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The map label for this gene is lipB [H]

Identifier: 158336226

GI number: 158336226

Start: 3119260

End: 3119988

Strand: Direct

Name: lipB [H]

Synonym: AM1_3088

Alternate gene names: 158336226

Gene position: 3119260-3119988 (Clockwise)

Preceding gene: 158336221

Following gene: 158336227

Centisome position: 47.96

GC content: 50.21

Gene sequence:

>729_bases
TTGATTTCATCTCACCAATCGGTTAAGGATTCGGACTCATCTCTCCCTAAACGTCCCTGTTGGCTGTACCAACTGGGTCT
GGTGGACTACCAAAAAGCGTGGGATTGGCAGCGGCAACTGGTGTGCGATCGCATTCACAATCCTCAACTTCCCGATACAT
TACTCCTGTTAGAGCATCCCCCGGTCTATACCCTGGGACAGGGTGCAAGTTTAGACTTTCTTAAATTCAATCCTGACCAA
TCAGACTTTGCCCTGCACCGGATCGAACGCGGAGGAGAAGTCACTTATCACTGTCCGGGTCAACTCATCGGCTATCCCAT
TCTCAATCTCAAGCACCATCAAACTGACCTGCACTGGTATCTACGGCAGCTAGAAGAAGTAATCATTCGTACCCTGGGCA
TTTACGGACTAGAAGCAGAGCGTATTCCGGGCCTTACAGGCGTCTGGCTAGACCAGTGCAAAGTCGCAGCCATCGGCATT
AAGGTCAGTCGCTGGATTACCATGCACGGCTTTTCTCTCAATATCAACCCCGATCTGTCTGGCTTTGACCAGATCATTCC
CTGTGGTATCCATAATCGGGCCGTCGGCAGCTTAGAGCAGTTTATTCCCAACCTAGACAGTGCTGAGGTCACTGCCACAC
TGCTGCATCAGTTTTCCCGGGTGTTCGATCTAGATTTGAGACGAGAGGGCCCTGAACCTTTTATCTGTCATCCTTCATCG
CTTGATTAA

Upstream 100 bases:

>100_bases
GTTTTCTGCAAGGGGGGGCTGTTCTACCGTGTCTTCAACTTGCTTTGCATCCCGTGATAATTCTTAAATGCTATCGTCAT
AGGATTACTCGGAAATACCC

Downstream 100 bases:

>100_bases
GTCATTACGATAATCCGTCATGATAGAGAAGCAGTGCCCTTCTCCCGCCATGACTTCCTCTGTTGCTTCTACAGACGATC
ACTTTCTGATTACATTTGCT

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MISSHQSVKDSDSSLPKRPCWLYQLGLVDYQKAWDWQRQLVCDRIHNPQLPDTLLLLEHPPVYTLGQGASLDFLKFNPDQ
SDFALHRIERGGEVTYHCPGQLIGYPILNLKHHQTDLHWYLRQLEEVIIRTLGIYGLEAERIPGLTGVWLDQCKVAAIGI
KVSRWITMHGFSLNINPDLSGFDQIIPCGIHNRAVGSLEQFIPNLDSAEVTATLLHQFSRVFDLDLRREGPEPFICHPSS
LD

Sequences:

>Translated_242_residues
MISSHQSVKDSDSSLPKRPCWLYQLGLVDYQKAWDWQRQLVCDRIHNPQLPDTLLLLEHPPVYTLGQGASLDFLKFNPDQ
SDFALHRIERGGEVTYHCPGQLIGYPILNLKHHQTDLHWYLRQLEEVIIRTLGIYGLEAERIPGLTGVWLDQCKVAAIGI
KVSRWITMHGFSLNINPDLSGFDQIIPCGIHNRAVGSLEQFIPNLDSAEVTATLLHQFSRVFDLDLRREGPEPFICHPSS
LD
>Mature_242_residues
MISSHQSVKDSDSSLPKRPCWLYQLGLVDYQKAWDWQRQLVCDRIHNPQLPDTLLLLEHPPVYTLGQGASLDFLKFNPDQ
SDFALHRIERGGEVTYHCPGQLIGYPILNLKHHQTDLHWYLRQLEEVIIRTLGIYGLEAERIPGLTGVWLDQCKVAAIGI
KVSRWITMHGFSLNINPDLSGFDQIIPCGIHNRAVGSLEQFIPNLDSAEVTATLLHQFSRVFDLDLRREGPEPFICHPSS
LD

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:
GO:0003824: Octanoyltransferase
GO:0005737: Octanoyltransferase
GO:0006464: Octanoyltransferase
GO:0009107: Octanoyltransferase
GO:0016415: Octanoyltransferase
GO:0016740: Octanoyltransferase
GO:0016746: Octanoyltransferase
GO:0016874: Octanoyltransferase
GO:0033819: Octanoyltransferase

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=220, Percent_Identity=34.0909090909091, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI87081767, Length=195, Percent_Identity=30.7692307692308, Blast_Score=104, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323268, Length=197, Percent_Identity=28.9340101522843, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355483, Length=205, Percent_Identity=35.609756097561, Blast_Score=131, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 27582; Mature: 27582

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSHQSVKDSDSSLPKRPCWLYQLGLVDYQKAWDWQRQLVCDRIHNPQLPDTLLLLEHP
CCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
PVYTLGQGASLDFLKFNPDQSDFALHRIERGGEVTYHCPGQLIGYPILNLKHHQTDLHWY
CEEEECCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCEECCEEECCCCCHHHHHHH
LRQLEEVIIRTLGIYGLEAERIPGLTGVWLDQCKVAAIGIKVSRWITMHGFSLNINPDLS
HHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHCCEEEEEEEEEEEEEEEECEEEEECCCCC
GFDQIIPCGIHNRAVGSLEQFIPNLDSAEVTATLLHQFSRVFDLDLRREGPEPFICHPSS
CCHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
LD
CC
>Mature Secondary Structure
MISSHQSVKDSDSSLPKRPCWLYQLGLVDYQKAWDWQRQLVCDRIHNPQLPDTLLLLEHP
CCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
PVYTLGQGASLDFLKFNPDQSDFALHRIERGGEVTYHCPGQLIGYPILNLKHHQTDLHWY
CEEEECCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCEECCEEECCCCCHHHHHHH
LRQLEEVIIRTLGIYGLEAERIPGLTGVWLDQCKVAAIGIKVSRWITMHGFSLNINPDLS
HHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHCCEEEEEEEEEEEEEEEECEEEEECCCCC
GFDQIIPCGIHNRAVGSLEQFIPNLDSAEVTATLLHQFSRVFDLDLRREGPEPFICHPSS
CCHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
LD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA