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The map label for this gene is rfbF [H]

Identifier: 158335343

GI number: 158335343

Start: 2191969

End: 2192736

Strand: Direct

Name: rfbF [H]

Synonym: AM1_2188

Alternate gene names: 158335343

Gene position: 2191969-2192736 (Clockwise)

Preceding gene: 158335342

Following gene: 158335344

Centisome position: 33.7

GC content: 45.57

Gene sequence:

>768_bases
TTGTGTGGTGGCAAAGGAACGAGATTAAAAGAAGAAACCGAGTTTCGTCCCAAGCCCATGGTCATGGTCGGGGATCGTCC
CATGATCTGGCACATTATGAAAATCTATGCTCACTATGGCTTTACAGATTTCCTACTCTGCCTTGGTTATAAAGGCGATA
TGATTCGTGACTATTTCTTTAACTATGATTGGAACCACAGCAATGTCTTGCTGGAATTAGGGAATAAAAAAATTACTAAG
TTAGACAGCTCCCACGGGGAAGAAGATTGGCGGATCTGGCTCATTGATACTGGCCCTGAAACCATGACTGGGGGGCGTTT
AAAGCGTCTATCTCCTTATTTTAAGGATATCGGTAGTGACCTGTTTATGGGCACCTATGGCGATGGCGTGAGTAACGTCA
ATATCCAGAAATTACTGGAGTTCCACAATAGTCATGGCAAGCTAGCCACCATCACCTCTGTTCGCCCTCCGTCGCGTTTT
GGTGAGCTGGCCATTGATAACAATTTTGTCAGCTACTTCCAAGAGAAGCCTCAAACCAGTGAAGGCTGGATCAACGGCGG
GTACTTTGTGTTTCATCGCAAAGTTCTAGATCTGATTCAAGGGGATAGTACCGTTCTGGAAGCTGAGCCGTTTAAGGCGT
TGGCGGCGTTGGGAGAACTCGCCGTCTATAAACATGATGGATTTTGGCAATGTATGGATACCTATAGAGAACTCGAACTG
CTCAATAAGCTATACATCTCCAATCAAGCAGAGTGGAGGGTTTGGTAA

Upstream 100 bases:

>100_bases
CAGCTTTATCTTTAGTTTTTATTAACTGTTTCAAATCGATTGTTAGCGTTGTTCATTGAGTGAGCTAATGGAGACAAAAC
CAAGAATTCCTGTTGCAATT

Downstream 100 bases:

>100_bases
TGAGTGACTTTTGGCAAGATCGATCGGTATTGATTACGGGCTGCACCGGGCTATTAGGCAGTTGGATGACCCAAGAGCTG
GTAGCCCGGGGGGCTCGTGT

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MCGGKGTRLKEETEFRPKPMVMVGDRPMIWHIMKIYAHYGFTDFLLCLGYKGDMIRDYFFNYDWNHSNVLLELGNKKITK
LDSSHGEEDWRIWLIDTGPETMTGGRLKRLSPYFKDIGSDLFMGTYGDGVSNVNIQKLLEFHNSHGKLATITSVRPPSRF
GELAIDNNFVSYFQEKPQTSEGWINGGYFVFHRKVLDLIQGDSTVLEAEPFKALAALGELAVYKHDGFWQCMDTYRELEL
LNKLYISNQAEWRVW

Sequences:

>Translated_255_residues
MCGGKGTRLKEETEFRPKPMVMVGDRPMIWHIMKIYAHYGFTDFLLCLGYKGDMIRDYFFNYDWNHSNVLLELGNKKITK
LDSSHGEEDWRIWLIDTGPETMTGGRLKRLSPYFKDIGSDLFMGTYGDGVSNVNIQKLLEFHNSHGKLATITSVRPPSRF
GELAIDNNFVSYFQEKPQTSEGWINGGYFVFHRKVLDLIQGDSTVLEAEPFKALAALGELAVYKHDGFWQCMDTYRELEL
LNKLYISNQAEWRVW
>Mature_255_residues
MCGGKGTRLKEETEFRPKPMVMVGDRPMIWHIMKIYAHYGFTDFLLCLGYKGDMIRDYFFNYDWNHSNVLLELGNKKITK
LDSSHGEEDWRIWLIDTGPETMTGGRLKRLSPYFKDIGSDLFMGTYGDGVSNVNIQKLLEFHNSHGKLATITSVRPPSRF
GELAIDNNFVSYFQEKPQTSEGWINGGYFVFHRKVLDLIQGDSTVLEAEPFKALAALGELAVYKHDGFWQCMDTYRELEL
LNKLYISNQAEWRVW

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=234, Percent_Identity=27.3504273504274, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI11761619, Length=234, Percent_Identity=27.3504273504274, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133931050, Length=234, Percent_Identity=31.1965811965812, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320148, Length=259, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21355443, Length=255, Percent_Identity=29.0196078431373, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24644084, Length=255, Percent_Identity=29.0196078431373, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 29534; Mature: 29534

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGGKGTRLKEETEFRPKPMVMVGDRPMIWHIMKIYAHYGFTDFLLCLGYKGDMIRDYFF
CCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHC
NYDWNHSNVLLELGNKKITKLDSSHGEEDWRIWLIDTGPETMTGGRLKRLSPYFKDIGSD
CCCCCCCEEEEEECCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHCCHHHHHCCCC
LFMGTYGDGVSNVNIQKLLEFHNSHGKLATITSVRPPSRFGELAIDNNFVSYFQEKPQTS
EEEECCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHCCEEECCHHHHHHHHCCCCC
EGWINGGYFVFHRKVLDLIQGDSTVLEAEPFKALAALGELAVYKHDGFWQCMDTYRELEL
CCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHH
LNKLYISNQAEWRVW
HHHHHHCCCCCEECC
>Mature Secondary Structure
MCGGKGTRLKEETEFRPKPMVMVGDRPMIWHIMKIYAHYGFTDFLLCLGYKGDMIRDYFF
CCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHC
NYDWNHSNVLLELGNKKITKLDSSHGEEDWRIWLIDTGPETMTGGRLKRLSPYFKDIGSD
CCCCCCCEEEEEECCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHCCHHHHHCCCC
LFMGTYGDGVSNVNIQKLLEFHNSHGKLATITSVRPPSRFGELAIDNNFVSYFQEKPQTS
EEEECCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHCCEEECCHHHHHHHHCCCCC
EGWINGGYFVFHRKVLDLIQGDSTVLEAEPFKALAALGELAVYKHDGFWQCMDTYRELEL
CCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHH
LNKLYISNQAEWRVW
HHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]