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The map label for this gene is metG [H]

Identifier: 158335466

GI number: 158335466

Start: 2313792

End: 2315390

Strand: Reverse

Name: metG [H]

Synonym: AM1_2314

Alternate gene names: 158335466

Gene position: 2315390-2313792 (Counterclockwise)

Preceding gene: 158335467

Following gene: 158335465

Centisome position: 35.6

GC content: 48.03

Gene sequence:

>1599_bases
ATGACTTCCAGCCAAACCCGACGTTTTGCGCTGACCACGCCGCTCTATTACGTTAACGATTTACCCCATATTGGCAGTGC
CTACACCACGATGGCTGCAGATGCGATCGCAAGATTCCATCGCTTACAAGGGCATGAAGTCTTGCTGATCACGGGGACAG
ATGAGCATGGCCAAAAAATTCAGCGGACGGCTGAACAGCGGGGTAAAGCGCCACAAGACCACTGTGACGAAATCGTTGCT
GGGTACAAAGATTTATGGCAACGCCTCAATATTCAATATGATCGCTTTAGTCGCACCACGAGCCCTCGCCATGGTCAGAT
TGTGGCCGAGTTTTTTCAGCGGGTGTGGGATAAAGGTGACATTTGTCAAGGACATCAGCAGGGCTGGTATTGTGTCGGTT
GCGAAGAATTTAAAGAAGAGCGCGACCTCCTCGAAGACAATCACTGTCCGATCCATAAGCGGCCGGTGGAATGGCGAGAC
GAGCAAAATTATTTTTTCCGGCTGTCGAAGTATCAAGACGATCTACTTCAGTTATTTGCGGATCAGCCTGACTTTATCCA
ACCCACCAGTCGCCGGAATGAAGTCCTCAGTTTTGTCGAACGGGGGTTACAGGACTTTTCCATTTCACGGGTGAACTTGG
ATTGGGGATTTCCCGTACCGGCAGACGATCAGCACACCTTATATGTCTGGTTCGATGCCCTCCTCGGCTATGTCACAGCG
CTGCTCGATGAAGATACAGAACCGACCTTAGATCAGGCCCTCAGTCAGTGGTGGCCCATCAATCTCCACTTAATTGGCAA
AGATATTCTGCGGTTTCATGCAGTGTATTGGCCAGCAATGTTGATGTCGGCAGATCTGCCTTTGCCTGATCGGGTATTTG
GTCATGGTTTCTTGACTAAAGATGGGCAAAAAATGGGGAAAAGCCTGGGGAATACCCTCGATCCCGTTGAATTGGTCGAC
CGCTATGGAACGGATGCGGTTCGATACTACTTTTTAAAGGCCCTCGAGTTCGGTCGGGATGGCGACTTCAGTGAAACTCG
CTTTATTGATGTCGTCAATGCTGATCTGGCCAATAGCCTAGGCAATTTACTGAATCGCACCCTTAAAATGGCCGTCAAAT
ATTGTAATGGCCAGGTTCCCGATATTGATATTCAGAGTATTCCGGAGGATCATCCGTTGGCCCAACTGGGTCGAAAAGCG
GGCGATCAGGTCGCAAAAGCTTACCAAAACTTAAATTTTAGTCAAGCTTGTAATATTTCTTTGGGGCTTGTCAAAGCTGG
AAACCAGTTCATAGACGAGCAAGCACCCTGGTCTCTCTATAAAAAAGGGGAACAAGGCTTAGTCAACGAGATCCTTTACA
CCGTTTTAGAATCGGTTAGACTGTCAGCATATCTGCTCACTCCGGTTATTCCTAACCTCAGTACACAGATATATCAACAG
CTAGGGTTCAAGCTTGATTTGAATGTTAATCATGATGGGGATGAGACAACATTACATTCTATTCATAGCAACTGGGGAGG
GTTGCCCGCTAACCAAATCTTAGGCACACCTCAACCCGTCTTCCAAAAATTAGAGCCTTTACAAGCTGCAGCACCCTAG

Upstream 100 bases:

>100_bases
ATTGTACCTATTAAAAACATCATAATTAGGTATAAACGCCGTTCATTAATAGCTCATGTATGATGGAGGTCGGCAACCAC
AATGCTTCTATCTTTAGCCT

Downstream 100 bases:

>100_bases
CACTTTGCCGTCTTCACTTGAGGATTGTCTCAGGGTTACTGCACTGGTTTAGTCAATATATTTTAAAACCTGACATCATT
TAAACATCCGAGGCTTTAAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTSSQTRRFALTTPLYYVNDLPHIGSAYTTMAADAIARFHRLQGHEVLLITGTDEHGQKIQRTAEQRGKAPQDHCDEIVA
GYKDLWQRLNIQYDRFSRTTSPRHGQIVAEFFQRVWDKGDICQGHQQGWYCVGCEEFKEERDLLEDNHCPIHKRPVEWRD
EQNYFFRLSKYQDDLLQLFADQPDFIQPTSRRNEVLSFVERGLQDFSISRVNLDWGFPVPADDQHTLYVWFDALLGYVTA
LLDEDTEPTLDQALSQWWPINLHLIGKDILRFHAVYWPAMLMSADLPLPDRVFGHGFLTKDGQKMGKSLGNTLDPVELVD
RYGTDAVRYYFLKALEFGRDGDFSETRFIDVVNADLANSLGNLLNRTLKMAVKYCNGQVPDIDIQSIPEDHPLAQLGRKA
GDQVAKAYQNLNFSQACNISLGLVKAGNQFIDEQAPWSLYKKGEQGLVNEILYTVLESVRLSAYLLTPVIPNLSTQIYQQ
LGFKLDLNVNHDGDETTLHSIHSNWGGLPANQILGTPQPVFQKLEPLQAAAP

Sequences:

>Translated_532_residues
MTSSQTRRFALTTPLYYVNDLPHIGSAYTTMAADAIARFHRLQGHEVLLITGTDEHGQKIQRTAEQRGKAPQDHCDEIVA
GYKDLWQRLNIQYDRFSRTTSPRHGQIVAEFFQRVWDKGDICQGHQQGWYCVGCEEFKEERDLLEDNHCPIHKRPVEWRD
EQNYFFRLSKYQDDLLQLFADQPDFIQPTSRRNEVLSFVERGLQDFSISRVNLDWGFPVPADDQHTLYVWFDALLGYVTA
LLDEDTEPTLDQALSQWWPINLHLIGKDILRFHAVYWPAMLMSADLPLPDRVFGHGFLTKDGQKMGKSLGNTLDPVELVD
RYGTDAVRYYFLKALEFGRDGDFSETRFIDVVNADLANSLGNLLNRTLKMAVKYCNGQVPDIDIQSIPEDHPLAQLGRKA
GDQVAKAYQNLNFSQACNISLGLVKAGNQFIDEQAPWSLYKKGEQGLVNEILYTVLESVRLSAYLLTPVIPNLSTQIYQQ
LGFKLDLNVNHDGDETTLHSIHSNWGGLPANQILGTPQPVFQKLEPLQAAAP
>Mature_531_residues
TSSQTRRFALTTPLYYVNDLPHIGSAYTTMAADAIARFHRLQGHEVLLITGTDEHGQKIQRTAEQRGKAPQDHCDEIVAG
YKDLWQRLNIQYDRFSRTTSPRHGQIVAEFFQRVWDKGDICQGHQQGWYCVGCEEFKEERDLLEDNHCPIHKRPVEWRDE
QNYFFRLSKYQDDLLQLFADQPDFIQPTSRRNEVLSFVERGLQDFSISRVNLDWGFPVPADDQHTLYVWFDALLGYVTAL
LDEDTEPTLDQALSQWWPINLHLIGKDILRFHAVYWPAMLMSADLPLPDRVFGHGFLTKDGQKMGKSLGNTLDPVELVDR
YGTDAVRYYFLKALEFGRDGDFSETRFIDVVNADLANSLGNLLNRTLKMAVKYCNGQVPDIDIQSIPEDHPLAQLGRKAG
DQVAKAYQNLNFSQACNISLGLVKAGNQFIDEQAPWSLYKKGEQGLVNEILYTVLESVRLSAYLLTPVIPNLSTQIYQQL
GFKLDLNVNHDGDETTLHSIHSNWGGLPANQILGTPQPVFQKLEPLQAAAP

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:
GO:0000166: Methionyl-tRNA synthetase
GO:0004812: Methionyl-tRNA synthetase
GO:0004825: Methionyl-tRNA synthetase
GO:0005524: Methionyl-tRNA synthetase
GO:0005737: Methionyl-tRNA synthetase
GO:0006412: Methionyl-tRNA synthetase
GO:0006418: Methionyl-tRNA synthetase
GO:0006431: Methionyl-tRNA synthetase
GO:0016874: Methionyl-tRNA synthetase

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=503, Percent_Identity=34.9900596421471, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI14043022, Length=567, Percent_Identity=26.63139329806, Blast_Score=169, Evalue=9e-42,
Organism=Escherichia coli, GI1788432, Length=507, Percent_Identity=26.6272189349112, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI212645096, Length=487, Percent_Identity=30.5954825462012, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17540794, Length=570, Percent_Identity=27.0175438596491, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71992781, Length=382, Percent_Identity=28.7958115183246, Blast_Score=185, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321610, Length=510, Percent_Identity=31.3725490196078, Blast_Score=255, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321704, Length=517, Percent_Identity=23.21083172147, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21358483, Length=506, Percent_Identity=35.1778656126482, Blast_Score=292, Evalue=5e-79,
Organism=Drosophila melanogaster, GI24655311, Length=575, Percent_Identity=26.0869565217391, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 60645; Mature: 60514

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSQTRRFALTTPLYYVNDLPHIGSAYTTMAADAIARFHRLQGHEVLLITGTDEHGQKI
CCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
QRTAEQRGKAPQDHCDEIVAGYKDLWQRLNIQYDRFSRTTSPRHGQIVAEFFQRVWDKGD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCC
ICQGHQQGWYCVGCEEFKEERDLLEDNHCPIHKRPVEWRDEQNYFFRLSKYQDDLLQLFA
CCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHC
DQPDFIQPTSRRNEVLSFVERGLQDFSISRVNLDWGFPVPADDQHTLYVWFDALLGYVTA
CCCCCCCCCHHHHHHHHHHHHCHHHCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHH
LLDEDTEPTLDQALSQWWPINLHLIGKDILRFHAVYWPAMLMSADLPLPDRVFGHGFLTK
HHCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
DGQKMGKSLGNTLDPVELVDRYGTDAVRYYFLKALEFGRDGDFSETRFIDVVNADLANSL
CHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHH
GNLLNRTLKMAVKYCNGQVPDIDIQSIPEDHPLAQLGRKAGDQVAKAYQNLNFSQACNIS
HHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCE
LGLVKAGNQFIDEQAPWSLYKKGEQGLVNEILYTVLESVRLSAYLLTPVIPNLSTQIYQQ
EEEECCCCHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LGFKLDLNVNHDGDETTLHSIHSNWGGLPANQILGTPQPVFQKLEPLQAAAP
CCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSSQTRRFALTTPLYYVNDLPHIGSAYTTMAADAIARFHRLQGHEVLLITGTDEHGQKI
CCCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
QRTAEQRGKAPQDHCDEIVAGYKDLWQRLNIQYDRFSRTTSPRHGQIVAEFFQRVWDKGD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCC
ICQGHQQGWYCVGCEEFKEERDLLEDNHCPIHKRPVEWRDEQNYFFRLSKYQDDLLQLFA
CCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHC
DQPDFIQPTSRRNEVLSFVERGLQDFSISRVNLDWGFPVPADDQHTLYVWFDALLGYVTA
CCCCCCCCCHHHHHHHHHHHHCHHHCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHH
LLDEDTEPTLDQALSQWWPINLHLIGKDILRFHAVYWPAMLMSADLPLPDRVFGHGFLTK
HHCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
DGQKMGKSLGNTLDPVELVDRYGTDAVRYYFLKALEFGRDGDFSETRFIDVVNADLANSL
CHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHH
GNLLNRTLKMAVKYCNGQVPDIDIQSIPEDHPLAQLGRKAGDQVAKAYQNLNFSQACNIS
HHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCE
LGLVKAGNQFIDEQAPWSLYKKGEQGLVNEILYTVLESVRLSAYLLTPVIPNLSTQIYQQ
EEEECCCCHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LGFKLDLNVNHDGDETTLHSIHSNWGGLPANQILGTPQPVFQKLEPLQAAAP
CCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]