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The map label for this gene is spkB [H]

Identifier: 158338403

GI number: 158338403

Start: 5365377

End: 5366513

Strand: Direct

Name: spkB [H]

Synonym: AM1_5304

Alternate gene names: 158338403

Gene position: 5365377-5366513 (Clockwise)

Preceding gene: 158338402

Following gene: 158338405

Centisome position: 82.5

GC content: 48.11

Gene sequence:

>1137_bases
ATGGTGTATTGTCCCAATCCTAAGTGTAAACAGCGGCAAAATCCTGCAACGGCGGAGATCTGTCAAAGTTGCGAAACACC
ATTAATTCTTAACCAGAACTATCGTCTGATTCGGCCCTTAGTGAACCTTGAGCGATCGGGCAACACTGAAGTGTTTGAAA
TTGAGGATCTGCGCTCATCGGATTCAGCGCCGAAAGTGCTGAAGCTTCTCAAGTACAATGGCGGCGATCTAGAGCGGTTG
TTTAAACAAGAGGCAATCTATTTACAGAGCCTGGACCATCCTGCAATTCCCAAAATTGATCCTCAAGACCGTGCAGGCGA
TGGCTATTTTTCAGTTGTGGAGCCAGCCACTCGTCGAGAAATCCATTATTTCGTGATGGAAAAGGTCGAAGGGAAGAATC
TACGAGACTGGCTACAGGAGCAAGCGCCGATTGATAGTGCCACTCTTCTGAAGTGGCTTCGGGAATTACTGACTATTTTG
GACTATCTGCATGCCCAAAATATTTGGCATCGAGATATTAAACCTTCCAATATCATGTTGCGACCGGACGGACAGTTGGT
TCTGATTGACTTTGGCGCAGTCAAACAAGTTCGGCCTCACCCAGAAGAGGCTGGAGATATTCCCCCTCGACCGGATATAA
CGGTTACTGATACCTGTGTGTTTGCGGCGGGATATACCCCCCCAGAACAAATGGATGGGAGAACGGTTCAGCAGTCTGAT
TTTTTCGCTTTGGGGCGTAGCTGTGTGCATTTACTCACCCAAATTCCGCCCTACAATCTGCGGGTCGATGATGGTGGTCA
ATTAGTCTGGCGAGAGAAAGTCCCTGATGTTTCGGTGGTGCTTGCTGATTGGATTGATCAACTGATGTCTCCGTTGGTTC
GAGACCGTCCTCAAACTGCAGCTGAGGTCTTGAAACACATTCAACCAGACAACCTTGTTTCCCCTCCCCAGGCCCAAAAT
TTATCTGAAGGAGAGATTTCACAAAGTTCTTTCCCTTGGGGCTCCTTGCTGAACCTTATTCTGTTCTCCATCCTGCTCAT
TAGTGGCCTGTTGTGGTGGCAAGCCCATGAGAATAGTCAACCAGCTGAGGAGACAAATCAACAGGTGCAGCATCATCTTC
CATCCCTGAGACTATGA

Upstream 100 bases:

>100_bases
CAGCGGTCTCTGATTGAACATGTGGATCAGGGGTTTATCCTGCCGCCAGTGATCAAAGAGGTCACCCAACAAATTATGGT
GTCTGAAGCCTAATCGATAG

Downstream 100 bases:

>100_bases
AGGGGGATTCAGTAGCTGGTCGGTTTCTGATGTTGCTTTTTCCATGATTTCTTCGACAGCCTGATCAGAGGTGTCAGGAT
TAAGAGCTTGACTAAGAGCC

Product: serine/threonine protein kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MVYCPNPKCKQRQNPATAEICQSCETPLILNQNYRLIRPLVNLERSGNTEVFEIEDLRSSDSAPKVLKLLKYNGGDLERL
FKQEAIYLQSLDHPAIPKIDPQDRAGDGYFSVVEPATRREIHYFVMEKVEGKNLRDWLQEQAPIDSATLLKWLRELLTIL
DYLHAQNIWHRDIKPSNIMLRPDGQLVLIDFGAVKQVRPHPEEAGDIPPRPDITVTDTCVFAAGYTPPEQMDGRTVQQSD
FFALGRSCVHLLTQIPPYNLRVDDGGQLVWREKVPDVSVVLADWIDQLMSPLVRDRPQTAAEVLKHIQPDNLVSPPQAQN
LSEGEISQSSFPWGSLLNLILFSILLISGLLWWQAHENSQPAEETNQQVQHHLPSLRL

Sequences:

>Translated_378_residues
MVYCPNPKCKQRQNPATAEICQSCETPLILNQNYRLIRPLVNLERSGNTEVFEIEDLRSSDSAPKVLKLLKYNGGDLERL
FKQEAIYLQSLDHPAIPKIDPQDRAGDGYFSVVEPATRREIHYFVMEKVEGKNLRDWLQEQAPIDSATLLKWLRELLTIL
DYLHAQNIWHRDIKPSNIMLRPDGQLVLIDFGAVKQVRPHPEEAGDIPPRPDITVTDTCVFAAGYTPPEQMDGRTVQQSD
FFALGRSCVHLLTQIPPYNLRVDDGGQLVWREKVPDVSVVLADWIDQLMSPLVRDRPQTAAEVLKHIQPDNLVSPPQAQN
LSEGEISQSSFPWGSLLNLILFSILLISGLLWWQAHENSQPAEETNQQVQHHLPSLRL
>Mature_378_residues
MVYCPNPKCKQRQNPATAEICQSCETPLILNQNYRLIRPLVNLERSGNTEVFEIEDLRSSDSAPKVLKLLKYNGGDLERL
FKQEAIYLQSLDHPAIPKIDPQDRAGDGYFSVVEPATRREIHYFVMEKVEGKNLRDWLQEQAPIDSATLLKWLRELLTIL
DYLHAQNIWHRDIKPSNIMLRPDGQLVLIDFGAVKQVRPHPEEAGDIPPRPDITVTDTCVFAAGYTPPEQMDGRTVQQSD
FFALGRSCVHLLTQIPPYNLRVDDGGQLVWREKVPDVSVVLADWIDQLMSPLVRDRPQTAAEVLKHIQPDNLVSPPQAQN
LSEGEISQSSFPWGSLLNLILFSILLISGLLWWQAHENSQPAEETNQQVQHHLPSLRL

Specific function: Protein kinase required for cell motility, but not for phototaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI310112945, Length=217, Percent_Identity=26.7281105990783, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI4505695, Length=172, Percent_Identity=26.7441860465116, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17566666, Length=158, Percent_Identity=31.6455696202532, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133900709, Length=201, Percent_Identity=26.3681592039801, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133900707, Length=201, Percent_Identity=26.3681592039801, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319502, Length=183, Percent_Identity=27.3224043715847, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325047, Length=198, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324472, Length=269, Percent_Identity=23.7918215613383, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17864254, Length=191, Percent_Identity=29.3193717277487, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644584, Length=191, Percent_Identity=29.3193717277487, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644586, Length=191, Percent_Identity=29.3193717277487, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221378052, Length=191, Percent_Identity=29.3193717277487, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001646
- InterPro:   IPR020636
- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR017441
- InterPro:   IPR017442
- InterPro:   IPR016252 [H]

Pfam domain/function: PF00805 Pentapeptide; PF00069 Pkinase [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 43006; Mature: 43006

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00108 PROTEIN_KINASE_ST ; PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYCPNPKCKQRQNPATAEICQSCETPLILNQNYRLIRPLVNLERSGNTEVFEIEDLRSS
CCCCCCCCHHCCCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHCCCCCCCEEEECCCCCC
DSAPKVLKLLKYNGGDLERLFKQEAIYLQSLDHPAIPKIDPQDRAGDGYFSVVEPATRRE
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCHHHHH
IHYFVMEKVEGKNLRDWLQEQAPIDSATLLKWLRELLTILDYLHAQNIWHRDIKPSNIML
HHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEE
RPDGQLVLIDFGAVKQVRPHPEEAGDIPPRPDITVTDTCVFAAGYTPPEQMDGRTVQQSD
CCCCCEEEEECCCCCCCCCCCHHCCCCCCCCCCEEECCEEEECCCCCHHHHCCCCCCHHH
FFALGRSCVHLLTQIPPYNLRVDDGGQLVWREKVPDVSVVLADWIDQLMSPLVRDRPQTA
HHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
AEVLKHIQPDNLVSPPQAQNLSEGEISQSSFPWGSLLNLILFSILLISGLLWWQAHENSQ
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PAEETNQQVQHHLPSLRL
CHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVYCPNPKCKQRQNPATAEICQSCETPLILNQNYRLIRPLVNLERSGNTEVFEIEDLRSS
CCCCCCCCHHCCCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHCCCCCCCEEEECCCCCC
DSAPKVLKLLKYNGGDLERLFKQEAIYLQSLDHPAIPKIDPQDRAGDGYFSVVEPATRRE
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCHHHHH
IHYFVMEKVEGKNLRDWLQEQAPIDSATLLKWLRELLTILDYLHAQNIWHRDIKPSNIML
HHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEE
RPDGQLVLIDFGAVKQVRPHPEEAGDIPPRPDITVTDTCVFAAGYTPPEQMDGRTVQQSD
CCCCCEEEEECCCCCCCCCCCHHCCCCCCCCCCEEECCEEEECCCCCHHHHCCCCCCHHH
FFALGRSCVHLLTQIPPYNLRVDDGGQLVWREKVPDVSVVLADWIDQLMSPLVRDRPQTA
HHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
AEVLKHIQPDNLVSPPQAQNLSEGEISQSSFPWGSLLNLILFSILLISGLLWWQAHENSQ
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PAEETNQQVQHHLPSLRL
CHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12732981; 8905231 [H]