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The map label for this gene is pilG [H]

Identifier: 158338188

GI number: 158338188

Start: 5134940

End: 5136106

Strand: Reverse

Name: pilG [H]

Synonym: AM1_5081

Alternate gene names: 158338188

Gene position: 5136106-5134940 (Counterclockwise)

Preceding gene: 158338194

Following gene: 158338187

Centisome position: 78.97

GC content: 46.36

Gene sequence:

>1167_bases
ATGCTTAAGTTCGAGGCAGGAAAATTACAGAAAACGCTAGGCCAACTACACCAGGAAGAGTTCTCAGGTGTCGTCTATAT
CAATGCAGCCCTTGGCCAACAGGACAAAGTCAGATCACGGGTTATTGCCTTTCATAAAGGGGCAATTACCTACGCAGGTA
AAACCTTACCTACGCCTCTTGAGTTTTCCCAACTGGTTGGCGAAAAGCTGAAGCTTAAAGTCATGAAGGCGGCCATGCTG
TTAGCAGAAAAAAGGGCCAAAGATTCCACTTCAATTCTGGCTTACTTAGCCATTTACTTTCGCCTCGATCTGTTTGGCTG
GGACGCCTTGGAAAAGGTGATGCGATCGCAAGTGGTATTTCATCTCGATCAACTCTGGTCCTATGCCGGTACCCTGAGAA
CGGAAGAGACCGTTGAGTTTGATCTGGCCTATGGTGAGGATGGCCACGGTTTTGATTGGCAGCAGCTCTGTTTGTCCTTA
ACCCAGCGCCAACAAGCATGGGCCTCTCTGACTCCTGCAATCCCGTCTATGGACGCGATCCCTCAGCCCATTGTGCAAGG
GAATTATGAAGCGGTAGAGGATGAGTATGCTAGACAGCATCTGACAATATGGATTGATGGCAACCGATCCTTACTGGATA
TTGCCGAGGGGTTACAGCGTGATCCACTGGAATTGGGCCATCTTTATTTACATTGGCAAAAGATGAATTGGGTAATCTTC
GCTGGTGATCAGCTCCCATATTTAGGCGGAAACCCCATTACAACTGCTCAACAGAGCTTACCCTTAATTTTGAGCGTGGA
TGATAGCGTCGTTGTCCAAACCATGCTTAAACGAACGTTATGTGAATACTATCGGTTCTTGACCGCTAGTAACGCTGTTG
AAGCTCTCAATCTTCTAAATCGCTATCCCATAGAGTTGATGCTATTGGATGTGACAATGCCCGATATCGATGGCATCGAG
TTCTGTAAAACGGTTCGAGGCATTTCTAAGTTCAGAGATTTGCCGGTGATTATGCTGACGGCCAAGGACGGATTGATTGA
TAAGGTTAAGGGCCAGATGGCTGGATCAACCCACTATCTAACCAAACCTGTCAGAAAAGAAAAGTTACTCAATGTAATAG
AAAAGAACCTGCCGTCCAGTAAAGTGATGAGTTGGAGACTTCATTAA

Upstream 100 bases:

>100_bases
GGTGGGCTTTATCTAGATTTTTAACTAGTAAGCTGAGTATTCAAAAGTCGCCCTTTACAGACTTCTAAAGTTGGCACTTT
TCTCTTGGGGGAGGAATGAA

Downstream 100 bases:

>100_bases
CCGCTGGTTATGATCCCCCACCCAGCCCTAAAGCCTGACCATAAGGAGATTTCAGCCAATTGAAACACTCATAACAAGAA
ATTAATTTTTCCTTAAGGAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MLKFEAGKLQKTLGQLHQEEFSGVVYINAALGQQDKVRSRVIAFHKGAITYAGKTLPTPLEFSQLVGEKLKLKVMKAAML
LAEKRAKDSTSILAYLAIYFRLDLFGWDALEKVMRSQVVFHLDQLWSYAGTLRTEETVEFDLAYGEDGHGFDWQQLCLSL
TQRQQAWASLTPAIPSMDAIPQPIVQGNYEAVEDEYARQHLTIWIDGNRSLLDIAEGLQRDPLELGHLYLHWQKMNWVIF
AGDQLPYLGGNPITTAQQSLPLILSVDDSVVVQTMLKRTLCEYYRFLTASNAVEALNLLNRYPIELMLLDVTMPDIDGIE
FCKTVRGISKFRDLPVIMLTAKDGLIDKVKGQMAGSTHYLTKPVRKEKLLNVIEKNLPSSKVMSWRLH

Sequences:

>Translated_388_residues
MLKFEAGKLQKTLGQLHQEEFSGVVYINAALGQQDKVRSRVIAFHKGAITYAGKTLPTPLEFSQLVGEKLKLKVMKAAML
LAEKRAKDSTSILAYLAIYFRLDLFGWDALEKVMRSQVVFHLDQLWSYAGTLRTEETVEFDLAYGEDGHGFDWQQLCLSL
TQRQQAWASLTPAIPSMDAIPQPIVQGNYEAVEDEYARQHLTIWIDGNRSLLDIAEGLQRDPLELGHLYLHWQKMNWVIF
AGDQLPYLGGNPITTAQQSLPLILSVDDSVVVQTMLKRTLCEYYRFLTASNAVEALNLLNRYPIELMLLDVTMPDIDGIE
FCKTVRGISKFRDLPVIMLTAKDGLIDKVKGQMAGSTHYLTKPVRKEKLLNVIEKNLPSSKVMSWRLH
>Mature_388_residues
MLKFEAGKLQKTLGQLHQEEFSGVVYINAALGQQDKVRSRVIAFHKGAITYAGKTLPTPLEFSQLVGEKLKLKVMKAAML
LAEKRAKDSTSILAYLAIYFRLDLFGWDALEKVMRSQVVFHLDQLWSYAGTLRTEETVEFDLAYGEDGHGFDWQQLCLSL
TQRQQAWASLTPAIPSMDAIPQPIVQGNYEAVEDEYARQHLTIWIDGNRSLLDIAEGLQRDPLELGHLYLHWQKMNWVIF
AGDQLPYLGGNPITTAQQSLPLILSVDDSVVVQTMLKRTLCEYYRFLTASNAVEALNLLNRYPIELMLLDVTMPDIDGIE
FCKTVRGISKFRDLPVIMLTAKDGLIDKVKGQMAGSTHYLTKPVRKEKLLNVIEKNLPSSKVMSWRLH

Specific function: Is involved in pilus biosynthesis and twitching motility. May act as a single-domain response regulator whose function may be to receive a certain environmental signal(s) and then transduce that signal to the pilus assembly machinery via specific protein-

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=112, Percent_Identity=34.8214285714286, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788905, Length=128, Percent_Identity=31.25, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 43975; Mature: 43975

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFEAGKLQKTLGQLHQEEFSGVVYINAALGQQDKVRSRVIAFHKGAITYAGKTLPTPL
CCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCH
EFSQLVGEKLKLKVMKAAMLLAEKRAKDSTSILAYLAIYFRLDLFGWDALEKVMRSQVVF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
HLDQLWSYAGTLRTEETVEFDLAYGEDGHGFDWQQLCLSLTQRQQAWASLTPAIPSMDAI
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PQPIVQGNYEAVEDEYARQHLTIWIDGNRSLLDIAEGLQRDPLELGHLYLHWQKMNWVIF
CCHHHCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCHHHCEEEEEEEEEEEEEE
AGDQLPYLGGNPITTAQQSLPLILSVDDSVVVQTMLKRTLCEYYRFLTASNAVEALNLLN
ECCCCCCCCCCCCCCHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYPIELMLLDVTMPDIDGIEFCKTVRGISKFRDLPVIMLTAKDGLIDKVKGQMAGSTHYL
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCHHH
TKPVRKEKLLNVIEKNLPSSKVMSWRLH
CCHHHHHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MLKFEAGKLQKTLGQLHQEEFSGVVYINAALGQQDKVRSRVIAFHKGAITYAGKTLPTPL
CCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCH
EFSQLVGEKLKLKVMKAAMLLAEKRAKDSTSILAYLAIYFRLDLFGWDALEKVMRSQVVF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
HLDQLWSYAGTLRTEETVEFDLAYGEDGHGFDWQQLCLSLTQRQQAWASLTPAIPSMDAI
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PQPIVQGNYEAVEDEYARQHLTIWIDGNRSLLDIAEGLQRDPLELGHLYLHWQKMNWVIF
CCHHHCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCHHHCEEEEEEEEEEEEEE
AGDQLPYLGGNPITTAQQSLPLILSVDDSVVVQTMLKRTLCEYYRFLTASNAVEALNLLN
ECCCCCCCCCCCCCCHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYPIELMLLDVTMPDIDGIEFCKTVRGISKFRDLPVIMLTAKDGLIDKVKGQMAGSTHYL
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCHHH
TKPVRKEKLLNVIEKNLPSSKVMSWRLH
CCHHHHHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8104179; 10984043 [H]