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The map label for this gene is bioB

Identifier: 158334896

GI number: 158334896

Start: 1719168

End: 1720208

Strand: Direct

Name: bioB

Synonym: AM1_1733

Alternate gene names: 158334896

Gene position: 1719168-1720208 (Clockwise)

Preceding gene: 158334895

Following gene: 158334898

Centisome position: 26.43

GC content: 49.28

Gene sequence:

>1041_bases
TTGTCAGAAGAGACTTTCCCCCAAGCTTCAGCCTTAACCCTATTATCGGACCCCAATATCGACCTGCTGTCCTTGGTTGC
GGCAGCCGGAGAGGTGCGCATGACCTATTTTGGTCGGCAAGTAATGCTACACCGCATCAATGACATTCAAAACGGCCTCT
GCCCAGAAGATTGTGGCTATTGCGCCCAGTCTAAAATTTCCGATGCCCCGATCAAAAAATATCCTCTCAAAAGCGAAGAG
GACATTATCCAAGAGGCCTATGAAGCCAAAGCGAAGGGGGTATATCGCTACTGCATGGTGTCGAGCGGTCGAGGGCCAAC
TGCCGAACGGACCGAGCATTTAGCCCATATTATTCGCCGCATCAAAAATGAAGTGGGGATCCAAACCTGTCTTTCTGCCG
GTTTAATGGACCACGAACAGGCTGCTGTCCTCAAGGAAGCAGGACTAGACCGTCTCAATCACAATCTCAATACCAGCGAG
TCACACACGCCTGATATTGTCACCACCCATACGTTCCAAGATCGGATTAACACCTTAAAAGCAGCTCGGTCAGCTGGACT
AGATTTGTGTAGCGGCATGATTGCAGGTATGGGCGAAACGGACCAGGATATCGTTGACATAGCCTATCAACTGCATGAGT
ACCAGGTCCCCTCGATTCCCATTAACTTCCTGATACCGATTTCTGGGAATCCCATTTATGACTGTAACCAGCTCACGCCT
CAACGCTGCTTACGGATTCTGTGTCTATTTCGATTCGTGAACCCCAAAGCTGAGATTCGCATTGGAGGTGGCAGAGAAGG
GCACTTAAGGAGCTTGCAAGCTCTGGCACTTTACCCCGCCAACTCTCTTTTTGTGGAAGGATATCTAGCCACAAGAGGCC
ATAGTGTCGACCAAGTCTACCAGCTCATTCATGATGCAGGTTTCGAGGTTGCCGGAGAGACTTCGTTGACGGGCGATCTG
AGTGCAACATCCCAATTTCAGTTGGATGACAATCCCAACATTTTGAATCCACAAACCACCATCAGTTTCTCAAACTCATA
A

Upstream 100 bases:

>100_bases
CAGACCGGATACGCAATAGGATATTCTGACTAAGTTACACCGTTAGGGAAATCAGAGGTCAAGTATGGGCAGCGATTTCA
GTAAGTGGGTCAGAATCGCC

Downstream 100 bases:

>100_bases
GGGAAAAACATTAATCAACAGAAATGCCTTGAAGACTTTTTCTTCAAGGCATTTATTAAGGATCGAATTTTGATCCACAA
CCTGAATGGTCGAATATAAT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSEETFPQASALTLLSDPNIDLLSLVAAAGEVRMTYFGRQVMLHRINDIQNGLCPEDCGYCAQSKISDAPIKKYPLKSEE
DIIQEAYEAKAKGVYRYCMVSSGRGPTAERTEHLAHIIRRIKNEVGIQTCLSAGLMDHEQAAVLKEAGLDRLNHNLNTSE
SHTPDIVTTHTFQDRINTLKAARSAGLDLCSGMIAGMGETDQDIVDIAYQLHEYQVPSIPINFLIPISGNPIYDCNQLTP
QRCLRILCLFRFVNPKAEIRIGGGREGHLRSLQALALYPANSLFVEGYLATRGHSVDQVYQLIHDAGFEVAGETSLTGDL
SATSQFQLDDNPNILNPQTTISFSNS

Sequences:

>Translated_346_residues
MSEETFPQASALTLLSDPNIDLLSLVAAAGEVRMTYFGRQVMLHRINDIQNGLCPEDCGYCAQSKISDAPIKKYPLKSEE
DIIQEAYEAKAKGVYRYCMVSSGRGPTAERTEHLAHIIRRIKNEVGIQTCLSAGLMDHEQAAVLKEAGLDRLNHNLNTSE
SHTPDIVTTHTFQDRINTLKAARSAGLDLCSGMIAGMGETDQDIVDIAYQLHEYQVPSIPINFLIPISGNPIYDCNQLTP
QRCLRILCLFRFVNPKAEIRIGGGREGHLRSLQALALYPANSLFVEGYLATRGHSVDQVYQLIHDAGFEVAGETSLTGDL
SATSQFQLDDNPNILNPQTTISFSNS
>Mature_345_residues
SEETFPQASALTLLSDPNIDLLSLVAAAGEVRMTYFGRQVMLHRINDIQNGLCPEDCGYCAQSKISDAPIKKYPLKSEED
IIQEAYEAKAKGVYRYCMVSSGRGPTAERTEHLAHIIRRIKNEVGIQTCLSAGLMDHEQAAVLKEAGLDRLNHNLNTSES
HTPDIVTTHTFQDRINTLKAARSAGLDLCSGMIAGMGETDQDIVDIAYQLHEYQVPSIPINFLIPISGNPIYDCNQLTPQ
RCLRILCLFRFVNPKAEIRIGGGREGHLRSLQALALYPANSLFVEGYLATRGHSVDQVYQLIHDAGFEVAGETSLTGDLS
ATSQFQLDDNPNILNPQTTISFSNS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=303, Percent_Identity=33.6633663366337, Blast_Score=174, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6321725, Length=301, Percent_Identity=31.5614617940199, Blast_Score=159, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_ACAM1 (B0CC69)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516068.1
- ProteinModelPortal:   B0CC69
- SMR:   B0CC69
- GeneID:   5680549
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1733
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK960888
- BioCyc:   AMAR329726:AM1_1733-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 38084; Mature: 37952

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEETFPQASALTLLSDPNIDLLSLVAAAGEVRMTYFGRQVMLHRINDIQNGLCPEDCGY
CCCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCH
CAQSKISDAPIKKYPLKSEEDIIQEAYEAKAKGVYRYCMVSSGRGPTAERTEHLAHIIRR
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHH
IKNEVGIQTCLSAGLMDHEQAAVLKEAGLDRLNHNLNTSESHTPDIVTTHTFQDRINTLK
HHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHH
AARSAGLDLCSGMIAGMGETDQDIVDIAYQLHEYQVPSIPINFLIPISGNPIYDCNQLTP
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCH
QRCLRILCLFRFVNPKAEIRIGGGREGHLRSLQALALYPANSLFVEGYLATRGHSVDQVY
HHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHEECCCCCEEEEEEHHCCCCCHHHHH
QLIHDAGFEVAGETSLTGDLSATSQFQLDDNPNILNPQTTISFSNS
HHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEECCCEEEEECCC
>Mature Secondary Structure 
SEETFPQASALTLLSDPNIDLLSLVAAAGEVRMTYFGRQVMLHRINDIQNGLCPEDCGY
CCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCH
CAQSKISDAPIKKYPLKSEEDIIQEAYEAKAKGVYRYCMVSSGRGPTAERTEHLAHIIRR
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHH
IKNEVGIQTCLSAGLMDHEQAAVLKEAGLDRLNHNLNTSESHTPDIVTTHTFQDRINTLK
HHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHH
AARSAGLDLCSGMIAGMGETDQDIVDIAYQLHEYQVPSIPINFLIPISGNPIYDCNQLTP
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCH
QRCLRILCLFRFVNPKAEIRIGGGREGHLRSLQALALYPANSLFVEGYLATRGHSVDQVY
HHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHEECCCCCEEEEEEHHCCCCCHHHHH
QLIHDAGFEVAGETSLTGDLSATSQFQLDDNPNILNPQTTISFSNS
HHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA