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The map label for this gene is folD

Identifier: 158337969

GI number: 158337969

Start: 4899840

End: 4900727

Strand: Reverse

Name: folD

Synonym: AM1_4855

Alternate gene names: 158337969

Gene position: 4900727-4899840 (Counterclockwise)

Preceding gene: 158337971

Following gene: 158337967

Centisome position: 75.35

GC content: 52.03

Gene sequence:

>888_bases
ATGCCTGTTGAGTCTTCCAAGCTGCTAGACGGCAAGGGACTAGCCCAAAAAATTCAAGGGGAGCTGACTGCCCAAATTCA
AACCTTACAAGCCGAAATCGGTCGTCCACCCGGATTAGCCGTTCTGATGGTGGGGGATAATCCGGCGAGTGCAGCCTATG
TGCGGAATAAAGAACGGGCCTGCGAACGGTTAGGGATTGCTTCTTTTGGTCACCATTTCCCTGCGGAGGTGACCTTTGAG
GAAGTGAAGCAAACCATCCACGACTTAAATGCGAATCCTCAAGTCGATGGCATTCTGGTGCAGTTGCCCTTGCCTGCCCA
TTTGGATTCCACAGCACTACTGTATGAGATTGATCCAGATAAAGATGTAGATGGTTTACATCCCCTAAACTTAGGGCGAT
TAGTGCGAGGTGAGGCAGGATTACGCAGCTGTACCCCTGCGGGGGTGATGCGTTTATTAGCAGCCTATGATGTGGAGCTG
TCCGGTCTGCATGCGGTTGTTATTGGCCGCAGTATTTTGGTAGGCAAACCCGTGTCTTTGATGCTGTTGGCAGCCAATGC
CACGGTGACGATGGCCCATTCTCGCACCCCTGATTTGGCCGCGGTTACCCGCACTGCTGATGTGTTGGTGGCGGCCGTCG
GCAAACCTGGACTGATCACGGCAGATATGGTTAAGCCCGGAGCTGTGGTGATTGATGTGGGGATAAGCCGCCAAGAAATT
GAAGGGAAAGCCCGCTTAGTAGGAGACGTGGAGTTTACCTCAGTCCAGTCTCAAAGTTCATTAATTACCCCAGTGCCAGG
GGGCGTCGGTCCCATAACCGTGTCTATGCTTTTGGAGAATACCGTGTGGAGTTTTAGGCAACGGCACAAAGGATCATACA
ATAGTTGA

Upstream 100 bases:

>100_bases
AAACTGTAACGTTGCCCCTTAGGTATAAGACTATCCCGAATTCTAAGGTAAGCCGATACAATCGAGAAGCACTTTTAACT
GCATCAGAAGGACGTTACCG

Downstream 100 bases:

>100_bases
ACTAGCCTCATGGTTGAGATCGCACTGAATCACTGCTTTCAACAGCTGGAGGGCTTTTTTTGCCCGACTTAGGGTGACCA
TTTCATCGTCAAAGATCACG

Product: bifunctional protein FolD

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MPVESSKLLDGKGLAQKIQGELTAQIQTLQAEIGRPPGLAVLMVGDNPASAAYVRNKERACERLGIASFGHHFPAEVTFE
EVKQTIHDLNANPQVDGILVQLPLPAHLDSTALLYEIDPDKDVDGLHPLNLGRLVRGEAGLRSCTPAGVMRLLAAYDVEL
SGLHAVVIGRSILVGKPVSLMLLAANATVTMAHSRTPDLAAVTRTADVLVAAVGKPGLITADMVKPGAVVIDVGISRQEI
EGKARLVGDVEFTSVQSQSSLITPVPGGVGPITVSMLLENTVWSFRQRHKGSYNS

Sequences:

>Translated_295_residues
MPVESSKLLDGKGLAQKIQGELTAQIQTLQAEIGRPPGLAVLMVGDNPASAAYVRNKERACERLGIASFGHHFPAEVTFE
EVKQTIHDLNANPQVDGILVQLPLPAHLDSTALLYEIDPDKDVDGLHPLNLGRLVRGEAGLRSCTPAGVMRLLAAYDVEL
SGLHAVVIGRSILVGKPVSLMLLAANATVTMAHSRTPDLAAVTRTADVLVAAVGKPGLITADMVKPGAVVIDVGISRQEI
EGKARLVGDVEFTSVQSQSSLITPVPGGVGPITVSMLLENTVWSFRQRHKGSYNS
>Mature_294_residues
PVESSKLLDGKGLAQKIQGELTAQIQTLQAEIGRPPGLAVLMVGDNPASAAYVRNKERACERLGIASFGHHFPAEVTFEE
VKQTIHDLNANPQVDGILVQLPLPAHLDSTALLYEIDPDKDVDGLHPLNLGRLVRGEAGLRSCTPAGVMRLLAAYDVELS
GLHAVVIGRSILVGKPVSLMLLAANATVTMAHSRTPDLAAVTRTADVLVAAVGKPGLITADMVKPGAVVIDVGISRQEIE
GKARLVGDVEFTSVQSQSSLITPVPGGVGPITVSMLLENTVWSFRQRHKGSYNS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=42.8082191780822, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI94721354, Length=292, Percent_Identity=42.1232876712329, Blast_Score=217, Evalue=9e-57,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=39.6551724137931, Blast_Score=204, Evalue=6e-53,
Organism=Escherichia coli, GI1786741, Length=285, Percent_Identity=47.719298245614, Blast_Score=266, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17568735, Length=264, Percent_Identity=37.8787878787879, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=42.8571428571429, Blast_Score=225, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=40.0662251655629, Blast_Score=217, Evalue=1e-57,
Organism=Drosophila melanogaster, GI17136818, Length=302, Percent_Identity=45.364238410596, Blast_Score=247, Evalue=7e-66,
Organism=Drosophila melanogaster, GI17136816, Length=302, Percent_Identity=45.364238410596, Blast_Score=247, Evalue=8e-66,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=41.2587412587413, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=41.2587412587413, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=41.2587412587413, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=41.2587412587413, Blast_Score=211, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_ACAM1 (B0C3J3)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519145.1
- ProteinModelPortal:   B0C3J3
- SMR:   B0C3J3
- GeneID:   5683656
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4855
- HOGENOM:   HBG328751
- OMA:   GIDNTKV
- BioCyc:   AMAR329726:AM1_4855-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31204; Mature: 31073

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVESSKLLDGKGLAQKIQGELTAQIQTLQAEIGRPPGLAVLMVGDNPASAAYVRNKERA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCHHHH
CERLGIASFGHHFPAEVTFEEVKQTIHDLNANPQVDGILVQLPLPAHLDSTALLYEIDPD
HHHCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEECCC
KDVDGLHPLNLGRLVRGEAGLRSCTPAGVMRLLAAYDVELSGLHAVVIGRSILVGKPVSL
CCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCEE
MLLAANATVTMAHSRTPDLAAVTRTADVLVAAVGKPGLITADMVKPGAVVIDVGISRQEI
EEEECCCEEEEEECCCCCHHHHHHHHHEEEEECCCCCEEECCCCCCCEEEEEECCCHHHC
EGKARLVGDVEFTSVQSQSSLITPVPGGVGPITVSMLLENTVWSFRQRHKGSYNS
CCCEEEEECEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PVESSKLLDGKGLAQKIQGELTAQIQTLQAEIGRPPGLAVLMVGDNPASAAYVRNKERA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCHHHH
CERLGIASFGHHFPAEVTFEEVKQTIHDLNANPQVDGILVQLPLPAHLDSTALLYEIDPD
HHHCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEECCC
KDVDGLHPLNLGRLVRGEAGLRSCTPAGVMRLLAAYDVELSGLHAVVIGRSILVGKPVSL
CCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCEE
MLLAANATVTMAHSRTPDLAAVTRTADVLVAAVGKPGLITADMVKPGAVVIDVGISRQEI
EEEECCCEEEEEECCCCCHHHHHHHHHEEEEECCCCCEEECCCCCCCEEEEEECCCHHHC
EGKARLVGDVEFTSVQSQSSLITPVPGGVGPITVSMLLENTVWSFRQRHKGSYNS
CCCEEEEECEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA