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The map label for this gene is yfgF [C]

Identifier: 158338090

GI number: 158338090

Start: 5024262

End: 5026076

Strand: Reverse

Name: yfgF [C]

Synonym: AM1_4980

Alternate gene names: 158338090

Gene position: 5026076-5024262 (Counterclockwise)

Preceding gene: 158338091

Following gene: 158338085

Centisome position: 77.28

GC content: 40.22

Gene sequence:

>1815_bases
ATGCGATCAAGAAAAATACATGTATTAATCATTGAAGATGACGAACAAGATGCCAATATCGTTCGCCGTTATTTAGATAA
CATGCCTCAACTACCGTTAATACTCCAACATAAGCACACTGTACGGGAAGGCATCCATGTTTTACAAAACAACGATTTTA
TTGATCTAATCCTACTAGATCTCACTTTATCTGATGCCAAAGGTTTAGATACATTCCATCAAATTCATCAAGCTTTTCCA
CAAAAGCCTCTGATCATTCTGAGTGGCAATTCAAACCCAGAAATCATTAAAAAAACCCTACATGAGGGAGCAGTAGACTT
CTTAATTAAAGGAACTTTCGATAGCCCCTCTTTATGTTACTCCATTCAACATGCTTTAGAACTCCAGAAACAAAAACTAG
AGCTAGAGCTTCAGAATAGTACTCTTCAATCATTATCGAAACAACTAGAAATAGCTCAGGCTGAAATCAAGAAACTTAAA
ACAATTGACTCTCTAACTAAGTTTTATAATCGAACTCAGTTTGATGACGTTTTCCTATCTGAATGGAAAAGACTACAGCG
AGAAGAACAACCCTTAGCTCTCATTCTTTGCGAGCTAGACCCGATAGCGACTGAGATGGATCCAGTTTCAACAACCTGGG
AAGATCATACGTTGCATAAAATCGCTGAGATCATGTTCCACACCATTAAGCGTCCAGCAGATTGTGTCGCTCGATATAGT
GAAAATCAATTTGTAATCTTGTTACCCAATACTGATATTTTGGGAGCAACCTTCATTGCAGAAGCCATCCGCATTCAAGT
TCAAAAACTTGGCATAGCTTATCTTTCTAGCCCATACCATCAACCCATCACCCTCAGTTTTGGAGTCACAGGCCATATCC
CAAGGCATCCAGAAGCGCCCTCTTTATTGATTGAAGCTGCACACCAAGCATTGATAGGCGCTAAAGCGCGTGGAAGGCAT
CAGGTCAATTGGCAAACCATTCGGGACATCAAATCGGAGTTATACACTCGCCAAACATTACATTGGGTGAGCAAGTTACA
TCAGGCATTACAAAACGATCTGTTTCAGCTCTATGTTCAACCCATTCACCCATTAAATAGAACAAGTAGCATAGAGCGTC
TCGACATCTTACTCAGACTCTGTGATCAATCAGGCAGAGCTTATGCACCCGAAATGTTTCTGCCCAGGATTGAACAGTAT
GATTTTATGGCTCAGATTGACCAGTGGGTGATTGAGAACTTGTTATTAGAACATACACAGATTTATTCAAATAGAAAGTT
TGACGCTGTTTATTTTATTAAACTCTCAGCAGCAACCTGTAAGGCAGGAAATCTATCTGAGCTGGTACAACAACAGCTCA
ATCTATACGATTTCCCAGCGAATCACCTTTGCTTTGCAATCTCAGAATCAGTCGCCTTGAAGGATTTATCCTCAGCAGTG
CAGCTGGCACAATCCCTTAAAGCATTAGGCTGTCAGATTGCCATAGATGATTTTGGTGTAGATTTCAAGACGCTTAGACG
GCTTAGACCGATGAACGCAGATTACATAAAAATCAACGGAGAATTGGTTGAAAGCATTACTACAGATCCCATATTGCATG
GAATTGTAGGGTCAATAAAGCGTGTGGCTGATGTTATGAATTCAAAAATTATTGCCAAGCAAGTTAAATCAGAGGTGACC
CTTAACTCGCTAGATAACTTAGGAATTAACTATGCCCAAGGGAATCATTTTGAACAGTCCCTTCCCCTTGAACAGTACAT
CAGTAGCAACCCTCACCGATGGCTTACTAATTTAGATGCATGTCTTTCACCCTAG

Upstream 100 bases:

>100_bases
GAAGTACCTTACTTCCAAATGCATCAAAAACCGCATAAAACAGAGAATAGGAAGCAGGTCTTTTTTTATTGAATGTAGAT
TTGCATTTAAGGGGGAGAAA

Downstream 100 bases:

>100_bases
AAGCATGAATACAGCAGCCAGTAAAGGTCAAAGTCAAAAATTACGTTTGAATCAGTTCCATAGTGTGAGTGCGATTGCGA
CCTCGCTTTTTAGCCATATA

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MRSRKIHVLIIEDDEQDANIVRRYLDNMPQLPLILQHKHTVREGIHVLQNNDFIDLILLDLTLSDAKGLDTFHQIHQAFP
QKPLIILSGNSNPEIIKKTLHEGAVDFLIKGTFDSPSLCYSIQHALELQKQKLELELQNSTLQSLSKQLEIAQAEIKKLK
TIDSLTKFYNRTQFDDVFLSEWKRLQREEQPLALILCELDPIATEMDPVSTTWEDHTLHKIAEIMFHTIKRPADCVARYS
ENQFVILLPNTDILGATFIAEAIRIQVQKLGIAYLSSPYHQPITLSFGVTGHIPRHPEAPSLLIEAAHQALIGAKARGRH
QVNWQTIRDIKSELYTRQTLHWVSKLHQALQNDLFQLYVQPIHPLNRTSSIERLDILLRLCDQSGRAYAPEMFLPRIEQY
DFMAQIDQWVIENLLLEHTQIYSNRKFDAVYFIKLSAATCKAGNLSELVQQQLNLYDFPANHLCFAISESVALKDLSSAV
QLAQSLKALGCQIAIDDFGVDFKTLRRLRPMNADYIKINGELVESITTDPILHGIVGSIKRVADVMNSKIIAKQVKSEVT
LNSLDNLGINYAQGNHFEQSLPLEQYISSNPHRWLTNLDACLSP

Sequences:

>Translated_604_residues
MRSRKIHVLIIEDDEQDANIVRRYLDNMPQLPLILQHKHTVREGIHVLQNNDFIDLILLDLTLSDAKGLDTFHQIHQAFP
QKPLIILSGNSNPEIIKKTLHEGAVDFLIKGTFDSPSLCYSIQHALELQKQKLELELQNSTLQSLSKQLEIAQAEIKKLK
TIDSLTKFYNRTQFDDVFLSEWKRLQREEQPLALILCELDPIATEMDPVSTTWEDHTLHKIAEIMFHTIKRPADCVARYS
ENQFVILLPNTDILGATFIAEAIRIQVQKLGIAYLSSPYHQPITLSFGVTGHIPRHPEAPSLLIEAAHQALIGAKARGRH
QVNWQTIRDIKSELYTRQTLHWVSKLHQALQNDLFQLYVQPIHPLNRTSSIERLDILLRLCDQSGRAYAPEMFLPRIEQY
DFMAQIDQWVIENLLLEHTQIYSNRKFDAVYFIKLSAATCKAGNLSELVQQQLNLYDFPANHLCFAISESVALKDLSSAV
QLAQSLKALGCQIAIDDFGVDFKTLRRLRPMNADYIKINGELVESITTDPILHGIVGSIKRVADVMNSKIIAKQVKSEVT
LNSLDNLGINYAQGNHFEQSLPLEQYISSNPHRWLTNLDACLSP
>Mature_604_residues
MRSRKIHVLIIEDDEQDANIVRRYLDNMPQLPLILQHKHTVREGIHVLQNNDFIDLILLDLTLSDAKGLDTFHQIHQAFP
QKPLIILSGNSNPEIIKKTLHEGAVDFLIKGTFDSPSLCYSIQHALELQKQKLELELQNSTLQSLSKQLEIAQAEIKKLK
TIDSLTKFYNRTQFDDVFLSEWKRLQREEQPLALILCELDPIATEMDPVSTTWEDHTLHKIAEIMFHTIKRPADCVARYS
ENQFVILLPNTDILGATFIAEAIRIQVQKLGIAYLSSPYHQPITLSFGVTGHIPRHPEAPSLLIEAAHQALIGAKARGRH
QVNWQTIRDIKSELYTRQTLHWVSKLHQALQNDLFQLYVQPIHPLNRTSSIERLDILLRLCDQSGRAYAPEMFLPRIEQY
DFMAQIDQWVIENLLLEHTQIYSNRKFDAVYFIKLSAATCKAGNLSELVQQQLNLYDFPANHLCFAISESVALKDLSSAV
QLAQSLKALGCQIAIDDFGVDFKTLRRLRPMNADYIKINGELVESITTDPILHGIVGSIKRVADVMNSKIIAKQVKSEVT
LNSLDNLGINYAQGNHFEQSLPLEQYISSNPHRWLTNLDACLSP

Specific function: Unknown

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MHYT domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=243, Percent_Identity=34.156378600823, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI87081921, Length=444, Percent_Identity=22.7477477477477, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790496, Length=255, Percent_Identity=29.4117647058824, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI226510982, Length=286, Percent_Identity=25.1748251748252, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787541, Length=436, Percent_Identity=22.4770642201835, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI87082096, Length=259, Percent_Identity=29.3436293436293, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788502, Length=253, Percent_Identity=23.3201581027668, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081980, Length=257, Percent_Identity=22.9571984435798, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1790566, Length=106, Percent_Identity=36.7924528301887, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787055, Length=256, Percent_Identity=25.390625, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI145693134, Length=197, Percent_Identity=26.9035532994924, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 68868; Mature: 68868

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRKIHVLIIEDDEQDANIVRRYLDNMPQLPLILQHKHTVREGIHVLQNNDFIDLILLD
CCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEEEE
LTLSDAKGLDTFHQIHQAFPQKPLIILSGNSNPEIIKKTLHEGAVDFLIKGTFDSPSLCY
EEECCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCHHHH
SIQHALELQKQKLELELQNSTLQSLSKQLEIAQAEIKKLKTIDSLTKFYNRTQFDDVFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EWKRLQREEQPLALILCELDPIATEMDPVSTTWEDHTLHKIAEIMFHTIKRPADCVARYS
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCC
ENQFVILLPNTDILGATFIAEAIRIQVQKLGIAYLSSPYHQPITLSFGVTGHIPRHPEAP
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCH
SLLIEAAHQALIGAKARGRHQVNWQTIRDIKSELYTRQTLHWVSKLHQALQNDLFQLYVQ
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIHPLNRTSSIERLDILLRLCDQSGRAYAPEMFLPRIEQYDFMAQIDQWVIENLLLEHTQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
IYSNRKFDAVYFIKLSAATCKAGNLSELVQQQLNLYDFPANHLCFAISESVALKDLSSAV
HHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHHHHHHH
QLAQSLKALGCQIAIDDFGVDFKTLRRLRPMNADYIKINGELVESITTDPILHGIVGSIK
HHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCCCEEEECHHHHHHHCCHHHHHHHHHHHH
RVADVMNSKIIAKQVKSEVTLNSLDNLGINYAQGNHFEQSLPLEQYISSNPHRWLTNLDA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHCCCCCHHHHHHHH
CLSP
HCCC
>Mature Secondary Structure
MRSRKIHVLIIEDDEQDANIVRRYLDNMPQLPLILQHKHTVREGIHVLQNNDFIDLILLD
CCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEEEE
LTLSDAKGLDTFHQIHQAFPQKPLIILSGNSNPEIIKKTLHEGAVDFLIKGTFDSPSLCY
EEECCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCHHHH
SIQHALELQKQKLELELQNSTLQSLSKQLEIAQAEIKKLKTIDSLTKFYNRTQFDDVFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EWKRLQREEQPLALILCELDPIATEMDPVSTTWEDHTLHKIAEIMFHTIKRPADCVARYS
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCC
ENQFVILLPNTDILGATFIAEAIRIQVQKLGIAYLSSPYHQPITLSFGVTGHIPRHPEAP
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCH
SLLIEAAHQALIGAKARGRHQVNWQTIRDIKSELYTRQTLHWVSKLHQALQNDLFQLYVQ
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIHPLNRTSSIERLDILLRLCDQSGRAYAPEMFLPRIEQYDFMAQIDQWVIENLLLEHTQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
IYSNRKFDAVYFIKLSAATCKAGNLSELVQQQLNLYDFPANHLCFAISESVALKDLSSAV
HHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHEEEHHHHHHHHHHHHHHH
QLAQSLKALGCQIAIDDFGVDFKTLRRLRPMNADYIKINGELVESITTDPILHGIVGSIK
HHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCCCEEEECHHHHHHHCCHHHHHHHHHHHH
RVADVMNSKIIAKQVKSEVTLNSLDNLGINYAQGNHFEQSLPLEQYISSNPHRWLTNLDA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHCCCCCHHHHHHHH
CLSP
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]