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The map label for this gene is asnB [H]

Identifier: 158335075

GI number: 158335075

Start: 1912080

End: 1914050

Strand: Reverse

Name: asnB [H]

Synonym: AM1_1915

Alternate gene names: 158335075

Gene position: 1914050-1912080 (Counterclockwise)

Preceding gene: 158335076

Following gene: 158335074

Centisome position: 29.43

GC content: 47.69

Gene sequence:

>1971_bases
ATGAGCGCAATTACTGGCTTTGTCTATCCTCACCCTCATCCTGAGGGGAGCGATTATTTGCACCAAATGGTCCAATCTCT
ATCCCATCGAGGACCGGATGGAACCAATGAATGGAGTCAAGATAGGGTTGGCTTAGGCCATTGTTTATTACAGACCACCC
CAGAATCCCGGTTTGAAACGCTACCCCTTAGCAACGCTTCCCAAACTTTAATCTTGACGGCAGACGCCCGAATTGATAAC
CGTGTGGACCTAATGACCCAGCTGGGTCTGAAAGAGTCTCCCTCAGCAGTGATTACCGATAGCGCCCTCATTCTTGCTGC
CTACGAAAAATGGGGCGAACAATGCCCCGAATATTTCTTAGGTGATTTTGCCTTTGCCCTTTGGGATGAGACTCAACAGC
ATTTGTTTTGCGCCCGCGATCATTTTGGAGTCAAGCCCTTCTTTTACTATGCCTCCGAACAAGTCTTTGCCTTTGCCAGC
GAAATGAAAGCGCTGTTTCATTTGCTGGAAGTGCCTAAATCCCTCAATGAAACCAGGGTCGCCGACTATCTGACCCTGAT
GCTATTTGATGCAGAAATGACTTTTTATGAGCAGATTGTTCGTCTTCCTCCTGCTCATCGGATGACAGTAAGCCAAAACA
GTTTGGACATCTCAGCCTATTGGTCTCTCGATCCTGACTTTGAACTTCAGCTGGACTCCGATCAAGCCTATGCGGAACAG
TTTCAATCCATTTTCACCGAAGCCGTGCGCTGCCGCCTGAGAAGTGCATTTTCCATCGGCACAATGTTAAGTGGAGGAAT
TGATTCTTCCTCGATTACCTGTATGACGGCCCAGTTGAGACAGGAGGCGCAAGAGACCGCTAAATTCCCTACTTTCTCCG
CCGTGTTTAATGAAATTACGGCATCTGATGAGCAGGAATTTATGAATGCCGTGATTCATCAGCGACCCATCGAGCCTCAG
TATGTACAGGGCGATCAACTCAGCCCCTGGACCGATTATCAGCACATGCTAGCTATCCAAGACGAACCCTTATTTGCCTG
TAACGCCCATTTAACCCATGCCTTATATCAAATCGCTCAAAGCCAATCGGTCAGGGTGTTATTGGATGGCTTTGATGGTG
ATCAGGCGGTATCTCATGGTGTTGGGTATTTACGTACTCTGGCCAAGGCAGGACGGTGGCTCAAGTTATGGCGCGAGATG
AGGGGGTTTTGCCAAAGCTTTGGTTACGATTTATGGCCCTTGTATCGGTCCTATGTCCGTAAATTTGGCATCAATCCCCT
CCTTAAAAAAACAAAGATTACCAAAATTTTAGGTAAAGTTAAAAGCTGGCAATTCCGTGGGCAGCAGAAGACGTCCGAGG
TGACTCCAGCCAGGCCCTGGGCCATCCCCCTCACCCCAGACTTTGTTGATCGAGCATCCGTGCTACAGCGTTATAAACAC
CTGCGTCGTCAGCGCTTTGGAGCACAGCCTTCCCAGCGCCACGAACATATCGTTGACGTTACATCTGGCTTAGTCTCTTC
GACCCTAGAAGTGATGGATCGAACAGCTGCTGCCTCCCAAGTGGAATTGAGATTTCCCTTTTGGGATAAGCGGTTAGTGG
AATTTTGTGTTTCTCTACCAGAAGAACAAAAGATGCAAAATGGTTGGACTCGATTAATTATCCGACGTGGACTGGATGGT
ATTCTTCCTCCTCAAATTCAATGGCGTGCCGGTAAAGGAAATTTGGGCACAGCGTTTGACCATACCCTAGAAAAGTACGG
TATGGACCTAATTCATCAAGTTCTCAATCATCCACCCGATGCCATTGAGACGTTTATTGATATGCACGAGTTCACTCAGT
TAGTCCAGCGATGGCAAAGGGGAGAGGCTGAAGCTAACGATACACTGTTGATTTGGATTGCCGTTAATTTGACTCTTTGG
CTACAACAAGCCAATCTAGGCGTCAATTTTGCCGAATCTACTCTACAGTAA

Upstream 100 bases:

>100_bases
GACGATTGTCCTTAGCTCAAAACTTGATTCAAGAGGGATTTCTGATGATTAAAGCGATTGAACCCACTTCTACTTCCTAA
CATTTGCCCCTTCTGCCCCC

Downstream 100 bases:

>100_bases
TCAAATCTAATGAATATTTAAGGATTGGAATGTCCCCAAAATATTTGAGAAGTTATAATAGCTTAACTGGATTTGTATGC
TGAATTGATGTCCGCTTGGC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MSAITGFVYPHPHPEGSDYLHQMVQSLSHRGPDGTNEWSQDRVGLGHCLLQTTPESRFETLPLSNASQTLILTADARIDN
RVDLMTQLGLKESPSAVITDSALILAAYEKWGEQCPEYFLGDFAFALWDETQQHLFCARDHFGVKPFFYYASEQVFAFAS
EMKALFHLLEVPKSLNETRVADYLTLMLFDAEMTFYEQIVRLPPAHRMTVSQNSLDISAYWSLDPDFELQLDSDQAYAEQ
FQSIFTEAVRCRLRSAFSIGTMLSGGIDSSSITCMTAQLRQEAQETAKFPTFSAVFNEITASDEQEFMNAVIHQRPIEPQ
YVQGDQLSPWTDYQHMLAIQDEPLFACNAHLTHALYQIAQSQSVRVLLDGFDGDQAVSHGVGYLRTLAKAGRWLKLWREM
RGFCQSFGYDLWPLYRSYVRKFGINPLLKKTKITKILGKVKSWQFRGQQKTSEVTPARPWAIPLTPDFVDRASVLQRYKH
LRRQRFGAQPSQRHEHIVDVTSGLVSSTLEVMDRTAAASQVELRFPFWDKRLVEFCVSLPEEQKMQNGWTRLIIRRGLDG
ILPPQIQWRAGKGNLGTAFDHTLEKYGMDLIHQVLNHPPDAIETFIDMHEFTQLVQRWQRGEAEANDTLLIWIAVNLTLW
LQQANLGVNFAESTLQ

Sequences:

>Translated_656_residues
MSAITGFVYPHPHPEGSDYLHQMVQSLSHRGPDGTNEWSQDRVGLGHCLLQTTPESRFETLPLSNASQTLILTADARIDN
RVDLMTQLGLKESPSAVITDSALILAAYEKWGEQCPEYFLGDFAFALWDETQQHLFCARDHFGVKPFFYYASEQVFAFAS
EMKALFHLLEVPKSLNETRVADYLTLMLFDAEMTFYEQIVRLPPAHRMTVSQNSLDISAYWSLDPDFELQLDSDQAYAEQ
FQSIFTEAVRCRLRSAFSIGTMLSGGIDSSSITCMTAQLRQEAQETAKFPTFSAVFNEITASDEQEFMNAVIHQRPIEPQ
YVQGDQLSPWTDYQHMLAIQDEPLFACNAHLTHALYQIAQSQSVRVLLDGFDGDQAVSHGVGYLRTLAKAGRWLKLWREM
RGFCQSFGYDLWPLYRSYVRKFGINPLLKKTKITKILGKVKSWQFRGQQKTSEVTPARPWAIPLTPDFVDRASVLQRYKH
LRRQRFGAQPSQRHEHIVDVTSGLVSSTLEVMDRTAAASQVELRFPFWDKRLVEFCVSLPEEQKMQNGWTRLIIRRGLDG
ILPPQIQWRAGKGNLGTAFDHTLEKYGMDLIHQVLNHPPDAIETFIDMHEFTQLVQRWQRGEAEANDTLLIWIAVNLTLW
LQQANLGVNFAESTLQ
>Mature_655_residues
SAITGFVYPHPHPEGSDYLHQMVQSLSHRGPDGTNEWSQDRVGLGHCLLQTTPESRFETLPLSNASQTLILTADARIDNR
VDLMTQLGLKESPSAVITDSALILAAYEKWGEQCPEYFLGDFAFALWDETQQHLFCARDHFGVKPFFYYASEQVFAFASE
MKALFHLLEVPKSLNETRVADYLTLMLFDAEMTFYEQIVRLPPAHRMTVSQNSLDISAYWSLDPDFELQLDSDQAYAEQF
QSIFTEAVRCRLRSAFSIGTMLSGGIDSSSITCMTAQLRQEAQETAKFPTFSAVFNEITASDEQEFMNAVIHQRPIEPQY
VQGDQLSPWTDYQHMLAIQDEPLFACNAHLTHALYQIAQSQSVRVLLDGFDGDQAVSHGVGYLRTLAKAGRWLKLWREMR
GFCQSFGYDLWPLYRSYVRKFGINPLLKKTKITKILGKVKSWQFRGQQKTSEVTPARPWAIPLTPDFVDRASVLQRYKHL
RRQRFGAQPSQRHEHIVDVTSGLVSSTLEVMDRTAAASQVELRFPFWDKRLVEFCVSLPEEQKMQNGWTRLIIRRGLDGI
LPPQIQWRAGKGNLGTAFDHTLEKYGMDLIHQVLNHPPDAIETFIDMHEFTQLVQRWQRGEAEANDTLLIWIAVNLTLWL
QQANLGVNFAESTLQ

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:
GO:0004066: Asparagine synthase (Glutamine-hydrolyzing)
GO:0006529: Asparagine synthase (Glutamine-hydrolyzing)

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=272, Percent_Identity=27.5735294117647, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 74979; Mature: 74848

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAITGFVYPHPHPEGSDYLHQMVQSLSHRGPDGTNEWSQDRVGLGHCLLQTTPESRFET
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEECCCHHHHCC
LPLSNASQTLILTADARIDNRVDLMTQLGLKESPSAVITDSALILAAYEKWGEQCPEYFL
CCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
GDFAFALWDETQQHLFCARDHFGVKPFFYYASEQVFAFASEMKALFHLLEVPKSLNETRV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ADYLTLMLFDAEMTFYEQIVRLPPAHRMTVSQNSLDISAYWSLDPDFELQLDSDQAYAEQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCEEEEECCCHHHHHH
FQSIFTEAVRCRLRSAFSIGTMLSGGIDSSSITCMTAQLRQEAQETAKFPTFSAVFNEIT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ASDEQEFMNAVIHQRPIEPQYVQGDQLSPWTDYQHMLAIQDEPLFACNAHLTHALYQIAQ
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCEEHHHHHHHHHHHHHC
SQSVRVLLDGFDGDQAVSHGVGYLRTLAKAGRWLKLWREMRGFCQSFGYDLWPLYRSYVR
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KFGINPLLKKTKITKILGKVKSWQFRGQQKTSEVTPARPWAIPLTPDFVDRASVLQRYKH
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
LRRQRFGAQPSQRHEHIVDVTSGLVSSTLEVMDRTAAASQVELRFPFWDKRLVEFCVSLP
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHCCC
EEQKMQNGWTRLIIRRGLDGILPPQIQWRAGKGNLGTAFDHTLEKYGMDLIHQVLNHPPD
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
AIETFIDMHEFTQLVQRWQRGEAEANDTLLIWIAVNLTLWLQQANLGVNFAESTLQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECHHHHEECCCCCCCHHHHHCC
>Mature Secondary Structure 
SAITGFVYPHPHPEGSDYLHQMVQSLSHRGPDGTNEWSQDRVGLGHCLLQTTPESRFET
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEECCCHHHHCC
LPLSNASQTLILTADARIDNRVDLMTQLGLKESPSAVITDSALILAAYEKWGEQCPEYFL
CCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
GDFAFALWDETQQHLFCARDHFGVKPFFYYASEQVFAFASEMKALFHLLEVPKSLNETRV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ADYLTLMLFDAEMTFYEQIVRLPPAHRMTVSQNSLDISAYWSLDPDFELQLDSDQAYAEQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCEEEEECCCHHHHHH
FQSIFTEAVRCRLRSAFSIGTMLSGGIDSSSITCMTAQLRQEAQETAKFPTFSAVFNEIT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ASDEQEFMNAVIHQRPIEPQYVQGDQLSPWTDYQHMLAIQDEPLFACNAHLTHALYQIAQ
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCEEHHHHHHHHHHHHHC
SQSVRVLLDGFDGDQAVSHGVGYLRTLAKAGRWLKLWREMRGFCQSFGYDLWPLYRSYVR
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KFGINPLLKKTKITKILGKVKSWQFRGQQKTSEVTPARPWAIPLTPDFVDRASVLQRYKH
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHH
LRRQRFGAQPSQRHEHIVDVTSGLVSSTLEVMDRTAAASQVELRFPFWDKRLVEFCVSLP
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHCCC
EEQKMQNGWTRLIIRRGLDGILPPQIQWRAGKGNLGTAFDHTLEKYGMDLIHQVLNHPPD
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
AIETFIDMHEFTQLVQRWQRGEAEANDTLLIWIAVNLTLWLQQANLGVNFAESTLQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECHHHHEECCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]