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The map label for this gene is lepB [H]

Identifier: 158336278

GI number: 158336278

Start: 3167385

End: 3168059

Strand: Reverse

Name: lepB [H]

Synonym: AM1_3140

Alternate gene names: 158336278

Gene position: 3168059-3167385 (Counterclockwise)

Preceding gene: 158336279

Following gene: 158336274

Centisome position: 48.71

GC content: 47.85

Gene sequence:

>675_bases
ATGACGCAACCCCAACCCCCTGATCTGAAACCAACGGAACAACCAGAAGAATCTTGGTGGGTGGAAGCAGCCAAGACGGT
CGGGTTAAGTCTACTTTTGGCATTTGGAATTCGCACCTTTGTCGCGGAAGCCCGCTTTATTCCATCCGGGTCGATGGAAC
CCACTTTGCAAATTCATGATCGTTTGATCATTGATAAAGTCACCTATCGCTTTCGGGATCCTGAACGGGGTGATGTAGTG
GTGTTCAATCCTACTGAATCCTTAAAGCGAGCCAATTTTAAAGAAGCGTTTATTAAACGGGTCGTGGGTGTCCCCGGTGA
TCAAGTTGAAATCAAAAATGGGGTGGTTTGGGTCAACAATCAGCCCATTAAAGAAAATTACACCGCTGAAGGCGTCACTG
TTGGACCTAGCGAAGACCACTGCCGTAACAATTATGTCACTTCAGATGTCGATAGCCAGCCCATTGAACCGCCCGTTCCT
ATCTTTTTAGAAAAGCCTCAAACGATTCCAGACAAGCATTACCTTGTTTTAGGCGATAACCGAGGCAATAGCTATGATGG
TCGCTGTTGGGGATTAGTTGCCCATGCCGATCTAGTAGGAAGAGCTGTCTTTCGTTTTCTGCCCTTTGATCGCATTGGCA
CTTTACCGCCCGCGGAAGTGACCATCGGCGAATAG

Upstream 100 bases:

>100_bases
AGCGCTGACCAGACAGGATTGTGTTATAAATCAACTTACTGGGAAAACTGATTTGTAAGACGTAACCCATCCCTATCCTT
CAAGGCCTGTACCTATCTTT

Downstream 100 bases:

>100_bases
GAATTAAGACACGAGCTGACTATCTTGGCGGAGGCGAAATAGCAAGAACTGAGTTGGTTCATTCGGCTCAAAATTGTTGT
CGCTGACTAGAACTAGAGAC

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I-2; Leader peptidase I-2 [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTQPQPPDLKPTEQPEESWWVEAAKTVGLSLLLAFGIRTFVAEARFIPSGSMEPTLQIHDRLIIDKVTYRFRDPERGDVV
VFNPTESLKRANFKEAFIKRVVGVPGDQVEIKNGVVWVNNQPIKENYTAEGVTVGPSEDHCRNNYVTSDVDSQPIEPPVP
IFLEKPQTIPDKHYLVLGDNRGNSYDGRCWGLVAHADLVGRAVFRFLPFDRIGTLPPAEVTIGE

Sequences:

>Translated_224_residues
MTQPQPPDLKPTEQPEESWWVEAAKTVGLSLLLAFGIRTFVAEARFIPSGSMEPTLQIHDRLIIDKVTYRFRDPERGDVV
VFNPTESLKRANFKEAFIKRVVGVPGDQVEIKNGVVWVNNQPIKENYTAEGVTVGPSEDHCRNNYVTSDVDSQPIEPPVP
IFLEKPQTIPDKHYLVLGDNRGNSYDGRCWGLVAHADLVGRAVFRFLPFDRIGTLPPAEVTIGE
>Mature_223_residues
TQPQPPDLKPTEQPEESWWVEAAKTVGLSLLLAFGIRTFVAEARFIPSGSMEPTLQIHDRLIIDKVTYRFRDPERGDVVV
FNPTESLKRANFKEAFIKRVVGVPGDQVEIKNGVVWVNNQPIKENYTAEGVTVGPSEDHCRNNYVTSDVDSQPIEPPVPI
FLEKPQTIPDKHYLVLGDNRGNSYDGRCWGLVAHADLVGRAVFRFLPFDRIGTLPPAEVTIGE

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=254, Percent_Identity=29.5275590551181, Blast_Score=87, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 25107; Mature: 24976

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPQPPDLKPTEQPEESWWVEAAKTVGLSLLLAFGIRTFVAEARFIPSGSMEPTLQIHD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
RLIIDKVTYRFRDPERGDVVVFNPTESLKRANFKEAFIKRVVGVPGDQVEIKNGVVWVNN
HHHEEHHHHEECCCCCCCEEEECCHHHHHHCCHHHHHHHHHHCCCCCEEEEECCEEEECC
QPIKENYTAEGVTVGPSEDHCRNNYVTSDVDSQPIEPPVPIFLEKPQTIPDKHYLVLGDN
CCCCCCCCCCCEEECCCHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECC
RGNSYDGRCWGLVAHADLVGRAVFRFLPFDRIGTLPPAEVTIGE
CCCCCCCEEEEEEHHHHHHHHHHHHHCCHHHHCCCCCCEEEECC
>Mature Secondary Structure 
TQPQPPDLKPTEQPEESWWVEAAKTVGLSLLLAFGIRTFVAEARFIPSGSMEPTLQIHD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
RLIIDKVTYRFRDPERGDVVVFNPTESLKRANFKEAFIKRVVGVPGDQVEIKNGVVWVNN
HHHEEHHHHEECCCCCCCEEEECCHHHHHHCCHHHHHHHHHHCCCCCEEEEECCEEEECC
QPIKENYTAEGVTVGPSEDHCRNNYVTSDVDSQPIEPPVPIFLEKPQTIPDKHYLVLGDN
CCCCCCCCCCCEEECCCHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECC
RGNSYDGRCWGLVAHADLVGRAVFRFLPFDRIGTLPPAEVTIGE
CCCCCCCEEEEEEHHHHHHHHHHHHHCCHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]