGLISALLFLLVGVVYKKT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSRDMFFLRGLLNPERGLPLIGSMMILGVMASAGIPGMVGFISEFLTFRGSYPIFPVQTL
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHH
VCMIATGLTAVYFLLMINRVFFGRLPDELDRIPTVTWSDRLPAAILVALIFLLGIQPNFI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
VRWSETQIAALIPYNPRPPQIMITNIPDQSMANASSSIVASSPQIEITE
EEECCCCEEEEECCCCCCCEEEEECCCCHHHHCCCCCCEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]