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The map label for this gene is liaR [H]

Identifier: 158338061

GI number: 158338061

Start: 4986028

End: 4987047

Strand: Direct

Name: liaR [H]

Synonym: AM1_4948

Alternate gene names: 158338061

Gene position: 4986028-4987047 (Clockwise)

Preceding gene: 158338060

Following gene: 158338062

Centisome position: 76.66

GC content: 44.31

Gene sequence:

>1020_bases
ATGATCCGCATTCTTCTAGTTGATGATCAGAAAGTGATTCGCCAAGGACTCAAAGCCTTGTTGGAATCAGAATCTGACTT
GCAGATTGTAGGACTGGCCGAAAACGGCAAAATGGGTGTGTCAATGGCAGAGCAACTTCAGCCTGATGTTGTTTTGGTTG
ATATGTATATGCCCATCATGGATGGCGCTGCAGCCACAGAGATTATTTGCCAGCGCTGTCCCGATACCAAAGTCTTACTA
TTGAGCAGCGCAGATGATGACAAATGCATTGCGGATGCTTTACGAGCAGGGGCAATGGGATATCTTCTTAAGAATGCATC
TTCCGAAGAAGTTGCCAATGCAATTCGCTCTGTTTATAAGGGGTATACCCAACTGAGTCCGGGCCTCTTAAAGAAAGTAA
TGAATCGCAACCCTCCCACCACAGAGGCGGAGACTTCTTCTGCGGACCTTACCGCCCTGGAAAAGCGGTTGCTGCTCATC
CTTAAGGACTCGGACACTTTAGACAGTTCCAGCATTGACGCTTTTACAGGCTTGATTTCTTCTCCATCTGAAGCGAAGAA
GCTGTTGCCCCACTTGAAGCAAAAACTGGAGCGACAACCAGATCATGTTCTGGCTTATTATATTCTGGGCATTTTATTAT
TCGAGTATCAACATCAGGCCAAAGTCGCTTTGAACTGTTGGCGCAAAGGATTTAAACAAGCTCTGTCACAAGGGATATCT
CTAGAAGGCTTATTTCTCTTTTGTCAGTCCATTTCTTTAATCAGTCCCGATGAAGCTTATATCGGCATCAAGCAAGTTCT
AGACACCCATGGAAAGCTTGTTCCTGCATCCACTCTAGGAGCAATGGCAGAACGAGTGTTTGGCCCTGAATCTGAGTGTA
CTCAAGTTCTAACCATTGCATGGCAAATTAGACAGATGAACACTTTACTTGATGAACATTACCCCTTAAAGTCAAAGCTT
GACGACTTAAATATTAGGTTTGCAATGCCTGGAAGAAAAAAACAAGTTTATAGCCTTTGA

Upstream 100 bases:

>100_bases
TAATTTTGATTAAGATGATGATCATTTAATCTGGTGGTCCATAATATGTGGACCAGGCCAGTGAAAGTAGCCCGATCTTT
TTATTAGATAATCCCCAACA

Downstream 100 bases:

>100_bases
GGAGACGGGTTTTGAGTGCAATTGATCAACTGGAGAAAGAATTAGAGGATCTGCGAAGTCGGTTTAGTCAAGCTTTTCAG
GTGTTGGATGGCTTTTCTCA

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MIRILLVDDQKVIRQGLKALLESESDLQIVGLAENGKMGVSMAEQLQPDVVLVDMYMPIMDGAAATEIICQRCPDTKVLL
LSSADDDKCIADALRAGAMGYLLKNASSEEVANAIRSVYKGYTQLSPGLLKKVMNRNPPTTEAETSSADLTALEKRLLLI
LKDSDTLDSSSIDAFTGLISSPSEAKKLLPHLKQKLERQPDHVLAYYILGILLFEYQHQAKVALNCWRKGFKQALSQGIS
LEGLFLFCQSISLISPDEAYIGIKQVLDTHGKLVPASTLGAMAERVFGPESECTQVLTIAWQIRQMNTLLDEHYPLKSKL
DDLNIRFAMPGRKKQVYSL

Sequences:

>Translated_339_residues
MIRILLVDDQKVIRQGLKALLESESDLQIVGLAENGKMGVSMAEQLQPDVVLVDMYMPIMDGAAATEIICQRCPDTKVLL
LSSADDDKCIADALRAGAMGYLLKNASSEEVANAIRSVYKGYTQLSPGLLKKVMNRNPPTTEAETSSADLTALEKRLLLI
LKDSDTLDSSSIDAFTGLISSPSEAKKLLPHLKQKLERQPDHVLAYYILGILLFEYQHQAKVALNCWRKGFKQALSQGIS
LEGLFLFCQSISLISPDEAYIGIKQVLDTHGKLVPASTLGAMAERVFGPESECTQVLTIAWQIRQMNTLLDEHYPLKSKL
DDLNIRFAMPGRKKQVYSL
>Mature_339_residues
MIRILLVDDQKVIRQGLKALLESESDLQIVGLAENGKMGVSMAEQLQPDVVLVDMYMPIMDGAAATEIICQRCPDTKVLL
LSSADDDKCIADALRAGAMGYLLKNASSEEVANAIRSVYKGYTQLSPGLLKKVMNRNPPTTEAETSSADLTALEKRLLLI
LKDSDTLDSSSIDAFTGLISSPSEAKKLLPHLKQKLERQPDHVLAYYILGILLFEYQHQAKVALNCWRKGFKQALSQGIS
LEGLFLFCQSISLISPDEAYIGIKQVLDTHGKLVPASTLGAMAERVFGPESECTQVLTIAWQIRQMNTLLDEHYPLKSKL
DDLNIRFAMPGRKKQVYSL

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=171, Percent_Identity=32.1637426900585, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787473, Length=158, Percent_Identity=32.9113924050633, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790102, Length=150, Percent_Identity=30.6666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786747, Length=159, Percent_Identity=25.7861635220126, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788521, Length=125, Percent_Identity=29.6, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=31.7307692307692, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 37443; Mature: 37443

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRILLVDDQKVIRQGLKALLESESDLQIVGLAENGKMGVSMAEQLQPDVVLVDMYMPIM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCEEEEEEHHHHH
DGAAATEIICQRCPDTKVLLLSSADDDKCIADALRAGAMGYLLKNASSEEVANAIRSVYK
CCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GYTQLSPGLLKKVMNRNPPTTEAETSSADLTALEKRLLLILKDSDTLDSSSIDAFTGLIS
HHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHC
SPSEAKKLLPHLKQKLERQPDHVLAYYILGILLFEYQHQAKVALNCWRKGFKQALSQGIS
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LEGLFLFCQSISLISPDEAYIGIKQVLDTHGKLVPASTLGAMAERVFGPESECTQVLTIA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
WQIRQMNTLLDEHYPLKSKLDDLNIRFAMPGRKKQVYSL
HHHHHHHHHHHCCCCCHHCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MIRILLVDDQKVIRQGLKALLESESDLQIVGLAENGKMGVSMAEQLQPDVVLVDMYMPIM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHCCCCEEEEEEHHHHH
DGAAATEIICQRCPDTKVLLLSSADDDKCIADALRAGAMGYLLKNASSEEVANAIRSVYK
CCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GYTQLSPGLLKKVMNRNPPTTEAETSSADLTALEKRLLLILKDSDTLDSSSIDAFTGLIS
HHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHC
SPSEAKKLLPHLKQKLERQPDHVLAYYILGILLFEYQHQAKVALNCWRKGFKQALSQGIS
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LEGLFLFCQSISLISPDEAYIGIKQVLDTHGKLVPASTLGAMAERVFGPESECTQVLTIA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
WQIRQMNTLLDEHYPLKSKLDDLNIRFAMPGRKKQVYSL
HHHHHHHHHHHCCCCCHHCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]