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The map label for this gene is ntrB [H]

Identifier: 158336392

GI number: 158336392

Start: 3287204

End: 3288619

Strand: Reverse

Name: ntrB [H]

Synonym: AM1_3254

Alternate gene names: 158336392

Gene position: 3288619-3287204 (Counterclockwise)

Preceding gene: 158336393

Following gene: 158336390

Centisome position: 50.57

GC content: 50.14

Gene sequence:

>1416_bases
ATGCTTTGTCCAGACGCTTTGACCCAGATAGAAGTCTTTCGCACACTGCCTCCAGAACGCCTGGATTGGATCTGCGATCG
CACACAACATATCCACTTAACCCCAGGTGAAATTCTCGTCCGGGAAGGCGAGCCCCCACTGGGCTTTTTTATTCAAGTCA
GCGGTCAAATTACTGTCAGCCGCAGCAGCAATGGGGCAGATATGCCCGTGGGTCGCCATGTGAGTCCATCTTTCTTTGGA
GAAATTCAATGCCTGACGGAAGATTTAGTTCCGGTTACCTTAACGGCTGATACGAATACCGACCTCTATCGACTCAATTG
TCCCGACTTTTTAGAGGTGCTCCATAGCTGCCGTGACTTTGAAAAAAATATCTTTCGCACCATGGAGAAGCGGTTACGCG
GCCTAGAATTTTTTATTCAAAGTCGGGAAAAAATGGCTGCGTTAGGCACACTTTCAGCAGGATTGGCCCATGAGCTCAAC
AACCCGGCAGCCGCTTTAGTACGGGTACTCAAAGACGTGAAACCCGCCATTCTGGAATTGCAGCGGATGAATTTGGTGTA
TGGTCAACACCAAGTAGATGATGAGCATACTCAGCAATGGTTGGCGCTAAGAGATCACGGTTTTAATGCCATTGCCCATC
CCCAAAATGATCCCCTAGTCTTGGGAGATCGAGAAGATGCCCTGACTGACTGGCTAGAGGACTATGGGGTTGACGAAGCT
TGGAAGTTGGCTGAACCACTCGCTGCAGGGGAAGTAGAGCCTGAAACCCTCGAGCAACTCATGGACCGCTGGCGCAATAG
TGATACTGAACTACGGGATATGGGCCTGAGGTGGTTGGCGCTCTCCTTTGACGTCATGGGGATGATCTTCAGAGGTTTAG
ATGGGGCTGAGCGAATCTCGACGCTAGTGCAGTCCATGAAGTCCTATTCCTATATGGATCGGGCCGCGCAGCAAGAGGTG
GATATCCATGATGGTATTGAAGATACACTGCGCTTGTTTGCATTCAAGCTCAAGCATGGCATCAAGGTTGACCGTCACTA
TGACCAAGACCTCCCTAAAATGATGGCTTTTGGCAGTGAGCTGAACCAGGTGTGGACCAATCTATTGGATAACGCCATCG
ACGCTTTAGGGGAAAGCCCTCCCACTTCCGAACCACCTAAAATTACGATTCGGACTTGTCAAAAGAACAGCTGTATCCGA
GTTGAAGTGGAGGATAATGGTCCGGGCATCCCGCCTGAGATTACAACTCGCATTTTGGAACCGTTTTTTACCACCAAACC
CATGGGCAAAGGATCAGGATTGGGACTGGATGTCGTCCGTCGGATCGTTGAAAATCGTCATGGTGGCAGCCTAATGGTAG
AGTCTGCACCGGGAAAGACGTGCTTTGCGATCACGCTGCCGCTTTCTCGATCATAA

Upstream 100 bases:

>100_bases
GGGTGGCCTCTGGCGTGGGTGAAGGCTCTATTTGCGTCCAGTTTGTTCATCGCCATTTAGCCAACCTGTAGCGTATCCCC
AATTTATAGGCAATTAATCC

Downstream 100 bases:

>100_bases
GGTGAAAGGCGGTTTTGCGGATCAGAGTCATTTGATAAAGCACTTTCGGCGGTCTGTGGGGATAACACCGAAGGTCTTGC
GCGAATCTTGTTAGCCATCG

Product: signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLCPDALTQIEVFRTLPPERLDWICDRTQHIHLTPGEILVREGEPPLGFFIQVSGQITVSRSSNGADMPVGRHVSPSFFG
EIQCLTEDLVPVTLTADTNTDLYRLNCPDFLEVLHSCRDFEKNIFRTMEKRLRGLEFFIQSREKMAALGTLSAGLAHELN
NPAAALVRVLKDVKPAILELQRMNLVYGQHQVDDEHTQQWLALRDHGFNAIAHPQNDPLVLGDREDALTDWLEDYGVDEA
WKLAEPLAAGEVEPETLEQLMDRWRNSDTELRDMGLRWLALSFDVMGMIFRGLDGAERISTLVQSMKSYSYMDRAAQQEV
DIHDGIEDTLRLFAFKLKHGIKVDRHYDQDLPKMMAFGSELNQVWTNLLDNAIDALGESPPTSEPPKITIRTCQKNSCIR
VEVEDNGPGIPPEITTRILEPFFTTKPMGKGSGLGLDVVRRIVENRHGGSLMVESAPGKTCFAITLPLSRS

Sequences:

>Translated_471_residues
MLCPDALTQIEVFRTLPPERLDWICDRTQHIHLTPGEILVREGEPPLGFFIQVSGQITVSRSSNGADMPVGRHVSPSFFG
EIQCLTEDLVPVTLTADTNTDLYRLNCPDFLEVLHSCRDFEKNIFRTMEKRLRGLEFFIQSREKMAALGTLSAGLAHELN
NPAAALVRVLKDVKPAILELQRMNLVYGQHQVDDEHTQQWLALRDHGFNAIAHPQNDPLVLGDREDALTDWLEDYGVDEA
WKLAEPLAAGEVEPETLEQLMDRWRNSDTELRDMGLRWLALSFDVMGMIFRGLDGAERISTLVQSMKSYSYMDRAAQQEV
DIHDGIEDTLRLFAFKLKHGIKVDRHYDQDLPKMMAFGSELNQVWTNLLDNAIDALGESPPTSEPPKITIRTCQKNSCIR
VEVEDNGPGIPPEITTRILEPFFTTKPMGKGSGLGLDVVRRIVENRHGGSLMVESAPGKTCFAITLPLSRS
>Mature_471_residues
MLCPDALTQIEVFRTLPPERLDWICDRTQHIHLTPGEILVREGEPPLGFFIQVSGQITVSRSSNGADMPVGRHVSPSFFG
EIQCLTEDLVPVTLTADTNTDLYRLNCPDFLEVLHSCRDFEKNIFRTMEKRLRGLEFFIQSREKMAALGTLSAGLAHELN
NPAAALVRVLKDVKPAILELQRMNLVYGQHQVDDEHTQQWLALRDHGFNAIAHPQNDPLVLGDREDALTDWLEDYGVDEA
WKLAEPLAAGEVEPETLEQLMDRWRNSDTELRDMGLRWLALSFDVMGMIFRGLDGAERISTLVQSMKSYSYMDRAAQQEV
DIHDGIEDTLRLFAFKLKHGIKVDRHYDQDLPKMMAFGSELNQVWTNLLDNAIDALGESPPTSEPPKITIRTCQKNSCIR
VEVEDNGPGIPPEITTRILEPFFTTKPMGKGSGLGLDVVRRIVENRHGGSLMVESAPGKTCFAITLPLSRS

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=154, Percent_Identity=30.5194805194805, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790436, Length=176, Percent_Identity=31.8181818181818, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788549, Length=189, Percent_Identity=30.6878306878307, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081816, Length=185, Percent_Identity=30.2702702702703, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53014; Mature: 53014

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCPDALTQIEVFRTLPPERLDWICDRTQHIHLTPGEILVREGEPPLGFFIQVSGQITVS
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCEEEECCCCCEEEEEEEECEEEEE
RSSNGADMPVGRHVSPSFFGEIQCLTEDLVPVTLTADTNTDLYRLNCPDFLEVLHSCRDF
ECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHH
EKNIFRTMEKRLRGLEFFIQSREKMAALGTLSAGLAHELNNPAAALVRVLKDVKPAILEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QRMNLVYGQHQVDDEHTQQWLALRDHGFNAIAHPQNDPLVLGDREDALTDWLEDYGVDEA
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCHH
WKLAEPLAAGEVEPETLEQLMDRWRNSDTELRDMGLRWLALSFDVMGMIFRGLDGAERIS
HHHHCCHHCCCCCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHH
TLVQSMKSYSYMDRAAQQEVDIHDGIEDTLRLFAFKLKHGIKVDRHYDQDLPKMMAFGSE
HHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LNQVWTNLLDNAIDALGESPPTSEPPKITIRTCQKNSCIRVEVEDNGPGIPPEITTRILE
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHH
PFFTTKPMGKGSGLGLDVVRRIVENRHGGSLMVESAPGKTCFAITLPLSRS
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCC
>Mature Secondary Structure
MLCPDALTQIEVFRTLPPERLDWICDRTQHIHLTPGEILVREGEPPLGFFIQVSGQITVS
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCEEEECCCCCEEEEEEEECEEEEE
RSSNGADMPVGRHVSPSFFGEIQCLTEDLVPVTLTADTNTDLYRLNCPDFLEVLHSCRDF
ECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHH
EKNIFRTMEKRLRGLEFFIQSREKMAALGTLSAGLAHELNNPAAALVRVLKDVKPAILEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QRMNLVYGQHQVDDEHTQQWLALRDHGFNAIAHPQNDPLVLGDREDALTDWLEDYGVDEA
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCHH
WKLAEPLAAGEVEPETLEQLMDRWRNSDTELRDMGLRWLALSFDVMGMIFRGLDGAERIS
HHHHCCHHCCCCCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHH
TLVQSMKSYSYMDRAAQQEVDIHDGIEDTLRLFAFKLKHGIKVDRHYDQDLPKMMAFGSE
HHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LNQVWTNLLDNAIDALGESPPTSEPPKITIRTCQKNSCIRVEVEDNGPGIPPEITTRILE
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHH
PFFTTKPMGKGSGLGLDVVRRIVENRHGGSLMVESAPGKTCFAITLPLSRS
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]