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The map label for this gene is cynS

Identifier: 158338269

GI number: 158338269

Start: 5228550

End: 5228993

Strand: Reverse

Name: cynS

Synonym: AM1_5165

Alternate gene names: 158338269

Gene position: 5228993-5228550 (Counterclockwise)

Preceding gene: 158338270

Following gene: 158338264

Centisome position: 80.4

GC content: 47.52

Gene sequence:

>444_bases
ATGGCCATTGCTGAAATTACCGAGAAACTCCTAGCAGCAAAGAAAGCGAAAGGTATTTCCTTTGAAGATTTAGAAAAAAT
TGTCGGCTGTGATGAAACCTGGATTGCCTCTGTGATTTACCGCCAGGCGAGTGCCTCTACAGAAGAAGCCGAGAAAATTG
TTACAGCTTTGGGCTTACCCGCAGAGTTGGCGGAACCCCTAACCGTGCCGCCCATGAAAGGCTCTTTAGAACCCGTGATT
CCCACGGACCCCTTAGTCTACCGATTCTATGAAATTATGCAGGTCTATGGGGTGCCGGTTAAAGCCGTGATTCACGAGAA
ATTTGGCGACGGTATTATGAGTGCCATCGATTTCTCCATTGAGGTGGATAAAGTGCCAGATCCAAAAGGAGATCGCGTTC
AGGTAACCATGTGCGGTAAGTTCCTACCCTATAAGAAGTGGTAG

Upstream 100 bases:

>100_bases
AACGCGGTTTGTAGAACAGGACCGGATCAAGGTCAAAGCCTAACCCCCCTGAATATTCACTTTTCGACTGCAAATTTCTT
ATTTTGGAGAACCCATTCCT

Downstream 100 bases:

>100_bases
CCCCCTAGATATCCAATCGCTGATAAACCTGATCGAGGTTTTGTAAGTGGCGACTGGGGTCAAAGCAGTGGTCTAGTTCC
TCAGCCGAGAGCAGTTTTTG

Product: cyanate hydratase

Products: NA

Alternate protein names: Cyanase; Cyanate hydrolase; Cyanate lyase

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MAIAEITEKLLAAKKAKGISFEDLEKIVGCDETWIASVIYRQASASTEEAEKIVTALGLPAELAEPLTVPPMKGSLEPVI
PTDPLVYRFYEIMQVYGVPVKAVIHEKFGDGIMSAIDFSIEVDKVPDPKGDRVQVTMCGKFLPYKKW

Sequences:

>Translated_147_residues
MAIAEITEKLLAAKKAKGISFEDLEKIVGCDETWIASVIYRQASASTEEAEKIVTALGLPAELAEPLTVPPMKGSLEPVI
PTDPLVYRFYEIMQVYGVPVKAVIHEKFGDGIMSAIDFSIEVDKVPDPKGDRVQVTMCGKFLPYKKW
>Mature_146_residues
AIAEITEKLLAAKKAKGISFEDLEKIVGCDETWIASVIYRQASASTEEAEKIVTALGLPAELAEPLTVPPMKGSLEPVIP
TDPLVYRFYEIMQVYGVPVKAVIHEKFGDGIMSAIDFSIEVDKVPDPKGDRVQVTMCGKFLPYKKW

Specific function: Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide

COG id: COG1513

COG function: function code P; Cyanate lyase

Gene ontology:
GO:0003677: Cyanate hydratase
GO:0008152: Cyanate hydratase
GO:0008824: Cyanate hydratase
GO:0009439: Cyanate hydratase
GO:0016829: Cyanate hydratase
GO:0016836: Cyanate hydratase
GO:0043565: Cyanate hydratase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cyanase family

Homologues:

Organism=Escherichia coli, GI1786535, Length=144, Percent_Identity=38.1944444444444, Blast_Score=114, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYNS_ACAM1 (B0C8H2)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519446.1
- ProteinModelPortal:   B0C8H2
- SMR:   B0C8H2
- GeneID:   5683961
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5165
- HOGENOM:   HBG317643
- OMA:   KEADPKG
- ProtClustDB:   PRK02866
- BioCyc:   AMAR329726:AM1_5165-MONOMER
- HAMAP:   MF_00535
- InterPro:   IPR008076
- InterPro:   IPR003712
- InterPro:   IPR001387
- InterPro:   IPR010982
- Gene3D:   G3DSA:3.30.1160.10
- PIRSF:   PIRSF001263
- PRINTS:   PR01693
- SMART:   SM00530
- TIGRFAMs:   TIGR00673

Pfam domain/function: PF02560 Cyanate_lyase; SSF55234 Cyanate_lyase_C; SSF47413 Lambda_like_DNA

EC number: =4.2.1.104

Molecular weight: Translated: 16202; Mature: 16071

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: ACT_SITE 88-88 ACT_SITE 91-91 ACT_SITE 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAEITEKLLAAKKAKGISFEDLEKIVGCDETWIASVIYRQASASTEEAEKIVTALGLP
CCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
AELAEPLTVPPMKGSLEPVIPTDPLVYRFYEIMQVYGVPVKAVIHEKFGDGIMSAIDFSI
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCEE
EVDKVPDPKGDRVQVTMCGKFLPYKKW
EECCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
AIAEITEKLLAAKKAKGISFEDLEKIVGCDETWIASVIYRQASASTEEAEKIVTALGLP
CHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
AELAEPLTVPPMKGSLEPVIPTDPLVYRFYEIMQVYGVPVKAVIHEKFGDGIMSAIDFSI
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCEE
EVDKVPDPKGDRVQVTMCGKFLPYKKW
EECCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA