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The map label for this gene is rbfA

Identifier: 158336219

GI number: 158336219

Start: 3109209

End: 3109625

Strand: Direct

Name: rbfA

Synonym: AM1_3081

Alternate gene names: 158336219

Gene position: 3109209-3109625 (Clockwise)

Preceding gene: 158336218

Following gene: 158336220

Centisome position: 47.81

GC content: 47.48

Gene sequence:

>417_bases
ATGGCTACAGGACGACGGGTTTCTCGTGTTGCCGAATTAATTAAACGCGAAGTCAGCCAAATGTTATTGCATAGCATCAA
AGATGATCGAGTCGGATCCGGGATGATTAGCGTTACGGCTGTGGATCTATCGGGTGATCTCCAACATGCCAAGATTTTTG
TCAGCATTTATGGCACTGATGAAGCTAGAGCAGAAACAATGGCCGGTCTAGAATCAGCCACAGGCTATGTTCGTAAACAA
TTGGGGCATCGCATCCACCTTCGCCGCACTCCAGAAGTCATTTTTTGTGAAGATCGCTCCTTTGAAGAAGGCAATCAGGT
ACTGTCGTTGCTCCATAAACTCGATCATGAGCGCCAGAATAAGCCTGCTGCCTCAACCGAAAAGCCTCCTGTAGGATCTT
TAGACGCTGATCTCTAG

Upstream 100 bases:

>100_bases
CTCAGTGCCATGATGGGGATGACTCCATTAAACTAGGCCTTGGGGAGACAACATATCTGTTAGCGGTTTCATCTCAGGGG
AGATGGAGGAGTAGAAACGA

Downstream 100 bases:

>100_bases
TTGGATGAACTCTTGACTCATTTATTGCCAGAGCCAGATTCTCTGTCTTTGCCAGAACAAGTTGCTCAAATGTTGGTCGT
CCGAGCCTCCGGCTATCTGT

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 138; Mature: 137

Protein sequence:

>138_residues
MATGRRVSRVAELIKREVSQMLLHSIKDDRVGSGMISVTAVDLSGDLQHAKIFVSIYGTDEARAETMAGLESATGYVRKQ
LGHRIHLRRTPEVIFCEDRSFEEGNQVLSLLHKLDHERQNKPAASTEKPPVGSLDADL

Sequences:

>Translated_138_residues
MATGRRVSRVAELIKREVSQMLLHSIKDDRVGSGMISVTAVDLSGDLQHAKIFVSIYGTDEARAETMAGLESATGYVRKQ
LGHRIHLRRTPEVIFCEDRSFEEGNQVLSLLHKLDHERQNKPAASTEKPPVGSLDADL
>Mature_137_residues
ATGRRVSRVAELIKREVSQMLLHSIKDDRVGSGMISVTAVDLSGDLQHAKIFVSIYGTDEARAETMAGLESATGYVRKQL
GHRIHLRRTPEVIFCEDRSFEEGNQVLSLLHKLDHERQNKPAASTEKPPVGSLDADL

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family

Homologues:

Organism=Escherichia coli, GI1789558, Length=127, Percent_Identity=34.6456692913386, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RBFA_ACAM1 (B0CDK8)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517393.1
- ProteinModelPortal:   B0CDK8
- GeneID:   5681889
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3081
- HOGENOM:   HBG751055
- OMA:   SIEYGNR
- BioCyc:   AMAR329726:AM1_3081-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00003
- InterPro:   IPR015946
- InterPro:   IPR000238
- InterPro:   IPR020053
- Gene3D:   G3DSA:3.30.300.20
- ProDom:   PD007327
- TIGRFAMs:   TIGR00082

Pfam domain/function: PF02033 RBFA; SSF89919 Rib_bd_factA

EC number: NA

Molecular weight: Translated: 15294; Mature: 15162

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGRRVSRVAELIKREVSQMLLHSIKDDRVGSGMISVTAVDLSGDLQHAKIFVSIYGTD
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCC
EARAETMAGLESATGYVRKQLGHRIHLRRTPEVIFCEDRSFEEGNQVLSLLHKLDHERQN
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCC
KPAASTEKPPVGSLDADL
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATGRRVSRVAELIKREVSQMLLHSIKDDRVGSGMISVTAVDLSGDLQHAKIFVSIYGTD
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCC
EARAETMAGLESATGYVRKQLGHRIHLRRTPEVIFCEDRSFEEGNQVLSLLHKLDHERQN
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCC
KPAASTEKPPVGSLDADL
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA