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The map label for this gene is clpP [H]

Identifier: 158335483

GI number: 158335483

Start: 2335071

End: 2335757

Strand: Reverse

Name: clpP [H]

Synonym: AM1_2331

Alternate gene names: 158335483

Gene position: 2335757-2335071 (Counterclockwise)

Preceding gene: 158335486

Following gene: 158335482

Centisome position: 35.91

GC content: 48.03

Gene sequence:

>687_bases
ATGGAGTCCAGGATTCAAGCAGTTCAAGCCCCTTACGGTGGCGGCGGCGATGCCTATTACCGCACCCCGCCCCCTGATTT
ACAGTCTCTATTGCTAAAAGAGCGGATTGTGTATTTGGGGATGCCCCTATTTTCCTCAGATGATATCAAACGCCAGGTTG
GCTTTGATGTCACAGAGTTGATTATTGCTCAACTGTTGTTCTTGCAATTCGACGATCCTGATAAACCTATTTTCTTTTAC
ATCAATTCCACAGGCACCTCTTGGTATGGTGGCGATGCCATTGGCTTTGAAACAGAAGCTTTTGCCATTTGCGACACCAT
GTCATACATCAAACCCCCAGTCCACACGATTTGTATTGGCCAAGCCATGGGAACTGCGGCAATGATCTTATCAGCAGGAA
CCAAAGGATATCGAGCGAGCTTAGGCCATGCAACCATCGTTCTGAACCAGGCCCGCAGCGGTGCCCAAGGGCAAGCCTCT
GATATTCAAATTAGAGCGCAGGAAGTCCTTGCGAATAAAGCCACAATGCTAGACATTCTGCAGAAAAATACCGGACAAAC
CGCCGAGAAGATTTCGAAAGACATGGACCGGATGTTTTATCTGTCTCCAGAAGAAGCCAAAGACTATGGGTTGATTGATC
GCGTGTTGGCTAGCCAAAAAGAATTACCCACGCCAATGCCCGTCTAA

Upstream 100 bases:

>100_bases
GCTTTAGTGACAATCTGGGAGTACGGTATTTCCCCCAATTCAAATGCCAGAAAATTGCTACAGTGTAACAGTATTGTCAC
CTTTAGGTAGTTGTCAGGCA

Downstream 100 bases:

>100_bases
GAAATGAGTCAACCTGAGTAGTAGCAAAAAGAGAGTTATCGACTATGCCTATTGGTGTTCCTAGTGTTCCCTACCGTATT
CCTGGCAGTCCCTATGAACG

Product: ATP-dependent Clp protease-like protein

Products: NA

Alternate protein names: Endopeptidase Clp-like [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MESRIQAVQAPYGGGGDAYYRTPPPDLQSLLLKERIVYLGMPLFSSDDIKRQVGFDVTELIIAQLLFLQFDDPDKPIFFY
INSTGTSWYGGDAIGFETEAFAICDTMSYIKPPVHTICIGQAMGTAAMILSAGTKGYRASLGHATIVLNQARSGAQGQAS
DIQIRAQEVLANKATMLDILQKNTGQTAEKISKDMDRMFYLSPEEAKDYGLIDRVLASQKELPTPMPV

Sequences:

>Translated_228_residues
MESRIQAVQAPYGGGGDAYYRTPPPDLQSLLLKERIVYLGMPLFSSDDIKRQVGFDVTELIIAQLLFLQFDDPDKPIFFY
INSTGTSWYGGDAIGFETEAFAICDTMSYIKPPVHTICIGQAMGTAAMILSAGTKGYRASLGHATIVLNQARSGAQGQAS
DIQIRAQEVLANKATMLDILQKNTGQTAEKISKDMDRMFYLSPEEAKDYGLIDRVLASQKELPTPMPV
>Mature_228_residues
MESRIQAVQAPYGGGGDAYYRTPPPDLQSLLLKERIVYLGMPLFSSDDIKRQVGFDVTELIIAQLLFLQFDDPDKPIFFY
INSTGTSWYGGDAIGFETEAFAICDTMSYIKPPVHTICIGQAMGTAAMILSAGTKGYRASLGHATIVLNQARSGAQGQAS
DIQIRAQEVLANKATMLDILQKNTGQTAEKISKDMDRMFYLSPEEAKDYGLIDRVLASQKELPTPMPV

Specific function: Has lost the two conserved residues (Ser and His) proposed to be part of the active site. Therefore it could be inactive [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=204, Percent_Identity=41.6666666666667, Blast_Score=143, Evalue=1e-34,
Organism=Escherichia coli, GI1786641, Length=195, Percent_Identity=40.5128205128205, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17538017, Length=199, Percent_Identity=38.1909547738693, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI20129427, Length=204, Percent_Identity=41.1764705882353, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: 3.4.21.92

Molecular weight: Translated: 24999; Mature: 24999

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESRIQAVQAPYGGGGDAYYRTPPPDLQSLLLKERIVYLGMPLFSSDDIKRQVGFDVTEL
CCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHH
IIAQLLFLQFDDPDKPIFFYINSTGTSWYGGDAIGFETEAFAICDTMSYIKPPVHTICIG
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHEEEHH
QAMGTAAMILSAGTKGYRASLGHATIVLNQARSGAQGQASDIQIRAQEVLANKATMLDIL
HHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QKNTGQTAEKISKDMDRMFYLSPEEAKDYGLIDRVLASQKELPTPMPV
HCCCCHHHHHHHHHHHHEEEECCHHCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MESRIQAVQAPYGGGGDAYYRTPPPDLQSLLLKERIVYLGMPLFSSDDIKRQVGFDVTEL
CCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHH
IIAQLLFLQFDDPDKPIFFYINSTGTSWYGGDAIGFETEAFAICDTMSYIKPPVHTICIG
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHEEEHH
QAMGTAAMILSAGTKGYRASLGHATIVLNQARSGAQGQASDIQIRAQEVLANKATMLDIL
HHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QKNTGQTAEKISKDMDRMFYLSPEEAKDYGLIDRVLASQKELPTPMPV
HCCCCHHHHHHHHHHHHEEEECCHHCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP imidodiphosphate; dATP degradation, [C]

Metal ions: Ca2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 800 [C]

Specific activity: NA

Km value (mM): 0.21 {ATP}} 1.3 {N-succinyl-Leu-Tyr} [C]

Substrates: NA

Specific reaction: NA

General reaction: Hydrolysis of peptide bonds [C]

Inhibitor: Diisopropyl fluorophosphate ClpAP; Fluorosulfonyl benzoyl adenosine; Kappa-Casein; Mg2+; NEM hydrolysis; Neohydrin; Succinyl -Leu-Tyr4-methyl coumarin7-amide; Xaa-Tyr-Leu-Tyr-Trp [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]