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The map label for this gene is ndhF [H]

Identifier: 158338477

GI number: 158338477

Start: 5438934

End: 5440835

Strand: Reverse

Name: ndhF [H]

Synonym: AM1_5379

Alternate gene names: 158338477

Gene position: 5440835-5438934 (Counterclockwise)

Preceding gene: 158338486

Following gene: 158338476

Centisome position: 83.66

GC content: 48.84

Gene sequence:

>1902_bases
GTGCTGTCGCAATTTCTTCTTGAACATATCTGGTGGGTTCCCTTTTTTGGATTACTAGGAGCTCTTTTCTCGCTCCCCTG
GTCCTTGGGATTAATCCGGCGAAGTGGGCCCCGCCCAGTCGCTTATTTCAACATTTTGATGACGTTGTTAAGTTGCTTGC
ATGGCACGATTGTTTTCAAGCAAGTTCTAGGCCAACCCCCTCAACAACTGGTCTATGAATGGCTGCAAGTTGCGGACTTA
AATTTAACCTTTGCGATTGAAATTTCCCCTGTCAGTATTGGTGCCCTAGAACTGGTCACTTTTCTGAGCTTACTCACCCA
AGTCTTTGCGATTGGCTATATGGACAAGGACTGGTCTGTCGCTCGTTTTTTTGGATTGATGGGCTTTTTCGAAGGAGCCC
TTGCAGGTATCGCCATTAGCGACTCGCTGATTCTTAGCTATGCTTTGTTGGAAATGTTGACGTTTTCTAGCTACCTGTTG
GTGGGCTTTTGGTATGCTCAGCCGCTGGTGGTCACGGCAGCTCGGGATGCTTTTCTGACCAAGCGAGTCGGTGACATTAT
CCTCTTAATGGGGTTAGTTGCTTTGACCAGCTATGGTGCTGGACTAACCTTTTCAGACCTGAGGGCCTGGAGTGAAGCCA
CTGCTTTACCTCCCCTAACGGATGCTTTGCTGGGATTGGCCTTAATTGCAGGCCCTACAGGGAATTGTGCTCAATTCCCT
TTAAACCTCTGGCTGGATGAAGCCATGGAAGGGCCGAATCCTGCAGGGGTCATGCGGAACACTGCAGTTGTCTCCATCGG
CGCTTACTTTCTGATTAAGCTGCAGCCTATTTTTAGCCTGTCTTTGATTGCTTCCGATACCTTACTGATTATTGGTGGCG
TCACAGCGGTTGGCGCTTCCTTGATATCCATCGCTCAGGTGGATATCAAGCGAGCATTGTCCCATTCCACCAGTGCCTAT
TTGGGTTTGGTATTTATTGCCGTGGGCTTGGGGCAAGTAAACATTGCTCTATCATTGCTGCTCACCCATGCGATTGCAAA
AGCGCTTCTCTTCTTCAGCATCAGTTCCGTCATTTTGGGCACGAATAGTCAGGACATGACGGAAATGGGAGGGCTGTGGT
CGCGACTGCCTGCTACCACTACTGCCTTTGTCGTGGGCGCTGCCAGTTTAGTGGTGATTCTGCCGATGGGCTTTTTCCGA
ATGATGCAGCAATGGCTGACGGGCGGGTGGAATATGCCCGTTTGGCTGTTTGTTCTGTTGGTCTTTGTGAATACCACCAG
CGCCATCAGTCTCACTCGGATTTTTCGGTTAATCTTTCTGGGGCAACGCCAACCTAAGACTCGACGGGTACCTGAAGTGC
CCTGGCTGATGGCACTCCCGATGGTGACTATGACTGTGGCTGTGTTGGCTTTCCCACTGATGCCATTGAAATGGTCCCTG
TGGCTCCATCCTCTTCCATCTGCTGTAGTCTTTAGTGAATATTCCATCCCTTACGGCCTCCCCATTATGGTGGCATCAGG
TGTGCTCGGTGTTGTGATAGGCTCTGTGTTTGAACTGAGACGTTCTCTCTCTCGACCGACCACGTTTTATCTACGCTTTT
TTCAGGATCTGTTGGCCTACGACTTTTACATCGAGCGGGTGTATGAATTCACTGTAGTTGCCATTGTTCGCCTTTCCTCT
GAATTCACAACTTATCTAGATAAATATGTTGTTGATGGAATCGTTAATTTCGTAGGTAAAGCTACCTTGTTGAGTGGACA
AGGTCTCAAATACGGGGTCTCTGGCCAATCTCAATCCTATTTGCTCACGATCATGATCGGCATCAGTGCCATTTTATGGC
TACTGCTCAGGGATGATTGGTCTGCCATTGTTGGGTATTGGTCTGAACTCCTTAGTCAATAG

Upstream 100 bases:

>100_bases
TTATCTGAAATTTCTGACTGTATTGAGCAGATAAAGATTTACAGTTTTAAGTTAGTTGTCAGTCTATCAACAACCATTGC
AAACCCTAATTGACTAAGTC

Downstream 100 bases:

>100_bases
CCCCACCATCTAATTTCTTCGCTTTAGTTAGGAGTTTGTCTTAACCCAATGCTCAGTGTCCTCGTATGGTTCCCCCTATT
TGGAGTTCTGTTATTAGCAC

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MLSQFLLEHIWWVPFFGLLGALFSLPWSLGLIRRSGPRPVAYFNILMTLLSCLHGTIVFKQVLGQPPQQLVYEWLQVADL
NLTFAIEISPVSIGALELVTFLSLLTQVFAIGYMDKDWSVARFFGLMGFFEGALAGIAISDSLILSYALLEMLTFSSYLL
VGFWYAQPLVVTAARDAFLTKRVGDIILLMGLVALTSYGAGLTFSDLRAWSEATALPPLTDALLGLALIAGPTGNCAQFP
LNLWLDEAMEGPNPAGVMRNTAVVSIGAYFLIKLQPIFSLSLIASDTLLIIGGVTAVGASLISIAQVDIKRALSHSTSAY
LGLVFIAVGLGQVNIALSLLLTHAIAKALLFFSISSVILGTNSQDMTEMGGLWSRLPATTTAFVVGAASLVVILPMGFFR
MMQQWLTGGWNMPVWLFVLLVFVNTTSAISLTRIFRLIFLGQRQPKTRRVPEVPWLMALPMVTMTVAVLAFPLMPLKWSL
WLHPLPSAVVFSEYSIPYGLPIMVASGVLGVVIGSVFELRRSLSRPTTFYLRFFQDLLAYDFYIERVYEFTVVAIVRLSS
EFTTYLDKYVVDGIVNFVGKATLLSGQGLKYGVSGQSQSYLLTIMIGISAILWLLLRDDWSAIVGYWSELLSQ

Sequences:

>Translated_633_residues
MLSQFLLEHIWWVPFFGLLGALFSLPWSLGLIRRSGPRPVAYFNILMTLLSCLHGTIVFKQVLGQPPQQLVYEWLQVADL
NLTFAIEISPVSIGALELVTFLSLLTQVFAIGYMDKDWSVARFFGLMGFFEGALAGIAISDSLILSYALLEMLTFSSYLL
VGFWYAQPLVVTAARDAFLTKRVGDIILLMGLVALTSYGAGLTFSDLRAWSEATALPPLTDALLGLALIAGPTGNCAQFP
LNLWLDEAMEGPNPAGVMRNTAVVSIGAYFLIKLQPIFSLSLIASDTLLIIGGVTAVGASLISIAQVDIKRALSHSTSAY
LGLVFIAVGLGQVNIALSLLLTHAIAKALLFFSISSVILGTNSQDMTEMGGLWSRLPATTTAFVVGAASLVVILPMGFFR
MMQQWLTGGWNMPVWLFVLLVFVNTTSAISLTRIFRLIFLGQRQPKTRRVPEVPWLMALPMVTMTVAVLAFPLMPLKWSL
WLHPLPSAVVFSEYSIPYGLPIMVASGVLGVVIGSVFELRRSLSRPTTFYLRFFQDLLAYDFYIERVYEFTVVAIVRLSS
EFTTYLDKYVVDGIVNFVGKATLLSGQGLKYGVSGQSQSYLLTIMIGISAILWLLLRDDWSAIVGYWSELLSQ
>Mature_633_residues
MLSQFLLEHIWWVPFFGLLGALFSLPWSLGLIRRSGPRPVAYFNILMTLLSCLHGTIVFKQVLGQPPQQLVYEWLQVADL
NLTFAIEISPVSIGALELVTFLSLLTQVFAIGYMDKDWSVARFFGLMGFFEGALAGIAISDSLILSYALLEMLTFSSYLL
VGFWYAQPLVVTAARDAFLTKRVGDIILLMGLVALTSYGAGLTFSDLRAWSEATALPPLTDALLGLALIAGPTGNCAQFP
LNLWLDEAMEGPNPAGVMRNTAVVSIGAYFLIKLQPIFSLSLIASDTLLIIGGVTAVGASLISIAQVDIKRALSHSTSAY
LGLVFIAVGLGQVNIALSLLLTHAIAKALLFFSISSVILGTNSQDMTEMGGLWSRLPATTTAFVVGAASLVVILPMGFFR
MMQQWLTGGWNMPVWLFVLLVFVNTTSAISLTRIFRLIFLGQRQPKTRRVPEVPWLMALPMVTMTVAVLAFPLMPLKWSL
WLHPLPSAVVFSEYSIPYGLPIMVASGVLGVVIGSVFELRRSLSRPTTFYLRFFQDLLAYDFYIERVYEFTVVAIVRLSS
EFTTYLDKYVVDGIVNFVGKATLLSGQGLKYGVSGQSQSYLLTIMIGISAILWLLLRDDWSAIVGYWSELLSQ

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:
GO:0008137: NAD(P)H dehydrogenase, subunit NdhF3 family
GO:0016491: NAD(P)H dehydrogenase, subunit NdhF3 family
GO:0042773: NAD(P)H dehydrogenase, subunit NdhF3 family
GO:0048038: NAD(P)H dehydrogenase, subunit NdhF3 family
GO:0055114: NAD(P)H dehydrogenase, subunit NdhF3 family

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=389, Percent_Identity=28.7917737789203, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1788614, Length=460, Percent_Identity=31.0869565217391, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1788827, Length=446, Percent_Identity=24.6636771300448, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788831, Length=400, Percent_Identity=27.75, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788829, Length=431, Percent_Identity=25.9860788863109, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 69499; Mature: 69499

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00267 TACHYKININ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSQFLLEHIWWVPFFGLLGALFSLPWSLGLIRRSGPRPVAYFNILMTLLSCLHGTIVFK
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
QVLGQPPQQLVYEWLQVADLNLTFAIEISPVSIGALELVTFLSLLTQVFAIGYMDKDWSV
HHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHH
ARFFGLMGFFEGALAGIAISDSLILSYALLEMLTFSSYLLVGFWYAQPLVVTAARDAFLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
KRVGDIILLMGLVALTSYGAGLTFSDLRAWSEATALPPLTDALLGLALIAGPTGNCAQFP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHCH
LNLWLDEAMEGPNPAGVMRNTAVVSIGAYFLIKLQPIFSLSLIASDTLLIIGGVTAVGAS
HHHHHHHHCCCCCCCCHHHCHHHHHHHHHHEEEEHHHHHHHHHHCCCEEEEECHHHHHHH
LISIAQVDIKRALSHSTSAYLGLVFIAVGLGQVNIALSLLLTHAIAKALLFFSISSVILG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TNSQDMTEMGGLWSRLPATTTAFVVGAASLVVILPMGFFRMMQQWLTGGWNMPVWLFVLL
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VFVNTTSAISLTRIFRLIFLGQRQPKTRRVPEVPWLMALPMVTMTVAVLAFPLMPLKWSL
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
WLHPLPSAVVFSEYSIPYGLPIMVASGVLGVVIGSVFELRRSLSRPTTFYLRFFQDLLAY
EECCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DFYIERVYEFTVVAIVRLSSEFTTYLDKYVVDGIVNFVGKATLLSGQGLKYGVSGQSQSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCEECCCCCCCCH
LLTIMIGISAILWLLLRDDWSAIVGYWSELLSQ
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSQFLLEHIWWVPFFGLLGALFSLPWSLGLIRRSGPRPVAYFNILMTLLSCLHGTIVFK
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
QVLGQPPQQLVYEWLQVADLNLTFAIEISPVSIGALELVTFLSLLTQVFAIGYMDKDWSV
HHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHH
ARFFGLMGFFEGALAGIAISDSLILSYALLEMLTFSSYLLVGFWYAQPLVVTAARDAFLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
KRVGDIILLMGLVALTSYGAGLTFSDLRAWSEATALPPLTDALLGLALIAGPTGNCAQFP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHCH
LNLWLDEAMEGPNPAGVMRNTAVVSIGAYFLIKLQPIFSLSLIASDTLLIIGGVTAVGAS
HHHHHHHHCCCCCCCCHHHCHHHHHHHHHHEEEEHHHHHHHHHHCCCEEEEECHHHHHHH
LISIAQVDIKRALSHSTSAYLGLVFIAVGLGQVNIALSLLLTHAIAKALLFFSISSVILG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TNSQDMTEMGGLWSRLPATTTAFVVGAASLVVILPMGFFRMMQQWLTGGWNMPVWLFVLL
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VFVNTTSAISLTRIFRLIFLGQRQPKTRRVPEVPWLMALPMVTMTVAVLAFPLMPLKWSL
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
WLHPLPSAVVFSEYSIPYGLPIMVASGVLGVVIGSVFELRRSLSRPTTFYLRFFQDLLAY
EECCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DFYIERVYEFTVVAIVRLSSEFTTYLDKYVVDGIVNFVGKATLLSGQGLKYGVSGQSQSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCEECCCCCCCCH
LLTIMIGISAILWLLLRDDWSAIVGYWSELLSQ
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]