Click here to switch to the map view.

The map label for this gene is folK [H]

Identifier: 158335128

GI number: 158335128

Start: 1966364

End: 1966843

Strand: Direct

Name: folK [H]

Synonym: AM1_1969

Alternate gene names: 158335128

Gene position: 1966364-1966843 (Clockwise)

Preceding gene: 158335125

Following gene: 158335130

Centisome position: 30.23

GC content: 54.37

Gene sequence:

>480_bases
GTGCAATCGGCGGTTGCCTTAGGGGGAAATTTAGGCAATCCCAAAGCGACCCTTTCTGCTGCCATTACCACCCTGCAAAA
CGACGCCAAAATTCTGGTGTTGACCCAATCCCATTGGTATCAAACCGCAGCCGTTGGACCACCCCAGCCCGACTATCTCA
ATGGTTGTGTCAGTTTATTGACAGCTTACTCGCCTCAATCCTTGCTAGAGAAGCTATTGGAGATTGAAACTCAGTTTGGA
CGTGTCCGACGGGAGCGCTGGGGACCTCGCACCCTCGATCTAGATGTGTTGTTGTTTGGACAGGCCGTCATTCAGACACC
GACGTTGACGGTGCCCCATCCCTGTCTGCATGAACGCGCTTTTGTGCTGGCCCCTTTGGCCGAGATCTGTCCAGGCTGGG
TGCATCCCGTGCAACAGCAAACCATTGCCGAGCTGGCCAAGGCGGTAGACTATACAGGGGTTCATCGACTGTCTCACTAG

Upstream 100 bases:

>100_bases
TGTATATTCTGTAAATCACTGTTCTCACTGACCGATCGACCTCAAGATCGACCCACCTATGGTTGAATGCTCGGAGATGG
GCGGAGAAAAATCAAATTGG

Downstream 100 bases:

>100_bases
GCAGCGTCATTCAGCTGAGAAGGCATACTCGTTGGTTACCTCTGCCATTTCTTTCACGCATTGAGAGCATGAATTGCAAT
CTCTTTCGTTATTTTCCAAT

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MQSAVALGGNLGNPKATLSAAITTLQNDAKILVLTQSHWYQTAAVGPPQPDYLNGCVSLLTAYSPQSLLEKLLEIETQFG
RVRRERWGPRTLDLDVLLFGQAVIQTPTLTVPHPCLHERAFVLAPLAEICPGWVHPVQQQTIAELAKAVDYTGVHRLSH

Sequences:

>Translated_159_residues
MQSAVALGGNLGNPKATLSAAITTLQNDAKILVLTQSHWYQTAAVGPPQPDYLNGCVSLLTAYSPQSLLEKLLEIETQFG
RVRRERWGPRTLDLDVLLFGQAVIQTPTLTVPHPCLHERAFVLAPLAEICPGWVHPVQQQTIAELAKAVDYTGVHRLSH
>Mature_159_residues
MQSAVALGGNLGNPKATLSAAITTLQNDAKILVLTQSHWYQTAAVGPPQPDYLNGCVSLLTAYSPQSLLEKLLEIETQFG
RVRRERWGPRTLDLDVLLFGQAVIQTPTLTVPHPCLHERAFVLAPLAEICPGWVHPVQQQTIAELAKAVDYTGVHRLSH

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:
GO:0003848: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
GO:0009396: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
GO:0016301: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
GO:0016310: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=134, Percent_Identity=49.2537313432836, Blast_Score=123, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6324073, Length=151, Percent_Identity=31.7880794701987, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17381; Mature: 17381

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSAVALGGNLGNPKATLSAAITTLQNDAKILVLTQSHWYQTAAVGPPQPDYLNGCVSLL
CCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHH
TAYSPQSLLEKLLEIETQFGRVRRERWGPRTLDLDVLLFGQAVIQTPTLTVPHPCLHERA
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
FVLAPLAEICPGWVHPVQQQTIAELAKAVDYTGVHRLSH
HHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSAVALGGNLGNPKATLSAAITTLQNDAKILVLTQSHWYQTAAVGPPQPDYLNGCVSLL
CCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHH
TAYSPQSLLEKLLEIETQFGRVRRERWGPRTLDLDVLLFGQAVIQTPTLTVPHPCLHERA
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
FVLAPLAEICPGWVHPVQQQTIAELAKAVDYTGVHRLSH
HHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]