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The map label for this gene is ymfC [C]

Identifier: 158338459

GI number: 158338459

Start: 5419865

End: 5420461

Strand: Reverse

Name: ymfC [C]

Synonym: AM1_5361

Alternate gene names: 158338459

Gene position: 5420461-5419865 (Counterclockwise)

Preceding gene: 158338466

Following gene: 158338458

Centisome position: 83.34

GC content: 50.92

Gene sequence:

>597_bases
ATGGCTTGGCAACATATCTTGTTCTATAAGCCCTACGGGGTCCTTAGCCAGTTTACGGATCGACAAGAAACGTCGCATCC
AACGTTAAAAGAGTTTATTCCGATCCCAGATATTTATCCGGTTGGGCGATTGGATAGGGATAGTGAAGGGTTACTCTTGC
TGTCCAATCATGGTGGTCTTCAGCATCAGTTATGTAATCCTAAATTTGGACATCCCCGGACTTATTGGGTTCAGGTGGAG
AACATTCCCGATCAGATTGCGTTACAAAAGCTTAAGCAGGGGGTCACGATCCGAAACTATCGAACGCGGCCGACTCAAGC
CTCACTGTTATCTGAGCCACCCCTATTACCCCCTCGGACTCCACCAGTCCGTTTTCGCAAGCATATCCCCACGGCATGGA
TGACAATGACGCTCTATGAGGGCCGAAACCGACAGGTTCGTCGGATGACGGCTGCTGTGGGGTTCCCGACCTTACGTTTG
GTACGGGTTGCGATCGCACATTTGCAACTCCAGGATCTTCAGCCAGGCCAATGGCGCCACCTATCCCCCGCAGAAATCGA
ACAACTCCAATTCCTGACCCATGTATCTCGTCTCTAG

Upstream 100 bases:

>100_bases
CTCATGCCATAACTTGAGGTGTTTGGAAACGAGCGCTGATGTTCTATACCTATAGGGAATGTCAGATGTATTCAGTGAAA
TTGAAATCTAACGGACAGCA

Downstream 100 bases:

>100_bases
CCCAAGAGACCAAACCCGATTCGTCGGCACACTCAAAAACATTTCAAATTTCAATCTACAGTTGAAGATAATTGTGAGTT
GTATAGTGGGATCACTCAAT

Product: pseudouridine synthase family 1 protein

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MAWQHILFYKPYGVLSQFTDRQETSHPTLKEFIPIPDIYPVGRLDRDSEGLLLLSNHGGLQHQLCNPKFGHPRTYWVQVE
NIPDQIALQKLKQGVTIRNYRTRPTQASLLSEPPLLPPRTPPVRFRKHIPTAWMTMTLYEGRNRQVRRMTAAVGFPTLRL
VRVAIAHLQLQDLQPGQWRHLSPAEIEQLQFLTHVSRL

Sequences:

>Translated_198_residues
MAWQHILFYKPYGVLSQFTDRQETSHPTLKEFIPIPDIYPVGRLDRDSEGLLLLSNHGGLQHQLCNPKFGHPRTYWVQVE
NIPDQIALQKLKQGVTIRNYRTRPTQASLLSEPPLLPPRTPPVRFRKHIPTAWMTMTLYEGRNRQVRRMTAAVGFPTLRL
VRVAIAHLQLQDLQPGQWRHLSPAEIEQLQFLTHVSRL
>Mature_197_residues
AWQHILFYKPYGVLSQFTDRQETSHPTLKEFIPIPDIYPVGRLDRDSEGLLLLSNHGGLQHQLCNPKFGHPRTYWVQVEN
IPDQIALQKLKQGVTIRNYRTRPTQASLLSEPPLLPPRTPPVRFRKHIPTAWMTMTLYEGRNRQVRRMTAAVGFPTLRLV
RVAIAHLQLQDLQPGQWRHLSPAEIEQLQFLTHVSRL

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=57.0621468926554, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI1788510, Length=198, Percent_Identity=30.8080808080808, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790453, Length=185, Percent_Identity=28.1081081081081, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 23019; Mature: 22888

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWQHILFYKPYGVLSQFTDRQETSHPTLKEFIPIPDIYPVGRLDRDSEGLLLLSNHGGL
CCCCEEEEECCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCC
QHQLCNPKFGHPRTYWVQVENIPDQIALQKLKQGVTIRNYRTRPTQASLLSEPPLLPPRT
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCHHHHHCCCCCCCCCC
PPVRFRKHIPTAWMTMTLYEGRNRQVRRMTAAVGFPTLRLVRVAIAHLQLQDLQPGQWRH
CCHHHHHHCCHHHEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
LSPAEIEQLQFLTHVSRL
CCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AWQHILFYKPYGVLSQFTDRQETSHPTLKEFIPIPDIYPVGRLDRDSEGLLLLSNHGGL
CCCEEEEECCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCC
QHQLCNPKFGHPRTYWVQVENIPDQIALQKLKQGVTIRNYRTRPTQASLLSEPPLLPPRT
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCHHHHHCCCCCCCCCC
PPVRFRKHIPTAWMTMTLYEGRNRQVRRMTAAVGFPTLRLVRVAIAHLQLQDLQPGQWRH
CCHHHHHHCCHHHEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
LSPAEIEQLQFLTHVSRL
CCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]