Click here to switch to the map view.

The map label for this gene is gacS [H]

Identifier: 158338165

GI number: 158338165

Start: 5102757

End: 5104010

Strand: Direct

Name: gacS [H]

Synonym: AM1_5057

Alternate gene names: 158338165

Gene position: 5102757-5104010 (Clockwise)

Preceding gene: 158338164

Following gene: 158338166

Centisome position: 78.46

GC content: 51.59

Gene sequence:

>1254_bases
GTGGGATTGATGGACGAGACGCTCAAACTCCTAGTGATTGATGATGATGAAGTCGATCGGATGGCCGTTCGGCGGGCCTT
AGGCAAAACCAATTTAGAGGTGGATCTGTCTGAAGCAGAGGATGGAGAAGCGGCCCTGTCTCTTCTTAAAAACCAGCAAT
TTGACTGTATTTTCGTCGATTTTTTACTGCCTGATCGGGATGGGTTGTCCTTAGCCAAGCAGCTGCAGCGATTGCAGGTA
GACACCCCCTTTGTAGTACTGACGGGACAAGGGGATGAGCAAATTGCAGTAGATGTTATGAAAGCTGGAGCCGCCGATTA
TCTGGCGAAACATCGGATTACACCGACAACTTTGGCTCGAACGATTCGCAGTGCCATTCGCCTCCATCGAGCTGAACAAG
CCGCCCGCGCTGCCAAGCAGAAATTGCAAATCACCAATGATCAGTTACGGCAACAGAATGAAGAACTGGCCCACCATCGG
GCTCAGATTGAAGCCCAGAACCAAGAGCTGATTAAAGCCTATCGCCTCAAGTCTGAGTTTATTGCCACCATTTCCCACGA
GCTGCGAACGCCCATGAATGCAATTATGGGATTCTCTCAATTACTCTTGCGCCAGTATCCCGATCCCTTGAGTGAGCAGC
AGTTGGACATGGTGCAGCGGATTTTTGACAACAGCCGCAATTTGTTGACTATGGTCAATGAAGTGTTGGACTTCTCTAAG
ATTGAGGCCAATCGCCTCGAACTGCACCCCGAACCCTTTCAACTCTCGGTTCCCATCCTGGCAACGATACAAGAACTCCA
GGCCCTGGCCGTAGAAAAGGCTCTGACCTTAAAGGCGGAGATCCCGGAGACGCTGCAATCCATCCAGTTGACCAACGACC
CTGAATGCCTCCGCCGGGTCCTGGTCAATCTGATTTCCAATGCCATCAAATTTACCGAAGCGGGTGAAGTGACGCTCCAG
CTCCACCAGCCCCAGCCCGACCAGATCAAAATTTCGGTGGTGGATACGGGCATTGGCATGACGCCTGAGCAGCTAAGCAC
CATCTTTGATCCCTTCTGTCAGGCCGATCAGTCTCTAACCCGCCATCATTCCGGGACTGGGTTGGGCCTTGCGATCACGG
AATCCTTGGTTAAAGCCATGCAGGGGCAGATTTCAGTGAGCAGTCAACCGGGAGTTGGCTCAACTTTTACCGTTGAATTG
CCCCTACAAGTGCAGACCTTGCCGAATGCCAAAGCTAAATCCTCTGTAATCTAG

Upstream 100 bases:

>100_bases
ACAATGTGGCCGGTTATATCCTGAAACCCGTAACGTTTTCCAACTTTGCAGAGGTGATGACCACCCTCAATAAATACTGG
ACCCTTTGCGAAATGCTGTA

Downstream 100 bases:

>100_bases
TGCCCCCTTTGAGAAGTTTATGTCGCCTGCTTTATTGTCTAACCGCTATATTCTGGCCGTTGACGATCTGCCAGATAATT
TGATGCTGCTCAAAATGATG

Product: two-component hybrid histidine kinase sensor and regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MGLMDETLKLLVIDDDEVDRMAVRRALGKTNLEVDLSEAEDGEAALSLLKNQQFDCIFVDFLLPDRDGLSLAKQLQRLQV
DTPFVVLTGQGDEQIAVDVMKAGAADYLAKHRITPTTLARTIRSAIRLHRAEQAARAAKQKLQITNDQLRQQNEELAHHR
AQIEAQNQELIKAYRLKSEFIATISHELRTPMNAIMGFSQLLLRQYPDPLSEQQLDMVQRIFDNSRNLLTMVNEVLDFSK
IEANRLELHPEPFQLSVPILATIQELQALAVEKALTLKAEIPETLQSIQLTNDPECLRRVLVNLISNAIKFTEAGEVTLQ
LHQPQPDQIKISVVDTGIGMTPEQLSTIFDPFCQADQSLTRHHSGTGLGLAITESLVKAMQGQISVSSQPGVGSTFTVEL
PLQVQTLPNAKAKSSVI

Sequences:

>Translated_417_residues
MGLMDETLKLLVIDDDEVDRMAVRRALGKTNLEVDLSEAEDGEAALSLLKNQQFDCIFVDFLLPDRDGLSLAKQLQRLQV
DTPFVVLTGQGDEQIAVDVMKAGAADYLAKHRITPTTLARTIRSAIRLHRAEQAARAAKQKLQITNDQLRQQNEELAHHR
AQIEAQNQELIKAYRLKSEFIATISHELRTPMNAIMGFSQLLLRQYPDPLSEQQLDMVQRIFDNSRNLLTMVNEVLDFSK
IEANRLELHPEPFQLSVPILATIQELQALAVEKALTLKAEIPETLQSIQLTNDPECLRRVLVNLISNAIKFTEAGEVTLQ
LHQPQPDQIKISVVDTGIGMTPEQLSTIFDPFCQADQSLTRHHSGTGLGLAITESLVKAMQGQISVSSQPGVGSTFTVEL
PLQVQTLPNAKAKSSVI
>Mature_416_residues
GLMDETLKLLVIDDDEVDRMAVRRALGKTNLEVDLSEAEDGEAALSLLKNQQFDCIFVDFLLPDRDGLSLAKQLQRLQVD
TPFVVLTGQGDEQIAVDVMKAGAADYLAKHRITPTTLARTIRSAIRLHRAEQAARAAKQKLQITNDQLRQQNEELAHHRA
QIEAQNQELIKAYRLKSEFIATISHELRTPMNAIMGFSQLLLRQYPDPLSEQQLDMVQRIFDNSRNLLTMVNEVLDFSKI
EANRLELHPEPFQLSVPILATIQELQALAVEKALTLKAEIPETLQSIQLTNDPECLRRVLVNLISNAIKFTEAGEVTLQL
HQPQPDQIKISVVDTGIGMTPEQLSTIFDPFCQADQSLTRHHSGTGLGLAITESLVKAMQGQISVSSQPGVGSTFTVELP
LQVQTLPNAKAKSSVI

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=295, Percent_Identity=37.9661016949153, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI145693157, Length=284, Percent_Identity=37.3239436619718, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87081816, Length=285, Percent_Identity=35.4385964912281, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788713, Length=260, Percent_Identity=35, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI48994928, Length=231, Percent_Identity=38.0952380952381, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788549, Length=243, Percent_Identity=32.9218106995885, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=29.7413793103448, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=29.6943231441048, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786783, Length=228, Percent_Identity=29.8245614035088, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=31.6964285714286, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=28.1938325991189, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788393, Length=240, Percent_Identity=27.9166666666667, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787894, Length=229, Percent_Identity=28.3842794759825, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790346, Length=235, Percent_Identity=28.936170212766, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790300, Length=277, Percent_Identity=25.2707581227437, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788279, Length=234, Percent_Identity=26.0683760683761, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790861, Length=251, Percent_Identity=26.6932270916335, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=228, Percent_Identity=24.5614035087719, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322044, Length=91, Percent_Identity=42.8571428571429, Blast_Score=83, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46359; Mature: 46228

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLMDETLKLLVIDDDEVDRMAVRRALGKTNLEVDLSEAEDGEAALSLLKNQQFDCIFVD
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEE
FLLPDRDGLSLAKQLQRLQVDTPFVVLTGQGDEQIAVDVMKAGAADYLAKHRITPTTLAR
EECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHCCCCHHHHHH
TIRSAIRLHRAEQAARAAKQKLQITNDQLRQQNEELAHHRAQIEAQNQELIKAYRLKSEF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IATISHELRTPMNAIMGFSQLLLRQYPDPLSEQQLDMVQRIFDNSRNLLTMVNEVLDFSK
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IEANRLELHPEPFQLSVPILATIQELQALAVEKALTLKAEIPETLQSIQLTNDPECLRRV
CCCCCEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LVNLISNAIKFTEAGEVTLQLHQPQPDQIKISVVDTGIGMTPEQLSTIFDPFCQADQSLT
HHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
RHHSGTGLGLAITESLVKAMQGQISVSSQPGVGSTFTVELPLQVQTLPNAKAKSSVI
HHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
GLMDETLKLLVIDDDEVDRMAVRRALGKTNLEVDLSEAEDGEAALSLLKNQQFDCIFVD
CCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEE
FLLPDRDGLSLAKQLQRLQVDTPFVVLTGQGDEQIAVDVMKAGAADYLAKHRITPTTLAR
EECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHCCCCHHHHHH
TIRSAIRLHRAEQAARAAKQKLQITNDQLRQQNEELAHHRAQIEAQNQELIKAYRLKSEF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IATISHELRTPMNAIMGFSQLLLRQYPDPLSEQQLDMVQRIFDNSRNLLTMVNEVLDFSK
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IEANRLELHPEPFQLSVPILATIQELQALAVEKALTLKAEIPETLQSIQLTNDPECLRRV
CCCCCEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LVNLISNAIKFTEAGEVTLQLHQPQPDQIKISVVDTGIGMTPEQLSTIFDPFCQADQSLT
HHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
RHHSGTGLGLAITESLVKAMQGQISVSSQPGVGSTFTVELPLQVQTLPNAKAKSSVI
HHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]