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The map label for this gene is ldhA [H]

Identifier: 158338227

GI number: 158338227

Start: 5182141

End: 5183133

Strand: Direct

Name: ldhA [H]

Synonym: AM1_5122

Alternate gene names: 158338227

Gene position: 5182141-5183133 (Clockwise)

Preceding gene: 158338225

Following gene: 158338228

Centisome position: 79.68

GC content: 46.63

Gene sequence:

>993_bases
ATGAAAGTTGCTATTTTTAGTAGTAAACAGTACGACCAGCAATTTCTGCGGGCTGCCAATCGGGAAGGCCATGAACTTGT
TTTTTATGAGCCTCGCTTAACGGCTACTACAGCTCCGCTTGCCAAGGATTTTCCTATTGTTTGCGTCTTTATTAACGATC
AGTTGGATGCGGAAACCCTGGAGATTTTGGCAGATCAGGGGACCCAATTGGTGGCCCTGCGATCGGCAGGCTTTAATAAT
GTCGATGTAGCTAAGGCAACTGAACTGGGCATCACGGTTGTTCGGGTGCCTGCCTATTCGCCCTATTCCGTGGCAGAGCA
TGCTGTAGGCATGATTTTGATGCTCAATCGCAAGCTGTATAGAGCCTATAACCGAGTTCGGGATGATAATTTCTCCCTGG
AAGGGCTACTCGGGTTTGATCTCCATAACTGTTCAGTGGGAATTGTAGGAACGGGCAAAATAGGTCAATGTTTTGCCCAA
ATTATGAAAGGTTTTGGCTGCCAGCTATTGGCCTATGATCCCTATCCTAACCCCGCCTGTATTGAGCTGGGTGTGACTTA
TATGGCACTGGAAGACTTGCTGGCTCAATCCAAGATTGTTTCTTTGCATTGCCCGTTGATGCCCCAAACCCATCACATCA
TTAATGCCGCGACCTTAAAGGTATTGCAACCTGGCACGATGTTGATTAATACCAGTCGGGGTGGGCTGATTGATACCCCT
GCTGTGATTGATGCGATTAAATCGGGGCAAGTGGGATATTTAGGCATTGATGTCTACGAACAAGAGGAAAACCTATTTTT
CGAAGATCTGTCTGATACGGTGATTCAGGACGATCACTTTCAGCTCTTACAATCCTTCCCTAACGTTTTAATTACGGCCC
ATCAGGCATTTTTCACTCGCAATGCCCTGGAGAATATCGCCAGCACTACCTTGAGCAATATTGGTGATTTTGCAGCTGGG
CGTCCCTGCATTAATCAAGTCAAATTGACCTAG

Upstream 100 bases:

>100_bases
CGATCCTAGCTGGCCTTAACCTGGTGGGGCTTTTTAGTTGCTAAGTTACAAGCAGAGACAACGGTTGGAGCAGGGTAGTG
TCTCTGAGGAAGAAAAAAAA

Downstream 100 bases:

>100_bases
TCCGGGGTATCCTAATCAACGGTTACTTCTATATTTCAGTGGCTATCAACTAGCGCTATCTAGATTTCATTCACTCTCAT
TAGTGCCAATATTCATGAGT

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKVAIFSSKQYDQQFLRAANREGHELVFYEPRLTATTAPLAKDFPIVCVFINDQLDAETLEILADQGTQLVALRSAGFNN
VDVAKATELGITVVRVPAYSPYSVAEHAVGMILMLNRKLYRAYNRVRDDNFSLEGLLGFDLHNCSVGIVGTGKIGQCFAQ
IMKGFGCQLLAYDPYPNPACIELGVTYMALEDLLAQSKIVSLHCPLMPQTHHIINAATLKVLQPGTMLINTSRGGLIDTP
AVIDAIKSGQVGYLGIDVYEQEENLFFEDLSDTVIQDDHFQLLQSFPNVLITAHQAFFTRNALENIASTTLSNIGDFAAG
RPCINQVKLT

Sequences:

>Translated_330_residues
MKVAIFSSKQYDQQFLRAANREGHELVFYEPRLTATTAPLAKDFPIVCVFINDQLDAETLEILADQGTQLVALRSAGFNN
VDVAKATELGITVVRVPAYSPYSVAEHAVGMILMLNRKLYRAYNRVRDDNFSLEGLLGFDLHNCSVGIVGTGKIGQCFAQ
IMKGFGCQLLAYDPYPNPACIELGVTYMALEDLLAQSKIVSLHCPLMPQTHHIINAATLKVLQPGTMLINTSRGGLIDTP
AVIDAIKSGQVGYLGIDVYEQEENLFFEDLSDTVIQDDHFQLLQSFPNVLITAHQAFFTRNALENIASTTLSNIGDFAAG
RPCINQVKLT
>Mature_330_residues
MKVAIFSSKQYDQQFLRAANREGHELVFYEPRLTATTAPLAKDFPIVCVFINDQLDAETLEILADQGTQLVALRSAGFNN
VDVAKATELGITVVRVPAYSPYSVAEHAVGMILMLNRKLYRAYNRVRDDNFSLEGLLGFDLHNCSVGIVGTGKIGQCFAQ
IMKGFGCQLLAYDPYPNPACIELGVTYMALEDLLAQSKIVSLHCPLMPQTHHIINAATLKVLQPGTMLINTSRGGLIDTP
AVIDAIKSGQVGYLGIDVYEQEENLFFEDLSDTVIQDDHFQLLQSFPNVLITAHQAFFTRNALENIASTTLSNIGDFAAG
RPCINQVKLT

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=270, Percent_Identity=31.8518518518519, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4557497, Length=270, Percent_Identity=31.8518518518519, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI23308577, Length=207, Percent_Identity=31.8840579710145, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI145580578, Length=256, Percent_Identity=32.421875, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4557499, Length=256, Percent_Identity=32.421875, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI145580575, Length=256, Percent_Identity=32.421875, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI6912396, Length=219, Percent_Identity=27.8538812785388, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=50.9146341463415, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI1789279, Length=231, Percent_Identity=31.6017316017316, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI87082289, Length=194, Percent_Identity=30.4123711340206, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788660, Length=258, Percent_Identity=27.1317829457364, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=265, Percent_Identity=27.1698113207547, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25147481, Length=266, Percent_Identity=28.1954887218045, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=202, Percent_Identity=33.6633663366337, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=238, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=238, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324964, Length=268, Percent_Identity=27.2388059701493, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24646446, Length=290, Percent_Identity=35.5172413793103, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24646448, Length=290, Percent_Identity=35.5172413793103, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24646452, Length=290, Percent_Identity=35.5172413793103, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24646450, Length=290, Percent_Identity=35.5172413793103, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI62472511, Length=290, Percent_Identity=35.5172413793103, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19921140, Length=268, Percent_Identity=28.7313432835821, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28574286, Length=274, Percent_Identity=27.3722627737226, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45552429, Length=280, Percent_Identity=24.6428571428571, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585514, Length=280, Percent_Identity=24.6428571428571, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574282, Length=280, Percent_Identity=24.6428571428571, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574284, Length=280, Percent_Identity=24.6428571428571, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45551003, Length=280, Percent_Identity=24.6428571428571, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28571528, Length=285, Percent_Identity=26.6666666666667, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585516, Length=207, Percent_Identity=22.7053140096618, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36263; Mature: 36263

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAIFSSKQYDQQFLRAANREGHELVFYEPRLTATTAPLAKDFPIVCVFINDQLDAETL
CEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
EILADQGTQLVALRSAGFNNVDVAKATELGITVVRVPAYSPYSVAEHAVGMILMLNRKLY
HHHHCCCCEEEEEECCCCCCCCCCCHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
RAYNRVRDDNFSLEGLLGFDLHNCSVGIVGTGKIGQCFAQIMKGFGCQLLAYDPYPNPAC
HHHHHHCCCCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCE
IELGVTYMALEDLLAQSKIVSLHCPLMPQTHHIINAATLKVLQPGTMLINTSRGGLIDTP
EEHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHEEEEECCCEEEEECCCCCCCCCH
AVIDAIKSGQVGYLGIDVYEQEENLFFEDLSDTVIQDDHFQLLQSFPNVLITAHQAFFTR
HHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHCCHHEEEHHHHHHHH
NALENIASTTLSNIGDFAAGRPCINQVKLT
HHHHHHHHHHHHHHHHHHCCCCCCCCEECC
>Mature Secondary Structure
MKVAIFSSKQYDQQFLRAANREGHELVFYEPRLTATTAPLAKDFPIVCVFINDQLDAETL
CEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
EILADQGTQLVALRSAGFNNVDVAKATELGITVVRVPAYSPYSVAEHAVGMILMLNRKLY
HHHHCCCCEEEEEECCCCCCCCCCCHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
RAYNRVRDDNFSLEGLLGFDLHNCSVGIVGTGKIGQCFAQIMKGFGCQLLAYDPYPNPAC
HHHHHHCCCCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCE
IELGVTYMALEDLLAQSKIVSLHCPLMPQTHHIINAATLKVLQPGTMLINTSRGGLIDTP
EEHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHEEEEECCCEEEEECCCCCCCCCH
AVIDAIKSGQVGYLGIDVYEQEENLFFEDLSDTVIQDDHFQLLQSFPNVLITAHQAFFTR
HHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHCCHHEEEHHHHHHHH
NALENIASTTLSNIGDFAAGRPCINQVKLT
HHHHHHHHHHHHHHHHHHCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]