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The map label for this gene is dapF

Identifier: 158335501

GI number: 158335501

Start: 2352479

End: 2353330

Strand: Reverse

Name: dapF

Synonym: AM1_2349

Alternate gene names: 158335501

Gene position: 2353330-2352479 (Counterclockwise)

Preceding gene: 158335508

Following gene: 158335498

Centisome position: 36.18

GC content: 51.76

Gene sequence:

>852_bases
ATGCGGGTTGAGTTTACAAAATATCAAGGATTGGGAAATGATTTTATCCTGATCGATAATCGCCACCAAGCTCAACCCTG
TTTGACGCCAGACCAAGCGGTGCAGATGTGCGATCGCAACTTCGGTATCGGTGGGGATGGGGTCATTTTTGCCTTACCCC
CAGAAGGGGACACGGACTACACCATGCGCATCTATAACTCGGATGGATCTGAACCCGAGATGTGCGGTAATGGTATTCGC
TGTTTAGCGCGATTTCTGGCCCATTTAGAAGGCAAGCCCCTCCAAACAGACATCACCTATCGGATTCATACCTTGGCAGG
CACCATCACCCCTAGCCTCCAAGCCGATGGCCTGGTAAAGGTGGATATGGGGCCTCCCTTTCTGGTTCCCCAAGAAATTC
CGACCACGTTAGGGGAAGGCACCGATCCAGTGGTGAATCAGCCCCTAGAGGTGGCGGGACAGTCCTGGCCAGTGACCTGC
GTGAGTATGGGCAATCCTCACTGCATTACGTTTGTTGACGATTTAGAGGCGATTGATTTTCAAACTTTAGGCCCTCAGTT
TGAGCATCACCCTGTATTTCCCCAACGCATTAATACGGAATTTATTCAGGTGATTCGCCCGGACTATCTCAAAATGTTGG
TGTGGGAGCGGGGTGCAGGTCCTACCCTAGCCTGTGGAACAGGGGCTTGTGCTGTGTTAGTAGCAGGAGTACTCACAGGT
AAAAGCCAATCTCAAGCCACGATTGAACTGCCCGGCGGTCCGTTGCAGATTCGCTGGGCAGGAGAGGGACAGTCCGTGTT
TATGACGGGGCCTGCAGAAAAAGTATTTACAGGGATTTATGAGACGGGCTAA

Upstream 100 bases:

>100_bases
GATGGTTTATCTTGCCATGATACCCATAAAAATCTTGGCATAGACTTCCCTGACTGCCAGAGTAGTATAGAAAGACGTTG
TTTTTGCCTAAGGAATGCTG

Downstream 100 bases:

>100_bases
CACCACGCTTGGACTAGAGCGGGAGCCTCAATCCCATATATTAATCAATCCTGCATCTTAAACGGATGCTAAAGACGCTA
GTGGATCAGGAATCGTGTCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRVEFTKYQGLGNDFILIDNRHQAQPCLTPDQAVQMCDRNFGIGGDGVIFALPPEGDTDYTMRIYNSDGSEPEMCGNGIR
CLARFLAHLEGKPLQTDITYRIHTLAGTITPSLQADGLVKVDMGPPFLVPQEIPTTLGEGTDPVVNQPLEVAGQSWPVTC
VSMGNPHCITFVDDLEAIDFQTLGPQFEHHPVFPQRINTEFIQVIRPDYLKMLVWERGAGPTLACGTGACAVLVAGVLTG
KSQSQATIELPGGPLQIRWAGEGQSVFMTGPAEKVFTGIYETG

Sequences:

>Translated_283_residues
MRVEFTKYQGLGNDFILIDNRHQAQPCLTPDQAVQMCDRNFGIGGDGVIFALPPEGDTDYTMRIYNSDGSEPEMCGNGIR
CLARFLAHLEGKPLQTDITYRIHTLAGTITPSLQADGLVKVDMGPPFLVPQEIPTTLGEGTDPVVNQPLEVAGQSWPVTC
VSMGNPHCITFVDDLEAIDFQTLGPQFEHHPVFPQRINTEFIQVIRPDYLKMLVWERGAGPTLACGTGACAVLVAGVLTG
KSQSQATIELPGGPLQIRWAGEGQSVFMTGPAEKVFTGIYETG
>Mature_283_residues
MRVEFTKYQGLGNDFILIDNRHQAQPCLTPDQAVQMCDRNFGIGGDGVIFALPPEGDTDYTMRIYNSDGSEPEMCGNGIR
CLARFLAHLEGKPLQTDITYRIHTLAGTITPSLQADGLVKVDMGPPFLVPQEIPTTLGEGTDPVVNQPLEVAGQSWPVTC
VSMGNPHCITFVDDLEAIDFQTLGPQFEHHPVFPQRINTEFIQVIRPDYLKMLVWERGAGPTLACGTGACAVLVAGVLTG
KSQSQATIELPGGPLQIRWAGEGQSVFMTGPAEKVFTGIYETG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:
GO:0005737: Diaminopimelate epimerase
GO:0008652: Diaminopimelate epimerase
GO:0008837: Diaminopimelate epimerase
GO:0009085: Diaminopimelate epimerase
GO:0009089: Diaminopimelate epimerase
GO:0016853: Diaminopimelate epimerase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=278, Percent_Identity=39.568345323741, Blast_Score=214, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_ACAM1 (B0C312)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516673.1
- ProteinModelPortal:   B0C312
- SMR:   B0C312
- GeneID:   5681162
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2349
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   AMAR329726:AM1_2349-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30692; Mature: 30692

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVEFTKYQGLGNDFILIDNRHQAQPCLTPDQAVQMCDRNFGIGGDGVIFALPPEGDTDY
CCEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCE
TMRIYNSDGSEPEMCGNGIRCLARFLAHLEGKPLQTDITYRIHTLAGTITPSLQADGLVK
EEEEECCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCEEE
VDMGPPFLVPQEIPTTLGEGTDPVVNQPLEVAGQSWPVTCVSMGNPHCITFVDDLEAIDF
EECCCCEECCCCCCHHHCCCCCHHHCCCHHHCCCCCCEEEEECCCCEEEEEECCCCCCCH
QTLGPQFEHHPVFPQRINTEFIQVIRPDYLKMLVWERGAGPTLACGTGACAVLVAGVLTG
HHCCCCCCCCCCCHHHCCHHHHHHHCHHHHEEEEEECCCCCEEEECCCHHHHHHHHHHCC
KSQSQATIELPGGPLQIRWAGEGQSVFMTGPAEKVFTGIYETG
CCCCCEEEECCCCCEEEEECCCCCEEEEECCHHHHHHHHHCCC
>Mature Secondary Structure
MRVEFTKYQGLGNDFILIDNRHQAQPCLTPDQAVQMCDRNFGIGGDGVIFALPPEGDTDY
CCEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCE
TMRIYNSDGSEPEMCGNGIRCLARFLAHLEGKPLQTDITYRIHTLAGTITPSLQADGLVK
EEEEECCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCEEE
VDMGPPFLVPQEIPTTLGEGTDPVVNQPLEVAGQSWPVTCVSMGNPHCITFVDDLEAIDF
EECCCCEECCCCCCHHHCCCCCHHHCCCHHHCCCCCCEEEEECCCCEEEEEECCCCCCCH
QTLGPQFEHHPVFPQRINTEFIQVIRPDYLKMLVWERGAGPTLACGTGACAVLVAGVLTG
HHCCCCCCCCCCCHHHCCHHHHHHHCHHHHEEEEEECCCCCEEEECCCHHHHHHHHHHCC
KSQSQATIELPGGPLQIRWAGEGQSVFMTGPAEKVFTGIYETG
CCCCCEEEECCCCCEEEEECCCCCEEEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA