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The map label for this gene is nuoL [H]

Identifier: 158335646

GI number: 158335646

Start: 2519790

End: 2521853

Strand: Direct

Name: nuoL [H]

Synonym: AM1_2496

Alternate gene names: 158335646

Gene position: 2519790-2521853 (Clockwise)

Preceding gene: 158335645

Following gene: 158335647

Centisome position: 38.74

GC content: 50.97

Gene sequence:

>2064_bases
ATGGATTCGTTATATCAATTTGCTTGGCTTATCCCTGTATTGCCACTTGGCGGTGCATTTCTCGTAGGTTTGGGGTTAAT
TTCCTATAACAAACAAACCAACGCTCTCCGTCAAATCAACGCGGCCTTTATTGTGTCTTTGATGGGAGCGGCAATGGTCC
TCTCCTTCTCCATCTTATGGAGTCAGATTCAAGGTCACGAGCCCTATCTGATGACCTTTGAATGGGCCTCAGCAGGAGCA
TTTCACCTGACGATGGGCTATACCATCGATCATCTGACCTCCTTGATGTTAGTGGTGGTCACCACTGTTGCCTTCCTGGT
GATGATCTATACCGATGGGTACATGGCTCATGACCCCGGTTATGTAAGGTTTTATGCATATTTAAGTCTATTTAGCTTCT
CCATGTTGGGGCTCGTGGTTAGCCCGAACTTGGTGCAAATTTATATTTTTTGGGAATTGGTAGGGATGTGTTCCTACCTG
CTGGTGGGATTCTGGTACGACCGCAAGCCCGCCGCCAACGCCTGCCAAAAAGCATTTGTAACAAACCGAGTCGGTGACTT
TGGGTTACTGCTCGGTATTTTAGGCCTGTACTGGGCAACGAATAGTTTTGAGTTCGACATTATGGGAGAGCGATTGCAGT
CCCTCGTGTCGACCGGTGAACTCAGCGGGGTGGTGGCTGCCATCTTCGCTATTTTGGTGTTTATGGGACCCATGGCTAAA
TCAGCCCAGTTCCCCCTCCATGTCTGGCTGCCCGAAGCCATGGAAGGTCCGACCCCCATTTCGGCCCTGATTCACGCAGC
GACCATGGTTGCCGCTGGTGTTTTCCTCGTGGCTCGGATGTTCCCTGTATTCTCGGGCATCCCCACCGCCATGCAGTTTA
TTGCCTGGACGGGCGCGATTACCGCCTTCTTAGGCGCTACGATTGCGGTTACTCAAAACGATATTAAGAAGGGTCTAGCC
TACTCCACCATCTCCCAATTGGGCTATATGGTGATGGCTATGGGCATCGGTGCCTACTCCGCAGGTCTATTCCACCTCAT
GACCCATGCTTACTTTAAGGCCATGTTGTTTCTAGGGTCTGGCTCTGTGATCCACGGTATGGAAGCGGTGGTGGGCCATA
ACCCTGCTTTAGCCCAAGATATGCGTCTGATGGGCGGCCTGCGCAAGTACATGCCCTATACCGGCATCACATTTTTAATC
GGTTGCCTAGCGATTTGCGGTATTCCGCCTTTTGCGGGTTTCTGGTCTAAAGACGAGATTCTCAGTTCCGCCTTTGAAGC
CAATCCAGCCTTATGGTTGGTGGGCTGGCTAACGGCAGGTCTGACTGCCTTCTATATGTTCCGGATGTATTTCTCCACCT
TTGAAGGAGACTTCCGGGGTAATATCAATGCTTTGCGTGAGCAGATTGATGGCAAATCTCCAGCCCTCAACTTCGGCCCT
GGCGCCATGGATCCCCGGGAGTTGACTATGGTGGCTGAGCAAGAGGCTCATCATCATGACGATCATCACCATGCGGATGA
ACCCCATGAATCTCCGATTACCATGACCTTGCCGCTCATGGTGTTAGCGATTCCCTCCATTGCCATCGGTTTGCTAGGAA
TGCCCTTTAACAACCGGTTTGAAGCTTTTATTTATGCTCCCGGTGAAGCCAATGCAGCCCTTGAAGCAGCGGCTGAGTTT
GAATGGTCCGAGTTTCTGCTGATGGCCGGAAATTCCGTCGGTATCGCTTTAATCGGCATTACTCTGGCTTCGCTGATGTA
CCGGACTCGGAAAGTGGATCCGGCTGCGATCTCAAACCGGTTCCCATCCCTCTATGAGTTCTCCCTCAACAAGTGGTTTA
TTGATGACCTCTATGAGCGCACCATCATCAAGGGCTGTCGCCGGATTGCTCGTCAGGTTCTAGAGGTTGACTTCAAAGTC
GTCGATGGCATCGTTAACTTCGCTGGATTCCTCGCAGTATTGACTGGGGAAGGGCTTAAGTACTTGGAAAGTGGCCGTAT
CCAGTTCTACCTGTTCATCATCTTTATGGCAGTCTTGGGGATCTCGATTGCCACGGTCATCTAA

Upstream 100 bases:

>100_bases
TTAATCTTAAGATTTCTCAGTCGAAAAAGGGCCTGACAATATGTAACAATGTAAAATGTTTTACAAACTGTAAAGTTTTT
TATCGAAAACAGCGTCAACG

Downstream 100 bases:

>100_bases
AGTCTTTTCATTTTTTCCTCGTCGTTGCTCAATCCGTTGAATAGAGATAGCGGTTCATGAATCCAGATACATTTCCTTGG
CTGTCAACCCTGATTTTCTT

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MDSLYQFAWLIPVLPLGGAFLVGLGLISYNKQTNALRQINAAFIVSLMGAAMVLSFSILWSQIQGHEPYLMTFEWASAGA
FHLTMGYTIDHLTSLMLVVVTTVAFLVMIYTDGYMAHDPGYVRFYAYLSLFSFSMLGLVVSPNLVQIYIFWELVGMCSYL
LVGFWYDRKPAANACQKAFVTNRVGDFGLLLGILGLYWATNSFEFDIMGERLQSLVSTGELSGVVAAIFAILVFMGPMAK
SAQFPLHVWLPEAMEGPTPISALIHAATMVAAGVFLVARMFPVFSGIPTAMQFIAWTGAITAFLGATIAVTQNDIKKGLA
YSTISQLGYMVMAMGIGAYSAGLFHLMTHAYFKAMLFLGSGSVIHGMEAVVGHNPALAQDMRLMGGLRKYMPYTGITFLI
GCLAICGIPPFAGFWSKDEILSSAFEANPALWLVGWLTAGLTAFYMFRMYFSTFEGDFRGNINALREQIDGKSPALNFGP
GAMDPRELTMVAEQEAHHHDDHHHADEPHESPITMTLPLMVLAIPSIAIGLLGMPFNNRFEAFIYAPGEANAALEAAAEF
EWSEFLLMAGNSVGIALIGITLASLMYRTRKVDPAAISNRFPSLYEFSLNKWFIDDLYERTIIKGCRRIARQVLEVDFKV
VDGIVNFAGFLAVLTGEGLKYLESGRIQFYLFIIFMAVLGISIATVI

Sequences:

>Translated_687_residues
MDSLYQFAWLIPVLPLGGAFLVGLGLISYNKQTNALRQINAAFIVSLMGAAMVLSFSILWSQIQGHEPYLMTFEWASAGA
FHLTMGYTIDHLTSLMLVVVTTVAFLVMIYTDGYMAHDPGYVRFYAYLSLFSFSMLGLVVSPNLVQIYIFWELVGMCSYL
LVGFWYDRKPAANACQKAFVTNRVGDFGLLLGILGLYWATNSFEFDIMGERLQSLVSTGELSGVVAAIFAILVFMGPMAK
SAQFPLHVWLPEAMEGPTPISALIHAATMVAAGVFLVARMFPVFSGIPTAMQFIAWTGAITAFLGATIAVTQNDIKKGLA
YSTISQLGYMVMAMGIGAYSAGLFHLMTHAYFKAMLFLGSGSVIHGMEAVVGHNPALAQDMRLMGGLRKYMPYTGITFLI
GCLAICGIPPFAGFWSKDEILSSAFEANPALWLVGWLTAGLTAFYMFRMYFSTFEGDFRGNINALREQIDGKSPALNFGP
GAMDPRELTMVAEQEAHHHDDHHHADEPHESPITMTLPLMVLAIPSIAIGLLGMPFNNRFEAFIYAPGEANAALEAAAEF
EWSEFLLMAGNSVGIALIGITLASLMYRTRKVDPAAISNRFPSLYEFSLNKWFIDDLYERTIIKGCRRIARQVLEVDFKV
VDGIVNFAGFLAVLTGEGLKYLESGRIQFYLFIIFMAVLGISIATVI
>Mature_687_residues
MDSLYQFAWLIPVLPLGGAFLVGLGLISYNKQTNALRQINAAFIVSLMGAAMVLSFSILWSQIQGHEPYLMTFEWASAGA
FHLTMGYTIDHLTSLMLVVVTTVAFLVMIYTDGYMAHDPGYVRFYAYLSLFSFSMLGLVVSPNLVQIYIFWELVGMCSYL
LVGFWYDRKPAANACQKAFVTNRVGDFGLLLGILGLYWATNSFEFDIMGERLQSLVSTGELSGVVAAIFAILVFMGPMAK
SAQFPLHVWLPEAMEGPTPISALIHAATMVAAGVFLVARMFPVFSGIPTAMQFIAWTGAITAFLGATIAVTQNDIKKGLA
YSTISQLGYMVMAMGIGAYSAGLFHLMTHAYFKAMLFLGSGSVIHGMEAVVGHNPALAQDMRLMGGLRKYMPYTGITFLI
GCLAICGIPPFAGFWSKDEILSSAFEANPALWLVGWLTAGLTAFYMFRMYFSTFEGDFRGNINALREQIDGKSPALNFGP
GAMDPRELTMVAEQEAHHHDDHHHADEPHESPITMTLPLMVLAIPSIAIGLLGMPFNNRFEAFIYAPGEANAALEAAAEF
EWSEFLLMAGNSVGIALIGITLASLMYRTRKVDPAAISNRFPSLYEFSLNKWFIDDLYERTIIKGCRRIARQVLEVDFKV
VDGIVNFAGFLAVLTGEGLKYLESGRIQFYLFIIFMAVLGISIATVI

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=431, Percent_Identity=36.4269141531323, Blast_Score=261, Evalue=1e-69,
Organism=Escherichia coli, GI1788614, Length=667, Percent_Identity=34.4827586206897, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1788829, Length=365, Percent_Identity=31.7808219178082, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1788827, Length=410, Percent_Identity=29.2682926829268, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788831, Length=358, Percent_Identity=29.0502793296089, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788613, Length=406, Percent_Identity=24.8768472906404, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI2367154, Length=187, Percent_Identity=31.0160427807487, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI145693160, Length=324, Percent_Identity=26.2345679012346, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 75478; Mature: 75478

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]