Click here to switch to the map view.

The map label for this gene is gltA [H]

Identifier: 158335313

GI number: 158335313

Start: 2153261

End: 2154397

Strand: Direct

Name: gltA [H]

Synonym: AM1_2157

Alternate gene names: 158335313

Gene position: 2153261-2154397 (Clockwise)

Preceding gene: 158335312

Following gene: 158335314

Centisome position: 33.11

GC content: 50.04

Gene sequence:

>1137_bases
ATGGTCGCTTGTGAATTTCAACCCGGTTTAGAAGGAATTCCTGCTGCTCAATCCCAGATCAGCTTTGTGAATGGCCAAGT
CGGTAAGTTGGAGTATCGCGGTGTTCCCATTGAAGCCCTCGCAGACAACAGCAGCTTCCTGGAAACGGCCTATCTATTGA
TTTGGGGGGAATTTCCCAAATCGGATGATCTAGACGCTTTTCAAGAAGAAATCACCCATCATCGCCGCATCAAATATCGC
ATCCGAGACATGATGAAGTGTTTTCCCGAAAGCGGTCACCCGATGGATGCCCTACAGGCGTCAGCCGCAGCTCTAGGACT
GTTCTATTCCCGACGGGCTTTAGATGATCCCATTTATATTCGTCGGGCCGTGGTGCGATTACTGGCAAAAATCCCAACGA
TGGTGGCAGCCTTTGAGCATATTCGCAAAGGTAATGACCCGGTGCGACCTCGGGATGATTTGAGCTATGCTGCCAACTTC
CTCTACATGATGAATGAGCAGGAGCCGGACCCCTTTTTAGCCCAAATCTTTGACACCTGCTTGATCCTGCATGCCGAGCA
TACAATGAACGCCTCAACCTTCTCGGCTCGGGTCACCGCCTCTACCCTCACCGATCCCTATGCCATTGTGGCTTCAGCCG
TGGGCACCTTAGCCGGGCCTTTACATGGGGGAGCCAACGAAGAAGTGATCACTATGCTGGCGGAGATCGGCTCGGTGGAG
AATGTCCAGCCTTTCTTAGAAGAGAAGCTCGCCACCAAATCTAAAATTATGGGATTTGGGCATCGTGTCTATAAAGTGAA
GGATCCGAGAGCAAAGATCTTACAGAAATTAGCCGAACAATTGTTTGATAAAACCGGTGGCGATCCCTCCTATGAAATTG
CCCTAGCGCTGGAAAAAGCAGTGGCCGAAAAACTGGGGAGTAAAGGGATTTATCCCAATGTTGATTTTTACTCTGGGCTG
GTTTACCAAAAACTGGGAATTCCCACGGATTTATTTACACCTATCTTTGCGATCGCAAGAGTGGCTGGCTGGTTAGCCCA
CTGGCGAGAACAGTTGGAAACGAACCGGATTTATCGCCCCAGCCAGGTTTATATCGGCGACCATAGTCTTGCTTATATCC
CGCCAGAGAGCCGTTAG

Upstream 100 bases:

>100_bases
AATTGCTGTTTTAGAGCCCATACCCTCCTTCGCCCTGCCCGGCTCCCCCTCAGTGCTTCCAGGCAGTTGGCGATCTATTC
ACCTTCATCTGAGAGACACT

Downstream 100 bases:

>100_bases
AACAGACATAGACTAGAATCAATCCTTTCCTTCAGCCCTTAGGGAAATGGATTTCGGAGCATGAAGTTATTCTCCTAGAA
ACTCGCCTAGGCGTATATAC

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MVACEFQPGLEGIPAAQSQISFVNGQVGKLEYRGVPIEALADNSSFLETAYLLIWGEFPKSDDLDAFQEEITHHRRIKYR
IRDMMKCFPESGHPMDALQASAAALGLFYSRRALDDPIYIRRAVVRLLAKIPTMVAAFEHIRKGNDPVRPRDDLSYAANF
LYMMNEQEPDPFLAQIFDTCLILHAEHTMNASTFSARVTASTLTDPYAIVASAVGTLAGPLHGGANEEVITMLAEIGSVE
NVQPFLEEKLATKSKIMGFGHRVYKVKDPRAKILQKLAEQLFDKTGGDPSYEIALALEKAVAEKLGSKGIYPNVDFYSGL
VYQKLGIPTDLFTPIFAIARVAGWLAHWREQLETNRIYRPSQVYIGDHSLAYIPPESR

Sequences:

>Translated_378_residues
MVACEFQPGLEGIPAAQSQISFVNGQVGKLEYRGVPIEALADNSSFLETAYLLIWGEFPKSDDLDAFQEEITHHRRIKYR
IRDMMKCFPESGHPMDALQASAAALGLFYSRRALDDPIYIRRAVVRLLAKIPTMVAAFEHIRKGNDPVRPRDDLSYAANF
LYMMNEQEPDPFLAQIFDTCLILHAEHTMNASTFSARVTASTLTDPYAIVASAVGTLAGPLHGGANEEVITMLAEIGSVE
NVQPFLEEKLATKSKIMGFGHRVYKVKDPRAKILQKLAEQLFDKTGGDPSYEIALALEKAVAEKLGSKGIYPNVDFYSGL
VYQKLGIPTDLFTPIFAIARVAGWLAHWREQLETNRIYRPSQVYIGDHSLAYIPPESR
>Mature_378_residues
MVACEFQPGLEGIPAAQSQISFVNGQVGKLEYRGVPIEALADNSSFLETAYLLIWGEFPKSDDLDAFQEEITHHRRIKYR
IRDMMKCFPESGHPMDALQASAAALGLFYSRRALDDPIYIRRAVVRLLAKIPTMVAAFEHIRKGNDPVRPRDDLSYAANF
LYMMNEQEPDPFLAQIFDTCLILHAEHTMNASTFSARVTASTLTDPYAIVASAVGTLAGPLHGGANEEVITMLAEIGSVE
NVQPFLEEKLATKSKIMGFGHRVYKVKDPRAKILQKLAEQLFDKTGGDPSYEIALALEKAVAEKLGSKGIYPNVDFYSGL
VYQKLGIPTDLFTPIFAIARVAGWLAHWREQLETNRIYRPSQVYIGDHSLAYIPPESR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:
GO:0005737: 2-methylcitrate synthase/citrate synthase
GO:0006099: 2-methylcitrate synthase/citrate synthase
GO:0016740: 2-methylcitrate synthase/citrate synthase
GO:0044262: 2-methylcitrate synthase/citrate synthase
GO:0046912: 2-methylcitrate synthase/citrate synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=329, Percent_Identity=27.6595744680851, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1786939, Length=373, Percent_Identity=39.4101876675603, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1786527, Length=370, Percent_Identity=33.5135135135135, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17555174, Length=322, Percent_Identity=27.9503105590062, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6319850, Length=386, Percent_Identity=25.9067357512953, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=320, Percent_Identity=24.6875, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6325257, Length=377, Percent_Identity=24.6684350132626, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640126, Length=373, Percent_Identity=25.7372654155496, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24640124, Length=373, Percent_Identity=25.7372654155496, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21356863, Length=379, Percent_Identity=25.3298153034301, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 42143; Mature: 42143

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVACEFQPGLEGIPAAQSQISFVNGQVGKLEYRGVPIEALADNSSFLETAYLLIWGEFPK
CEEECCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHCCCCHHHHHEEEEEEECCCC
SDDLDAFQEEITHHRRIKYRIRDMMKCFPESGHPMDALQASAAALGLFYSRRALDDPIYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
RRAVVRLLAKIPTMVAAFEHIRKGNDPVRPRDDLSYAANFLYMMNEQEPDPFLAQIFDTC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHH
LILHAEHTMNASTFSARVTASTLTDPYAIVASAVGTLAGPLHGGANEEVITMLAEIGSVE
HHHHHHHHCCCCHHHHEEEHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
NVQPFLEEKLATKSKIMGFGHRVYKVKDPRAKILQKLAEQLFDKTGGDPSYEIALALEKA
CHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VAEKLGSKGIYPNVDFYSGLVYQKLGIPTDLFTPIFAIARVAGWLAHWREQLETNRIYRP
HHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SQVYIGDHSLAYIPPESR
CEEEECCCEEEECCCCCC
>Mature Secondary Structure
MVACEFQPGLEGIPAAQSQISFVNGQVGKLEYRGVPIEALADNSSFLETAYLLIWGEFPK
CEEECCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHCCCCHHHHHEEEEEEECCCC
SDDLDAFQEEITHHRRIKYRIRDMMKCFPESGHPMDALQASAAALGLFYSRRALDDPIYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
RRAVVRLLAKIPTMVAAFEHIRKGNDPVRPRDDLSYAANFLYMMNEQEPDPFLAQIFDTC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHH
LILHAEHTMNASTFSARVTASTLTDPYAIVASAVGTLAGPLHGGANEEVITMLAEIGSVE
HHHHHHHHCCCCHHHHEEEHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
NVQPFLEEKLATKSKIMGFGHRVYKVKDPRAKILQKLAEQLFDKTGGDPSYEIALALEKA
CHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VAEKLGSKGIYPNVDFYSGLVYQKLGIPTDLFTPIFAIARVAGWLAHWREQLETNRIYRP
HHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SQVYIGDHSLAYIPPESR
CEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]