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The map label for this gene is gutB [H]

Identifier: 158334693

GI number: 158334693

Start: 1509610

End: 1510656

Strand: Reverse

Name: gutB [H]

Synonym: AM1_1526

Alternate gene names: 158334693

Gene position: 1510656-1509610 (Counterclockwise)

Preceding gene: 158334694

Following gene: 158334692

Centisome position: 23.23

GC content: 50.62

Gene sequence:

>1047_bases
ATGAAAGCTCAAGTCTTTCGCGGGGTTAATCAACTCAGCTACGAAGAGATACCAAAGCCTGAAGTGGGTGCAGATGAGGT
GCTCGTCCAAGTCAAAGTAGTCGGTCTTTGTCAATCCGACATTAAAAAAATCCGCTATCCCCTCTACGAGCCACCCCGGA
TATTTGGCCACGAAACGGCAGGCGAGATTGCCGCTGTGGGTTCTGAGGTTAAAGGCTGGCAGGTGGGGCAACGGGTGGTT
GTGCTGCACCATATTCCCTGTATGCACTGCGACTACTGTCTGAATGATAACTTCTCCATGTGTGAGGTGTATAAAAACAT
CACCACCACGGCAGGATTTAGCCCGAGCGGCGGCGGCTTTGCCGAATATGTAAAGGTTCCGGGCCATATCGTTACCCGAG
GCGGATTAATTGCTATTCCCGAAGATATTTCTTTTGAGCAAGCGAGCTTTGTTGAACCCACCAACTGCTGTTTGAAGGCC
GTCACCAAAGCCGATATCAAACCGGGACAAACGGTCCTGATCACGGGGGCTGGCCCCATTGGCCTGATGTTCGTGATGCT
CGTCAACTATGTGGGCGGTCGTGCCATCGCCACGGATCTCTTACCCACGCGAATTGAAAAAGCGAAGCAGGTGGGAGCGA
CGGCGGCCTTTGACGCTCGCGATCCCGATCTAGCCTCCAAAATTCAAGATTTGACCAATGGTATGGGGGTGGATGTCAGC
TTACTAGCCGTGCCCAGCGATAAAGCCTTTTTCCAAGCCCTCGACTGTACCCGCAAAGGCGGTAAGATTCTGTTCTTTGC
TGAGTTCCCCGATGAAGTCGAAATTCCTATTAATCCCAATGTCTTGTATCGTCGCGAAATCGATTTGATTGGCAGCTATA
GCTCTTCTTTTCGATTACAGAAACAGTCGGCGGACATTATTTTCAACAAGCGAATTGATGTCGATGCCTTAGTCAGCGAT
CGCTATCCGCTTCAAGACTTAGCGAATGCGGTCGAGCAGGCGGTTTCTCCCGGACCTGAGACCTACAAGATTTTAATTTA
TCCCTAA

Upstream 100 bases:

>100_bases
TTTTTTAATGAAACCCTGCCTGCTGGTGGGGTTTCATGGCTTGAGGGACAGGAAGCAACACGATAAGCTGAAAACAGACG
TTTGTTAGGGGTCGGGAAGC

Downstream 100 bases:

>100_bases
GAAGCATTTAATTGGAGCAAAGGGACCACCAAGCGATACGATCAGGATTGCTGAATCACGTTGCTCCCTTCTATGCCTGC
TGTTCATATTATTGGTCTTG

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKAQVFRGVNQLSYEEIPKPEVGADEVLVQVKVVGLCQSDIKKIRYPLYEPPRIFGHETAGEIAAVGSEVKGWQVGQRVV
VLHHIPCMHCDYCLNDNFSMCEVYKNITTTAGFSPSGGGFAEYVKVPGHIVTRGGLIAIPEDISFEQASFVEPTNCCLKA
VTKADIKPGQTVLITGAGPIGLMFVMLVNYVGGRAIATDLLPTRIEKAKQVGATAAFDARDPDLASKIQDLTNGMGVDVS
LLAVPSDKAFFQALDCTRKGGKILFFAEFPDEVEIPINPNVLYRREIDLIGSYSSSFRLQKQSADIIFNKRIDVDALVSD
RYPLQDLANAVEQAVSPGPETYKILIYP

Sequences:

>Translated_348_residues
MKAQVFRGVNQLSYEEIPKPEVGADEVLVQVKVVGLCQSDIKKIRYPLYEPPRIFGHETAGEIAAVGSEVKGWQVGQRVV
VLHHIPCMHCDYCLNDNFSMCEVYKNITTTAGFSPSGGGFAEYVKVPGHIVTRGGLIAIPEDISFEQASFVEPTNCCLKA
VTKADIKPGQTVLITGAGPIGLMFVMLVNYVGGRAIATDLLPTRIEKAKQVGATAAFDARDPDLASKIQDLTNGMGVDVS
LLAVPSDKAFFQALDCTRKGGKILFFAEFPDEVEIPINPNVLYRREIDLIGSYSSSFRLQKQSADIIFNKRIDVDALVSD
RYPLQDLANAVEQAVSPGPETYKILIYP
>Mature_348_residues
MKAQVFRGVNQLSYEEIPKPEVGADEVLVQVKVVGLCQSDIKKIRYPLYEPPRIFGHETAGEIAAVGSEVKGWQVGQRVV
VLHHIPCMHCDYCLNDNFSMCEVYKNITTTAGFSPSGGGFAEYVKVPGHIVTRGGLIAIPEDISFEQASFVEPTNCCLKA
VTKADIKPGQTVLITGAGPIGLMFVMLVNYVGGRAIATDLLPTRIEKAKQVGATAAFDARDPDLASKIQDLTNGMGVDVS
LLAVPSDKAFFQALDCTRKGGKILFFAEFPDEVEIPINPNVLYRREIDLIGSYSSSFRLQKQSADIIFNKRIDVDALVSD
RYPLQDLANAVEQAVSPGPETYKILIYP

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=339, Percent_Identity=25.0737463126844, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI71565152, Length=370, Percent_Identity=26.7567567567568, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI156523966, Length=348, Percent_Identity=26.1494252873563, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4501939, Length=348, Percent_Identity=26.1494252873563, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4501933, Length=341, Percent_Identity=26.3929618768328, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI4501929, Length=360, Percent_Identity=25.5555555555556, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI71565154, Length=346, Percent_Identity=26.0115606936416, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI262073058, Length=344, Percent_Identity=24.1279069767442, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI71743840, Length=344, Percent_Identity=24.1279069767442, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1788073, Length=340, Percent_Identity=30, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI226510992, Length=335, Percent_Identity=27.7611940298507, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790045, Length=338, Percent_Identity=26.0355029585799, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788075, Length=331, Percent_Identity=26.5861027190332, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082125, Length=349, Percent_Identity=27.7936962750716, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788407, Length=339, Percent_Identity=27.4336283185841, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787863, Length=224, Percent_Identity=34.8214285714286, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786825, Length=307, Percent_Identity=30.6188925081433, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790718, Length=313, Percent_Identity=25.5591054313099, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081918, Length=271, Percent_Identity=27.6752767527675, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082401, Length=353, Percent_Identity=27.1954674220963, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=340, Percent_Identity=27.6470588235294, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17562878, Length=342, Percent_Identity=26.3157894736842, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17562584, Length=306, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17562582, Length=325, Percent_Identity=28, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71988145, Length=315, Percent_Identity=25.7142857142857, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25146526, Length=361, Percent_Identity=23.8227146814404, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71997431, Length=363, Percent_Identity=23.6914600550964, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565904, Length=314, Percent_Identity=23.8853503184713, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319955, Length=358, Percent_Identity=25.1396648044693, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6322619, Length=358, Percent_Identity=25.1396648044693, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323099, Length=325, Percent_Identity=26.7692307692308, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6319258, Length=223, Percent_Identity=31.8385650224215, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319257, Length=384, Percent_Identity=25, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6319621, Length=244, Percent_Identity=31.1475409836066, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323729, Length=237, Percent_Identity=28.2700421940928, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323961, Length=315, Percent_Identity=25.3968253968254, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324486, Length=263, Percent_Identity=26.9961977186312, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6319520, Length=261, Percent_Identity=24.904214559387, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320033, Length=353, Percent_Identity=22.9461756373938, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17137530, Length=352, Percent_Identity=27.5568181818182, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI17737897, Length=352, Percent_Identity=28.4090909090909, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17737895, Length=350, Percent_Identity=24.5714285714286, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 37989; Mature: 37989

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAQVFRGVNQLSYEEIPKPEVGADEVLVQVKVVGLCQSDIKKIRYPLYEPPRIFGHETA
CCCHHHHCHHHHHHHHCCCCCCCHHHEEEEEEEEHHCHHHHHHHCCCCCCCCHHCCCCCC
GEIAAVGSEVKGWQVGQRVVVLHHIPCMHCDYCLNDNFSMCEVYKNITTTAGFSPSGGGF
CHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCH
AEYVKVPGHIVTRGGLIAIPEDISFEQASFVEPTNCCLKAVTKADIKPGQTVLITGAGPI
HHHEECCCEEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHH
GLMFVMLVNYVGGRAIATDLLPTRIEKAKQVGATAAFDARDPDLASKIQDLTNGMGVDVS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCEE
LLAVPSDKAFFQALDCTRKGGKILFFAEFPDEVEIPINPNVLYRREIDLIGSYSSSFRLQ
EEEECCCHHHHHHHHHHCCCCEEEEEEECCCCEECCCCCCEEEEEEEEEEECCCCCEEEE
KQSADIIFNKRIDVDALVSDRYPLQDLANAVEQAVSPGPETYKILIYP
CCCCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure
MKAQVFRGVNQLSYEEIPKPEVGADEVLVQVKVVGLCQSDIKKIRYPLYEPPRIFGHETA
CCCHHHHCHHHHHHHHCCCCCCCHHHEEEEEEEEHHCHHHHHHHCCCCCCCCHHCCCCCC
GEIAAVGSEVKGWQVGQRVVVLHHIPCMHCDYCLNDNFSMCEVYKNITTTAGFSPSGGGF
CHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCH
AEYVKVPGHIVTRGGLIAIPEDISFEQASFVEPTNCCLKAVTKADIKPGQTVLITGAGPI
HHHEECCCEEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHH
GLMFVMLVNYVGGRAIATDLLPTRIEKAKQVGATAAFDARDPDLASKIQDLTNGMGVDVS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCEE
LLAVPSDKAFFQALDCTRKGGKILFFAEFPDEVEIPINPNVLYRREIDLIGSYSSSFRLQ
EEEECCCHHHHHHHHHHCCCCEEEEEEECCCCEECCCCCCEEEEEEEEEEECCCCCEEEE
KQSADIIFNKRIDVDALVSDRYPLQDLANAVEQAVSPGPETYKILIYP
CCCCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]