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The map label for this gene is gltS [H]

Identifier: 158338368

GI number: 158338368

Start: 5334060

End: 5335250

Strand: Reverse

Name: gltS [H]

Synonym: AM1_5264

Alternate gene names: 158338368

Gene position: 5335250-5334060 (Counterclockwise)

Preceding gene: 158338369

Following gene: 158338367

Centisome position: 82.03

GC content: 49.2

Gene sequence:

>1191_bases
ATGATTGAGACCGTTGTTGACATTGACTGTTTAACCTTTCTACTAATCAGCATTTTTATTCTTGGGATTGGCATCCTTCT
GACCCGCACCCTCCCTCTCCTCAGAACCCTGAATATTCCTGCTGCCGTTTCGGGAGGACTTATTGGCTGTGGCCTGGTGG
CCTATGTCAACCTATGCACACCTTGGCATTTACGGTTTGATCTGAGCTTGCGCGACAACCTGCTGTTGATCTTCTTTAGC
ACGATTGGCCTAGCTGCTCGCTTTAAGCAGCTGAAAGAAGGGGGAAAAACCCTTGCTGCTGTGATTGGGGTGATGATTGC
CTTTTTATTCCTGCAAAATGCAGTCGGGATCGGGGTGGCCTGCTTAGTCGGTTGCCCTCCTATTTTTGGACTGCTCGGAG
GGACGATTGCCTTTGTAGGGGGTCATGGTACAGCGATCACCTGGGGAACATTGCTTGAGGCCCAAGGGTGGACTGGTGCA
TTGGAATTTAGCTTAGCAGCTGCCACCTTAGGGCTGATTGTGGGAGGGGTTTTCGCGGGACCCATTAGTCAGCATTTGAT
TCGCCGGTTTGAGCTGGAGTCCCTATCCCCTCCCCCTGATGTTGTGGCGGAAAGCCCAGTAACGATGCCAACGAGTTTGG
AAGGCATGTTTGGGATTGTAATGTTAATTGCGACATCCCTAAGTGTGGGACTGGGAATACATCTGTTCACGCTGGATTGG
GGCATTGTTTTGCCCCGATTTTTGCCTATTATGCTGGTGGCTATTCTCCTGATCAATCTCCTAGATTGGGGAAATATTTC
GATCAACGATACAGCCCTGTCCTTTTGGAATGACGTTAGCCTGCAGCTTTTCCTGGTCATGAGTTTGATGAACTTGCAAA
TCCTGTCATGGATGCAAACGGCTGGTCCCTTGCTGATTATTTTGGGCGTCCAAACCCTTTGTGCCGGGCTATTTGCCTAT
TTTGTGGTGTTTTGGGCTGCAGGTCATGATTATGATGCCAGCGTGATTTGTGCTGGTTTTACGGGAATGGTTCTAGGAGC
TATGCCCGTGGGCATGGCAAATATGACCACGATTACCCAGCGCTATGGACCTTCTCCCAAAGCCTTGCTGGTCATTCCTT
TATTGGGAGCTTGCTGCATGGATATTGCCAATGCGGTCGTTATTCAATTCTTTTTAATGCTGTCACCATAA

Upstream 100 bases:

>100_bases
GTCAATCAGGACAATATGACTTGACACGAGTCCAACCCGTCGACTTTTTTCCTCAAACGTCCCATGTGGAATGTGTCGCG
TTCCTCAATCGGCGGGCTTC

Downstream 100 bases:

>100_bases
AAACACCGCATTGCGCAAGAAATGTATCAAAAAATTACACAATATATATTTTGTTCAGGGGTCTCGTAACATTACATAAT
AATGGGGATCATTAACCTCG

Product: sodium/glutamate symporter

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MIETVVDIDCLTFLLISIFILGIGILLTRTLPLLRTLNIPAAVSGGLIGCGLVAYVNLCTPWHLRFDLSLRDNLLLIFFS
TIGLAARFKQLKEGGKTLAAVIGVMIAFLFLQNAVGIGVACLVGCPPIFGLLGGTIAFVGGHGTAITWGTLLEAQGWTGA
LEFSLAAATLGLIVGGVFAGPISQHLIRRFELESLSPPPDVVAESPVTMPTSLEGMFGIVMLIATSLSVGLGIHLFTLDW
GIVLPRFLPIMLVAILLINLLDWGNISINDTALSFWNDVSLQLFLVMSLMNLQILSWMQTAGPLLIILGVQTLCAGLFAY
FVVFWAAGHDYDASVICAGFTGMVLGAMPVGMANMTTITQRYGPSPKALLVIPLLGACCMDIANAVVIQFFLMLSP

Sequences:

>Translated_396_residues
MIETVVDIDCLTFLLISIFILGIGILLTRTLPLLRTLNIPAAVSGGLIGCGLVAYVNLCTPWHLRFDLSLRDNLLLIFFS
TIGLAARFKQLKEGGKTLAAVIGVMIAFLFLQNAVGIGVACLVGCPPIFGLLGGTIAFVGGHGTAITWGTLLEAQGWTGA
LEFSLAAATLGLIVGGVFAGPISQHLIRRFELESLSPPPDVVAESPVTMPTSLEGMFGIVMLIATSLSVGLGIHLFTLDW
GIVLPRFLPIMLVAILLINLLDWGNISINDTALSFWNDVSLQLFLVMSLMNLQILSWMQTAGPLLIILGVQTLCAGLFAY
FVVFWAAGHDYDASVICAGFTGMVLGAMPVGMANMTTITQRYGPSPKALLVIPLLGACCMDIANAVVIQFFLMLSP
>Mature_396_residues
MIETVVDIDCLTFLLISIFILGIGILLTRTLPLLRTLNIPAAVSGGLIGCGLVAYVNLCTPWHLRFDLSLRDNLLLIFFS
TIGLAARFKQLKEGGKTLAAVIGVMIAFLFLQNAVGIGVACLVGCPPIFGLLGGTIAFVGGHGTAITWGTLLEAQGWTGA
LEFSLAAATLGLIVGGVFAGPISQHLIRRFELESLSPPPDVVAESPVTMPTSLEGMFGIVMLIATSLSVGLGIHLFTLDW
GIVLPRFLPIMLVAILLINLLDWGNISINDTALSFWNDVSLQLFLVMSLMNLQILSWMQTAGPLLIILGVQTLCAGLFAY
FVVFWAAGHDYDASVICAGFTGMVLGAMPVGMANMTTITQRYGPSPKALLVIPLLGACCMDIANAVVIQFFLMLSP

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:
GO:0015501: Sodium/glutamate symporter
GO:0015813: Sodium/glutamate symporter
GO:0016021: Sodium/glutamate symporter

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=384, Percent_Identity=38.0208333333333, Blast_Score=214, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 42097; Mature: 42097

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETVVDIDCLTFLLISIFILGIGILLTRTLPLLRTLNIPAAVSGGLIGCGLVAYVNLCT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCC
PWHLRFDLSLRDNLLLIFFSTIGLAARFKQLKEGGKTLAAVIGVMIAFLFLQNAVGIGVA
CEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
CLVGCPPIFGLLGGTIAFVGGHGTAITWGTLLEAQGWTGALEFSLAAATLGLIVGGVFAG
HHHCCHHHHHHHCCCEEEECCCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PISQHLIRRFELESLSPPPDVVAESPVTMPTSLEGMFGIVMLIATSLSVGLGIHLFTLDW
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEHHH
GIVLPRFLPIMLVAILLINLLDWGNISINDTALSFWNDVSLQLFLVMSLMNLQILSWMQT
HHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AGPLLIILGVQTLCAGLFAYFVVFWAAGHDYDASVICAGFTGMVLGAMPVGMANMTTITQ
CCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHCCCCHHHHHHHH
RYGPSPKALLVIPLLGACCMDIANAVVIQFFLMLSP
HCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIETVVDIDCLTFLLISIFILGIGILLTRTLPLLRTLNIPAAVSGGLIGCGLVAYVNLCT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCC
PWHLRFDLSLRDNLLLIFFSTIGLAARFKQLKEGGKTLAAVIGVMIAFLFLQNAVGIGVA
CEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
CLVGCPPIFGLLGGTIAFVGGHGTAITWGTLLEAQGWTGALEFSLAAATLGLIVGGVFAG
HHHCCHHHHHHHCCCEEEECCCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PISQHLIRRFELESLSPPPDVVAESPVTMPTSLEGMFGIVMLIATSLSVGLGIHLFTLDW
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEHHH
GIVLPRFLPIMLVAILLINLLDWGNISINDTALSFWNDVSLQLFLVMSLMNLQILSWMQT
HHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AGPLLIILGVQTLCAGLFAYFVVFWAAGHDYDASVICAGFTGMVLGAMPVGMANMTTITQ
CCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHCCCCHHHHHHHH
RYGPSPKALLVIPLLGACCMDIANAVVIQFFLMLSP
HCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254324; 2017136; 7686882; 9278503; 1544582 [H]