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The map label for this gene is thiC [H]

Identifier: 158336140

GI number: 158336140

Start: 3031210

End: 3032586

Strand: Reverse

Name: thiC [H]

Synonym: AM1_3002

Alternate gene names: 158336140

Gene position: 3032586-3031210 (Counterclockwise)

Preceding gene: 158336145

Following gene: 158336139

Centisome position: 46.63

GC content: 50.47

Gene sequence:

>1377_bases
ATGCGTCATCAATGGGTTGCTAACCGTCGCGGACAGAGCAATGTCAGTCAGATGCACTATGCGCGACAGGGCCAAATCAC
GGAAGAAATGGACTATGTTGCCAAACGTGAGAATCTTCCAGCTGATTTGATTCGAGAAGAAGTGGCTCGGGGACGGATGA
TCATTCCGGCCAACGTGAATCACGTTAATTTGGAACCGATGGCCATTGGTATTGCGTCCAAATGTAAGGTGAATGCCAAT
ATTGGTGCTTCTCCTAACTCGTCCAATATTGAAGAAGAACTCGACAAGCTCCGCTTAGCGGTCAAGTACGGAGCCGATAC
GGTTATGGATTTGTCGACAGGCGGCGGCAATTTAGATGAAATTCGCACTGCCATTATCAATGAATCTTCAGTACCCATTG
GCACAGTGCCTATTTATCAAGCTCTAGAAAGTGTGCATGGCAACATTGAGAATCTAACGGCTGATGACTTTTTGCACATC
ATTGACAAGCATGCCCAGCAAGGAGTGGACTACCAAACCATTCATGCTGGCATTTTGATTGAGCATTTGCCCTTGGTGCG
CGATCGCATCACTGGTATCGTCTCCCGTGGCGGAGGTATTCTGGCCCGCTGGATGCTGCATCATCATAAGCAAAATCCAC
TGTATACCCGCTTCAATGACGTTATCGAAATCTTCAAAAAGCATGATGTGTCTTTTAGCTTGGGCGATTCCCTACGCCCT
GGCTGTACCCACGATGCCTCTGATGATGCCCAGCTGGCAGAGCTGAAGACCTTAGGACAACTCACCCGCCGAGCTTGGGA
ACATGATGTTCAGGTGATGGTGGAAGGTCCAGGTCATGTGCCCATGGACCAAATTGAATTCAATGTTCGCAAGCAAATGG
AGGAATGCTCGGAAGCACCTTTCTATGTGTTGGGACCATTGGTCACTGATATTGCGCCAGGGTATGACCACATTACGTCT
GCGATTGGGGCCGCGATGGCAGGTTGGTATGGCACCGCCATGCTTTGCTATGTGACTCCTAAAGAACATCTAGGCCTGCC
GGATGCAGAGGATGTGCGCAATGGCCTCATTGCTTATAAGATTGCTGCCCATGCCGCAGATATTGCTCGCCATCGTCCGG
GGGCTCGCGATCGAGATGATCAGCTTTCAGAAGCCCGCTATAACTTCGACTGGAATCGTCAGTTTGAGCTGTCCCTCGAT
CCGGAGCGGGCTCGTGAATACCACGATGAGACGCTACCCGCCGATATCTACAAGACGGCCGAATTCTGCTCCATGTGTGG
ACCGAAGTTCTGCCCCATGCAAACCAAGGTCGATGCGGATGCCTTAACTGAACTGGAGAAGTTCTTAGCTAAGGATCAAG
TGGGTTCTGCCCGTTAG

Upstream 100 bases:

>100_bases
GTGTCAGCTAGGGGTGCTGTATTACGGCTGAGATCAAACCCTTAGAACCTGAGACCGGCTAGCACCGGCGGAGGGAAGCT
GTTCATCCGAGGAACTTAAT

Downstream 100 bases:

>100_bases
GAGAAAAAGAGGTGAACGGTAAAGATTGGCAGTCTTATAGAGTGAAAGGGTGGGGTATTTGTCTGGCACTTTTGCTCTGC
TGCTGGTCTTTACCGGGGCT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRHQWVANRRGQSNVSQMHYARQGQITEEMDYVAKRENLPADLIREEVARGRMIIPANVNHVNLEPMAIGIASKCKVNAN
IGASPNSSNIEEELDKLRLAVKYGADTVMDLSTGGGNLDEIRTAIINESSVPIGTVPIYQALESVHGNIENLTADDFLHI
IDKHAQQGVDYQTIHAGILIEHLPLVRDRITGIVSRGGGILARWMLHHHKQNPLYTRFNDVIEIFKKHDVSFSLGDSLRP
GCTHDASDDAQLAELKTLGQLTRRAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDIAPGYDHITS
AIGAAMAGWYGTAMLCYVTPKEHLGLPDAEDVRNGLIAYKIAAHAADIARHRPGARDRDDQLSEARYNFDWNRQFELSLD
PERAREYHDETLPADIYKTAEFCSMCGPKFCPMQTKVDADALTELEKFLAKDQVGSAR

Sequences:

>Translated_458_residues
MRHQWVANRRGQSNVSQMHYARQGQITEEMDYVAKRENLPADLIREEVARGRMIIPANVNHVNLEPMAIGIASKCKVNAN
IGASPNSSNIEEELDKLRLAVKYGADTVMDLSTGGGNLDEIRTAIINESSVPIGTVPIYQALESVHGNIENLTADDFLHI
IDKHAQQGVDYQTIHAGILIEHLPLVRDRITGIVSRGGGILARWMLHHHKQNPLYTRFNDVIEIFKKHDVSFSLGDSLRP
GCTHDASDDAQLAELKTLGQLTRRAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDIAPGYDHITS
AIGAAMAGWYGTAMLCYVTPKEHLGLPDAEDVRNGLIAYKIAAHAADIARHRPGARDRDDQLSEARYNFDWNRQFELSLD
PERAREYHDETLPADIYKTAEFCSMCGPKFCPMQTKVDADALTELEKFLAKDQVGSAR
>Mature_458_residues
MRHQWVANRRGQSNVSQMHYARQGQITEEMDYVAKRENLPADLIREEVARGRMIIPANVNHVNLEPMAIGIASKCKVNAN
IGASPNSSNIEEELDKLRLAVKYGADTVMDLSTGGGNLDEIRTAIINESSVPIGTVPIYQALESVHGNIENLTADDFLHI
IDKHAQQGVDYQTIHAGILIEHLPLVRDRITGIVSRGGGILARWMLHHHKQNPLYTRFNDVIEIFKKHDVSFSLGDSLRP
GCTHDASDDAQLAELKTLGQLTRRAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDIAPGYDHITS
AIGAAMAGWYGTAMLCYVTPKEHLGLPDAEDVRNGLIAYKIAAHAADIARHRPGARDRDDQLSEARYNFDWNRQFELSLD
PERAREYHDETLPADIYKTAEFCSMCGPKFCPMQTKVDADALTELEKFLAKDQVGSAR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=447, Percent_Identity=57.9418344519016, Blast_Score=531, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 51137; Mature: 51137

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHQWVANRRGQSNVSQMHYARQGQITEEMDYVAKRENLPADLIREEVARGRMIIPANVN
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HVNLEPMAIGIASKCKVNANIGASPNSSNIEEELDKLRLAVKYGADTVMDLSTGGGNLDE
EEECCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
IRTAIINESSVPIGTVPIYQALESVHGNIENLTADDFLHIIDKHAQQGVDYQTIHAGILI
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
EHLPLVRDRITGIVSRGGGILARWMLHHHKQNPLYTRFNDVIEIFKKHDVSFSLGDSLRP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCC
GCTHDASDDAQLAELKTLGQLTRRAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECSEAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDIAPGYDHITSAIGAAMAGWYGTAMLCYVTPKEHLGLPDAEDVRNGLIAYK
EEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCCEEEH
IAAHAADIARHRPGARDRDDQLSEARYNFDWNRQFELSLDPERAREYHDETLPADIYKTA
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHH
EFCSMCGPKFCPMQTKVDADALTELEKFLAKDQVGSAR
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRHQWVANRRGQSNVSQMHYARQGQITEEMDYVAKRENLPADLIREEVARGRMIIPANVN
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HVNLEPMAIGIASKCKVNANIGASPNSSNIEEELDKLRLAVKYGADTVMDLSTGGGNLDE
EEECCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
IRTAIINESSVPIGTVPIYQALESVHGNIENLTADDFLHIIDKHAQQGVDYQTIHAGILI
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
EHLPLVRDRITGIVSRGGGILARWMLHHHKQNPLYTRFNDVIEIFKKHDVSFSLGDSLRP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCC
GCTHDASDDAQLAELKTLGQLTRRAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECSEAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDIAPGYDHITSAIGAAMAGWYGTAMLCYVTPKEHLGLPDAEDVRNGLIAYK
EEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCCEEEH
IAAHAADIARHRPGARDRDDQLSEARYNFDWNRQFELSLDPERAREYHDETLPADIYKTA
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHH
EFCSMCGPKFCPMQTKVDADALTELEKFLAKDQVGSAR
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA