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The map label for this gene is sppA [H]

Identifier: 158335227

GI number: 158335227

Start: 2063196

End: 2064143

Strand: Direct

Name: sppA [H]

Synonym: AM1_2070

Alternate gene names: 158335227

Gene position: 2063196-2064143 (Clockwise)

Preceding gene: 158335226

Following gene: 158335228

Centisome position: 31.72

GC content: 49.47

Gene sequence:

>948_bases
ATGCGTCTGAATCGTGTCCTAGCCTTAGCGTTAGTGACGTTATGTTTGATTGCAGCTTTAGGTAACTGGTTAGGTGGATC
GGTTAACAAAAGCAAAGACCAGGGCTCTGCGAATGTCGCCCAAATCAATGTTTATGGCGTGATTAGCGATGAAGCGTCTG
GTGGGCCTTTCAGCTCGGGTGAAGGGGCCAATGCCAATAAGCTGATCAGCAATATCAAAAAAGCTAGAGAGGATGATGGA
ATTAAAGCCATCCTGCTACGGATTAACAGTCCGGGAGGCACAGCCGCCGCATCCCAGGCCGTGTACGAAGAGCTAATGCG
CACCCGCAAGGATACGGATATTAAAATTGTCGCCAGCTTGGGGGATGTCGCTGCTTCAGGAGGATACTACATTGCCAGTG
CCGCGGATCATATTGTGGCGAATCCTTCTTCGATCACCGGATCCATTGGGGTGATTGTGCAAACCCAAAATGTGACCTCT
CTCCTTGATAAAGTGGGGGTCCAAACCAACACCTTTACCAGTGGCCCCTTAAAAGATATTCTGTCGCCGTTCCGAGAGAC
GAAACCCGAAGAACGAAAAATTCTGCAGTCCATCGTGGATGATTCTTATCAGCAGTTTTTAGATGCCATTGTTGCCGGAC
GGGGGATGCCCTTGGAAAAACTGAAGCCCTTAGCCGATGGTCGTATTTATACGGGGACTCAAGCCAAAGACAACAAGCTG
GTAGATTCCCTGGGGAATTACTATGACGCGTTGGACAAAACGGCGGAGCTAGCCAATATTTCCGGTGACCCCAAGGTCAA
GGATTTTGGCAAAGGCAGCTTATTTGGAGATTTCGGTCCATTTCTCTCCACGTCGAATCAATCCTTTCCCCAGCTAACGA
CGGAATGGCTCTCTTCCAAATCCCAATGGCCGCGTTGGCATAAGATCCCGCTAACGATCATGGAGTAA

Upstream 100 bases:

>100_bases
AGCTTATAAAAACAGTCGAACCCTGGTGATAGGGTCAGGTGTCTAATCATCGAGCAGTTTCGTTCCTCACCCATGTTCAG
TAAAGGGAGAATAATCTTCT

Downstream 100 bases:

>100_bases
GCGATGCTAGATGCTCTATTTACGACGTTCTTTGGTGCTCAATCCTCCCGGAATAACCAATCTGTCTCGATTCTGATTGG
CTTTGCGCTTCTCAGCATTC

Product: signal peptide peptidase SppA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRLNRVLALALVTLCLIAALGNWLGGSVNKSKDQGSANVAQINVYGVISDEASGGPFSSGEGANANKLISNIKKAREDDG
IKAILLRINSPGGTAAASQAVYEELMRTRKDTDIKIVASLGDVAASGGYYIASAADHIVANPSSITGSIGVIVQTQNVTS
LLDKVGVQTNTFTSGPLKDILSPFRETKPEERKILQSIVDDSYQQFLDAIVAGRGMPLEKLKPLADGRIYTGTQAKDNKL
VDSLGNYYDALDKTAELANISGDPKVKDFGKGSLFGDFGPFLSTSNQSFPQLTTEWLSSKSQWPRWHKIPLTIME

Sequences:

>Translated_315_residues
MRLNRVLALALVTLCLIAALGNWLGGSVNKSKDQGSANVAQINVYGVISDEASGGPFSSGEGANANKLISNIKKAREDDG
IKAILLRINSPGGTAAASQAVYEELMRTRKDTDIKIVASLGDVAASGGYYIASAADHIVANPSSITGSIGVIVQTQNVTS
LLDKVGVQTNTFTSGPLKDILSPFRETKPEERKILQSIVDDSYQQFLDAIVAGRGMPLEKLKPLADGRIYTGTQAKDNKL
VDSLGNYYDALDKTAELANISGDPKVKDFGKGSLFGDFGPFLSTSNQSFPQLTTEWLSSKSQWPRWHKIPLTIME
>Mature_315_residues
MRLNRVLALALVTLCLIAALGNWLGGSVNKSKDQGSANVAQINVYGVISDEASGGPFSSGEGANANKLISNIKKAREDDG
IKAILLRINSPGGTAAASQAVYEELMRTRKDTDIKIVASLGDVAASGGYYIASAADHIVANPSSITGSIGVIVQTQNVTS
LLDKVGVQTNTFTSGPLKDILSPFRETKPEERKILQSIVDDSYQQFLDAIVAGRGMPLEKLKPLADGRIYTGTQAKDNKL
VDSLGNYYDALDKTAELANISGDPKVKDFGKGSLFGDFGPFLSTSNQSFPQLTTEWLSSKSQWPRWHKIPLTIME

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=191, Percent_Identity=41.3612565445026, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1787527, Length=161, Percent_Identity=32.9192546583851, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 33696; Mature: 33696

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNRVLALALVTLCLIAALGNWLGGSVNKSKDQGSANVAQINVYGVISDEASGGPFSSG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCC
EGANANKLISNIKKAREDDGIKAILLRINSPGGTAAASQAVYEELMRTRKDTDIKIVASL
CCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEH
GDVAASGGYYIASAADHIVANPSSITGSIGVIVQTQNVTSLLDKVGVQTNTFTSGPLKDI
HHHHCCCCEEEECCCCHHCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHH
LSPFRETKPEERKILQSIVDDSYQQFLDAIVAGRGMPLEKLKPLADGRIYTGTQAKDNKL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEECCCCCHHHH
VDSLGNYYDALDKTAELANISGDPKVKDFGKGSLFGDFGPFLSTSNQSFPQLTTEWLSSK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCC
SQWPRWHKIPLTIME
CCCCCEECCCEEECC
>Mature Secondary Structure
MRLNRVLALALVTLCLIAALGNWLGGSVNKSKDQGSANVAQINVYGVISDEASGGPFSSG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCC
EGANANKLISNIKKAREDDGIKAILLRINSPGGTAAASQAVYEELMRTRKDTDIKIVASL
CCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEH
GDVAASGGYYIASAADHIVANPSSITGSIGVIVQTQNVTSLLDKVGVQTNTFTSGPLKDI
HHHHCCCCEEEECCCCHHCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHH
LSPFRETKPEERKILQSIVDDSYQQFLDAIVAGRGMPLEKLKPLADGRIYTGTQAKDNKL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEECCCCCHHHH
VDSLGNYYDALDKTAELANISGDPKVKDFGKGSLFGDFGPFLSTSNQSFPQLTTEWLSSK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCC
SQWPRWHKIPLTIME
CCCCCEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]