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The map label for this gene is purF [H]

Identifier: 158335762

GI number: 158335762

Start: 2649927

End: 2651435

Strand: Direct

Name: purF [H]

Synonym: AM1_2613

Alternate gene names: 158335762

Gene position: 2649927-2651435 (Clockwise)

Preceding gene: 158335761

Following gene: 158335765

Centisome position: 40.74

GC content: 51.95

Gene sequence:

>1509_bases
ATGTTTCCTGCTGCTTCTGTCCCATCGATTCCCACTGGCAACGATCCCGTTGATCCCCACCTTTCCACCGAATCGTGGTC
CGAAGACAAACCAGAAGAAGCCTGTGGCGTGTTTGGCGTGTTTGCGCCCGGTTTAGAAGCCGCCAAACTAGCTTACTTTG
GTCTCTATGCCCTCCAACATCGAGGTCAAGAATCCGCAGGGATTACCACTTACACCCCAGAGGGGAAAACCCACACCTAC
AAAAACATGGGGTTGGTCTCCCAGGTCTTTTCAGAACCGATTTTGAATGACTTACCCGGCCATATTGCCGTTGGTCATAC
CCGCTACTCCACCACCGGTTCGAGCCGAGTCGCCAATGCCCAACCCGTCGTCGTTGAAACCCCTAAAGGCTCAATCGCCT
TGGCCCATAATGGCAATTTAGTGAATACCGTCGAGTTTCGCAAAACGTTACTGGCCAAAAACTGTCCCCTGGAAACCACC
ACAGATTCTGAAGTGATTGCCTATGCCATTGGCGAAGCAGTCAAAGCGGGCAAGGACTGGTACTCCAGTGTGATTGAAGC
TTGTCAGCAATGCGAAGGGGCCTTCAGTCTAGTGATTGGCACACCGACCGGCATACTCGGCGTTCGCGACCCCCAGGGAA
TTCGCCCTTTGGTCCTCGGTACCATTGACACGGATAATCCCGATAAACCCCATTACGTCCTCGCCTCCGAAACCTGCGCC
CTCGACATCATCGGAGCAACCCACGTTCGTGAAATCGAGCCAGGGGAAATTGTTCATATCACGGCTGACGGCATTACCTC
CGATACCTGGGCTCCTAAGCCTGAGCGAAAATTGTGCATCTTTGAGATGATTTACTTTGCTCGCCCCGATAGCCATATGA
AAGGCGAAAGTCTCTATAGCTACCGCTTGCGTCTGGGTCACCATCTCGGCAAGGAATCTCCCATCGATGCAGATATGGTG
ATTGCAGTCCCCGATTCTGGTGTGCCTGCCGCCATTGGCTATTCCCAAACCAGCGGCATTCCCTATGCCGAAGGATTGAT
TAAAAATCGCTATGTGGGCCGAACCTTTATTCAACCCACCCAAGCCATGCGTGAAATCGGGATTCGCATGAAGCTCAACC
CACTCCGAGATGTCTTGGAAGGGAAGCGGATTATCATTGTCGACGATTCGATTGTTCGAGGCACCACCAGCTACAAAATT
GTCCGTGCCCTCCGCGAAGCAGGCGCCACAGAAGTCCATATGCGAGTCTCTTCTCCTCCCGTGACGCATCCCTGCTTTTA
TGGCATTGACACGGACGATCAAGATCAGCTGATCGCAGCCACCACCTCGACCTCCGATATCGCTCAAAAAATTGGGGTTG
ACTCCCTTCACTATCTCAGTTGGGAAGGTATGTTAGCGGCAACCGGACGTAACCCTGAGAATTATTGTTCAGCCTGCTTT
ACAGGAGATTATCCCATCTCCATTCCTGAACCTCTCAAACGTACAAAACTCATGTTGGAAAAAGCATAA

Upstream 100 bases:

>100_bases
TCCAAGCTGGCGTAAATCCAGGCTGCTTGAGGCTGGAAAGGACAAATGTTTGTAATGTGTCAGTTTTTCTGGTTTATTCC
GTTTACTTAACGACTTAAAT

Downstream 100 bases:

>100_bases
GCCTGTTTCCTTCCAAAGCTAAAGATACTGAAAATGGCCTAGAAGAGGTCATCTAAGCTGTAAGCGTTAACCTTTTAAGC
TTGCACTGATCCCATGGAAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MFPAASVPSIPTGNDPVDPHLSTESWSEDKPEEACGVFGVFAPGLEAAKLAYFGLYALQHRGQESAGITTYTPEGKTHTY
KNMGLVSQVFSEPILNDLPGHIAVGHTRYSTTGSSRVANAQPVVVETPKGSIALAHNGNLVNTVEFRKTLLAKNCPLETT
TDSEVIAYAIGEAVKAGKDWYSSVIEACQQCEGAFSLVIGTPTGILGVRDPQGIRPLVLGTIDTDNPDKPHYVLASETCA
LDIIGATHVREIEPGEIVHITADGITSDTWAPKPERKLCIFEMIYFARPDSHMKGESLYSYRLRLGHHLGKESPIDADMV
IAVPDSGVPAAIGYSQTSGIPYAEGLIKNRYVGRTFIQPTQAMREIGIRMKLNPLRDVLEGKRIIIVDDSIVRGTTSYKI
VRALREAGATEVHMRVSSPPVTHPCFYGIDTDDQDQLIAATTSTSDIAQKIGVDSLHYLSWEGMLAATGRNPENYCSACF
TGDYPISIPEPLKRTKLMLEKA

Sequences:

>Translated_502_residues
MFPAASVPSIPTGNDPVDPHLSTESWSEDKPEEACGVFGVFAPGLEAAKLAYFGLYALQHRGQESAGITTYTPEGKTHTY
KNMGLVSQVFSEPILNDLPGHIAVGHTRYSTTGSSRVANAQPVVVETPKGSIALAHNGNLVNTVEFRKTLLAKNCPLETT
TDSEVIAYAIGEAVKAGKDWYSSVIEACQQCEGAFSLVIGTPTGILGVRDPQGIRPLVLGTIDTDNPDKPHYVLASETCA
LDIIGATHVREIEPGEIVHITADGITSDTWAPKPERKLCIFEMIYFARPDSHMKGESLYSYRLRLGHHLGKESPIDADMV
IAVPDSGVPAAIGYSQTSGIPYAEGLIKNRYVGRTFIQPTQAMREIGIRMKLNPLRDVLEGKRIIIVDDSIVRGTTSYKI
VRALREAGATEVHMRVSSPPVTHPCFYGIDTDDQDQLIAATTSTSDIAQKIGVDSLHYLSWEGMLAATGRNPENYCSACF
TGDYPISIPEPLKRTKLMLEKA
>Mature_502_residues
MFPAASVPSIPTGNDPVDPHLSTESWSEDKPEEACGVFGVFAPGLEAAKLAYFGLYALQHRGQESAGITTYTPEGKTHTY
KNMGLVSQVFSEPILNDLPGHIAVGHTRYSTTGSSRVANAQPVVVETPKGSIALAHNGNLVNTVEFRKTLLAKNCPLETT
TDSEVIAYAIGEAVKAGKDWYSSVIEACQQCEGAFSLVIGTPTGILGVRDPQGIRPLVLGTIDTDNPDKPHYVLASETCA
LDIIGATHVREIEPGEIVHITADGITSDTWAPKPERKLCIFEMIYFARPDSHMKGESLYSYRLRLGHHLGKESPIDADMV
IAVPDSGVPAAIGYSQTSGIPYAEGLIKNRYVGRTFIQPTQAMREIGIRMKLNPLRDVLEGKRIIIVDDSIVRGTTSYKI
VRALREAGATEVHMRVSSPPVTHPCFYGIDTDDQDQLIAATTSTSDIAQKIGVDSLHYLSWEGMLAATGRNPENYCSACF
TGDYPISIPEPLKRTKLMLEKA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:
GO:0004044: Amidophosphoribosyltransferase
GO:0006164: Amidophosphoribosyltransferase
GO:0008152: Amidophosphoribosyltransferase
GO:0009113: Amidophosphoribosyltransferase
GO:0009116: Amidophosphoribosyltransferase
GO:0016740: Amidophosphoribosyltransferase
GO:0016757: Amidophosphoribosyltransferase
GO:0046872: Amidophosphoribosyltransferase
GO:0051536: Amidophosphoribosyltransferase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=508, Percent_Identity=41.3385826771654, Blast_Score=369, Evalue=1e-102,
Organism=Escherichia coli, GI1788651, Length=470, Percent_Identity=37.8723404255319, Blast_Score=304, Evalue=1e-83,
Organism=Escherichia coli, GI1790167, Length=230, Percent_Identity=30, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=40.2922755741127, Blast_Score=334, Evalue=6e-92,
Organism=Saccharomyces cerevisiae, GI6323958, Length=457, Percent_Identity=36.1050328227571, Blast_Score=274, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=42.6229508196721, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24659604, Length=465, Percent_Identity=43.010752688172, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI28573187, Length=517, Percent_Identity=41.1992263056093, Blast_Score=381, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54427; Mature: 54427

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPAASVPSIPTGNDPVDPHLSTESWSEDKPEEACGVFGVFAPGLEAAKLAYFGLYALQH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCEEEECCCCCHHHHHHHHHHHHHHH
RGQESAGITTYTPEGKTHTYKNMGLVSQVFSEPILNDLPGHIAVGHTRYSTTGSSRVANA
CCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECCEEECCCCCCCCCCC
QPVVVETPKGSIALAHNGNLVNTVEFRKTLLAKNCPLETTTDSEVIAYAIGEAVKAGKDW
CCEEEECCCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YSSVIEACQQCEGAFSLVIGTPTGILGVRDPQGIRPLVLGTIDTDNPDKPHYVLASETCA
HHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCCEEEEEEECCCCCCCCCEEEEECCEE
LDIIGATHVREIEPGEIVHITADGITSDTWAPKPERKLCIFEMIYFARPDSHMKGESLYS
EEEECCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHH
YRLRLGHHLGKESPIDADMVIAVPDSGVPAAIGYSQTSGIPYAEGLIKNRYVGRTFIQPT
HHHHHHHHCCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCHHHHHHHCCCCCCHHCCHH
QAMREIGIRMKLNPLRDVLEGKRIIIVDDSIVRGTTSYKIVRALREAGATEVHMRVSSPP
HHHHHCCCEEEEHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCC
VTHPCFYGIDTDDQDQLIAATTSTSDIAQKIGVDSLHYLSWEGMLAATGRNPENYCSACF
CCCCEEECCCCCCCCCEEEEECCHHHHHHHHCCCCEEEEEECCEEEECCCCHHHHHHHEE
TGDYPISIPEPLKRTKLMLEKA
CCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MFPAASVPSIPTGNDPVDPHLSTESWSEDKPEEACGVFGVFAPGLEAAKLAYFGLYALQH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCEEEECCCCCHHHHHHHHHHHHHHH
RGQESAGITTYTPEGKTHTYKNMGLVSQVFSEPILNDLPGHIAVGHTRYSTTGSSRVANA
CCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECCEEECCCCCCCCCCC
QPVVVETPKGSIALAHNGNLVNTVEFRKTLLAKNCPLETTTDSEVIAYAIGEAVKAGKDW
CCEEEECCCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
YSSVIEACQQCEGAFSLVIGTPTGILGVRDPQGIRPLVLGTIDTDNPDKPHYVLASETCA
HHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCCEEEEEEECCCCCCCCCEEEEECCEE
LDIIGATHVREIEPGEIVHITADGITSDTWAPKPERKLCIFEMIYFARPDSHMKGESLYS
EEEECCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHH
YRLRLGHHLGKESPIDADMVIAVPDSGVPAAIGYSQTSGIPYAEGLIKNRYVGRTFIQPT
HHHHHHHHCCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCHHHHHHHCCCCCCHHCCHH
QAMREIGIRMKLNPLRDVLEGKRIIIVDDSIVRGTTSYKIVRALREAGATEVHMRVSSPP
HHHHHCCCEEEEHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCC
VTHPCFYGIDTDDQDQLIAATTSTSDIAQKIGVDSLHYLSWEGMLAATGRNPENYCSACF
CCCCEEECCCCCCCCCEEEEECCHHHHHHHHCCCCEEEEEECCEEEECCCCHHHHHHHEE
TGDYPISIPEPLKRTKLMLEKA
CCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]