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The map label for this gene is ygcM [C]

Identifier: 158337937

GI number: 158337937

Start: 4860046

End: 4860921

Strand: Reverse

Name: ygcM [C]

Synonym: AM1_4824

Alternate gene names: 158337937

Gene position: 4860921-4860046 (Counterclockwise)

Preceding gene: 158337938

Following gene: 158337934

Centisome position: 74.74

GC content: 46.8

Gene sequence:

>876_bases
ATGAAATGTCTTATCCACCGACGCGCTGAGTTTTCTGCCAGCCATCGGTATTGGTTACCTGAGCTATCTAAATCAGAAAA
TCAGGAAAAATTTGGACAATGTACACGTTCCCCGGGCCATGGCCATAACTATGAATTGTTTGTCTCCATGTGGGGCGAAC
TCGATCAATATGGGATGGTTTTAAATCTCTCAAACGTCAAGCAAGTGATTAAGCGGGAGGTTACTGCCCCCCTTAATTTT
TCCTATCTGAATGAGGTATGGCCTGAGTTTAAGGAAACCCTACCCACTACGGAGCATCTGGCACGGGTGATCTGGCAGCG
GTTAGAACCCCACTTGCCAATCGTAAACATTCAACTTTTTGAACATCCCAAACTGTGGGCCGACTACAAAGGAGCTGGCA
TGGAAGCCTATTTAACTGTTGGAAGTCATTTTAGTGCTGCGCATCGATTAGCACTTCCCGAACTGAGTTTTGAAGAAAAC
TGCGAAATCTATGGCAAATGCGCTCGACCTCATGGTCATGGCCATAACTACCACCTAGAAGTGACCGTTAAAGGTGAGGT
GGATGCCCGGACAGGCATGATTGTGGATTTAGTCGCTCTCCAAAGCCTGGTCGACGATGTGGTCCTAGACCCCCTTGACC
ATACCTTCTTGAATAAAGACATTCCCTACTTTGAAAAGGTCGTTCCTACGGCTGAAAATATTGCATTCTATATCGCCAAA
CTCCTGCGAGAGCCGATCCTCAAAATTGGGGCAGAACTTCATCGCATCAAGCTGATTGAAAGTCCGAATAATTCCTGCGA
AGTGCTGTGCTCTGACTTATTTGATACAGCGCCAATGCTCTCTGGTCGAATGGGGGAACCTGCATTAGTGGGCTAA

Upstream 100 bases:

>100_bases
CTGGCTTAACCCAGAGAACCTGAGCCATAGGTTAAAATTTTAAGAAATGTTATCGCCGTGTCTCCAACGAAGAGCGGCTG
TTGCTACGGTTTGCCTTGTT

Downstream 100 bases:

>100_bases
TTTTCGGACATTAAGTCCCTGCTAGTCGAGATTGCCTTACCACTGTTCACCAGCCAAATACTCATCAGATTCCATTCGAC
CATAATCGAGCTTGATAATC

Product: 6-pyruvoyl-tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKCLIHRRAEFSASHRYWLPELSKSENQEKFGQCTRSPGHGHNYELFVSMWGELDQYGMVLNLSNVKQVIKREVTAPLNF
SYLNEVWPEFKETLPTTEHLARVIWQRLEPHLPIVNIQLFEHPKLWADYKGAGMEAYLTVGSHFSAAHRLALPELSFEEN
CEIYGKCARPHGHGHNYHLEVTVKGEVDARTGMIVDLVALQSLVDDVVLDPLDHTFLNKDIPYFEKVVPTAENIAFYIAK
LLREPILKIGAELHRIKLIESPNNSCEVLCSDLFDTAPMLSGRMGEPALVG

Sequences:

>Translated_291_residues
MKCLIHRRAEFSASHRYWLPELSKSENQEKFGQCTRSPGHGHNYELFVSMWGELDQYGMVLNLSNVKQVIKREVTAPLNF
SYLNEVWPEFKETLPTTEHLARVIWQRLEPHLPIVNIQLFEHPKLWADYKGAGMEAYLTVGSHFSAAHRLALPELSFEEN
CEIYGKCARPHGHGHNYHLEVTVKGEVDARTGMIVDLVALQSLVDDVVLDPLDHTFLNKDIPYFEKVVPTAENIAFYIAK
LLREPILKIGAELHRIKLIESPNNSCEVLCSDLFDTAPMLSGRMGEPALVG
>Mature_291_residues
MKCLIHRRAEFSASHRYWLPELSKSENQEKFGQCTRSPGHGHNYELFVSMWGELDQYGMVLNLSNVKQVIKREVTAPLNF
SYLNEVWPEFKETLPTTEHLARVIWQRLEPHLPIVNIQLFEHPKLWADYKGAGMEAYLTVGSHFSAAHRLALPELSFEEN
CEIYGKCARPHGHGHNYHLEVTVKGEVDARTGMIVDLVALQSLVDDVVLDPLDHTFLNKDIPYFEKVVPTAENIAFYIAK
LLREPILKIGAELHRIKLIESPNNSCEVLCSDLFDTAPMLSGRMGEPALVG

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=121, Percent_Identity=47.1074380165289, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI115532224, Length=121, Percent_Identity=42.1487603305785, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115532226, Length=121, Percent_Identity=42.1487603305785, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19550074, Length=129, Percent_Identity=44.9612403100775, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585378, Length=129, Percent_Identity=44.9612403100775, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 33130; Mature: 33130

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCLIHRRAEFSASHRYWLPELSKSENQEKFGQCTRSPGHGHNYELFVSMWGELDQYGMV
CCCHHCCCHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCHHHCCEE
LNLSNVKQVIKREVTAPLNFSYLNEVWPEFKETLPTTEHLARVIWQRLEPHLPIVNIQLF
EEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEE
EHPKLWADYKGAGMEAYLTVGSHFSAAHRLALPELSFEENCEIYGKCARPHGHGHNYHLE
CCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEE
VTVKGEVDARTGMIVDLVALQSLVDDVVLDPLDHTFLNKDIPYFEKVVPTAENIAFYIAK
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHCCCHHHHHHHHHH
LLREPILKIGAELHRIKLIESPNNSCEVLCSDLFDTAPMLSGRMGEPALVG
HHHHHHHHHCCHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKCLIHRRAEFSASHRYWLPELSKSENQEKFGQCTRSPGHGHNYELFVSMWGELDQYGMV
CCCHHCCCHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCHHHCCEE
LNLSNVKQVIKREVTAPLNFSYLNEVWPEFKETLPTTEHLARVIWQRLEPHLPIVNIQLF
EEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEE
EHPKLWADYKGAGMEAYLTVGSHFSAAHRLALPELSFEENCEIYGKCARPHGHGHNYHLE
CCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEE
VTVKGEVDARTGMIVDLVALQSLVDDVVLDPLDHTFLNKDIPYFEKVVPTAENIAFYIAK
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHCCCHHHHHHHHHH
LLREPILKIGAELHRIKLIESPNNSCEVLCSDLFDTAPMLSGRMGEPALVG
HHHHHHHHHCCHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA