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The map label for this gene is gidA

Identifier: 158335338

GI number: 158335338

Start: 2185946

End: 2187853

Strand: Direct

Name: gidA

Synonym: AM1_2182

Alternate gene names: 158335338

Gene position: 2185946-2187853 (Clockwise)

Preceding gene: 158335336

Following gene: 158335339

Centisome position: 33.61

GC content: 53.46

Gene sequence:

>1908_bases
ATGACGTCCCCCCAGGCAGTTGAATTTTTGGATGAGTTTGAAGTGATTGTCGTCGGGGCAGGCCATGCCGGGTGTGAAGC
TGCGTTAGCCTCTGCTCGCATGGGCTGCAGTACCCTATTGCTCACCCTCAATCTCGACAAAATTGCTTGGCAACCCTGCA
ATCCTGCGGTGGGAGCCCCCGCCAAATCTCAGCTCACCCATGAAGTCGATGCCCTGGGCGGCGAAATTGGCAAAATGGCG
GATCGCACCTATTTGCAAAAGCGTCTGCTCAACCATTCCCGGGGTCCTGCCGTTTGGGCCTTACGGGCTCAGACCGACAA
ACGCGAATATGCGGCAGTGATGAAAACCATCGTCGAGAACCAGGGCAATCTGACCGTGCGGGAAGGTATGGTCACGGATT
TAGTCCTGGGAGACAATGATGAGGTCGTTGGAGTCCAGACCTATTTTGGCGTCGCCTTTCGCTGCCAGGCCGTTATTCTG
ACCACAGGCACCTTTTTAGGGGGACGCATTTGGGTGGGAGATAAATCCATGCCCGCTGGACGAGCCGGAGAGTTTGCGGC
GGAGGGGCTGACCGAAACCCTCAATCAATTGGGGTTTGAAACGGGACGATTGAAAACTGGGACACCTGCTCGGGTCGACA
AGCGGTCTGCGGACTTTAGTGTGATGGAACCTCAGCCCCCGGATCAGCAGGTGCGTTGGTTTAGTTTTGATCCTGCAGTT
TGGATTGAGCGGGAGCAGATGAACTGTTATATCACCCGCACTACCCCGGAAACCCACCAGTTGATTCAGGACAATTTACA
TCTATCCCCCGTCTATGGCGGCTGGGTCGATGCCAAAGGCCCGCGCTACTGTCCCAGTATTGAAGACAAAATCGTGCGGT
TTGCCGATAAGGCTAGCCATCAGATTTTTATTGAGCCAGAAGGCCGCGATATCCCTGAACTCTATATTCAGGGATTCTCC
ACGGGTCTACCCGAGGCATTGCAGTTACGGATGTTGAGATCGCTGCCCGGTCTCGAACACTGTGCCATGCTCCGCCCAGC
CTACGCCGTGGAATATGATTATCTACCTGCAACCCAGTGTTATCCCACCTTAATGACCAAACGGATCGAGGGTCTCTTCT
GTGCGGGACAAATTAATGGCACCACCGGCTATGAAGAAGCGGCGGCTCAGGGGATTGTTGCCGGTATCAATGCTGCCCGC
AGGGTGAACCAGCAGGAAATGCTCATTTTCCCCCGAGCGGAAAGCTATATTGGTACGCTCATCGACGACCTGTGCACAAA
AGACCTACGAGAGCCCTATCGGATGTTGACCGGCCGATCTGAATATCGATTGCTGTTGCGATCGGACAATGCCGATCAGC
GATTGACCCCCTTGGGGCGAGAAGTCGGTCTGATTGATGATCGACGCTGGCACTTATTTACCCGTAAGCAGGCCACCATC
ACCGCTGAGAAAGAACGACTTCATGCCACCCGGCTAAAAGAGCGAGATCCCGCCGCCCAAGCGATTGTCCAAACAACAGG
TGAAAAAATTAAAGGGTCGATCACCTTATCTGATCTGCTGCGTCGGCCCAAGTTCCATTACTGCGACCTTCAGCAACACG
GCTTAGGCATCGAGACCCTGGAGCGCTCTGAACGGGAAAGCGTTGAAATTGAAATTAAGTATGCAGGGTATATCCAGCGC
CAACAGCGCCAGATTGAGCAGGTTAGTCGGCAAGAACAGCGCAAACTACCTGAAAACATAGATTATGCCAGCATTGAGAC
GTTATCCATGGAAGCCCGGGAGAAATTGGCAAAAGTGCGGCCCCTAACGGTAGGACAGGCATCTCGCATTGGTGGTGTCA
ACCCTTCAGATATCAATGCTTTGCTGTTTTATCTCGAAACATTAGCTCGCTTGTCCCCAGTCAGCTAA

Upstream 100 bases:

>100_bases
TGTCATCCCCCATTTAGGAGCCTTCTAGCCTATCGATCCGAGGAGATTTCAAAGAACCGTTAGAATCAGAGGGAGATCAG
ATTAATTCAGGGCCACATCT

Downstream 100 bases:

>100_bases
AAGCCTGTGATACGATGTTAAAGTCTTGTTGGCTTTGGTCAACGACGGGATGTAGCGCAGTTTGGTAGCGCGCCTGCTTT
GGGAGCAGGATGTCGCAGGT

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MTSPQAVEFLDEFEVIVVGAGHAGCEAALASARMGCSTLLLTLNLDKIAWQPCNPAVGAPAKSQLTHEVDALGGEIGKMA
DRTYLQKRLLNHSRGPAVWALRAQTDKREYAAVMKTIVENQGNLTVREGMVTDLVLGDNDEVVGVQTYFGVAFRCQAVIL
TTGTFLGGRIWVGDKSMPAGRAGEFAAEGLTETLNQLGFETGRLKTGTPARVDKRSADFSVMEPQPPDQQVRWFSFDPAV
WIEREQMNCYITRTTPETHQLIQDNLHLSPVYGGWVDAKGPRYCPSIEDKIVRFADKASHQIFIEPEGRDIPELYIQGFS
TGLPEALQLRMLRSLPGLEHCAMLRPAYAVEYDYLPATQCYPTLMTKRIEGLFCAGQINGTTGYEEAAAQGIVAGINAAR
RVNQQEMLIFPRAESYIGTLIDDLCTKDLREPYRMLTGRSEYRLLLRSDNADQRLTPLGREVGLIDDRRWHLFTRKQATI
TAEKERLHATRLKERDPAAQAIVQTTGEKIKGSITLSDLLRRPKFHYCDLQQHGLGIETLERSERESVEIEIKYAGYIQR
QQRQIEQVSRQEQRKLPENIDYASIETLSMEAREKLAKVRPLTVGQASRIGGVNPSDINALLFYLETLARLSPVS

Sequences:

>Translated_635_residues
MTSPQAVEFLDEFEVIVVGAGHAGCEAALASARMGCSTLLLTLNLDKIAWQPCNPAVGAPAKSQLTHEVDALGGEIGKMA
DRTYLQKRLLNHSRGPAVWALRAQTDKREYAAVMKTIVENQGNLTVREGMVTDLVLGDNDEVVGVQTYFGVAFRCQAVIL
TTGTFLGGRIWVGDKSMPAGRAGEFAAEGLTETLNQLGFETGRLKTGTPARVDKRSADFSVMEPQPPDQQVRWFSFDPAV
WIEREQMNCYITRTTPETHQLIQDNLHLSPVYGGWVDAKGPRYCPSIEDKIVRFADKASHQIFIEPEGRDIPELYIQGFS
TGLPEALQLRMLRSLPGLEHCAMLRPAYAVEYDYLPATQCYPTLMTKRIEGLFCAGQINGTTGYEEAAAQGIVAGINAAR
RVNQQEMLIFPRAESYIGTLIDDLCTKDLREPYRMLTGRSEYRLLLRSDNADQRLTPLGREVGLIDDRRWHLFTRKQATI
TAEKERLHATRLKERDPAAQAIVQTTGEKIKGSITLSDLLRRPKFHYCDLQQHGLGIETLERSERESVEIEIKYAGYIQR
QQRQIEQVSRQEQRKLPENIDYASIETLSMEAREKLAKVRPLTVGQASRIGGVNPSDINALLFYLETLARLSPVS
>Mature_634_residues
TSPQAVEFLDEFEVIVVGAGHAGCEAALASARMGCSTLLLTLNLDKIAWQPCNPAVGAPAKSQLTHEVDALGGEIGKMAD
RTYLQKRLLNHSRGPAVWALRAQTDKREYAAVMKTIVENQGNLTVREGMVTDLVLGDNDEVVGVQTYFGVAFRCQAVILT
TGTFLGGRIWVGDKSMPAGRAGEFAAEGLTETLNQLGFETGRLKTGTPARVDKRSADFSVMEPQPPDQQVRWFSFDPAVW
IEREQMNCYITRTTPETHQLIQDNLHLSPVYGGWVDAKGPRYCPSIEDKIVRFADKASHQIFIEPEGRDIPELYIQGFST
GLPEALQLRMLRSLPGLEHCAMLRPAYAVEYDYLPATQCYPTLMTKRIEGLFCAGQINGTTGYEEAAAQGIVAGINAARR
VNQQEMLIFPRAESYIGTLIDDLCTKDLREPYRMLTGRSEYRLLLRSDNADQRLTPLGREVGLIDDRRWHLFTRKQATIT
AEKERLHATRLKERDPAAQAIVQTTGEKIKGSITLSDLLRRPKFHYCDLQQHGLGIETLERSERESVEIEIKYAGYIQRQ
QRQIEQVSRQEQRKLPENIDYASIETLSMEAREKLAKVRPLTVGQASRIGGVNPSDINALLFYLETLARLSPVS

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:
GO:0002098: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
GO:0005737: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
GO:0008033: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
GO:0050660: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=653, Percent_Identity=41.1944869831547, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI19882217, Length=678, Percent_Identity=39.6755162241888, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI183227703, Length=693, Percent_Identity=38.8167388167388, Blast_Score=421, Evalue=1e-117,
Organism=Escherichia coli, GI2367273, Length=619, Percent_Identity=47.6575121163166, Blast_Score=560, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI17534255, Length=632, Percent_Identity=39.2405063291139, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321202, Length=639, Percent_Identity=42.2535211267606, Blast_Score=462, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24658174, Length=637, Percent_Identity=42.0722135007849, Blast_Score=441, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_ACAM1 (B0BZY6)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516510.1
- ProteinModelPortal:   B0BZY6
- SMR:   B0BZY6
- GeneID:   5680996
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2182
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   AMAR329726:AM1_2182-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70988; Mature: 70857

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPQAVEFLDEFEVIVVGAGHAGCEAALASARMGCSTLLLTLNLDKIAWQPCNPAVGAP
CCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEHHHHCCCCCCCCCCCC
AKSQLTHEVDALGGEIGKMADRTYLQKRLLNHSRGPAVWALRAQTDKREYAAVMKTIVEN
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHC
QGNLTVREGMVTDLVLGDNDEVVGVQTYFGVAFRCQAVILTTGTFLGGRIWVGDKSMPAG
CCCEEEECCCEEEEEECCCCCEEEEHHHHCHHHEEEEEEEEECCEECCEEEECCCCCCCC
RAGEFAAEGLTETLNQLGFETGRLKTGTPARVDKRSADFSVMEPQPPDQQVRWFSFDPAV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEECCCCE
WIEREQMNCYITRTTPETHQLIQDNLHLSPVYGGWVDAKGPRYCPSIEDKIVRFADKASH
EEEEECCEEEEEECCCHHHHHHHCCCEECCCCCCEECCCCCCCCCCHHHHHHHHHHCCCC
QIFIEPEGRDIPELYIQGFSTGLPEALQLRMLRSLPGLEHCAMLRPAYAVEYDYLPATQC
EEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCHHH
YPTLMTKRIEGLFCAGQINGTTGYEEAAAQGIVAGINAARRVNQQEMLIFPRAESYIGTL
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHH
IDDLCTKDLREPYRMLTGRSEYRLLLRSDNADQRLTPLGREVGLIDDRRWHLFTRKQATI
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEE
TAEKERLHATRLKERDPAAQAIVQTTGEKIKGSITLSDLLRRPKFHYCDLQQHGLGIETL
EHHHHHHHHHHHHCCCHHHHHHHHHCCHHHCCCEEHHHHHCCCCCCCCCHHHCCCCHHHH
ERSERESVEIEIKYAGYIQRQQRQIEQVSRQEQRKLPENIDYASIETLSMEAREKLAKVR
HCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
PLTVGQASRIGGVNPSDINALLFYLETLARLSPVS
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSPQAVEFLDEFEVIVVGAGHAGCEAALASARMGCSTLLLTLNLDKIAWQPCNPAVGAP
CCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEHHHHCCCCCCCCCCCC
AKSQLTHEVDALGGEIGKMADRTYLQKRLLNHSRGPAVWALRAQTDKREYAAVMKTIVEN
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHC
QGNLTVREGMVTDLVLGDNDEVVGVQTYFGVAFRCQAVILTTGTFLGGRIWVGDKSMPAG
CCCEEEECCCEEEEEECCCCCEEEEHHHHCHHHEEEEEEEEECCEECCEEEECCCCCCCC
RAGEFAAEGLTETLNQLGFETGRLKTGTPARVDKRSADFSVMEPQPPDQQVRWFSFDPAV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEECCCCE
WIEREQMNCYITRTTPETHQLIQDNLHLSPVYGGWVDAKGPRYCPSIEDKIVRFADKASH
EEEEECCEEEEEECCCHHHHHHHCCCEECCCCCCEECCCCCCCCCCHHHHHHHHHHCCCC
QIFIEPEGRDIPELYIQGFSTGLPEALQLRMLRSLPGLEHCAMLRPAYAVEYDYLPATQC
EEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCHHH
YPTLMTKRIEGLFCAGQINGTTGYEEAAAQGIVAGINAARRVNQQEMLIFPRAESYIGTL
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHH
IDDLCTKDLREPYRMLTGRSEYRLLLRSDNADQRLTPLGREVGLIDDRRWHLFTRKQATI
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEE
TAEKERLHATRLKERDPAAQAIVQTTGEKIKGSITLSDLLRRPKFHYCDLQQHGLGIETL
EHHHHHHHHHHHHCCCHHHHHHHHHCCHHHCCCEEHHHHHCCCCCCCCCHHHCCCCHHHH
ERSERESVEIEIKYAGYIQRQQRQIEQVSRQEQRKLPENIDYASIETLSMEAREKLAKVR
HCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
PLTVGQASRIGGVNPSDINALLFYLETLARLSPVS
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA