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The map label for this gene is lpdA [H]

Identifier: 158337482

GI number: 158337482

Start: 4389751

End: 4391190

Strand: Reverse

Name: lpdA [H]

Synonym: AM1_4361

Alternate gene names: 158337482

Gene position: 4391190-4389751 (Counterclockwise)

Preceding gene: 158337483

Following gene: 158337481

Centisome position: 67.52

GC content: 50.9

Gene sequence:

>1440_bases
GTGACCCAACAATTTGACTATGACCTGGTAATTATTGGCGCTGGTGTTGGCGGTCATGGTGCGGCTTTGCATGCAGTAGA
CTGTGGTCTGAAAACTGCCATTATTGAAGCTGCAGATATGGGAGGTACCTGTGTAAATCGGGGCTGCATCCCCTCTAAAG
CCTTGTTAGCAGCGTCCGGACGGGTGCGCGAACTCCGCGATCAACATCATCTGCAATCCCTAGGCATTCAACTGGGTCAA
GTGAACTTCGACCGGGGCCAAATTGCGGCCCATGCTGATAACCTTGTCGATACCATTCGCGGCAATCTCACCAATAGCCT
CACTCGCCTAAAAGTCGAGATTATTCACGGCTGGGGCAAAGTCATCGGTAACCAAAAAGTGGTCGTCAAGTCCGACGCAG
GTGAGCAGACCATTACTTCCCGTGACATTATCATCGCCAGTGGTTCAGTACCGTGGGTTCCCCCAGGAATCGAAATTGAT
GGCCGAACCGTCTTTACCAGTGACGATGCCATTCGTTTGTCTTGGCTCCCTGATTGGGTCGCGATTATCGGTAGCGGCTA
TATTGGCCTCGAATTTTCCGATGTCTACACCGCCTTGGGCAGTGAAGTGACGATTATCGAAGCTCTCGATACGTTAATGC
CCACCTTTGACCCGGATATTGCCAAAATTGCCAAACGCGTTTTAATCGATCCGCGAGATATTGACACCCATGCGGGTCGC
TTAGCTAAGAAGGTGACCCCCGGTTCTCCCGTTGTGATTGAGCTAGCGGATGTCAAGACCAAAGAAGTGGTTGAGGTCTT
AGAAGTAGATGCTTGCTTAGTGGCAACCGGGCGAATTCCAGCCACGGATAATTTGGGACTAGAGGCCATTAGTGTCGATA
CGGATCGACGAGGCTTTATCCCCGTCAACGATCGGATGCAGGTGTTGAGCCAAGGAGAAGTGGTCCCCCACGTCTATGCC
ATTGGTGATGCCACGGGCAAAATGATGCTGGCTCATGCCGCTTCTGCCCAAGGAATTGTAGTCGTGGAGAATATTTGCGA
ACGTCCTCGGGATGTTAATTACCGCAGTATTCCTGCCGCTGCTTTCACCCATCCTGAAATTAGTTTTGTCGGTTTAACGG
AACCCCAAGCCAAAGAGTTGGCCAAAACGGAAGGATTTAAAATAAACACAGTCCGGTCCTATTTCAAGGCCAATTCCAAA
GCCCTGGCAGAAAGTGAGGCAGATGGCCTCGCCAAATTAATCTACCGTGAAGACACAGGGGAGATTTTAGGGGGGCATAT
TATTGGTCTCCATGCCGCCGATCTGATTCATGAAGTCTCTAATGCCGTTGCTCAAGGGCAACCAGTCCAGAGCTTAAGCC
ATTTGGTACATACCCATCCGACCATTTCTGAAGTGATCGATGAGGCCTTCAAGCGAGCAGCCACAGGCTTCGCCCATTGA

Upstream 100 bases:

>100_bases
CCCGCTTGCTTAAAGCCCCGGCTCCAGTGAACAATAGGGAATGTAAGTTAGATGAATAATCATCTAACCCGCCGAATTGA
TTCCTGATCGGGAGAGCAAC

Downstream 100 bases:

>100_bases
TGGCAATCTGTTCTCGCTAAAAGCCCTGGAACAGTAAGGGGGGTGCTTAAATCGTTATAATTGTTGGACCCTAGCTGTTT
TGGTCTCCATGCAAATTCGC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; LPD; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTQQFDYDLVIIGAGVGGHGAALHAVDCGLKTAIIEAADMGGTCVNRGCIPSKALLAASGRVRELRDQHHLQSLGIQLGQ
VNFDRGQIAAHADNLVDTIRGNLTNSLTRLKVEIIHGWGKVIGNQKVVVKSDAGEQTITSRDIIIASGSVPWVPPGIEID
GRTVFTSDDAIRLSWLPDWVAIIGSGYIGLEFSDVYTALGSEVTIIEALDTLMPTFDPDIAKIAKRVLIDPRDIDTHAGR
LAKKVTPGSPVVIELADVKTKEVVEVLEVDACLVATGRIPATDNLGLEAISVDTDRRGFIPVNDRMQVLSQGEVVPHVYA
IGDATGKMMLAHAASAQGIVVVENICERPRDVNYRSIPAAAFTHPEISFVGLTEPQAKELAKTEGFKINTVRSYFKANSK
ALAESEADGLAKLIYREDTGEILGGHIIGLHAADLIHEVSNAVAQGQPVQSLSHLVHTHPTISEVIDEAFKRAATGFAH

Sequences:

>Translated_479_residues
MTQQFDYDLVIIGAGVGGHGAALHAVDCGLKTAIIEAADMGGTCVNRGCIPSKALLAASGRVRELRDQHHLQSLGIQLGQ
VNFDRGQIAAHADNLVDTIRGNLTNSLTRLKVEIIHGWGKVIGNQKVVVKSDAGEQTITSRDIIIASGSVPWVPPGIEID
GRTVFTSDDAIRLSWLPDWVAIIGSGYIGLEFSDVYTALGSEVTIIEALDTLMPTFDPDIAKIAKRVLIDPRDIDTHAGR
LAKKVTPGSPVVIELADVKTKEVVEVLEVDACLVATGRIPATDNLGLEAISVDTDRRGFIPVNDRMQVLSQGEVVPHVYA
IGDATGKMMLAHAASAQGIVVVENICERPRDVNYRSIPAAAFTHPEISFVGLTEPQAKELAKTEGFKINTVRSYFKANSK
ALAESEADGLAKLIYREDTGEILGGHIIGLHAADLIHEVSNAVAQGQPVQSLSHLVHTHPTISEVIDEAFKRAATGFAH
>Mature_478_residues
TQQFDYDLVIIGAGVGGHGAALHAVDCGLKTAIIEAADMGGTCVNRGCIPSKALLAASGRVRELRDQHHLQSLGIQLGQV
NFDRGQIAAHADNLVDTIRGNLTNSLTRLKVEIIHGWGKVIGNQKVVVKSDAGEQTITSRDIIIASGSVPWVPPGIEIDG
RTVFTSDDAIRLSWLPDWVAIIGSGYIGLEFSDVYTALGSEVTIIEALDTLMPTFDPDIAKIAKRVLIDPRDIDTHAGRL
AKKVTPGSPVVIELADVKTKEVVEVLEVDACLVATGRIPATDNLGLEAISVDTDRRGFIPVNDRMQVLSQGEVVPHVYAI
GDATGKMMLAHAASAQGIVVVENICERPRDVNYRSIPAAAFTHPEISFVGLTEPQAKELAKTEGFKINTVRSYFKANSKA
LAESEADGLAKLIYREDTGEILGGHIIGLHAADLIHEVSNAVAQGQPVQSLSHLVHTHPTISEVIDEAFKRAATGFAH

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:
GO:0004148: Dihydrolipoyl dehydrogenase
GO:0005737: Dihydrolipoyl dehydrogenase
GO:0016491: Dihydrolipoyl dehydrogenase
GO:0016668: Dihydrolipoyl dehydrogenase
GO:0045454: Dihydrolipoyl dehydrogenase
GO:0050660: Dihydrolipoyl dehydrogenase
GO:0055114: Dihydrolipoyl dehydrogenase

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=480, Percent_Identity=35.4166666666667, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI50301238, Length=468, Percent_Identity=28.4188034188034, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI291045266, Length=488, Percent_Identity=27.8688524590164, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI148277065, Length=493, Percent_Identity=26.369168356998, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI148277071, Length=493, Percent_Identity=26.369168356998, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI33519430, Length=493, Percent_Identity=26.369168356998, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI33519428, Length=493, Percent_Identity=26.369168356998, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI33519426, Length=493, Percent_Identity=26.369168356998, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI22035672, Length=487, Percent_Identity=26.8993839835729, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI291045268, Length=489, Percent_Identity=25.9713701431493, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786307, Length=473, Percent_Identity=33.1923890063425, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87082354, Length=481, Percent_Identity=29.5218295218295, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789915, Length=445, Percent_Identity=30.561797752809, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87081717, Length=479, Percent_Identity=29.4363256784969, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=476, Percent_Identity=35.2941176470588, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI17557007, Length=484, Percent_Identity=25.4132231404959, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=471, Percent_Identity=27.8131634819533, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=471, Percent_Identity=27.8131634819533, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=501, Percent_Identity=25.3493013972056, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=33.5416666666667, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6325166, Length=472, Percent_Identity=27.5423728813559, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325240, Length=482, Percent_Identity=28.6307053941909, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358499, Length=481, Percent_Identity=37.2141372141372, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI24640549, Length=487, Percent_Identity=27.1047227926078, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=27.1047227926078, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=27.1047227926078, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI17737741, Length=493, Percent_Identity=25.1521298174442, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51210; Mature: 51079

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQFDYDLVIIGAGVGGHGAALHAVDCGLKTAIIEAADMGGTCVNRGCIPSKALLAASG
CCCCCCCCEEEEECCCCCCCCEEEEHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC
RVRELRDQHHLQSLGIQLGQVNFDRGQIAAHADNLVDTIRGNLTNSLTRLKVEIIHGWGK
HHHHHHHHHHHHHHCCEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHH
VIGNQKVVVKSDAGEQTITSRDIIIASGSVPWVPPGIEIDGRTVFTSDDAIRLSWLPDWV
HHCCCEEEEECCCCCCEECCCCEEEECCCCCCCCCCCEECCEEEEECCCCEEEEECCHHH
AIIGSGYIGLEFSDVYTALGSEVTIIEALDTLMPTFDPDIAKIAKRVLIDPRDIDTHAGR
HHHCCCCEEEEHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHH
LAKKVTPGSPVVIELADVKTKEVVEVLEVDACLVATGRIPATDNLGLEAISVDTDRRGFI
HHHHCCCCCCEEEEEECCCHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEECCCCCCEE
PVNDRMQVLSQGEVVPHVYAIGDATGKMMLAHAASAQGIVVVENICERPRDVNYRSIPAA
ECCHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCEEEEHHHHCCCCCCCCCCCCCH
AFTHPEISFVGLTEPQAKELAKTEGFKINTVRSYFKANSKALAESEADGLAKLIYREDTG
HCCCCCEEEEECCCHHHHHHHHHCCEEEHHHHHHHHCCCHHHHHHHHHHHEEHEEECCCC
EILGGHIIGLHAADLIHEVSNAVAQGQPVQSLSHLVHTHPTISEVIDEAFKRAATGFAH
CCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQQFDYDLVIIGAGVGGHGAALHAVDCGLKTAIIEAADMGGTCVNRGCIPSKALLAASG
CCCCCCCEEEEECCCCCCCCEEEEHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC
RVRELRDQHHLQSLGIQLGQVNFDRGQIAAHADNLVDTIRGNLTNSLTRLKVEIIHGWGK
HHHHHHHHHHHHHHCCEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHH
VIGNQKVVVKSDAGEQTITSRDIIIASGSVPWVPPGIEIDGRTVFTSDDAIRLSWLPDWV
HHCCCEEEEECCCCCCEECCCCEEEECCCCCCCCCCCEECCEEEEECCCCEEEEECCHHH
AIIGSGYIGLEFSDVYTALGSEVTIIEALDTLMPTFDPDIAKIAKRVLIDPRDIDTHAGR
HHHCCCCEEEEHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHH
LAKKVTPGSPVVIELADVKTKEVVEVLEVDACLVATGRIPATDNLGLEAISVDTDRRGFI
HHHHCCCCCCEEEEEECCCHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEECCCCCCEE
PVNDRMQVLSQGEVVPHVYAIGDATGKMMLAHAASAQGIVVVENICERPRDVNYRSIPAA
ECCHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCEEEEHHHHCCCCCCCCCCCCCH
AFTHPEISFVGLTEPQAKELAKTEGFKINTVRSYFKANSKALAESEADGLAKLIYREDTG
HCCCCCEEEEECCCHHHHHHHHHCCEEEHHHHHHHHCCCHHHHHHHHHHHEEHEEECCCC
EILGGHIIGLHAADLIHEVSNAVAQGQPVQSLSHLVHTHPTISEVIDEAFKRAATGFAH
CCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231; 9387233 [H]