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The map label for this gene is kdsA

Identifier: 158335350

GI number: 158335350

Start: 2198429

End: 2199265

Strand: Direct

Name: kdsA

Synonym: AM1_2195

Alternate gene names: 158335350

Gene position: 2198429-2199265 (Clockwise)

Preceding gene: 158335349

Following gene: 158335351

Centisome position: 33.8

GC content: 53.76

Gene sequence:

>837_bases
ATGATCCGTACTCAAGTGACTGATACATTAGCCATCGGCGAAGGATGTCCGCTGACACTGCTGAGTGGTCCCTGTGTGAT
CGAATCCGAAGACTTTACTTTAAAAATGGCCTCGGGCATCGCTCGCATCTGTGAGAAGCTGGGCATTTCCTATGTGTTCA
AGTCCTCCTTTGATAAAGCCAACCGCACCTCCATTAGCTCCTTTCGGGGGCATCCCATGGAAGAAGGCTTGAGAATCCTC
CAGCGCGTGAAAGATGAGGTGGGCGTCCCCGTCGTTACGGATATTCATGAAAGTCACCAGGCAGCCCCCGTGGCGGAAGT
GGCCGATATCCTCCAAATTCCTGCGTTTTTATGTCGCCAAACGGATCTACTGATGGCGGCAGCAGCCACAGGGCGGACCA
TTAATGTCAAAAAAGGGCAATTTTTAGCCCCCTGGGACATGAAAAACGTGGTCAGCAAACTGGAGGCGGGCGGCGCCAAG
AAAATCCTGCTAACGGAGCGCGGCACTAGCTTTGGCTATAACGCCCTCGTGGTTGATTTCCGATCTCTGCCCCAAATGCG
CAGCTTTGGGTATCCTGTTGTCTTTGATGCTACCCACAGTGTCCAAATGCCCGGCGGCCAAGGCAGCAGTTCCGGTGGTC
AGCGGGAATATGCCCCCTATCTGGCTCGGGCAGCCGCTGCTATTGGTGTGGATGCCCTATTTATGGAAGTGCATGAGAAT
CCCGACCAGGCCCCCAGCGATGGTCCTAATATGATCCCGCTCCATCAGCTCGAAGATGTGTTGCGGCCCATCCTTGAGGT
GCATAAAATCATGAACTCAACTCCCGTATTAGCCTGA

Upstream 100 bases:

>100_bases
ATGCCATTAAGGTCTGCCAAACCCAAAAAGCGGCGATTGAAATTAATACCCCCGAGGATTTAGTTAAAGCCCAGTTATTC
ATCCAACAAGGAATGACCTC

Downstream 100 bases:

>100_bases
TGAGTATCTCCCCAGACCTACAATCTCGGCTCGCCAATGTTAAGCTTTTGGCCTTAGATGTGGATGGCGTCTTAACGGAC
GGCGGGCTGTACTACACCGA

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MIRTQVTDTLAIGEGCPLTLLSGPCVIESEDFTLKMASGIARICEKLGISYVFKSSFDKANRTSISSFRGHPMEEGLRIL
QRVKDEVGVPVVTDIHESHQAAPVAEVADILQIPAFLCRQTDLLMAAAATGRTINVKKGQFLAPWDMKNVVSKLEAGGAK
KILLTERGTSFGYNALVVDFRSLPQMRSFGYPVVFDATHSVQMPGGQGSSSGGQREYAPYLARAAAAIGVDALFMEVHEN
PDQAPSDGPNMIPLHQLEDVLRPILEVHKIMNSTPVLA

Sequences:

>Translated_278_residues
MIRTQVTDTLAIGEGCPLTLLSGPCVIESEDFTLKMASGIARICEKLGISYVFKSSFDKANRTSISSFRGHPMEEGLRIL
QRVKDEVGVPVVTDIHESHQAAPVAEVADILQIPAFLCRQTDLLMAAAATGRTINVKKGQFLAPWDMKNVVSKLEAGGAK
KILLTERGTSFGYNALVVDFRSLPQMRSFGYPVVFDATHSVQMPGGQGSSSGGQREYAPYLARAAAAIGVDALFMEVHEN
PDQAPSDGPNMIPLHQLEDVLRPILEVHKIMNSTPVLA
>Mature_278_residues
MIRTQVTDTLAIGEGCPLTLLSGPCVIESEDFTLKMASGIARICEKLGISYVFKSSFDKANRTSISSFRGHPMEEGLRIL
QRVKDEVGVPVVTDIHESHQAAPVAEVADILQIPAFLCRQTDLLMAAAATGRTINVKKGQFLAPWDMKNVVSKLEAGGAK
KILLTERGTSFGYNALVVDFRSLPQMRSFGYPVVFDATHSVQMPGGQGSSSGGQREYAPYLARAAAAIGVDALFMEVHEN
PDQAPSDGPNMIPLHQLEDVLRPILEVHKIMNSTPVLA

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:
GO:0003824: 2-dehydro-3-deoxyphosphooctonate aldolase
GO:0005737: 2-dehydro-3-deoxyphosphooctonate aldolase
GO:0008152: 2-dehydro-3-deoxyphosphooctonate aldolase
GO:0008676: 2-dehydro-3-deoxyphosphooctonate aldolase
GO:0009058: 2-dehydro-3-deoxyphosphooctonate aldolase
GO:0009103: 2-dehydro-3-deoxyphosphooctonate aldolase
GO:0016740: 2-dehydro-3-deoxyphosphooctonate aldolase

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=255, Percent_Identity=46.6666666666667, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_ACAM1 (B0BZZ8)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516522.1
- ProteinModelPortal:   B0BZZ8
- SMR:   B0BZZ8
- GeneID:   5681009
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2195
- HOGENOM:   HBG575990
- OMA:   GVFLECH
- ProtClustDB:   PRK05198
- BioCyc:   AMAR329726:AM1_2195-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 30077; Mature: 30077

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTQVTDTLAIGEGCPLTLLSGPCVIESEDFTLKMASGIARICEKLGISYVFKSSFDKA
CCCCCCCCEEECCCCCCEEEECCCEEEECCCCEEHHHHHHHHHHHHHCCHHHHHHCCCCC
NRTSISSFRGHPMEEGLRILQRVKDEVGVPVVTDIHESHQAAPVAEVADILQIPAFLCRQ
CCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCCCCHHHHHHHHHHHHHHHHH
TDLLMAAAATGRTINVKKGQFLAPWDMKNVVSKLEAGGAKKILLTERGTSFGYNALVVDF
HHHHHHHHCCCCEEEECCCCEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHEEEEH
RSLPQMRSFGYPVVFDATHSVQMPGGQGSSSGGQREYAPYLARAAAAIGVDALFMEVHEN
HHCHHHHHCCCCEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PDQAPSDGPNMIPLHQLEDVLRPILEVHKIMNSTPVLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIRTQVTDTLAIGEGCPLTLLSGPCVIESEDFTLKMASGIARICEKLGISYVFKSSFDKA
CCCCCCCCEEECCCCCCEEEECCCEEEECCCCEEHHHHHHHHHHHHHCCHHHHHHCCCCC
NRTSISSFRGHPMEEGLRILQRVKDEVGVPVVTDIHESHQAAPVAEVADILQIPAFLCRQ
CCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCCCCHHHHHHHHHHHHHHHHH
TDLLMAAAATGRTINVKKGQFLAPWDMKNVVSKLEAGGAKKILLTERGTSFGYNALVVDF
HHHHHHHHCCCCEEEECCCCEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHEEEEH
RSLPQMRSFGYPVVFDATHSVQMPGGQGSSSGGQREYAPYLARAAAAIGVDALFMEVHEN
HHCHHHHHCCCCEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PDQAPSDGPNMIPLHQLEDVLRPILEVHKIMNSTPVLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA