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The map label for this gene is kdsB

Identifier: 158335349

GI number: 158335349

Start: 2197695

End: 2198432

Strand: Direct

Name: kdsB

Synonym: AM1_2194

Alternate gene names: 158335349

Gene position: 2197695-2198432 (Clockwise)

Preceding gene: 158335348

Following gene: 158335350

Centisome position: 33.79

GC content: 52.3

Gene sequence:

>738_bases
ATGAATATTCTTGCGGTCATCCCTGCCCGCTATCAATCTCAACGCTTTCCTGGCAAGCCCTTAGTGATGCTAGATGAGCG
GCCCATGGTTCAGTGGGTGTATGAAGCAGCGAAAAGCTGCGATTTTTTTCAAGATGCGGTGGTCGCCACGGATAGCGACA
AAATTGCCGACTGTGTCAAAGGGTTTGGCGGCAAAGTGGTGATGACCCGGGACGATCACCTCACAGGCACCGATCGCGTC
GCCGAGGTCGCGGGTTTCTACGATGACATGGACGTCGTCGTCAATGTTCAGGGAGATCAACCCTTTGTCACCCCGGAAGC
CCTAGAACAGCTCGTCCGACCCTACCGAGAGGGCGAACGCCCCGAGATGACCACCCTGGGTTGTCCTTTAGATATGGATG
AGGATTACGCTAGCCCGAATGCGGTGAAAGTATTATGCGATCGCAACGGCCATGCTCTCTATTTTTCCCGTTCTCCCATT
CCTTACTTTCGCACCCAGGGAACGGTACCGGTCTACCACCACTTGGGACTCTATGCCTTTCGCCATGACTTCCTCATGCA
GTACTCCCAACTGGAGCCGACCCCCTTTGAAACCTGCGAAGGCTTAGAGCAATTGCGGGTTTTAGAATATGGCTATGCCA
TTAAGGTCTGCCAAACCCAAAAAGCGGCGATTGAAATTAATACCCCCGAGGATTTAGTTAAAGCCCAGTTATTCATCCAA
CAAGGAATGACCTCATGA

Upstream 100 bases:

>100_bases
AAGATCGTCCGTCTCAAATTTCGGTCTTGCCCGTCGTCGATCCTGACGATCGTTGCGTGGGAGTATTGAGACTGCACGAT
ATTGCTCAAGCGGGTCTTAT

Downstream 100 bases:

>100_bases
TCCGTACTCAAGTGACTGATACATTAGCCATCGGCGAAGGATGTCCGCTGACACTGCTGAGTGGTCCCTGTGTGATCGAA
TCCGAAGACTTTACTTTAAA

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MNILAVIPARYQSQRFPGKPLVMLDERPMVQWVYEAAKSCDFFQDAVVATDSDKIADCVKGFGGKVVMTRDDHLTGTDRV
AEVAGFYDDMDVVVNVQGDQPFVTPEALEQLVRPYREGERPEMTTLGCPLDMDEDYASPNAVKVLCDRNGHALYFSRSPI
PYFRTQGTVPVYHHLGLYAFRHDFLMQYSQLEPTPFETCEGLEQLRVLEYGYAIKVCQTQKAAIEINTPEDLVKAQLFIQ
QGMTS

Sequences:

>Translated_245_residues
MNILAVIPARYQSQRFPGKPLVMLDERPMVQWVYEAAKSCDFFQDAVVATDSDKIADCVKGFGGKVVMTRDDHLTGTDRV
AEVAGFYDDMDVVVNVQGDQPFVTPEALEQLVRPYREGERPEMTTLGCPLDMDEDYASPNAVKVLCDRNGHALYFSRSPI
PYFRTQGTVPVYHHLGLYAFRHDFLMQYSQLEPTPFETCEGLEQLRVLEYGYAIKVCQTQKAAIEINTPEDLVKAQLFIQ
QGMTS
>Mature_245_residues
MNILAVIPARYQSQRFPGKPLVMLDERPMVQWVYEAAKSCDFFQDAVVATDSDKIADCVKGFGGKVVMTRDDHLTGTDRV
AEVAGFYDDMDVVVNVQGDQPFVTPEALEQLVRPYREGERPEMTTLGCPLDMDEDYASPNAVKVLCDRNGHALYFSRSPI
PYFRTQGTVPVYHHLGLYAFRHDFLMQYSQLEPTPFETCEGLEQLRVLEYGYAIKVCQTQKAAIEINTPEDLVKAQLFIQ
QGMTS

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=250, Percent_Identity=42, Blast_Score=177, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_ACAM1 (B0BZZ7)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516521.1
- ProteinModelPortal:   B0BZZ7
- SMR:   B0BZZ7
- GeneID:   5681008
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2194
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- ProtClustDB:   PRK05450
- BioCyc:   AMAR329726:AM1_2194-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27707; Mature: 27707

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILAVIPARYQSQRFPGKPLVMLDERPMVQWVYEAAKSCDFFQDAVVATDSDKIADCVK
CCEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHEEECCCHHHHHHHH
GFGGKVVMTRDDHLTGTDRVAEVAGFYDDMDVVVNVQGDQPFVTPEALEQLVRPYREGER
CCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCC
PEMTTLGCPLDMDEDYASPNAVKVLCDRNGHALYFSRSPIPYFRTQGTVPVYHHLGLYAF
CCEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCEECCCCCHHHHHHHHHHH
RHDFLMQYSQLEPTPFETCEGLEQLRVLEYGYAIKVCQTQKAAIEINTPEDLVKAQLFIQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCHHHHHHHHHHHH
QGMTS
HCCCC
>Mature Secondary Structure
MNILAVIPARYQSQRFPGKPLVMLDERPMVQWVYEAAKSCDFFQDAVVATDSDKIADCVK
CCEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHEEECCCHHHHHHHH
GFGGKVVMTRDDHLTGTDRVAEVAGFYDDMDVVVNVQGDQPFVTPEALEQLVRPYREGER
CCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCC
PEMTTLGCPLDMDEDYASPNAVKVLCDRNGHALYFSRSPIPYFRTQGTVPVYHHLGLYAF
CCEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCEECCCCCHHHHHHHHHHH
RHDFLMQYSQLEPTPFETCEGLEQLRVLEYGYAIKVCQTQKAAIEINTPEDLVKAQLFIQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCHHHHHHHHHHHH
QGMTS
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA