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The map label for this gene is ndhI

Identifier: 158335315

GI number: 158335315

Start: 2155743

End: 2156345

Strand: Direct

Name: ndhI

Synonym: AM1_2159

Alternate gene names: 158335315

Gene position: 2155743-2156345 (Clockwise)

Preceding gene: 158335314

Following gene: 158335316

Centisome position: 33.15

GC content: 49.59

Gene sequence:

>603_bases
ATGCTCAAGTTTCTGAATCAAGTCACCAGTTACGGAAAAGAATCTTTCCAAGCGGCTCGTTACATTGGCCAAGGTCTAGC
CGTGACCTTCGACCATATGAAGCGCCGTCCGATTACGGTGCAGTATCCCTATGAAAAGCTGATTCCTTCCGAGCGATTTC
GCGGTCGGATTCACTTTGAATTTGACAAGTGCATTTCTTGCGAAGTCTGTGTTCGAGTATGCCCGATTAATCTGCCCGTG
GTGGATTGGGAGTTCAATAAGGAGATCAAGAAGAAGCAGCTCAAGCACTACAGTATTGACTTTGGGGTCTGTATTTTCTG
TGCCAACTGTGTGGAATACTGTCCCACAAACTGTCTCTCGGTCACCGAAGAGTACGAGCTATCGACCTTCGATCGCCATG
AGCTGAACTTCGATAACGTGGCGATGGGACGTTTACCCTACAAAGTGACGCAAGATCCAATGGTCACCCCTATTCGCGAG
TTTGCTTACTTACCAGAATCAGAAATGGATGGTCATAACGTGGACCATACGGCACCCCGAGCTGGGCAAACCCCCGCCGA
GATTGTCGCCAGCACACCGAAACCCGAAACCAAGGAGGGCTAA

Upstream 100 bases:

>100_bases
TAGCGTTTCCTTTTGCCTTTGGTGGATAGGCACAAGGTAAATTTTCTAAACTTAAAACTAACGCCAGCCTTCATCTGCCG
ACATCTGAGAGTGAACATCC

Downstream 100 bases:

>100_bases
TCAGTGAATCTTGCAGAAGGGGTTCAGCTCGTCTCCCTCATTCTTTTGTCTGTCATGATGCTTGGAGCTGCTTTAGGAGT
GGTTTTATTTGAGAGCATTG

Product: NADH dehydrogenase subunit I

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit I; NDH-1 subunit I; NDH-I

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MLKFLNQVTSYGKESFQAARYIGQGLAVTFDHMKRRPITVQYPYEKLIPSERFRGRIHFEFDKCISCEVCVRVCPINLPV
VDWEFNKEIKKKQLKHYSIDFGVCIFCANCVEYCPTNCLSVTEEYELSTFDRHELNFDNVAMGRLPYKVTQDPMVTPIRE
FAYLPESEMDGHNVDHTAPRAGQTPAEIVASTPKPETKEG

Sequences:

>Translated_200_residues
MLKFLNQVTSYGKESFQAARYIGQGLAVTFDHMKRRPITVQYPYEKLIPSERFRGRIHFEFDKCISCEVCVRVCPINLPV
VDWEFNKEIKKKQLKHYSIDFGVCIFCANCVEYCPTNCLSVTEEYELSTFDRHELNFDNVAMGRLPYKVTQDPMVTPIRE
FAYLPESEMDGHNVDHTAPRAGQTPAEIVASTPKPETKEG
>Mature_200_residues
MLKFLNQVTSYGKESFQAARYIGQGLAVTFDHMKRRPITVQYPYEKLIPSERFRGRIHFEFDKCISCEVCVRVCPINLPV
VDWEFNKEIKKKQLKHYSIDFGVCIFCANCVEYCPTNCLSVTEEYELSTFDRHELNFDNVAMGRLPYKVTQDPMVTPIRE
FAYLPESEMDGHNVDHTAPRAGQTPAEIVASTPKPETKEG

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:
GO:0008137: NAD(P)H-quinone oxidoreductase subunit I
GO:0009055: NAD(P)H-quinone oxidoreductase subunit I
GO:0009579: NAD(P)H-quinone oxidoreductase subunit I
GO:0016020: NAD(P)H-quinone oxidoreductase subunit I
GO:0016491: NAD(P)H-quinone oxidoreductase subunit I
GO:0016651: NAD(P)H-quinone oxidoreductase subunit I
GO:0042651: NAD(P)H-quinone oxidoreductase subunit I
GO:0046872: NAD(P)H-quinone oxidoreductase subunit I
GO:0048038: NAD(P)H-quinone oxidoreductase subunit I
GO:0051536: NAD(P)H-quinone oxidoreductase subunit I
GO:0051539: NAD(P)H-quinone oxidoreductase subunit I
GO:0055114: NAD(P)H-quinone oxidoreductase subunit I

Cell location: Cellular thylakoid membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=120, Percent_Identity=31.6666666666667, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1789075, Length=112, Percent_Identity=31.25, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788617, Length=106, Percent_Identity=29.2452830188679, Blast_Score=60, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=120, Percent_Identity=30.8333333333333, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17864306, Length=120, Percent_Identity=32.5, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDHI_ACAM1 (B0BZW3)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516487.1
- ProteinModelPortal:   B0BZW3
- SMR:   B0BZW3
- GeneID:   5680973
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2159
- HOGENOM:   HBG724359
- OMA:   HYSIDFG
- ProtClustDB:   PRK05888
- BioCyc:   AMAR329726:AM1_2159-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR004497
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR00403
- TIGRFAMs:   TIGR01971

Pfam domain/function: NA

EC number: 1.6.5.3

Molecular weight: Translated: 23057; Mature: 23057

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFLNQVTSYGKESFQAARYIGQGLAVTFDHMKRRPITVQYPYEKLIPSERFRGRIHFE
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCEEEECCHHHCCCCHHHCCEEEEE
FDKCISCEVCVRVCPINLPVVDWEFNKEIKKKQLKHYSIDFGVCIFCANCVEYCPTNCLS
CCCCCCHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHH
VTEEYELSTFDRHELNFDNVAMGRLPYKVTQDPMVTPIREFAYLPESEMDGHNVDHTAPR
HCHHCCCCCCCCCCCCCCCEEECCCCCEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
AGQTPAEIVASTPKPETKEG
CCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLKFLNQVTSYGKESFQAARYIGQGLAVTFDHMKRRPITVQYPYEKLIPSERFRGRIHFE
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCEEEECCHHHCCCCHHHCCEEEEE
FDKCISCEVCVRVCPINLPVVDWEFNKEIKKKQLKHYSIDFGVCIFCANCVEYCPTNCLS
CCCCCCHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHH
VTEEYELSTFDRHELNFDNVAMGRLPYKVTQDPMVTPIREFAYLPESEMDGHNVDHTAPR
HCHHCCCCCCCCCCCCCCCEEECCCCCEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
AGQTPAEIVASTPKPETKEG
CCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA