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The map label for this gene is leuC

Identifier: 158337624

GI number: 158337624

Start: 4532440

End: 4533855

Strand: Reverse

Name: leuC

Synonym: AM1_4505

Alternate gene names: 158337624

Gene position: 4533855-4532440 (Counterclockwise)

Preceding gene: 158337626

Following gene: 158337622

Centisome position: 69.71

GC content: 50.71

Gene sequence:

>1416_bases
ATGAGCCAGGGCACGCTATTTGATAAAGTTTGGGACTTACATACCGTAGATGTACTCCCCTCTGGTCAGACCCAGTTATT
TATTGGCCTCCATCTTATCCATGAAGTGACGAGTCCTCAAGCATTTACGATGCTGAAAGATCGAGGGCTAAACGTTTTAT
ATCCACAACGAACGGTGGCGACAGTCGATCATATCGTCCCGACAGAAAATCAGGCGCGTCCCTTTGCCGATACTCTGGCA
GAAGAAATGATGCAGGCATTGGAGCAGAGCTGCCAAGACCATGGCATTACCTTTCATAACATTGGCTCAGGTAAGCAGGG
GATTGTCCATGTTATTGCACCTGAACAAGGTTTAACCCAACCAGGCATGACGATTGCCTGTGGTGATAGCCATACTTCAA
CCCATGGGGCGTTTGGTGCCATCTCATTTGGGATTGGTACAAGCCAAGTACGAGATGTGCTGGCTTCCCAGACCCTTGCC
CTTGCCAAACTCAAAGTGCGCAAAGTGGAAGTCAACGGGACTTTGCCTGCTGGTGTGTATGCCAAAGATGTGATCTTGCA
TATCATTCGCACCCTGGGAGTAAAAGGGGGTGTGGGGTATGCCTATGAATTTGCTGGAACTACCTTTGAGCAAATGACCA
TGGATGAGCGGATGACGGTGTGCAATATGTCCATCGAGGGTGGAGCACGCTGTGGCTACGTCAATCCTGACACCATCACT
TTTGAGTATCTCAAAGGACGGGAAGCAGCACCTAAGGGGGCAGATTGGGACCAAGCGGTGGCGTGGTGGCAGAGTATTCG
CAGTGATGCTGATGCTGTGTATGACGATGTGGTTGTATTTGATGCCCAGGATATTTCACCCACGGTGACTTGGGGCATTA
CGCCAGGTCAGGGCATTGGTATTGATGAGGCGATTCCTGGCCTCGACCATTTTACGGATGCCGATCAGCCCATTGCCTCT
GAAGCTTACAAGTATATGGACTTGCAGCCGGGGCAACCGATTCAAGGGACCAAGGTGGATGTCTGTTTTATTGGCAGTTG
TACCAATGGCAGAATCAGTGATCTACGCGAGGCTGCCCAGTTTGCTAAAGGCAACCAGGTTGCCGCTGGGGTAAAAGCTT
TTGTGGTACCCGGATCAGAGCAAGTTAAACAGCAGGCCGAGTCGGAAGGTTTACACCATATTTTTGAACAAGCTGGATTT
GAATGGCGAGAGCCGGGATGTTCCATGTGTTTGGCTATGAATCCCGATAAGCTCCAAGGGCGGCAGCTCAGTGCGTCTTC
TTCCAATCGGAATTTTAAAGGACGCCAGGGGTCTGCATCTGGGCGAACATTGCTGATGAGTCCGGCGATGGTAGTGGCAG
CTGCCATCAAAGGCGAAGTTGCAGATGTCCGCCAAATCCCTAAGGCAAGCCCTTAA

Upstream 100 bases:

>100_bases
TTCCCTACTGAACCGTCACTACCCCCCAGAATCTGCCCTAACCCCTTTCCATAATTGAGATAAAATTGGAGTCCGATGAT
ACCATTATTGACTTCGATAT

Downstream 100 bases:

>100_bases
TTCGGCCTAGTCAGCATCTTGCCTTTGCATTTGGAGCAGTGTGATTGATCGGGACATTCTGAGATAACCCCCCCATACCT
CAAAAGCTGATCAGTCTTCT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSQGTLFDKVWDLHTVDVLPSGQTQLFIGLHLIHEVTSPQAFTMLKDRGLNVLYPQRTVATVDHIVPTENQARPFADTLA
EEMMQALEQSCQDHGITFHNIGSGKQGIVHVIAPEQGLTQPGMTIACGDSHTSTHGAFGAISFGIGTSQVRDVLASQTLA
LAKLKVRKVEVNGTLPAGVYAKDVILHIIRTLGVKGGVGYAYEFAGTTFEQMTMDERMTVCNMSIEGGARCGYVNPDTIT
FEYLKGREAAPKGADWDQAVAWWQSIRSDADAVYDDVVVFDAQDISPTVTWGITPGQGIGIDEAIPGLDHFTDADQPIAS
EAYKYMDLQPGQPIQGTKVDVCFIGSCTNGRISDLREAAQFAKGNQVAAGVKAFVVPGSEQVKQQAESEGLHHIFEQAGF
EWREPGCSMCLAMNPDKLQGRQLSASSSNRNFKGRQGSASGRTLLMSPAMVVAAAIKGEVADVRQIPKASP

Sequences:

>Translated_471_residues
MSQGTLFDKVWDLHTVDVLPSGQTQLFIGLHLIHEVTSPQAFTMLKDRGLNVLYPQRTVATVDHIVPTENQARPFADTLA
EEMMQALEQSCQDHGITFHNIGSGKQGIVHVIAPEQGLTQPGMTIACGDSHTSTHGAFGAISFGIGTSQVRDVLASQTLA
LAKLKVRKVEVNGTLPAGVYAKDVILHIIRTLGVKGGVGYAYEFAGTTFEQMTMDERMTVCNMSIEGGARCGYVNPDTIT
FEYLKGREAAPKGADWDQAVAWWQSIRSDADAVYDDVVVFDAQDISPTVTWGITPGQGIGIDEAIPGLDHFTDADQPIAS
EAYKYMDLQPGQPIQGTKVDVCFIGSCTNGRISDLREAAQFAKGNQVAAGVKAFVVPGSEQVKQQAESEGLHHIFEQAGF
EWREPGCSMCLAMNPDKLQGRQLSASSSNRNFKGRQGSASGRTLLMSPAMVVAAAIKGEVADVRQIPKASP
>Mature_470_residues
SQGTLFDKVWDLHTVDVLPSGQTQLFIGLHLIHEVTSPQAFTMLKDRGLNVLYPQRTVATVDHIVPTENQARPFADTLAE
EMMQALEQSCQDHGITFHNIGSGKQGIVHVIAPEQGLTQPGMTIACGDSHTSTHGAFGAISFGIGTSQVRDVLASQTLAL
AKLKVRKVEVNGTLPAGVYAKDVILHIIRTLGVKGGVGYAYEFAGTTFEQMTMDERMTVCNMSIEGGARCGYVNPDTITF
EYLKGREAAPKGADWDQAVAWWQSIRSDADAVYDDVVVFDAQDISPTVTWGITPGQGIGIDEAIPGLDHFTDADQPIASE
AYKYMDLQPGQPIQGTKVDVCFIGSCTNGRISDLREAAQFAKGNQVAAGVKAFVVPGSEQVKQQAESEGLHHIFEQAGFE
WREPGCSMCLAMNPDKLQGRQLSASSSNRNFKGRQGSASGRTLLMSPAMVVAAAIKGEVADVRQIPKASP

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:
GO:0003861: 3-isopropylmalate dehydratase large subunit
GO:0008152: 3-isopropylmalate dehydratase large subunit
GO:0008652: 3-isopropylmalate dehydratase large subunit
GO:0009082: 3-isopropylmalate dehydratase large subunit
GO:0009098: 3-isopropylmalate dehydratase large subunit
GO:0016829: 3-isopropylmalate dehydratase large subunit
GO:0016836: 3-isopropylmalate dehydratase large subunit
GO:0046872: 3-isopropylmalate dehydratase large subunit
GO:0051536: 3-isopropylmalate dehydratase large subunit
GO:0051539: 3-isopropylmalate dehydratase large subunit

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=395, Percent_Identity=28.6075949367089, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI41352693, Length=388, Percent_Identity=23.9690721649485, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI8659555, Length=447, Percent_Identity=23.2662192393736, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=51.8358531317495, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1787531, Length=365, Percent_Identity=24.6575342465753, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI2367097, Length=130, Percent_Identity=28.4615384615385, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32564738, Length=409, Percent_Identity=29.0953545232274, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25149337, Length=409, Percent_Identity=29.0953545232274, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=317, Percent_Identity=29.3375394321767, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=412, Percent_Identity=24.2718446601942, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=49.3617021276596, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322261, Length=410, Percent_Identity=29.7560975609756, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=391, Percent_Identity=29.156010230179, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320440, Length=430, Percent_Identity=24.8837209302326, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI161076999, Length=443, Percent_Identity=27.5395033860045, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281365315, Length=443, Percent_Identity=27.5395033860045, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17864292, Length=443, Percent_Identity=27.5395033860045, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571643, Length=392, Percent_Identity=27.5510204081633, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24645686, Length=367, Percent_Identity=25.8855585831063, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17137564, Length=366, Percent_Identity=24.5901639344262, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_ACAM1 (B0CG35)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518799.1
- ProteinModelPortal:   B0CG35
- SMR:   B0CG35
- GeneID:   5683307
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4505
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   AMAR329726:AM1_4505-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50684; Mature: 50553

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQGTLFDKVWDLHTVDVLPSGQTQLFIGLHLIHEVTSPQAFTMLKDRGLNVLYPQRTVA
CCCCCCHHHHHCCEEEEECCCCCCEEEEHHHHHHHHCCCHHHHHHHHCCCEEECCCHHHH
TVDHIVPTENQARPFADTLAEEMMQALEQSCQDHGITFHNIGSGKQGIVHVIAPEQGLTQ
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGAISFGIGTSQVRDVLASQTLALAKLKVRKVEVNGTLPAGVY
CCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHH
AKDVILHIIRTLGVKGGVGYAYEFAGTTFEQMTMDERMTVCNMSIEGGARCGYVNPDTIT
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHEEEEEEEECCCCCCCCCCCCCEE
FEYLKGREAAPKGADWDQAVAWWQSIRSDADAVYDDVVVFDAQDISPTVTWGITPGQGIG
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCCCC
IDEAIPGLDHFTDADQPIASEAYKYMDLQPGQPIQGTKVDVCFIGSCTNGRISDLREAAQ
HHHHCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
FAKGNQVAAGVKAFVVPGSEQVKQQAESEGLHHIFEQAGFEWREPGCSMCLAMNPDKLQG
HHCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCC
RQLSASSSNRNFKGRQGSASGRTLLMSPAMVVAAAIKGEVADVRQIPKASP
CEECCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCHHHHHHCCCCCC
>Mature Secondary Structure 
SQGTLFDKVWDLHTVDVLPSGQTQLFIGLHLIHEVTSPQAFTMLKDRGLNVLYPQRTVA
CCCCCHHHHHCCEEEEECCCCCCEEEEHHHHHHHHCCCHHHHHHHHCCCEEECCCHHHH
TVDHIVPTENQARPFADTLAEEMMQALEQSCQDHGITFHNIGSGKQGIVHVIAPEQGLTQ
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGAISFGIGTSQVRDVLASQTLALAKLKVRKVEVNGTLPAGVY
CCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHH
AKDVILHIIRTLGVKGGVGYAYEFAGTTFEQMTMDERMTVCNMSIEGGARCGYVNPDTIT
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHEEEEEEEECCCCCCCCCCCCCEE
FEYLKGREAAPKGADWDQAVAWWQSIRSDADAVYDDVVVFDAQDISPTVTWGITPGQGIG
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCCCC
IDEAIPGLDHFTDADQPIASEAYKYMDLQPGQPIQGTKVDVCFIGSCTNGRISDLREAAQ
HHHHCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
FAKGNQVAAGVKAFVVPGSEQVKQQAESEGLHHIFEQAGFEWREPGCSMCLAMNPDKLQG
HHCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCC
RQLSASSSNRNFKGRQGSASGRTLLMSPAMVVAAAIKGEVADVRQIPKASP
CEECCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA