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The map label for this gene is pilG [H]

Identifier: 158338187

GI number: 158338187

Start: 5133419

End: 5134576

Strand: Reverse

Name: pilG [H]

Synonym: AM1_5080

Alternate gene names: 158338187

Gene position: 5134576-5133419 (Counterclockwise)

Preceding gene: 158338188

Following gene: 158338186

Centisome position: 78.95

GC content: 43.18

Gene sequence:

>1158_bases
ATGCTTCAGTTCCAAGCTGGACAATTACAGCAATCTTTAGAAGAACTCCGACAAGACGCTTTTAGTGGAGCCGCCTATAT
TGATGTTTCTATCAATACCCAAAATATAGTCCGCTCACAAGTCGCTTTCTTCAAAGAAGGAGCGATTACTTATATCGGTA
ATGACTTGCCTAATCCCATAGACTTCTCACAAGAAATTGGGAAACTGTTGAATCTGAAAATTATCAATGCTGCCATCAAA
ATGGCAGAGAAAAAGGTAAAGGATCCAACCTCCATTCATTCTTATTTATCTATTTATACTCGCATCAATCTCTTTACCTG
GGAGCAGATCGAGAAATTGATATTTAACCAAACGGTCTTGAGGTTAGACCAGCTTTTACCTTATGAAGGCCGACTGGTAA
CCAATGCTCAAGCAGAGTTTGATCTTTGTTATGGAGAAGATCGGCATGGACTAGATTGGCAACAACTGCAAGCAACATTA
GTGAAGCGTCAACAGGCCTGGACTGACCTTGAACCGGATATTCCGTCCATTGATGCCATTCCCCATCCCATCCAAATCAA
CAAAAATCATGCAGTTGATGATGCTCATGCACAGAAACATTTACAGACATGGATAGACGGTCAGAGATCTTTGTTGCAAA
TTGCATCTGAAATGAAGCAAGACCCGTTGGCATTGGGACAGCTTTATCTCCGTTGGCACAAGCTAGGGTGGATAACCTTC
ACGGGAGCTCAGCCTAAAATTACGGCTCAACCATCGACAACAGAACAAAAAGGCTTGCCAGTAATATTAAGTGTGGACGA
CAGCCTCGTTGTCCAAACCATGATTAAACGGGCCATCGGAGATCACTATAAAGTCTTGACCGCCAATAGTGCGATAGATG
CCCTACAGGTGCTGAACAACAATTCAAATTCAGTGGAGCTGTTGCTGTTAGACGTCACGATGCCTGATGTTGATGGTTTT
GAACTCTGTCGCACGGTGAGATCTATCCCACAGTTCCAAAACCTACCTGTAATTATGCTGACGGCCAAAGACGGCCTTTT
AAATAAGGTAAAGGGACAAATGGCGGGATCAACCCACTATTTAACCAAACCCGTTGAAAAAGAGACATTACTCAACGCCA
TCGAAAAGTACATTCCTTCCCCCCAAATGAGTCGCTAA

Upstream 100 bases:

>100_bases
TCTATATACCTGAGCTTTTTTATCGCGGCACTAGAATACTGGGATTGTTTTTTTCTCCTGTATAAAAAGAAAAAATGATG
CTTCATCTGGATGGTCAATA

Downstream 100 bases:

>100_bases
TGGGTTCAATATTTTTCCAAAGGACATATGGTTATGGACAATCAAGCTTATCTGATCTTTCAGCTGCAAGAATTGTGGTA
TGGAATTGATGCTGAGCTCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MLQFQAGQLQQSLEELRQDAFSGAAYIDVSINTQNIVRSQVAFFKEGAITYIGNDLPNPIDFSQEIGKLLNLKIINAAIK
MAEKKVKDPTSIHSYLSIYTRINLFTWEQIEKLIFNQTVLRLDQLLPYEGRLVTNAQAEFDLCYGEDRHGLDWQQLQATL
VKRQQAWTDLEPDIPSIDAIPHPIQINKNHAVDDAHAQKHLQTWIDGQRSLLQIASEMKQDPLALGQLYLRWHKLGWITF
TGAQPKITAQPSTTEQKGLPVILSVDDSLVVQTMIKRAIGDHYKVLTANSAIDALQVLNNNSNSVELLLLDVTMPDVDGF
ELCRTVRSIPQFQNLPVIMLTAKDGLLNKVKGQMAGSTHYLTKPVEKETLLNAIEKYIPSPQMSR

Sequences:

>Translated_385_residues
MLQFQAGQLQQSLEELRQDAFSGAAYIDVSINTQNIVRSQVAFFKEGAITYIGNDLPNPIDFSQEIGKLLNLKIINAAIK
MAEKKVKDPTSIHSYLSIYTRINLFTWEQIEKLIFNQTVLRLDQLLPYEGRLVTNAQAEFDLCYGEDRHGLDWQQLQATL
VKRQQAWTDLEPDIPSIDAIPHPIQINKNHAVDDAHAQKHLQTWIDGQRSLLQIASEMKQDPLALGQLYLRWHKLGWITF
TGAQPKITAQPSTTEQKGLPVILSVDDSLVVQTMIKRAIGDHYKVLTANSAIDALQVLNNNSNSVELLLLDVTMPDVDGF
ELCRTVRSIPQFQNLPVIMLTAKDGLLNKVKGQMAGSTHYLTKPVEKETLLNAIEKYIPSPQMSR
>Mature_385_residues
MLQFQAGQLQQSLEELRQDAFSGAAYIDVSINTQNIVRSQVAFFKEGAITYIGNDLPNPIDFSQEIGKLLNLKIINAAIK
MAEKKVKDPTSIHSYLSIYTRINLFTWEQIEKLIFNQTVLRLDQLLPYEGRLVTNAQAEFDLCYGEDRHGLDWQQLQATL
VKRQQAWTDLEPDIPSIDAIPHPIQINKNHAVDDAHAQKHLQTWIDGQRSLLQIASEMKQDPLALGQLYLRWHKLGWITF
TGAQPKITAQPSTTEQKGLPVILSVDDSLVVQTMIKRAIGDHYKVLTANSAIDALQVLNNNSNSVELLLLDVTMPDVDGF
ELCRTVRSIPQFQNLPVIMLTAKDGLLNKVKGQMAGSTHYLTKPVEKETLLNAIEKYIPSPQMSR

Specific function: Is involved in pilus biosynthesis and twitching motility. May act as a single-domain response regulator whose function may be to receive a certain environmental signal(s) and then transduce that signal to the pilus assembly machinery via specific protein-

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=138, Percent_Identity=31.8840579710145, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 43406; Mature: 43406

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQFQAGQLQQSLEELRQDAFSGAAYIDVSINTQNIVRSQVAFFKEGAITYIGNDLPNPI
CCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHCCCEEEECCCCCCCC
DFSQEIGKLLNLKIINAAIKMAEKKVKDPTSIHSYLSIYTRINLFTWEQIEKLIFNQTVL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHH
RLDQLLPYEGRLVTNAQAEFDLCYGEDRHGLDWQQLQATLVKRQQAWTDLEPDIPSIDAI
HHHHHCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PHPIQINKNHAVDDAHAQKHLQTWIDGQRSLLQIASEMKQDPLALGQLYLRWHKLGWITF
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEEEEE
TGAQPKITAQPSTTEQKGLPVILSVDDSLVVQTMIKRAIGDHYKVLTANSAIDALQVLNN
ECCCCEEECCCCCCHHCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCC
NSNSVELLLLDVTMPDVDGFELCRTVRSIPQFQNLPVIMLTAKDGLLNKVKGQMAGSTHY
CCCCEEEEEEEEECCCCCHHHHHHHHHHCHHHCCCCEEEEECCCCHHHHHHHHHCCCCCH
LTKPVEKETLLNAIEKYIPSPQMSR
HCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLQFQAGQLQQSLEELRQDAFSGAAYIDVSINTQNIVRSQVAFFKEGAITYIGNDLPNPI
CCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHCCCEEEECCCCCCCC
DFSQEIGKLLNLKIINAAIKMAEKKVKDPTSIHSYLSIYTRINLFTWEQIEKLIFNQTVL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHH
RLDQLLPYEGRLVTNAQAEFDLCYGEDRHGLDWQQLQATLVKRQQAWTDLEPDIPSIDAI
HHHHHCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PHPIQINKNHAVDDAHAQKHLQTWIDGQRSLLQIASEMKQDPLALGQLYLRWHKLGWITF
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEEEEE
TGAQPKITAQPSTTEQKGLPVILSVDDSLVVQTMIKRAIGDHYKVLTANSAIDALQVLNN
ECCCCEEECCCCCCHHCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCC
NSNSVELLLLDVTMPDVDGFELCRTVRSIPQFQNLPVIMLTAKDGLLNKVKGQMAGSTHY
CCCCEEEEEEEEECCCCCHHHHHHHHHHCHHHCCCCEEEEECCCCHHHHHHHHHCCCCCH
LTKPVEKETLLNAIEKYIPSPQMSR
HCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8104179; 10984043 [H]