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The map label for this gene is barA [H]

Identifier: 158338166

GI number: 158338166

Start: 5104030

End: 5105097

Strand: Direct

Name: barA [H]

Synonym: AM1_5058

Alternate gene names: 158338166

Gene position: 5104030-5105097 (Clockwise)

Preceding gene: 158338165

Following gene: 158338167

Centisome position: 78.48

GC content: 50.94

Gene sequence:

>1068_bases
ATGTCGCCTGCTTTATTGTCTAACCGCTATATTCTGGCCGTTGACGATCTGCCAGATAATTTGATGCTGCTCAAAATGAT
GTTGGAGAGTGCGGGCTACCGAATAGAGCTGGTGGATAGTGGTCCGGCGGCGCTGGCACAGATCCAAAAAGCCCAACCCG
ATTTAGTGATTTTGGATGTGATGATGCCGGGGATGAGTGGCTATGAAGTCACCCAGAAGATTCGCAATGACCCTCAATTA
CCCTATATTCCTATTCTGTTGATATCCGCCCACGAGCGCTCAAGCGTGGTCAAGGGACTGGATGCCGGAGCCGATGATTT
TATTCGTAAACCTGTGGAGTTAGAGGAACTGCAGGCGCGGGTGCGATCGCTTCTGCGTCTCAAGCAATCCATCGACCAGC
GGGAGAACTTTATCTCTTGCCTCACCCATGATTTACGGACGCCGTTAATCGCTTGCGATCGCATGGTGGACTTGGTCCGC
CAAGGGGTATTTGGAGACATCGCCCCAGCGGTTGAGGAAGCCCTGGCGGGCGTTAGCAGCAGCAACCAAAACTTGCTGCA
GATGCTCAACAATTTGCTAGAGGTCTACTGCTATGAACTGGGGGAAAAGAAACTCAGTTTTATCTCCGTTGATATGCAGG
AATTGATCACTGAGGTGGTTACAGAACTCCATCCCCTGGCCTTAGAAAAGAACCTAGAGCTAATCGGCAATTGGCAGTCT
GAGGTGCGGGAATTGAGTTGCGATCGCATGGAGTTGCGCCGGGTAATCACCAACCTCATCAACAATGCCATAAAGTTTAC
GGACACGGGTTCTGTAACCGTATCCAGTACCTCCGACACGAAACACCTTCAAATTGAGATTCAAGATACCGGCGTTGGTA
TTGCCGAAGCGGACCAAGCCCAGATTTTTGAGAGGTTTAAACAGACCCAGCATAATCGCTCTGGCCACGGCCTAGGGTTG
CATTTATGTCAGCAAGTGGTCCAGGCTCATGGCGGGGAATTATCGGTGAGATCGCAACTTGGTCAAGGGAGTACCTTCAC
CCTTCAGCTCCCTCTGGCTGACTCTTAA

Upstream 100 bases:

>100_bases
GTTGGCTCAACTTTTACCGTTGAATTGCCCCTACAAGTGCAGACCTTGCCGAATGCCAAAGCTAAATCCTCTGTAATCTA
GTGCCCCCTTTGAGAAGTTT

Downstream 100 bases:

>100_bases
TTGCTGGCCAACATTCCCTGCAAAAAGGATCTATGTCTAAAGGTATAGATCTGTGTATACCTACGGGTAGAACCCTTTCC
CAGCTTGGGTTGATAGGGTA

Product: two-component hybrid histidine kinase sensor and regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSPALLSNRYILAVDDLPDNLMLLKMMLESAGYRIELVDSGPAALAQIQKAQPDLVILDVMMPGMSGYEVTQKIRNDPQL
PYIPILLISAHERSSVVKGLDAGADDFIRKPVELEELQARVRSLLRLKQSIDQRENFISCLTHDLRTPLIACDRMVDLVR
QGVFGDIAPAVEEALAGVSSSNQNLLQMLNNLLEVYCYELGEKKLSFISVDMQELITEVVTELHPLALEKNLELIGNWQS
EVRELSCDRMELRRVITNLINNAIKFTDTGSVTVSSTSDTKHLQIEIQDTGVGIAEADQAQIFERFKQTQHNRSGHGLGL
HLCQQVVQAHGGELSVRSQLGQGSTFTLQLPLADS

Sequences:

>Translated_355_residues
MSPALLSNRYILAVDDLPDNLMLLKMMLESAGYRIELVDSGPAALAQIQKAQPDLVILDVMMPGMSGYEVTQKIRNDPQL
PYIPILLISAHERSSVVKGLDAGADDFIRKPVELEELQARVRSLLRLKQSIDQRENFISCLTHDLRTPLIACDRMVDLVR
QGVFGDIAPAVEEALAGVSSSNQNLLQMLNNLLEVYCYELGEKKLSFISVDMQELITEVVTELHPLALEKNLELIGNWQS
EVRELSCDRMELRRVITNLINNAIKFTDTGSVTVSSTSDTKHLQIEIQDTGVGIAEADQAQIFERFKQTQHNRSGHGLGL
HLCQQVVQAHGGELSVRSQLGQGSTFTLQLPLADS
>Mature_354_residues
SPALLSNRYILAVDDLPDNLMLLKMMLESAGYRIELVDSGPAALAQIQKAQPDLVILDVMMPGMSGYEVTQKIRNDPQLP
YIPILLISAHERSSVVKGLDAGADDFIRKPVELEELQARVRSLLRLKQSIDQRENFISCLTHDLRTPLIACDRMVDLVRQ
GVFGDIAPAVEEALAGVSSSNQNLLQMLNNLLEVYCYELGEKKLSFISVDMQELITEVVTELHPLALEKNLELIGNWQSE
VRELSCDRMELRRVITNLINNAIKFTDTGSVTVSSTSDTKHLQIEIQDTGVGIAEADQAQIFERFKQTQHNRSGHGLGLH
LCQQVVQAHGGELSVRSQLGQGSTFTLQLPLADS

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=251, Percent_Identity=33.4661354581673, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI145693157, Length=251, Percent_Identity=31.0756972111554, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87081816, Length=264, Percent_Identity=31.0606060606061, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=28.1938325991189, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI48994928, Length=244, Percent_Identity=27.8688524590164, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082128, Length=249, Percent_Identity=27.710843373494, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788549, Length=246, Percent_Identity=27.6422764227642, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788393, Length=261, Percent_Identity=24.5210727969349, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=222, Percent_Identity=24.7747747747748, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786783, Length=255, Percent_Identity=25.0980392156863, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790436, Length=242, Percent_Identity=28.5123966942149, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788394, Length=190, Percent_Identity=32.6315789473684, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786600, Length=250, Percent_Identity=26, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082012, Length=102, Percent_Identity=42.156862745098, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790861, Length=252, Percent_Identity=25.7936507936508, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=32.7731092436975, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786784, Length=137, Percent_Identity=34.3065693430657, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790860, Length=104, Percent_Identity=37.5, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790552, Length=136, Percent_Identity=34.5588235294118, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790437, Length=123, Percent_Identity=34.9593495934959, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=35.8974358974359, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790346, Length=257, Percent_Identity=24.5136186770428, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789808, Length=266, Percent_Identity=25.187969924812, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787229, Length=116, Percent_Identity=31.8965517241379, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39398; Mature: 39267

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPALLSNRYILAVDDLPDNLMLLKMMLESAGYRIELVDSGPAALAQIQKAQPDLVILDV
CCCCEECCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCEEEEEE
MMPGMSGYEVTQKIRNDPQLPYIPILLISAHERSSVVKGLDAGADDFIRKPVELEELQAR
CCCCCCCHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHCCCCCHHHHHCCCCHHHHHHH
VRSLLRLKQSIDQRENFISCLTHDLRTPLIACDRMVDLVRQGVFGDIAPAVEEALAGVSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
SNQNLLQMLNNLLEVYCYELGEKKLSFISVDMQELITEVVTELHPLALEKNLELIGNWQS
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHH
EVRELSCDRMELRRVITNLINNAIKFTDTGSVTVSSTSDTKHLQIEIQDTGVGIAEADQA
HHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCEEEEEEEEECCCCCCCCHHH
QIFERFKQTQHNRSGHGLGLHLCQQVVQAHGGELSVRSQLGQGSTFTLQLPLADS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEHHHHCCCCCEEEEEECCCCC
>Mature Secondary Structure 
SPALLSNRYILAVDDLPDNLMLLKMMLESAGYRIELVDSGPAALAQIQKAQPDLVILDV
CCCEECCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCEEEEEE
MMPGMSGYEVTQKIRNDPQLPYIPILLISAHERSSVVKGLDAGADDFIRKPVELEELQAR
CCCCCCCHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHCCCCCHHHHHCCCCHHHHHHH
VRSLLRLKQSIDQRENFISCLTHDLRTPLIACDRMVDLVRQGVFGDIAPAVEEALAGVSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
SNQNLLQMLNNLLEVYCYELGEKKLSFISVDMQELITEVVTELHPLALEKNLELIGNWQS
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHH
EVRELSCDRMELRRVITNLINNAIKFTDTGSVTVSSTSDTKHLQIEIQDTGVGIAEADQA
HHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCEEEEEEEEECCCCCCCCHHH
QIFERFKQTQHNRSGHGLGLHLCQQVVQAHGGELSVRSQLGQGSTFTLQLPLADS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEHHHHCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]