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The map label for this gene is yfnG [H]

Identifier: 158335344

GI number: 158335344

Start: 2192736

End: 2193719

Strand: Direct

Name: yfnG [H]

Synonym: AM1_2189

Alternate gene names: 158335344

Gene position: 2192736-2193719 (Clockwise)

Preceding gene: 158335343

Following gene: 158335345

Centisome position: 33.72

GC content: 47.76

Gene sequence:

>984_bases
ATGAGTGACTTTTGGCAAGATCGATCGGTATTGATTACGGGCTGCACCGGGCTATTAGGCAGTTGGATGACCCAAGAGCT
GGTAGCCCGGGGGGCTCGTGTCGTGGGACTCGTCAGGGATTGGGTGCCACAATCCCGCTTGTTTACGGAAGGACTTTCCG
ACAAAATTACAACGGTTTACGGTGGTATTGAAGATTTAGGGGTGTTGGAGCGGGCGATTAATGAATACGAAGTCGATACC
GTATTCCATTTAGCCGCCCAAACTATTGTAGGAGTTGCCAATCGCGAACCCCTGGGCACCTTTGAAGCCAATATCAAAGG
CACCTGGAATGTATTAGAAGCCTGTCGTCGGGTGGGTGGTGTCAGTCGCATCGTCGTCGCTTCTAGCGATAAGGCCTATG
GCGATCAGGAGATCTTGCCCTACGACGAGCAAACGCCCCTTCAGGGCGAACATCCCTATGATGTGTCCAAAAGTTGCGCG
GATTTACTGTGTCGAACCTACTATGTGACCTACAACCTCCCCGTCTGTATTACCCGTTGCGGGAATTTCTATGGCGGTGG
GGACTTAAATTTCAATCGCATTGTCCCTGATACGATTCGATCGACGTTGCGAGACAAACCCATTGTGATTCGCAGCGATG
GAACTTATATTCGCGACTATTTCTATGTCAAGGATGGGGTGCTGGCCTACCTTCACCTAGCGGAACAAATGGATCGACCG
GAGATATTGGGTGAGGCCTTTAACTTTAGTAATGAGCTACAAATCTCGGTCTTAGAATTAGTCCATAAAATCTTAAAGTT
AATGGGTAAACCTCATCTTGAACCCACGATTTTAAACCAAGCTCAAAATGAAATTAAGCATCAATACCTTTCCGCAGAAA
AGGCAAGACGGCTATTAGATTGGAAATCCCCCTATGATCTGGATGCAGCATTATTAGAAACCATTCAGTGGTACACAGCG
TTCCTTGAGTCCACTTCTACTTAG

Upstream 100 bases:

>100_bases
ATAAACATGATGGATTTTGGCAATGTATGGATACCTATAGAGAACTCGAACTGCTCAATAAGCTATACATCTCCAATCAA
GCAGAGTGGAGGGTTTGGTA

Downstream 100 bases:

>100_bases
CAACTTCTACTTAGCAATTGTATTAGGAAGGATATGCAACTTCATCCCACAAAAATACCGGGCTGTTTTGAATTAGAACT
AAACGTTTTCAAGGATCAGC

Product: NAD-dependent epimerase/dehydratase

Products: CDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSDFWQDRSVLITGCTGLLGSWMTQELVARGARVVGLVRDWVPQSRLFTEGLSDKITTVYGGIEDLGVLERAINEYEVDT
VFHLAAQTIVGVANREPLGTFEANIKGTWNVLEACRRVGGVSRIVVASSDKAYGDQEILPYDEQTPLQGEHPYDVSKSCA
DLLCRTYYVTYNLPVCITRCGNFYGGGDLNFNRIVPDTIRSTLRDKPIVIRSDGTYIRDYFYVKDGVLAYLHLAEQMDRP
EILGEAFNFSNELQISVLELVHKILKLMGKPHLEPTILNQAQNEIKHQYLSAEKARRLLDWKSPYDLDAALLETIQWYTA
FLESTST

Sequences:

>Translated_327_residues
MSDFWQDRSVLITGCTGLLGSWMTQELVARGARVVGLVRDWVPQSRLFTEGLSDKITTVYGGIEDLGVLERAINEYEVDT
VFHLAAQTIVGVANREPLGTFEANIKGTWNVLEACRRVGGVSRIVVASSDKAYGDQEILPYDEQTPLQGEHPYDVSKSCA
DLLCRTYYVTYNLPVCITRCGNFYGGGDLNFNRIVPDTIRSTLRDKPIVIRSDGTYIRDYFYVKDGVLAYLHLAEQMDRP
EILGEAFNFSNELQISVLELVHKILKLMGKPHLEPTILNQAQNEIKHQYLSAEKARRLLDWKSPYDLDAALLETIQWYTA
FLESTST
>Mature_326_residues
SDFWQDRSVLITGCTGLLGSWMTQELVARGARVVGLVRDWVPQSRLFTEGLSDKITTVYGGIEDLGVLERAINEYEVDTV
FHLAAQTIVGVANREPLGTFEANIKGTWNVLEACRRVGGVSRIVVASSDKAYGDQEILPYDEQTPLQGEHPYDVSKSCAD
LLCRTYYVTYNLPVCITRCGNFYGGGDLNFNRIVPDTIRSTLRDKPIVIRSDGTYIRDYFYVKDGVLAYLHLAEQMDRPE
ILGEAFNFSNELQISVLELVHKILKLMGKPHLEPTILNQAQNEIKHQYLSAEKARRLLDWKSPYDLDAALLETIQWYTAF
LESTST

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=322, Percent_Identity=25.776397515528, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI42516563, Length=325, Percent_Identity=25.8461538461538, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI48994969, Length=338, Percent_Identity=27.810650887574, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788353, Length=337, Percent_Identity=26.4094955489614, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786974, Length=340, Percent_Identity=26.4705882352941, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788366, Length=181, Percent_Identity=30.3867403314917, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=350, Percent_Identity=26.5714285714286, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17539532, Length=324, Percent_Identity=25.6172839506173, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=332, Percent_Identity=23.7951807228916, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=285, Percent_Identity=26.3157894736842, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=188, Percent_Identity=31.3829787234043, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=188, Percent_Identity=31.3829787234043, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=188, Percent_Identity=31.3829787234043, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=188, Percent_Identity=31.3829787234043, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=188, Percent_Identity=31.3829787234043, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=341, Percent_Identity=26.099706744868, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356223, Length=329, Percent_Identity=24.9240121580547, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19923002, Length=341, Percent_Identity=25.8064516129032, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24158427, Length=181, Percent_Identity=28.7292817679558, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013445
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.45

Molecular weight: Translated: 36914; Mature: 36783

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFWQDRSVLITGCTGLLGSWMTQELVARGARVVGLVRDWVPQSRLFTEGLSDKITTVY
CCCCCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHH
GGIEDLGVLERAINEYEVDTVFHLAAQTIVGVANREPLGTFEANIKGTWNVLEACRRVGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHCC
VSRIVVASSDKAYGDQEILPYDEQTPLQGEHPYDVSKSCADLLCRTYYVTYNLPVCITRC
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCHHHHHHC
GNFYGGGDLNFNRIVPDTIRSTLRDKPIVIRSDGTYIRDYFYVKDGVLAYLHLAEQMDRP
CCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCEEEEEEEHHHHHHHHHHHHHHCCCH
EILGEAFNFSNELQISVLELVHKILKLMGKPHLEPTILNQAQNEIKHQYLSAEKARRLLD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
WKSPYDLDAALLETIQWYTAFLESTST
CCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDFWQDRSVLITGCTGLLGSWMTQELVARGARVVGLVRDWVPQSRLFTEGLSDKITTVY
CCCCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHH
GGIEDLGVLERAINEYEVDTVFHLAAQTIVGVANREPLGTFEANIKGTWNVLEACRRVGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHCC
VSRIVVASSDKAYGDQEILPYDEQTPLQGEHPYDVSKSCADLLCRTYYVTYNLPVCITRC
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCHHHHHHC
GNFYGGGDLNFNRIVPDTIRSTLRDKPIVIRSDGTYIRDYFYVKDGVLAYLHLAEQMDRP
CCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCEEEEEEEHHHHHHHHHHHHHHCCCH
EILGEAFNFSNELQISVLELVHKILKLMGKPHLEPTILNQAQNEIKHQYLSAEKARRLLD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
WKSPYDLDAALLETIQWYTAFLESTST
CCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CDPglucose

Specific reaction: CDP-glucose = CDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]