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The map label for this gene is ntcB [H]

Identifier: 158337508

GI number: 158337508

Start: 4417949

End: 4418878

Strand: Reverse

Name: ntcB [H]

Synonym: AM1_4387

Alternate gene names: 158337508

Gene position: 4418878-4417949 (Counterclockwise)

Preceding gene: 158337512

Following gene: 158337507

Centisome position: 67.94

GC content: 48.71

Gene sequence:

>930_bases
ATGCGGCTCGAGCAACTGCAAGCATTTTTATCGGTAGCCCAAACAGGGAGTTTCCAACAAGCGGCCAAGAAATGTGGGGT
GACCCAATCAACCGTAAGTCGGCAAATTCAAGGACTGGAATCTGAACTGGGGCTCCCCCTCTTACATCGCGGTGCTCAAA
GCAAACTCACCTTGGCAGGGGATCAGCTCCTCCCTCATGTGCGTAAAATTTTGACCGAATGGCAAGATGCCACCCAGGAA
ATCAATGATCTCCGGGCCGGTAAACAGCCTGAACTCTGCGTTGCGGCAATTCATTCCGTCTGTGCCCATTACTTACCGCC
AGTGCTCCAGAAATTTTGCCAGCTCTACCCAGAAGTCCAATTGAGGGTCACATCCTTGGGGAGCGACCGTTCTTTGAAAG
TCCTCCAAGATGGTTTGGTTGACTTAGCCATTGTGATGAACAATCCGTTACTAACAAGTCGCTCAGAAAATGTTGTTGAC
TGGCTGTACAACGAACCCATTCAAGTTCTGATGTCTTCAGAACATCCTTTGGCGTCCCACGGCGTTGTGCCTTGGCCTGA
GTTAGCCAAATTCCCACAAGTGATGTTCAAAGACGGGTATGGGATGCAGCGGTTGGTACAGGATCAGTTCAAAAGCCAAG
GTCTAGATCTGAAAGCGGTCCTTGAACTCAATACCCTAGATGCCTTTCGAGGCGTCATTCGCCAAGGAGAAATGATTTCT
CTATTACCCAGGGGGGCTTTAGTCGATTCTCTTAACGATCCCTCCCTGGCGATTCGAGACTTGGCTGCACCTGAAGCCAA
GGATATGGATCCACAATTAAACCTGACTCGCGAAGTTGTGATGGTGACCACTCAGGACCGCTTGTTAATTCCACCTATTG
CTAATTTTCGACGTTTAGTTCATGATTCATTCATCTCCAATATTCCATAA

Upstream 100 bases:

>100_bases
ACTTTCGCTAAAAAGAATACCGAAGTTGTATCGTTTCTTACCGAATAGCCGGGCCAAATTCGTTACTGTGCTCTCATGAC
TGTAATCTAGACTACACTTC

Downstream 100 bases:

>100_bases
AGCCCATTAAGAGGAGATGTGGGTAAATTGCTACACAAGTATCAAGGTGACCCTTGATTCTAGAGTTGGCAAAGTAAACA
GCCATGAGTCAGATTTTTAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRLEQLQAFLSVAQTGSFQQAAKKCGVTQSTVSRQIQGLESELGLPLLHRGAQSKLTLAGDQLLPHVRKILTEWQDATQE
INDLRAGKQPELCVAAIHSVCAHYLPPVLQKFCQLYPEVQLRVTSLGSDRSLKVLQDGLVDLAIVMNNPLLTSRSENVVD
WLYNEPIQVLMSSEHPLASHGVVPWPELAKFPQVMFKDGYGMQRLVQDQFKSQGLDLKAVLELNTLDAFRGVIRQGEMIS
LLPRGALVDSLNDPSLAIRDLAAPEAKDMDPQLNLTREVVMVTTQDRLLIPPIANFRRLVHDSFISNIP

Sequences:

>Translated_309_residues
MRLEQLQAFLSVAQTGSFQQAAKKCGVTQSTVSRQIQGLESELGLPLLHRGAQSKLTLAGDQLLPHVRKILTEWQDATQE
INDLRAGKQPELCVAAIHSVCAHYLPPVLQKFCQLYPEVQLRVTSLGSDRSLKVLQDGLVDLAIVMNNPLLTSRSENVVD
WLYNEPIQVLMSSEHPLASHGVVPWPELAKFPQVMFKDGYGMQRLVQDQFKSQGLDLKAVLELNTLDAFRGVIRQGEMIS
LLPRGALVDSLNDPSLAIRDLAAPEAKDMDPQLNLTREVVMVTTQDRLLIPPIANFRRLVHDSFISNIP
>Mature_309_residues
MRLEQLQAFLSVAQTGSFQQAAKKCGVTQSTVSRQIQGLESELGLPLLHRGAQSKLTLAGDQLLPHVRKILTEWQDATQE
INDLRAGKQPELCVAAIHSVCAHYLPPVLQKFCQLYPEVQLRVTSLGSDRSLKVLQDGLVDLAIVMNNPLLTSRSENVVD
WLYNEPIQVLMSSEHPLASHGVVPWPELAKFPQVMFKDGYGMQRLVQDQFKSQGLDLKAVLELNTLDAFRGVIRQGEMIS
LLPRGALVDSLNDPSLAIRDLAAPEAKDMDPQLNLTREVVMVTTQDRLLIPPIANFRRLVHDSFISNIP

Specific function: Seems to regulate utilization of fixed nitrogen by controlling the expression of a certain gene(s) involved in nitrogen metabolism [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=242, Percent_Identity=28.9256198347107, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1787879, Length=247, Percent_Identity=29.1497975708502, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI157672245, Length=256, Percent_Identity=26.171875, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790399, Length=245, Percent_Identity=26.9387755102041, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786401, Length=142, Percent_Identity=38.0281690140845, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081904, Length=296, Percent_Identity=25.3378378378378, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787530, Length=264, Percent_Identity=24.6212121212121, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=27.1255060728745, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788296, Length=249, Percent_Identity=25.7028112449799, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082132, Length=271, Percent_Identity=25.4612546125461, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788887, Length=185, Percent_Identity=30.8108108108108, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788481, Length=265, Percent_Identity=26.0377358490566, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787806, Length=250, Percent_Identity=24, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790208, Length=145, Percent_Identity=31.7241379310345, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789173, Length=186, Percent_Identity=32.258064516129, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789440, Length=146, Percent_Identity=27.3972602739726, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994962, Length=115, Percent_Identity=33.9130434782609, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI145693193, Length=303, Percent_Identity=26.0726072607261, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34381; Mature: 34381

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEQLQAFLSVAQTGSFQQAAKKCGVTQSTVSRQIQGLESELGLPLLHRGAQSKLTLAG
CCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEECH
DQLLPHVRKILTEWQDATQEINDLRAGKQPELCVAAIHSVCAHYLPPVLQKFCQLYPEVQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
LRVTSLGSDRSLKVLQDGLVDLAIVMNNPLLTSRSENVVDWLYNEPIQVLMSSEHPLASH
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHCCCCCCCCC
GVVPWPELAKFPQVMFKDGYGMQRLVQDQFKSQGLDLKAVLELNTLDAFRGVIRQGEMIS
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCEEE
LLPRGALVDSLNDPSLAIRDLAAPEAKDMDPQLNLTREVVMVTTQDRLLIPPIANFRRLV
CCCCCHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEECCCHHHHHHHH
HDSFISNIP
HHHHHHCCC
>Mature Secondary Structure
MRLEQLQAFLSVAQTGSFQQAAKKCGVTQSTVSRQIQGLESELGLPLLHRGAQSKLTLAG
CCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEECH
DQLLPHVRKILTEWQDATQEINDLRAGKQPELCVAAIHSVCAHYLPPVLQKFCQLYPEVQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
LRVTSLGSDRSLKVLQDGLVDLAIVMNNPLLTSRSENVVDWLYNEPIQVLMSSEHPLASH
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHCCCCCCCCC
GVVPWPELAKFPQVMFKDGYGMQRLVQDQFKSQGLDLKAVLELNTLDAFRGVIRQGEMIS
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCEEE
LLPRGALVDSLNDPSLAIRDLAAPEAKDMDPQLNLTREVVMVTTQDRLLIPPIANFRRLV
CCCCCHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEECCCHHHHHHHH
HDSFISNIP
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7814317 [H]